(DRAFT) - Taxonomy
                                Species COQUI, GOLDEN
                                Species Id ESIS204001
                                   Date 14 MAR 96



TAXONOMY

NAME - COQUI, GOLDEN OTHER COMMON NAMES - COQUI and GOLDEN; COQUI DE ORO; COQUI AUREO ELEMENT CODE - CATEGORY - Amphibians PHYLUM AND SUBPHYLUM - CHORDATA, CLASS AND SUBCLASS - AMPHIBIA, ORDER AND SUBORDER - ANURA, FAMILY AND SUBFAMILY - LEPTODACTYLIDAE, GENUS AND SUBGENUS - ELEUTHERODACTYLUS, SPECIES AND SSP - JASPERI, SCIENTIFIC NAME - ELEUTHERODACTYLUS JASPERI AUTHORITY - TAXONOMY REFERENCES - COMMENTS ON TAXONOMY - Golden Coqui Eleutherodactylus jasperi Drewry and Jones, 1976 KINGDOM: Animal GROUP: Amphibian PHYLUM: Chordata CLASS: Amphibia ORDER: Anura FAMILY: Leptodactylidae The Golden Coqui (Eleutherodactylus jasperi) is an arboreal frog easily distinguishable from other Puerto Rican Eleutherodactylids by its unique appearance and unusual reproductive biology. Small in size (16.8-22.4 mm snout-vent length) the adults are sexually monomorphic. Dorsal surfaces of head, body and limbs are smooth and lack pigmented markings. Skin color ranges from olive to pale yellow underlain by a translucent golden cast. The ventral skin of the abdomen is transparent, revealing the silver-white peritoneum, gonads and heart. Subdermal glands on dorsal surfaces of hind legs, trunk and parotoid area are usually visible as indistinct lighter patches. Both hands and feet have vestigial webbing between digits. Adults lack prevomerine teeth. Oviducal young have a small well keratinized, bifurcated egg tooth that sloughs off at about the time of birth. Fertilized internally (10,11), females carry developing eggs in a modified oviduct. Reproduction is ovoviviparous and the development Taxonomy - 1 (DRAFT) - Taxonomy Species COQUI, GOLDEN Species Id ESIS204001 Date 14 MAR 96 of young is direct (young are fully metamorphosed at hatching and subsequent birth). Young closely resemble adults in coloration and morphology (12). The reproductive character of the golden coqui initially suggested the identification of a new genus, as with the African toad (Nectophrynoides vivipara) which is similarly ovoviviparous (08). The golden coqui is left in the present generic boundaries due to early evidence of morphological and chromosomal affinities to other Puerto Rican Eleutherodactylus (07,02). The species was named for Dr. Jasper J. Loftus-Hills of New Victoria, Australia, who helped to collect the first specimen, but died in a tragic accident a few months later. The following specimens are available for species identification (04): Holotype - Field Museum of Natural History 196846, an adult male from Sierra de Cayey, 6 km SE Cayey, Puerto Rico. Collected while calling 22 May 1973 by G.E. Drewry, K.L. Jones, J.J. Loftus-Hills and J.R. Clark. Paratypes - 16 adult specimens were examined. The 16 paratypes, and the institutions housing them are: The American Museum of Natural History - AMNH 90247-49; The Field Museum of Natural History - CNHM 196944-45; The Museum of Comparative Zoology, Harvard Univ. - MCZ 88388-90; The National Museum of Natural History - USNM 198056-58; The Los Angeles County Museum - LACM 114386; The Museum of Southwestern Biology, Univ. of NM - UNM 27955. Taxonomy - 2
                                  (DRAFT) - Status
                                Species COQUI, GOLDEN
                                Species Id ESIS204001
                                   Date 14 MAR 96



STATUS

Coded Status Puerto Rico; State Listed T: Federal Threatened Commercial Non-consumptive recreational Ceremonial/Cultural Medicinal Ornamental COMMENTS ON STATUS - U.S. STATUSES AND LAWS: The golden coqui (Eleutherodactylus jasperi) has been designated a Threatened species pursuant to the Endangered Species Act of 1973 (50 CFR 17.11; P.L. 93-205, 87 Stat. 884; 16 U.S.C. 1531-1540), as amended. The species has this status wherever found including the Commonwealth of Puerto Rico. Critical Habitat has been designated in Puerto Rico (50 CFR 17.95(d)). Three areas have been designated as Critical Habitat: Cerro Avispa - elevations above 700 meters on the south and southeastern slope of the mountain, Monte el Gato - entire summit above 700 meters, and Sierra de Cayey at elevations above 700 meters. No land management recommendations are currently in effect. This species is protected by the Lacey Act (P.L. 97-79, as amended; 16 U.S.C. 3371 et seq.) which makes it unlawful to import, export, transport, sell, receive, acquire, or purchase any wild animal (alive or dead including parts, products, eggs, or offspring): (1) in interstate or foreign commerce if taken, possessed, transported or sold in violation of any State law or regulation; or (2) if taken or possessed in violation of any U.S. law, treaty, or regulation or in violation of Indian tribal law. It is also unlawful to possess any wild animal (alive or dead including parts, products, eggs, and offspring) within the U.S. territorial or special maritime jurisdiction (as defined in 18 U.S.C. 7) that is taken, possessed, transported, or sold in violation of any State law or regulation, foreign law, or Indian tribal law. RESPONSIBLE FEDERAL AGENCIES: USFWS -Responsible for the management/recovery, listing, and law enforcement/protection of this species. All Federal agencies have responsibility to ensure that any action authorized, funded, or carried out by that agency is not likely to jeopardize the continued existence of the species or result in the destruction or adverse modification of Critical Habitat (50 CFR 402), and to utilize their authorities to carry out programs for the conservation of the species. Status - 1 (DRAFT) - Status Species COQUI, GOLDEN Species Id ESIS204001 Date 14 MAR 96 STATE STATUSES AND LAWS: STATE: Commonwealth of Puerto Rico DESIGNATED STATUS: Endangered with Critical Habitat ADMINISTRATIVE AGENCY: Department of Natural Resources, Commonwealth of Puerto Rico. STATE STATUTES: Organic Act, Sec. 155; and Wildlife Act, Sec. 85 and 91. Regulation to Govern the Management of Threatened and Endangered Species in the Commonwealth of Puerto Rico, 1985. INTERNATIONAL STATUSES, TREATIES, AND AGREEMENTS: None. ECONOMIC STATUSES: The unique reproductive biology and photogenic coloration of the golden coqui will possibly result in the over collection of the species for scientific, educational, and display purposes. The species may also be sought for observation and photographic purposes. Similarly bright colored frogs from the tropics, Amphodus auratus from Trinidad and Atelopus varius zeteki from Panama, have suffered from over collection and are close to extinction. Historically, this species has had medicinal value in cultural ceremonies. 77/04/05:42 FR 18106/18108 - Proposed Threatened with Critical Hab. 77/11/29:42 FR 58755/ - Listed as Threatened w/ Critical Habitat 82/12/27:47 FR 42387/42388 - Five year review 87/07/07:52 FR 25523/25528 - Notice of review Status - 2
     

HABITAT ASSOCIATIONS

HABITAT - TERRESTRIAL TERRESTRIAL LAND USE - Residential Transportation, communications, and Util Cropland and Pasture Mixed Forest Land Bare Exposed Rock COMMENTS ON HABITAT ASSOCIATIONS - The golden coqui is found in a small restricted range south of Cayey, Puerto Rico. The life zone is characterized as a subtropical moist forest zone, receiving 1100 to 2200 mm of rainfall annually with a mean biotemperature between 18 and 24 degrees C. Most of available moisture, particularly during the dry season, comes from heavy dew condensation produced at night (09,04,07). Large areas of land have been deforested to accommodate cultivation of crops and pasture land. Scattered areas of remnant zonal associated vegetation are characterized by trees up to 20 meters. Many of the woody species are deciduous during the dry season and epiphytes are common, but seldom completely cover branches and trunks. Natural and improved pastures are common in this zone today (01,05). Within the range of the golden coqui, there is a great demand for high elevation land because of moderate rainfall and temperatures. These conditions have encouraged large scale modification of summit areas for residential development and mass cut-slash and burn deforestation of natural vegetation for agricultural and pasture purposes (05). With the exception of a highway right-of-way, most of the known habitat of the golden coqui is privately owned. The critical factor that appears to determine the distribution of this species within its range is the presence of large bromeliad clusters. Bromeliads inhabited by golden coquis are found on faces of rock cliffs, edges of short grass pastures, low-canopy forests (1-5 meters) and xeric scrub, isolated large trees, and margins of forest. Bromeliads utilized include Vriesia, Hoenbergia, Guzmania, and Tilanzia. Colonization of bromeliad clusters seems to be independent of the bromeliads' substrate (04,07,03). No information is available describing further specific habitat associations. Drewry and Clark (04) have postulated that the distribution limits of the golden coqui are due not to elevation but to a zone of aridity (09,04) that acts as a barrier to dispersion. This zone is formed by prevailing easterly winds that prevent orographic rain cloud formation. At the edge of this zone, wind velocities are low and heavy dew is produced every night, favoring the growth of epiphytic vegetation. Both distribution and abundance of the golden coqui within its range are greatly affected by habitat disturbance. This is attributed to the slow recolonization of bromeliads by the frogs in the areas that have been deforested for agricultural or developmental purposes. About half of the land area on Monte el Gato and Cerro Avispa consist of unrecolonized pastures and lawns. No golden coquis have been found in fairly extensive bromeliad growth at the summit of Cerro Planada and the adjacent peak to the west. These areas have long been planted in coffee and citrus - the planting probably accompanied by clearing of natural vegetation by slash and burn methods. Very little information is available describing habitat Habitat Associations - 1 associations of the golden coqui. This information is critical in order that those elements necessary for the conservation of the species can be identified. This is important not only to conserve the species within its present range, but also to explore areas for unknown populations. Habitat Associations - 2
                                (DRAFT) - Food Habits
                                Species COQUI, GOLDEN
                                Species Id ESIS204001
                                   Date 14 MAR 96



FOOD HABITS

TROPHIC LEVEL - CARNIVORE LIFESTAGE FOOD FOOD PART General Arthropods Food Habits - 1
                         (DRAFT) - Environment Associations
                                Species COQUI, GOLDEN
                                Species Id ESIS204001
                                   Date 14 MAR 96



ENVIRONMENTAL ASSOCIATIONS

G = General A = Adult LIM = Limiting RA = Resting Adult J = Juvenile FA = Feeding Adult RJ = Resting Juvenile BA = Breeding Adult FJ = Feeding Juvenile P = Pupae L = Larvae E = Egg RL = Resting Larvae FL = Feeding Larvae
LIFESTAGE ENVIRONMENTAL ASSOCIATIONS G Coastal Features: Vegetated offshore islands G Terrestrial Features: Cliffs/ledges G Terrestrial Features: Rock outcrops Environment Associations - 1
                               (DRAFT) - Life History
                                Species COQUI, GOLDEN
                                Species Id ESIS204001
                                   Date 14 MAR 96



LIFE HISTORY

FOOD HABITS: Very little information is available describing foraging behavior and type of food required by the golden coqui. It is assumed that the frog forages on small insects - particularly those insects attracted to the water in the axils of the bromeliad (02). Only a few observations have been made of this species foraging on plants away from the bromeliad. This is in contrast to observations of the foraging behaviors of the parapatric coquis, E. gryllus and E. cochranae. At night these frogs emerge to forage widely over the tree or shrub bearing the bromeliad, and on adjacent vegetation (03). Drewry, Jones and Clark (03,07,02) believe that most of the frogs' needs are met within a single bromeliad or bromeliad cluster. This is interesting in light of groups of coquis - multiple adults and juveniles of different ages - utilizing a single bromeliad cluster. Studies on food-resource partitioning of the golden coqui are needed to understand food items essential to its survival. HOME RANGE/TERRITORY: Drewry and Clark (03) and Drewry (14) describe interactions among caged individuals or of frogs experimentally transferred in the field. Adult golden coquis are strongly territorial against other adults of their own kind and other small frogs. They defend their axil fiercely against all other frogs, shoving, biting and, in the case of males, calling before each attack. No more than one adult was typically found in a small bromeliad, and no more than two of opposite sex in a large bromeliad, spaced even then as far apart as possible. In contrast the young were usually found in leaf axils near an adult, presumably their mother. Two or three size classes of young were often present. This is unlike other members of the genus Eleutherodactylus in which young normally disperse from the adult within 5 days of hatching (12,10). A "normal" group is 1 or 2 adults and 2 or more size classes of juveniles per bromeliad, but almost never more than 1 frog per axil. The group or "social" relationships of the golden coqui remain to be investigated in greater detail. PERIODICITY: The golden coqui appear to conform to primarily nocturnal cycles of behavior as is typical of the genus. In daylight, the frogs sit quitely with vent just touching the water in an axil. From this position they capture insects entering the axil or slide backward under the water if a larger intruder appears. At night they venture further out on the same leaf or to others nearby (03). Drewry (02) described the seasonal vocalization pattern as occurring throughout the spring (early March) through late fall (November and December) and typically climaxing in intensity during the early summer (June). Gravid females were collected from April to August. The immature coquis of 2 or more different size and age groups suggest that these frogs have multiple clutches each season. MIGRATORY PATTERN: Non-migratory. Life History - 1 (DRAFT) - Life History Species COQUI, GOLDEN Species Id ESIS204001 Date 14 MAR 96 COVER/SHELTER REQUIREMENTS: The golden coqui is an obligate bromeliad dweller utilizing the security of the bromeliad axils by day and the leaves as a foraging substrate at night. Colonization of bromeliad clusters seems to be independent of ground, tree, or vertical rock surface placement of the bromeliad (07). Drewry and Clark (03) rank substrates by frequency, the most frequently encountered substrates are rock cliffs and low canopy xeric scrub. REPRODUCTIVE SITE REQUIREMENTS: No specific information is available. It is presumed that since adults were rarely observed outside of a bromeliad, amplexus and birth of young occur either in the water filled bromeliad leaf axils or on the leaf itself (02). REPRODUCTIVE CHARACTERISTICS: The golden coqui is a terrestrial breeding frog. The males call from bromeliads from March through December (02). Fertilization of the females is internal (11,12) and gravid females have been collected from April to August. The eggs are heavily yolked and number from 1 to 6 (04,12). The female carries the developing eggs in a modified fold of the oviduct during the entire gestation period of approximately 1 month (04,10,12). Intraoviducal nutrition is dependent on the yolk throughout development (12). Eggs undergo direct development and hatch as tiny froglets. The young remain with an adult for an undetermined amount of time. Observation of 2 or more size classes of subadults in the same bromeliad with an adult suggests that a female may reproduce more than 1 time per year (03,12). PARENTAL CARE: No indication of parental care has been demonstrated after the hatching of immature golden coquis. Juveniles of this species less than half grown - sometimes of more than 1 age and size group - were always found in proximity to adults. This observation is in contrast to other Eleutherodactylus species in which hatchlings disperse from the adults within a few days of hatching (10,11). Lack of dispersion in juvenile golden coquis has been attributed to the semi-xeric environment rather than to parental care. POPULATION BIOLOGY: Very little demography information is available for the golden coqui. Between May 1973 and August 1974, Dr. G. Drewry estimated the following populations based on census' of vocalizations (02): Cerro Avispa - less than 10; Monte el Gato - 500-1000; Sierra de Cayey - 1000-2000. These population estimates are the only figures available. SPECIES INTERRELATIONSHIPS: Drewry and Clark found possible evidence of range exclusion between the golden coqui, and 2 parapatric species of coqui - E. gryllus and E. cochranae. Directly north and east of the outer Life History - 2 (DRAFT) - Life History Species COQUI, GOLDEN Species Id ESIS204001 Date 14 MAR 96 boundaries of the golden coqui distribution and at elevations above 700 m, E. gryllus frequents bromeliads along the edges of tall forests. E. cochranae is found from the lowlands to 700 m south and west of the golden coqui range, inhabiting litter-filled axils of bromeliads. Surveys have indicated that all 3 species of axil-dwelling Eleutherodactylids are mutually exclusive in ranges. Drewry (02,03) has found evidence of upward range extension of E. cochranae in the Sierra de Cayey area. Since this area is possibly the best habitat available (02), the spread of E. cochranae into the golden coqui range could be a significant problem in the recovery of the species. OTHER LIFE HISTORY DESCRIPTORS: None. Life History - 3
                           (DRAFT) - Management Practices
                                Species COQUI, GOLDEN
                                Species Id ESIS204001
                                   Date 14 MAR 96



MANAGEMENT PRACTICES

RESULT MANAGEMENT PRACTICE Beneficial Suppressing wildfire Beneficial Prescribed/controlled burning of habitat Beneficial Maintaining undisturbed/undeveloped areas Beneficial Restricting/regulating human use of habitats Beneficial Land Acquisition Beneficial Stocking captive-reared wild-strain animals Beneficial Transplanting wild animals Beneficial Restricting Poaching Beneficial Regulating commercial harvest levels Beneficial Controlling/Removing Native Vertebrates Adverse Collecting Existing Collecting Adverse Inherent Reproductive Characteristics Existing Inherent Reproductive Characteristics Adverse Low Gene Pool Existing Low Gene Pool Adverse Rural Residential/Industrial Areas Existing Rural Residential/Industrial Areas Adverse Highway/Railroads Existing Highway/Railroads Adverse Competition Existing Competition Adverse Grazing Existing Grazing Adverse Existing Adverse Fire Existing Fire Adverse Harvesting Existing Harvesting COMMENTS ON MANAGEMENT PRACTICES - The golden coqui (Eleutherodactylus jasperi) is very rare and restricted in range to a small area about 5 km in diameter where it inhabits dense bromeliad growth at elevations of 700 to 850 meters (04). Drewry and Jones (04) indicated that the species is threatened due to past and potential loss of habitat, its obligate bromeliad dwelling mode of existence, its presumed low reproductive rate, the potential for overcollection, and an apparent inability to disperse. Within the range of the golden coqui, there is a great demand for high elevation land because of moderate rainfall and temperatures. These conditions have encouraged large scale modification of summit areas for residential development and mass cut-slash and burn deforestation of natural vegetation for agricultural and pasture purposes (05). With the exception of highway right-of-way, most of the known habitat of the golden coqui is privately owned. Habitat destruction clearly demonstrates the need for land usage regulations within its range. Fire is believed to be one of the most important threats to the Management Practices - 1 (DRAFT) - Management Practices Species COQUI, GOLDEN Species Id ESIS204001 Date 14 MAR 96 golden coqui primarily because of the semi-xeric nature of the vegetation. In 1973 one scrub area contained golden coquis in bromeliads on the ground, in low trees and on surfaces of large boulders. The population was reexamined following a fire. The only golden coquis which survived the fire were those in bromeliads on rock surfaces (07). Fire can have a catastrophic effect on golden coqui populations, particularly where fire control has allowed a heavy build-up of fuel. Appropriate fire management methods need to be employed where practical to minimize adverse effects of fire. The unique reproductive biology and attractive coloration are likely to create a high demand for specimens for scientific, educational and display purposes. It has been suggested that without proper regulation, the fate of the golden coqui may closely parallel that of the golden frog of Trinidad (Amphodus auratus) and the Panamanian golden frog (Atelopus varius zeteki). Both frogs are extremely photogenic and have highly restricted ranges. Both species have been over collected and are close to extinction (07). Potential for gold and tungsten mining exists in the mountains between Cayey and Salinas, which could be a potential threat to the species (14). Drewry and Clark found possible evidence of range exclusion between the golden coqui, and 2 parapatric species of coqui - E. gryllus and E. cochranae (03). Directly north and east of the outer boundaries of the golden coqui distribution and at elevations above 700 m, E. gryllus frequents bromeliads along the edges of tall forests. E. cochranae is found from the lowlands to 700 m south and west of the golden coqui range, inhabiting litter-filled axils of bromeliads. Surveys have indicated that all 3 species of axil-dwelling Eleutherodactylids are mutually exclusive in ranges. Drewry (02,03) has found evidence of upward range extension of E. cochranae in the Sierra de Cayey area. Since this area is possibly the best habitat available (02), the spread of E. cochranae into the golden coqui range could be a significant problem in the recovery of the species. The current population of the golden coqui is quite restricted in its distribution and thus susceptible to habitat destruction and mass collection of specimens. As a result, with information provided by Drs. George E. Drewry and Kirkland L. Jones, the species was designated as Threatened (Federal Register 42(65):18106-18108 04/05/07), and Critical Habitat determined in the three summit areas of Cerro Avispa, Monte el Gato and Sierra de Cayey above 700 meters. APPROVED PLAN: U.S. Fish and Wildlife Service. 1984. Golden Coqui Recovery Plan. U.S. Fish and Wildlife Service, Atlanta, GA. 12 pp. The following recommendations are included in the Recovery Plan for the golden coqui: 1. Implement actions necessary to protect Critical Habitat such as land useage regulations, controlling development (urban and agricultural), land purchase, etc. 2. Establish specific habitat associations and conduct a status survey Management Practices - 2 (DRAFT) - Management Practices Species COQUI, GOLDEN Species Id ESIS204001 Date 14 MAR 96 in each of the three Critical Habitat areas. 3. Survey similar habitats outside of the Critical Habitat area for unknown populations, and evaluate the feasibility of establishing other populations by transplantation of individuals. 4. Explore potential benefits of captive propagation as a fallback plan or for stock to populate new areas distant from the current sites. 5. Investigate potential of competitive exclusion of the golden coqui from E. cochranae and implement necessary preventative/corrective measures. 6. Develop a long term fire management plan to be employed where practical to minimize adverse effects of fire. Fire can have a catastrophic effect on golden coqui populations, particularly where fire control has allowed a heavy build-up of fuel. Appropriate fire management methods (such as prescribed burning or fire control when conditions and fuel quanity are undesirable) need to be employed where practical to minimize adverse effects of fire. Recovery objectives have been recommended to be defined as 3 populations which are stable or expanding, with each population containing at least 1000 adults, and long term essential habitat protection through appropriate means. Although it was not included in the original Recovery Plan, Drewry (14) recommends an extensive formal survey to obtain current status. This would be accomplished through examining all known collection localities and other areas ecologically similar to the collection localities. In view of the recent failure to locate individuals of this species, plans and immediate steps to institute captive propagation if/when individuals are found is also recommended. The species has been maintained in captivity for periods of several months before. The restricting of poaching for individual or commercial purposes should be restricted in view of the rarity of this species. Management Practices - 3
                                   (DRAFT) - References
                                  Species COQUI, GOLDEN
                                  Species Id ESIS204001
                                      Date 14 MAR 96



     

References

***** REFERENCES FOR ALL NARRATIVES EXCEPT N-OCCURRENCE ***** 01 Birdsey, R.A. and P.L. Weaver. 1982. The Forest Resources of Puerto Rico. USDA For. Serv. Res. Bull. No. 85:59 pp. Inst. Trop. For. Rio Piedras, PR. 02 Drewry, Dr. George E. August 26, 1983. Personal communication to Tammie Nakamura. U.S. Dept. of Interior, USFWS, OES Washington, D.C. 20240. 03 Drewry, G.E. and J.R. Clark. 1977. Microhabitat, competition, climatic relationships and distribution. Part III. U.S. Dept. of Interior, USFWS, OES Washington, D.C. 20240. 04 Drewry, G.E. and K.L. Jones. 1976. A new ovoviviparous frog, Eleutherodactylus jasperi (Amphibia, Anura, Leptodactylidae) from Puerto Rico. J. Herpet. 10(3):161-165. 05 Ewel, J.J. and J.L. Whitmore. 1973. The ecological life zones of Puerto Rico and the U.S. Virgin Islands. USDA For. Serv. Res. Paper ITF-18. 72 pp. Inst. Trop. For., Rio Piedras, PR. 06 Goin, C.J., O.B. Goin, and G.R. Zug. 1978. Introduction to Herpetology. Freeman, San Francisco, CA. 99 pp. 07 Jones, K.L. 1975. Proposed Endangered species account for the Golden Coqui. Personal communication to Dr. James D. Williams. Dept. of Biology, Southern Methodist Univ., Dallas, TX 752704. 08 Noble, G.K. 1926. An analysis of the remarkable case of distribution among the Ambhibia - with descriptions of new genera. Amer. Mus. Novitates. 212:1-24. 09 Rivero, J.A. 1963. Notes on the distribution of some Puerto Rican frogs, with a discussion of the possible origin of Eleutherodactylus locustus. Carib. J. Sci. 3(2):81-85. 10 Rivero, J.A. 1978. Los anfibios y reptiles de Puerto Rico. Editorial Universitaria. Universidad de Puerto Rico. Mayaguez, PR. 22 pp. 11 Townsend, D.S., M.M. Stewart, F.H. Pough and P.F. Brussard. 1981. Internal fertilization in an oviparous frog. Science 212:469-471. 12 Wake, M.H. 1978. The reproductive biology of Eleutherodactylus jasperi (Amphibia, Anura, Leptodactylidae) with comments on the evolution of live-bearing systems. J. Herpet. 12(2):121-133. 13 Anon. 1980. Checklist of Reptiles and Amphibians. Assoc. of Systematic Collectors, Lawrence, KS. 14 Drewry, G.E. 1986. Personal communication. Biologist, U.S. Fish and Wildlife Service, Washington, D.C. 20240. ***** REFERENCES FOR N-OCCURRENCE NARRATIVE ONLY ***** 01 Drewry, Dr. George E. August 26, 1983. Personal communication to Tammie Nakamura. U.S. Department of Interior, USFWS, OES. Washington, D.C. 20240. 02 Drewry, G.E. and J.R. Clark. 1977. Microhabitat, competition, climatic relationships and distribution. Part III. U.S. Department of Interior, USFWS, OES. Washington, D.C. 20240. 03 Drewry, G.E. and K.L. Jones. 1976. A new ovoviviparous frog, References - 1 (DRAFT) - References Species COQUI, GOLDEN Species Id ESIS204001 Date 14 MAR 96 Eleutherodactylus jasperi (Amphibia, Anura, Leptodactylidae) from Puerto Rico. J. Herpet. 10(3):161-165. 04 Jones, K.L. 1975. Proposed Endangered species account for the Golden Coqui. Personal communication to Dr. James D. Williams. Department of Biology, Southern Methodist University, Dallas, TX 752704. 05 Drewry, G.E. 1986. Personal communication. Biologist, U.S. Fish and Wildlife Service, Washington, D.C. 20240. References - 2