(DRAFT) - Taxonomy
                                Species TOAD, HOUSTON
                                Species Id ESIS202001
                                   Date 14 MAR 96



TAXONOMY

NAME - TOAD, HOUSTON OTHER COMMON NAMES - TOAD and HOUSTON ELEMENT CODE - CATEGORY - Amphibians PHYLUM AND SUBPHYLUM - CHORDATA, CLASS AND SUBCLASS - AMPHIBIA, ORDER AND SUBORDER - SALIENTIA, FAMILY AND SUBFAMILY - BUFONIDAE, GENUS AND SUBGENUS - BUFO, SPECIES AND SSP - HOUSTONENSIS, SCIENTIFIC NAME - BUFO HOUSTONENSIS AUTHORITY - TAXONOMY REFERENCES - COMMENTS ON TAXONOMY - Houston Toad Bufo houstonensis Sanders, 1953 KINGDOM: Animal GROUP: Amphibian PHYLUM: Chordata CLASS: Amphibia ORDER: Salientia FAMILY: Bufonidae Adult Houston toads are small to medium toads with males between 45 and 70 mm snout-vent length and females 52-80 mm. The dorsum is light brown with a variable number of dark brown to black spots. These spots usually contain a single, or several fused, nonspinous warts and the spot may appear as a narrow black margin around the warts. The venter is cream colored with at least one brown spot in the pectoral region (usually mottled). The parotoid glands are elongate but otherwise variable in shape. The interorbital and postorbital cranial crests are typically thickened. But this character is much more obvious in the type series than in extant individuals (01). Within the jelly tube, the eggs are separated from one another in compartments. For a more extensive description of the Houston toad see Sanders (02) and Brown (03). Photographs of adult B. houstonensis are in Sanders (02) and various other publications cited herein Taxonomy - 1 (DRAFT) - Taxonomy Species TOAD, HOUSTON Species Id ESIS202001 Date 14 MAR 96 (05,13,12). The tadpoles of the Houston toad are quite distinct from all sympatric anuran species but visually inseparable from those of B. americanus. The body and upper 3/4 of the tail are thoroughly pigmented. Total length of 15 specimens examined (stages 33-42 of Gosner (04)) ranged 13.7-19.4 mm (X=17.3 mm). The labial formula is is 2(2)/3. Bufo houstonensis was first described by Sanders (02) in 1953. There has been some discussion as to its distinct differences from other bufonids and that natural hybridization may be a cause of a trend toward extinction. Little evidence indicates that natural hybridization has such an effect. Only 34 confirmed natural hybrids (25 B. houstonensis X B. valliceps, 9 B. woodhousei) have been identified (05,06). No evidence indicates widespread hybridization, back-crossing, or introgression. Hillis et. al. (06) found that hybrids make up less than 1 percent of toad breeding choruses involving B. houstonensis and another Bufo species. Brown (05) only indicated that natural hybridization potentially could contribute to the trend toward extinction. Houston populations of B. houstonensis were originally called B. terrestris by Harwood (24 in 01). Sanders (24 in 01) used the Houston area toads as a basis for the description of B. houstonensis, saying they differed from B. americanus in color pattern, skeletal morphology, and the presence of "egg compartments." Brown (05 in 01) showed that the call of the Houston toad differed from that of New Jersey B. americanus. A. P. Blair (23 in 01) considered the Houston toad to be a subspecies of B. americanus but all recent authors have considered it a full species. W. F. Blair (08 in 01) suggests that it represents a slightly differentiated Pleistocene relic of B. americanus. Due to the general acceptance of the latter theory, many herpetologists feel that the relationships of B. houstonensis to nearby populations of B. americanus have not been adequately studied and are not well understood. Taxonomy - 2
                                  (DRAFT) - Status
                                Species TOAD, HOUSTON
                                Species Id ESIS202001
                                   Date 14 MAR 96



STATUS

Coded Status E: Federal Endangered COMMENTS ON STATUS - U.S. STATUSES AND LAWS: The Houston toad (Bufo houstonensis) has been designated an Endangered species pursuant to the Endangered Species Act of 1973 (50 CFR 17.11; P.L. 93-205, 87 Stat. 884; 16 U.S.C. 1531-1540), as amended. The species has this status wherever found including the State of Texas. Critical Habitat has been designated in areas of Burleson and Bastrop Counties, Texas (50 CFR 17.95(d)). This species is protected by the Lacey Act (P.L. 97-79, as amended; 16 U.S.C. 3371 et seq.) which makes it unlawful to import, export, transport, sell, receive, acquire, or purchase any wild animal (alive or dead including parts, products, eggs, or offspring): (1) in interstate or foreign commerce if taken, possessed, transported or sold in violation of any State law or regulation, or foreign law; or (2) if taken or possessed in violation of any U.S. law, treaty, or regulation or in violation of Indian tribal law. It is also unlawful to possess any wild animal (alive or dead including parts, products, eggs, and offspring) within the U.S. territorial or special maritime jurisdiction (as defined in 18 U.S.C. 7) that is taken, possessed, transported, or sold in violation of any State law or regulation, foreign law, or Indian tribal law. RESPONSIBLE FEDERAL AGENCIES: USFWS -Responsible for the management/recovery, listing, and law enforcement/protection of this species. DOD -Responsible for the law enforcement/protection of this species with applicable State and Federal laws on public land under their control. Also responsible for management/recovery on Department of Defense lands. All Federal agencies have responsibility to ensure that any action authorized, funded, or carried out by that agency is not likely to jeopardize the continued existence of the species or result in the destruction or adverse modification of Critical Habitat (50 CFR 402), and to utilize their authorities to carry out programs for the conservation of the species. STATE STATUSES AND LAWS: Status - 1 (DRAFT) - Status Species TOAD, HOUSTON Species Id ESIS202001 Date 14 MAR 96 STATE: Texas DESIGNATED STATUS: Endangered ADMINISTRATIVE AGENCY: Texas Parks and Wildlife Department STATE STATUTE: TX Parks and Wildlife Codes 127.30.09.001-.006 INTERNATIONAL STATUSES, TREATIES, AND AGREEMENTS: The Houston toad is listed in the 1986 IUCN Red List of Threatened Animals as Endangered. It is also listed by the U.S. in the Convention on Nature Protection and Wildlife Preservations in the Western Hemisphere Annex (1970). ECONOMIC STATUSES: This species has no known economic value. 70/07/25:35 FR 13519/13520 - Listed as Endangered, Pre-Act 6 70/10/13:35 FR 16047/16048 - Re-listed, Pre-Act 7 78/01/31:43 FR 04022/ - Designated Critical Habitat 79/05/21:44 FR 29566/29577 - 5-year review 85/07/22:50 FR 29901/29909 - Notice of review 87/07/07:52 FR 25522/25525 - Notice of 5-year review completion Status - 2
     

HABITAT ASSOCIATIONS

HABITAT - AQUATIC TERRESTRIAL INLAND AQUATIC SOCIETY OF AMERICAN FORESTRY TYPES SAF TYPE STAGE CLOSURE Loblolly Pine-Shortleaf Pine young tree Loblolly Pine-Shortleaf Pine mature tree Loblolly Pine-Shortleaf Pine Old Growth LAND USE - Residential Transportation, communications, and Util Mixed Urban or Built-up Land Cropland and Pasture Evergreen Forest Land Lakes Forested Wetland Sandy Areas other than Beaches NATIONAL WETLAND INVENTORY CODES NWI NWICLS NWIMOD NWISPEC Palustrine UB Palustrine SS7 Palustrine OW0 Palustrine EM COMMENTS ON HABITAT ASSOCIATIONS - Water is one of the most important parts of Houston toad habitat as reproduction cannot occur if there is not enough water to fill breeding pools. The largest aggregations of calling and breeding Houston toads are found in temporary rain pools. They may, however, be found in a variety of aquatic habitats. The area surrounding the primary calling site in Burleson County had been bulldozed and is grazed by cattle; the pool is surrounded by a minimally 10 m wide strip of thick yaupon (Ilex vomitoria) growth. Other calling sites include waters associated with open pasture, cleared shallow ravines, the vicinity of dwellings around a lake, roadside ditches, lakes, manmade ponds, cleared areas within the pine forest, pastures, flooded fields, puddles near cabins, moist spots in residential areas, prairie potholes, and near runways on Ellington AFB. Water is also important after breeding as tadpoles will die if pools dry up before metamorphosis. Adults also require free water for metabolism and normal functions (01). Houston toads apparently are restricted to areas characterized by sandy soils. B. houstonensis is a weak burrower and has difficulty digging in compacted soil (11). Six Houston toads kept in the laboratory spent most of the daylight hours buried under sand. A sand substrate occurs at or near all known localities for B. houstonensis (05). Upon leaving the breeding pond, toads seek refuge in areas with sand substrate in such places as under logs, in leaf litter, existing burrows, undercut banks around ponds,and in the actual sand substrate. There has been a misconceived assumption that pine trees occur naturally at all Houston toad localities. A great abundance of natural loblolly pine (Pinus taeda) usually characterizes the Habitat Associations - 1 localities in Bastrop County and pines are found at or near some other localities for B. houstonensis. However, the pine stand at Lake Woodrow (Burleson County) was planted in 1959 (12). Also, the habitats at the Skyscraper Shadows/East Haven and Fairbanks localities (Harris County) were coastal prairie without pines before being developed for housing. However, a common characteristic of all known localities for Houston toads is a friable, sandy soil (see above, also 03,13) which, coincidentally, is conducive to pine growth. Thus the toads are probably reliant upon the substrate but not on the presence of pine trees. High temperature may also be a factor. Blair (08) considered B. houstonensis to be a weakly differentiated species that originated after isolation from northern adapted B. americanus after the Pleistocene. Brown (09) suggested that B. houstonensis may still be a northern adapted species and its inability to adapt to a warmer climate could be contributing to the trend toward extinction. Lundelius (10) also suggested that higher temperatures and/or aridity were the cause(s) of disappearance from Texas of a number of northern adapted species of mammals in the last 10,000 years. Habitat Associations - 2
                                (DRAFT) - Food Habits
                                Species TOAD, HOUSTON
                                Species Id ESIS202001
                                   Date 14 MAR 96



FOOD HABITS

TROPHIC LEVEL - OMNIVORE LIFESTAGE FOOD FOOD PART General Algae General Arthropods Food Habits - 1
                         (DRAFT) - Environment Associations
                                Species TOAD, HOUSTON
                                Species Id ESIS202001
                                   Date 14 MAR 96



ENVIRONMENTAL ASSOCIATIONS

G = General A = Adult LIM = Limiting RA = Resting Adult J = Juvenile FA = Feeding Adult RJ = Resting Juvenile BA = Breeding Adult FJ = Feeding Juvenile P = Pupae L = Larvae E = Egg RL = Resting Larvae FL = Feeding Larvae
LIFESTAGE ENVIRONMENTAL ASSOCIATIONS G Terrestrial Features: Depressions G Terrestrial Features: Burrows G Terrestrial Features: Downed logs G Human Association: Farm ponds G Environment Associations - 1
                               (DRAFT) - Life History
                                Species TOAD, HOUSTON
                                Species Id ESIS202001
                                   Date 14 MAR 96



LIFE HISTORY

FOOD HABITS: Bragg (11) reported that captive Houston toads fed on various insects, a small spadefoot toad, and presumably a small Bufo cognatus. Thomas (14) examined the digestive tracts of 17 adults; all were empty except one which was stuffed with ants (Crematogaster cf. minutissima, fide Mehlhop) and one which had beetle remains. Post metamorphic B. houstonensis presumably feeds on small arthropods and the tadpoles are known to ingest algae and pollen (14). PERIODICITY: Houston toads are primarily nocturnal, resting in burrows during the day in some secluded place with sand substrate in such places as under logs, in leaf litter, existing burrows, undercut banks around ponds, and in the actual sand substrate (01). MIGRATION PATTERNS: Houston toads are non-migratory. COVER/SHELTER REQUIREMENTS: Houston toads need sandy soils in which to burrow. They rest in burrows during the day in some secluded place with sand substrate in such places as under logs, in leaf litter, existing burrows, undercut banks around ponds, and in the actual sand substrate (01). REPRODUCTIVE SITE REQUIREMENTS: Houston toads appear generally to use rain pools or flooded fields for breeding, but they may use natural or manmade ponds. They are also found in a variety of aquatic habitats. The area surrounding the primary calling site in Burleson County had been bulldozed and is grazed by cattle; the pool is surrounded by a minimally 10 m wide strip of thick yaupon (Ilex vomitoria) growth. Other calling sites include waters associated with open pasture, cleared shallow ravines, the vicinity of dwellings around a lake, roadside ditches, lakes, manmade ponds, cleared areas within the pine forest, pastures, flooded fields, puddles near cabins, moist spots in residential areas, prairie potholes, and near runways on Ellington AFB (01). REPRODUCTIVE CHARACTERISTICS: Houston toads call from in or near the water, with the earliest calling date being January 22 (14). Reported egg laying dates range from February 18 to June 26 (06,13). Quinn (15) reported that captive raised males matured at one year, with active sperm present at eight months, when snout-vent lengths were 3.1 cm. Females matured sexually at 1-2 years. Kennedy (13) reported a female produced 728 eggs in the lab. Egg masses range from 513-5999 eggs from wild caught females spawned in the laboratory (16). PARENTAL CARE: Egg masses laid by the females are left unattended. The tadpoles are on their own from their hatching through metamorphosis. Life History - 1 (DRAFT) - Life History Species TOAD, HOUSTON Species Id ESIS202001 Date 14 MAR 96 POPULATION BIOLOGY: Limiting factors for Houston toads are friable, sandy substrates, open water for breeding, and habitat loss (see N-HABITAT and N-REASON narratives). Since the late 1940s, populations of Houston toads in Harris County have decreased markedly. Wottring's field notes (07) indicate that he collected 66 individuals from a single chorus in 1949 and still found "quite a lot" in 1953. Brown (05,09) found three Houston toads during the breeding season of 1965-67. After extensive surveys in the springs of 1974-78, only 2 toads were observed in SE Houston in 1976. Despite extensive surveillance, no indication exists of the Houston toad's presence in Harris County since that time. Burleson County has a small population of Houston toads near Lake Woodrow. This assessment is based on verbal comparisons of field studies by Brown (12 or fewer individuals) in the mid-1960s and Thomas (around 300 individuals) in the mid-1970s. Most observations were of one to several calling males. Jim R. Dixon (17) found no Houston toads during the springs of 1979-82, but in 1983 four calling males were observed. The population in Bastrop County has increased. Brown (18) reported that the population probably was represented by no more than 300 individuals in 1967. By the mid-1970s, Thomas and Porter (19) estimated 1500 individuals. During the breeding seasons of 1981-1982, Hillis et al. (06) and Jacobson (14) studied Houston toad populations with emphasis on several ponds north of Highway 21 in Bastrop County. They commonly encountered choruses of 30-100 individuals at study ponds. A mark and recapture program at one pond indicated that 50-75 percent of males on subsequent nights were new and that females were not observed returning to the ponds. Accordingly, these authors estimated 300-1000 toads using each pond. As the study area covered less than 5 percent of the known range in Bastrop County, the overall population may be large, perhaps 10,000 or more toads (20). Eggs, adults, and recently metamorphosed toads collected near Bastrop State Park and captive-reared at Houston Zoological Gardens were released in Attwater's Prairie Chicken NWR in the spring of 1983. A total of 24,702 individuals and 22 bloodlines were involved. Success of the transplant is yet unknown (21). SPECIES INTERRELATIONSHIPS: The following anurans frequent the same or adjacent calling sites as the Houston toad and may use the same resources, at various stages of their life history: Rana clamitans, R. sphenocephala, R. catesbeiana, R. areolata, Hyla crucifer, H. cinerea, H. chrysoscelis, H. squirella, H. versicolor, Acris crepitans, Scaphiopus holbrooki, Gastrophyryne olivacea, G. carolinensis, Pseudacris clarki, P. triseriata and P. streckeri. The only Bufo species known to be sympatric with Houston toad are B. valliceps and B. woodhousei, though B. speciosus and B. punctatus occur within 20 miles. For a significant portion of the breeding season, Houston toads are temporally isolated from B. valliceps. Some overlap occurs in April and May, but breeding site and feeding competition is probably minimal (03,04). The breeding season of Houston toads and Life History - 2 (DRAFT) - Life History Species TOAD, HOUSTON Species Id ESIS202001 Date 14 MAR 96 B. woodhousei is similar, but habitat segregation appears to be the rule (06). Hognose snakes (Heterodon nasicus and H. platyrhinos) feed on Houston toads and a Nerodia erythrogaster (red belly water snake) was collected that contained 2 Houston toads while swallowing another (20). Nerodia rhombifera (diamond back water snake), N. fasciata (banded water snake), and Thamnophis proximus (ribbon snake) are also potential predators. Other occasional predators could include Kinosternon subrum (mud turtle), K. flavescens (yellow mud turtle), Sternotherus odoratus (Stinkpot), and Chelydra serpentina (snapping turtle). Micropterus salmoides (largemouth bass), Lepomis sp. (sunfish) and other Piscine predators might feed on toad larvae or eggs; although closely related B. americanus tadpoles are relatively immune to fish predators (22). Warm-blooded associates include various birds and mammals. Those which are general predators may eat Houston toads. OTHER LIFE HISTORY DESCRIPTORS: None. Life History - 3
                           (DRAFT) - Management Practices
                                Species TOAD, HOUSTON
                                Species Id ESIS202001
                                   Date 14 MAR 96



MANAGEMENT PRACTICES

RESULT MANAGEMENT PRACTICE Beneficial Restricting/regulating human disturbance of populations Beneficial Maintaining undisturbed/undeveloped areas Beneficial Land Acquisition Beneficial Controlling pollution [thermal, chemical, physical] Beneficial Controlling/Restricting Pesticide Use Beneficial Controlling/Restricting Herbicide Use Beneficial Stocking captive-reared wild-strain animals Beneficial Transplanting wild animals Beneficial Transplanting Wild Eggs/Wild Seeds Adverse Climate Alteration Existing Climate Alteration Adverse Rural Residential/Industrial Areas Existing Rural Residential/Industrial Areas Adverse Highway/Railroads Existing Highway/Railroads Adverse Soil compaction by heavy equipment in mine areas Existing Soil compaction by heavy equipment in mine areas Adverse Strip mining Existing Strip mining Adverse Existing Adverse Fire Existing Fire Adverse Forest Alteration Existing Forest Alteration Adverse Harvesting Existing Harvesting COMMENTS ON MANAGEMENT PRACTICES - The most important causes of the decline of B. houstonensis are probably climatic changes and destruction and/or modification of habitats (01). Water is one of the most important factors limiting Houston toads as reproduction cannot occur if there is not enough water to fill breeding pools. During the early 1960s, spring rains in the Houston area were below normal, and no breeding choruses were heard (07). Droughts in the early and mid-1950s may have played an important role in reducing the numbers of the species. Water is also important after breeding as tadpoles will die if pools dry up before metamorphosis. Adults also require free water to survive (01). High temperature may also be a factor. Blair (08) considered B. houstonensis to be a weakly differentiated species that originated after isolation from northern adapted B. americanus after the Pleistocene. Brown (09) suggested that B. houstonensis may still be a northern adapted species and its inability to adapt to a warmer climate could be contributing to its trend toward extinction. Lundelius (10) also suggested that higher temperatures and/or aridity were the cause(s) of disappearance from Texas of a number of northern adapted species of mammals in the last 10,000 years. Management Practices - 1 (DRAFT) - Management Practices Species TOAD, HOUSTON Species Id ESIS202001 Date 14 MAR 96 There has been extensive habitat modification in the known present populations of Houston toads. In Harris County, the growing city of Houston has been consuming habitat rapidly. Development (primary recreational including golf courses, artificial lakes, and camping areas) of Bastrop and Buescher State Parks in the 1960s changed drainage patterns and adversely affected the toads as did lumbering (including timber clearing for housing development and agricultural use) and highway improvement projects (resulting in altered drainage pattern). Park management improved during the 1970s, as have other land management (reforesting, grazing) practices. At the Burleson County site the major problem is overgrazing by cattle which results in habitat alteration (01). In Bastrop County Highways 71 and 21 cut through pine forests subjecting B. houstonensis to traffic mortality. Periodic mowing along highway and on a golf course occasionally kill the species. Pesticides and herbicides (i.e., Atrazine) used along roads, by residents, or in surrounding croplands, may adversely impact the toad (01). Forest management such as prescribed burning to reduce undergrowth could be detrimental by direct mortality as well as reducing invertebrate food resources (01). Activities associated with urban and agricultural development cause soil compaction due to the use of heavy equipment and plowing, and will adversely impact the species (01). Since it is a weak burrower and has difficulty digging in compacted soil (01). A stratum of lignite crosses a portion of Critical Habitat in Bastrop County. If mining were to be considered in the future this could have deleterious habitat impacts (01). APPROVED PLAN: U.S. Fish and Wildlife Service. 1984. Houston Toad Recovery Plan. U.S. Fish and Wildl. Serv., Albuquerque, NM 73 pp. The first action planned in the recovery of the Houston toad (Bufo houstonensis) is to maintain and enhance existing toad populations in their existing habitats. This includes monitoring existing populations and habitats, identifying population needs and habitat needs, and assuring protection (i.e., controlling/restricting herbicide use, pesticide use, pollutants, and development; land acquisition; and limiting human access) of existing populations in Bastrop and Burleson Counties. There is a need to improve monitoring procedures and schedules and recommendations are made. There will also be an effort to locate additional natural populations of Houston toads, and to determine the taxonomic status of Bufo houstonensis. The plan calls for reestablishment (or locating presently unknown populations) of viable populations in at least 5 counties within the former range of the Houston toad, and monitoring of populations and managing of habitats in the new locales. These new populations will be established by: transplanting wild individuals and their eggs, and from individuals raised in a captive breeding program. Managing will involve cooperative agreements with landowners of new locales and reviews and comments on all projects which might impinge on existing or reestablished Houston toad populations. Introduced colonies will Management Practices - 2 (DRAFT) - Management Practices Species TOAD, HOUSTON Species Id ESIS202001 Date 14 MAR 96 be considered self-sustaining if they persist without any additional introductions for at least 10 years and if at least 100 calling males can be located within any single week during years 8, 9, or 10. In addition, the habitats of both existing and introduced populations have to be free of threats associated with physical, chemical, or biological modifications that might make the habitat unsuitable for the Houston toad. If, in the opinion of the recovery team, after consultation with herpetological systematists, B. houstonensis is synonymous with any other toad taxon, it should be reclassified to threatened status. Management Practices - 3
                                   (DRAFT) - References
                                  Species TOAD, HOUSTON
                                  Species Id ESIS202001
                                      Date 14 MAR 96



     

References

***** REFERENCES FOR ALL NARRATIVES EXCEPT N-OCCURRENCE ***** 01 U.S. Fish and Wildlife Service. 1984. Houston Toad Recovery Plan. U.S. Fish & Wildl. Serv., Albuquerque, NM 72 pp. 02 Sanders, O. 1953. A new species of toad, with discussion of morphology of the Bufonid skull. Herpetologica 9:25-47. 03 Brown, L.E. 1973. Bufo houstonensis. Cat. Amer. Amphib. Rept. 133.1-133.2. 04 Gosner, K.L. 1960. A simplified table for staging Anuran embryos and larvae with notes on identification. Herpetologica 16:183-190. 05 Brown, L.E. 1971. Natural hybridization and trends toward extinction in some relict texas toad populations. Southwest Nat. 16:185-199. 06 Hillis, D.M., A.M. Hillis, and R.F. Martin. In Press. Reproductive ecology and hybridization of the endangered Houston toad (Bufo houstonensis). 07 Wottring, J. Undated Pers. Comm., Cited in (01). 08 Blair, W.F. 1965. Amphibian speciation. pp. 543-556. In: H.E. Wright and D.G. Frey (eds.). The Quaternary of the United States. Princeton Univ. Press, Princeton, NJ. 09 Brown, L.E. 1967. The significance of natural hybridization in certain aspects of the speciation of some North American toads (Genus Bufo). Ph.D. Dissertation, Univ. Texas, Austin. X + 126 pp. 10 Lundelius, E.L.,Jr. 1967. Late-Pleistocene and Holocene faunal history of central Texas. pp. 287-319. In: P.S. Martin and H.E. Wright, Jr. (eds.), Pleistocene extinctions--The search for a cause. Yale Univ. Press, New Haven, CT. 11 Bragg, A.N. 1960. Feeding in the Houston toad. Southwest Nat. 5:106. 12 Thomas, R.A. 1977. The endangered Houston toad (Bufo houstonensis). Final Rept. to: USFWS, Contract No. 14-16-0002-3557, 11 pp. 13 Kennedy, J.P. 1962. Spawning season and experimental hybridization of the Houston toad, Bufo houstonensis. Herpetologica 17:239-245. 14 Jacobson, N.L. 1983. Reproductive behavior and male mating success in the Houston toad (Bufo houstonensis). Draft of M.S. Thesis, Univ. Texas, Austin. 54 pp. Files of USFWS, Albuquerque, NM. 15 Quinn, H. 1980. Final report of captive propagation/release of the Houston toad, Bufo houstonensis. Houston Zoological Gardens, Typescript, 14 pp. Files of USFWS, Albuquerque, NM. 16 Quinn, H. in Press. Reproduction and growth in Bufo houstonensis. Southwest Nat. 17 Dixon, J.R. Undated Pers. Comm. in (01). 18 Brown, L.E. 1975. The status of the near-extinct Houston toad (Bufo houstonensis) with recommendations for its conservation. Herpetol. Rev. 6:37-40. 19 Thomas, R.A. and F.E. Potter, Jr. 1975. Species status account -- Bufo houstonensis Sanders, 1953. In: Potter, F.E.,Jr., Special report, Houston toad study., Texas Parks and Wildlife Dept. 15 pp. References - 1 (DRAFT) - References Species TOAD, HOUSTON Species Id ESIS202001 Date 14 MAR 96 20 Hillis, D.M. and R.F. Martin. Undated Pers. Comm. in (01). 21 Quinn, H. 1983. Preliminary report of the Houston toad release program from 1 January to 1 June 1983. 3 pp. Files of USFWS, Albuquerque, NM. 22 Voris, H.K. and J.P. Bacon, Jr. 1966. Differential predation on tadpoles. Copeia 1966:594-598. 23 Blair, A.P. 1957. Amphibians. Pp. 211-271. In: Vertebrates of the United States, 1st ed. by W.F. Blair, A.P. Blair, P. Brodkorb, F.R. Cagle, and G.A. Moore. McGraw-Hill, NY. 819 pp. 24 Harwood, P.D. 1932. The helminth parasite in the Amphibia and Reptilia of Texas and vicinity. Proc. U.S. Nat. Mus. No. 2940, 81(17):22-25. ***** REFERENCES FOR N-OCCURRENCE NARRATIVE ONLY ***** 01 U.S. Fish and Wildlife Service. 1984. Houston Toad Recovery Plan. U.S. Fish and Wildl. Serv., Albuquerque, NM 72 pp. 02 McClure, W.L. 1976. Pers. Comm. rept. in (01). 03 Dixon, J.R. 1983. Survey of the Houston toad at the Caldwell, Texas site. Final Rept. to: USFWS, Albuquerque, NM. 3 pp. 04 Sanders, O. 1953. A New species of toad, with a discussion of morphology of the Bufonid skull. Herpetologica 9:25-47. 05 Blair, W.F. 1956. Call difference as an isolation mechanism in southwestern toads (Genus Bufo). Tex. J. Sci. 8:87-106. 06 Brown, L.E. 1971. Natural hybridization and trend toward extinction in some relict Texas toad populations. Southwest Nat. 16:185-199. 07 Guttman, S.I. 1969. Blood protein variation in the Bufo americanus species of toads. Copeia 1969:243-249. 08 Quinn, H. 1983. Preliminary report of the Houston toad release program from 1 January to 1 June 1983. 3 pp. Files of USFWS, Albuquerque, NM. References - 2