(DRAFT) - Taxonomy
                       Species PUPFISH, AMARGOSA, ASH MEADOWS
                                Species Id ESIS251003
                                   Date 14 MAR 96



TAXONOMY

NAME - PUPFISH, AMARGOSA, ASH MEADOWS OTHER COMMON NAMES - PUPFISH, AMARGOSA, ASH MEADOWS; PUPFISH, ASH MEADOWS; PUPFISH, AMARGOSA, BIG SPRING; PUPFISH, NEVADA;PUPFISH and AMARGOSA ELEMENT CODE - CATEGORY - Fish PHYLUM AND SUBPHYLUM - CHORDATA, CLASS AND SUBCLASS - OSTEICHTHYES, ORDER AND SUBORDER - ATHERINIFORMES, FAMILY AND SUBFAMILY - CYPRINODONTIDAE, GENUS AND SUBGENUS - CYPRINODON, SPECIES AND SSP - NEVADENSIS, MIONECTES SCIENTIFIC NAME - CYPRINODON NEVADENSIS MIONECTES AUTHORITY - TAXONOMY REFERENCES - COMMENTS ON TAXONOMY - Ash Meadows Amargosa Pupfish Cyprinodon nevadensis mionectes Miller, 1948 KINGDOM: Animal GROUP: Fish PHYLUM: Chordata CLASS: Osteichthyes ORDER: Atheriniformes FAMILY: Cyprinodontidae Cyprinodon nevadensis was first described from Saratoga Springs, Death Valley, California in 1889 (05). The range of morphological and meristic variation within the various populations of C. nevadensis was reviewed in 1943 (09), but it was not until 1948 that subspecies of C. nevadensis, including C. n. mionectes, were formally described (10). Cyprinodon nevadensis is the most morphologically variable species of pupfish, but can be defined by the following combination of characters: Scale surface deeply reticulate, ciculi without obvious spine-like projections; scales large, usually 25 to 26 in lateral series; central cusp of tricuspid teeth narrower than outer cusps; and breeding color of males deep blue and without yellow color (09,11). In his description of C. n. mionectes, Robert Miller distinguished this subspecies from others in that the former has scale and finray counts lower than average for the species; a reduced body size; a short, deep, and slab-sided body with a greatly arched and Taxonomy - 1 (DRAFT) - Taxonomy Species PUPFISH, AMARGOSA, ASH MEADOWS Species Id ESIS251003 Date 14 MAR 96 compressed predorsal profile; and a very long head and opercle (10). Of the low scale counts, the number around the caudal peduncle was particularly diagnostic (mean= 13 - 15 vs. 15.4 - 16 for other subspecies) (10). Variation does exist among the various populations of C. n. mionectes. This variation has been described and the various populations have been termed "races" (10). Common name synonyms include: Ash Meadows pupfish, Big Spring Amargosa pupfish, Nevada pupfish, Ash Meadows Amargosa pupfish, and Amargosa pupfish. Type specimens of Cyprinodon nevadensis mionectes are deposited at the University of Michigan Museum of Zoology. Photographs of the type specimens are included in the original description (10) and some excellent drawings and color photographs of C. nevadensis and other species of pupfish can be found in Soltz and Naiman 1978 (15). Taxonomy - 2
                                  (DRAFT) - Status
                       Species PUPFISH, AMARGOSA, ASH MEADOWS
                                Species Id ESIS251003
                                   Date 14 MAR 96



STATUS

Coded Status E: Federal Endangered Non-consumptive recreational COMMENTS ON STATUS - U.S. STATUSES AND LAWS: The Ash Meadows Amargosa pupfish (Cyprinodon nevadensis mionectes) has been designated an Endangered species pursuant to the Endangered Species Act of 1973 (50 CFR 17.11; P.L. 93-205, 87 Stat. 884; 16 U.S.C. 1531-1540), as amended. The subspecies has this status wherever found including the State of Nevada. Critical Habitat has been designated in Nye County, NV (50 CFR 17.95(e)) in portions of: T17S R50E, Sec. 9, 15, 22, and 23; T18S R50E, Sec. 3 and 11; and T18S R51E, Sec. 7, 18, and 19. This subspecies is protected by the Lacey Act (P.L. 97-79, as amended; 16 U.S.C. 3371 et seq.) which makes it unlawful to import, export, transport, sell, receive, acquire, or purchase any wild animal (alive or dead including parts, products, eggs, or offspring): (1) in interstate or foreign commerce if taken, possessed, transported or sold in violation of any State law or regulation; or (2) if taken or possessed in violation of any U.S. law, treaty, or regulation or in violation of Indian tribal law. It is also unlawful to possess any wild animal (alive or dead including parts, products, eggs, and offspring) within the U.S. territorial or special maritime jurisdiction (as defined in 18 U.S.C. 7) that is taken, possessed, transported, or sold in violation of any State law or regulation, foreign law, or Indian tribal law. RESPONSIBLE FEDERAL AGENCIES: USFWS -Responsible for the management/recovery, listing, and law enforcement/protection of this species. BLM -Responsible for the law enforcement/protection of this species with applicable State and Federal laws on public land under their control (43 CFR 4140). Also responsible for management/recovery on Bureau of Land Management lands. All Federal agencies have responsibility to ensure that any action authorized, funded, or carried out by that agency is not likely to jeopardize the continued existence of the species or result in the destruction or adverse modification of Critical Habitat (50 CFR 402), and to utilize their authorities to carry out programs for the conservation of the species. Status - 1 (DRAFT) - Status Species PUPFISH, AMARGOSA, ASH MEADOWS Species Id ESIS251003 Date 14 MAR 96 STATE STATUSES AND LAWS: STATE: Nevada DESIGNATED STATUS: Protected, Endangered ADMINISTRATIVE AGENCY: Nevada Department of Wildlife STATE STATUTE: Nevada Admin. Code, 503.015 to 503.080. INTERNATIONAL STATUSES, TREATIES, AND AGREEMENTS: Cyprinodon nevadensis mionectes is listed as Vulnerable in the IUCN Red Data Book, Vol. 4 (1977). ECONOMIC STATUSES: As a species within the Ash Meadows National Wildlife Refuge, occupying a unique and fragile habitat, the Amargosa pupfish has educational and interpretive values and attracts significant attention for these purposes. 82/05/10:47 FR 19995/ - Emergency rule, listed Endangered w/ CH 83/01/05:48 FR 00608/00616 - Emergency rule, listed Endangered w/ CH 83/01/05:48 FR 00617/00625 - Proposed rule, list as Endangered w/ CH 83/09/02:48 FR 40178/40186 - Final rule, listed as Endangered w/ CH Status - 2
     

HABITAT ASSOCIATIONS

HABITAT - AQUATIC INLAND AQUATIC LAND USE - Residential Herbaceous Rangeland Shrub and Brush Rangeland Mixed Rangeland Streams and Canals Reservoirs Nonforested Wetland NATIONAL WETLAND INVENTORY CODES NWI NWICLS NWIMOD NWISPEC Riverine, intermittent UB4 Riverine, intermittent UB3 Riverine, intermittent UB2 Riverine, intermittent UB1 Riverine, intermittent SB4 Riverine, intermittent SB3 Riverine, intermittent SB2 4SB Riverine, intermittent RB2 Riverine, intermittent RB1 Riverine, intermittent OW0 Riverine, intermittent AB3 Riverine, intermittent AB2 Riverine, intermittent AB1 Riverine, upper perennial UB4 Riverine, upper perennial UB3 Riverine, upper perennial UB2 Riverine, upper perennial UB1 3SB Riverine, upper perennial SB3 Riverine, upper perennial SB2 Riverine, upper perennial SB1 Riverine, upper perennial RB2 Riverine, upper perennial RB1 Riverine, upper perennial OW0 Riverine, upper perennial AB3 Riverine, upper perennial AB2 Riverine, upper perennial AB1 Riverine, lower perennial UB4 2UB Riverine, lower perennial UB2 Riverine, lower perennial UB1 Riverine, lower perennial SB4 Riverine, lower perennial SB3 Riverine, lower perennial SB2 Riverine, lower perennial SB1 Riverine, lower perennial RB2 Riverine, lower perennial RB1 Riverine, lower perennial OW0 Riverine, lower perennial AB3 2AB Riverine, lower perennial AB1 Habitat Associations - 1 NWI NWICLS NWIMOD NWISPEC Lacustrine, limnetic UB4 Lacustrine, limnetic UB3 Lacustrine, limnetic UB2 Lacustrine, limnetic UB1 Lacustrine, limnetic OW0 Palustrine EM2 Palustrine EM1 Palustrine AB3 Palustrine AB2 Palustrine AB1 COMMENTS ON HABITAT ASSOCIATIONS - Ash Meadows is a large oasis in southwestern Nevada. Its nearly 50 seeps and springs discharge about 17,000 acre-feet of water annually (33,35). This water formerly flowed into an extensive marsh which was drained in the mid 1960's. Thunderstorms occasionally caused floodwaters to discharge from Ash Meadows into the Amargosa River which terminates in the floor of Death Valley. Ash Meadows is home to some 30 species of endemic plants, insects, snails, other invertebrates and fish. Human development outside of Ash Meadows NWR consists primarily of small scattered residences with which subsistence gardens, small orchards or agricultural fields may be associated. During the early 1970's a large-scale farm began operating in Ash Meadows. Development of the large farm involved extensive removal of natural vegetation, land leveling, construction of irrigation wells, ditches and fences and other activities necessary for commercial farming (34,36). Primary crops grown included alfalfa, cotton and sorghum. Cattle were grazed on developed and irrigated pasture. A U.S. Supreme Court decision limited groundwater pumping by the large farm in 1976 (37). The land was sold to a developer who continued farming on a smaller scale while preparing the land for subdivision development. The 12,663 acre subdivision, after extensive efforts by conservationists, was purchased by The Nature Conservancy in 1984 and then resold to the U.S. Fish and Wildlife Service (USFWS). The USFWS plans to restore the area for use as a Wildlife Refuge (31). Currently there are no agricultural uses associated with Ash Meadows NWR. Natural vegetation on the nearly level terrain near the springs is dominated by groves of ash trees (Fraxinus velutina var. coriacea) and screwbean (Prosopis pubescens) in association with a large scrub (Baccharis emoryi). Sand dunes in the area are donimated by mesquite (Prosopis glandulosa var torreyana). Shadscale (Atriplex confertifolia) and Haplopappus (Haplopappus acradenius) dominate areas away from direct influence of the spring waters. Large areas of seasonally wet, salt-encrusted soils are covered with saltgrass (Distichlis spicata). Greasewood (Larrea sp.) dominates the better drained soils on the surrounding slopes. The Ash Meadows Amargosa pupfish (Cyprinodon nevadensis mionectes) and the Ash Meadows speckled dace (Rhinichthys osculus nevadensis) occurred a large number of the springs and outflow streams and remnants of the large marsh at lowest elevations in Ash Meadows. The Warm Springs pupfish (Cyprinodon nevadensis pectoralis) lives in a few springs at slightly higher elevation. Warm Springs pupfish habitats have been altered relatively little, because they were never included in the large commercial farm or in the area targeted for subdivision development. A private residence, road, culverts and fencing are obvious features that today alter the natural water courses. Some grazing has occurred in the area, but BLM removed many feral horses from the area. Habitat Associations - 2 The Ash Meadows Amargosa pupfish occurs in a variety of low elevation (670-710 m) spring systems (03,15). Although the abundance of the pupfish varies within each spring system, it occupies headwater spring pools as well as outlfow creeks 2 km or more distant, marshes connected to spring systems, and ephemeral pools formed by spring waters (06,10,14,17). The pupfish also occurs in irrigation ditches and canals that utilize the spring flows for irrigation. Most data indicate that water temperatures of the springs are constant. Springs inhabited by the Ash Meadows Amargosa pupfish range from about 27 to 31 degrees C (10). Spring outflows and marshes presently or formerly occupied by the Ash Meadows Amargosa pupfish are thermally variable (10,26). Similar habitats occupied by closely related subspecies have more complete thermal information (24,25,26, 27,21,20). C. n. nevadensis occurs in Saratoga Springs and its associated marsh where temperatures range between 2 and 44 degrees C (20). These temperatures approach or exceed the thermal limits that have been established for survival of the Ash Meadows Amargosa pupfish (21,22,23,30). Critical thermal maximum and minimum temperatures for the subspecies when reared at 29 degrees C is 41.7 and 2.8 degrees C, respectively (22,23). The upper lethal temperature for fish living at 26.5 degrees C was 41.2 degrees C (21). Lower lethal temperatures for fish acclimatized to 31.4 degrees C was 4-5 degrees C (21). Lethal temperatures can be shifted slightly up or down by acclimatization or acclimation (23). Salinity in spring habitats occupied by the Ash Meadows Amargosa pupfish is very low (28,30). Marsh habitats in the Ash Meadows area would doubtlessly show an increase in total dissolved solids. Such increase would, however, be unlikely to present serious osmotic challenges to the pupfish since closely related species have been shown capable of surviving exposure to extremely high salinities (29, 30). In summary, the Ash Meadows Amargosa pupfish occupies a variety of spring, stream and marsh habitats in Ash Meadows, which are currently affected by small scale farm plots. Pupfish numbers presently are greatest, perhaps, in calm, clear, spring pools; although they also occur in rather rapidly-flowing creeks (17). Dense populations probably formerly occurred in the extensive Carson Slough. Marsh habitats are densely populated by other subspecies of pupfishes nearby at Tecopa (24) and at Saratoga Springs (20). Most habitats possess clear water (10). Substrates vary from soft, easily-roiled silt in spring pools, to fine gravels in outflow creeks (10,17). Aquatic vegetation may be present or absent in areas where pupfish occur. Juveniles occur in shallower water than adults and may be more dependent upon the presence of cover (11,15). Physical and chemical characteristics of the environment may be extremely constant or highly variable. Habitat Associations - 3
                                (DRAFT) - Food Habits
                       Species PUPFISH, AMARGOSA, ASH MEADOWS
                                Species Id ESIS251003
                                   Date 14 MAR 96



FOOD HABITS

TROPHIC LEVEL - OMNIVORE LIFESTAGE FOOD FOOD PART General Floating aquat. plant General Vascular Plants-Emergent Nonwoody General Vascular Plants-Submerged Nonwoody General Evergreen Shrubs-Flowers/Fruit/Seed General Carrion General Detritus General Diatoms General Algae General Zooplankton General Arthropods General Crustaceans General Molluscs Food Habits - 1
                         (DRAFT) - Environment Associations
                       Species PUPFISH, AMARGOSA, ASH MEADOWS
                                Species Id ESIS251003
                                   Date 14 MAR 96



ENVIRONMENTAL ASSOCIATIONS

G = General A = Adult LIM = Limiting RA = Resting Adult J = Juvenile FA = Feeding Adult RJ = Resting Juvenile BA = Breeding Adult FJ = Feeding Juvenile P = Pupae L = Larvae E = Egg RL = Resting Larvae FL = Feeding Larvae
LIFESTAGE ENVIRONMENTAL ASSOCIATIONS G G G Aquatic Features: Pool areas G Aquatic Features: Springs [flowing] G G Availability of fence rows: roadside ditches and grassy Environment Associations - 1
                               (DRAFT) - Life History
                       Species PUPFISH, AMARGOSA, ASH MEADOWS
                                Species Id ESIS251003
                                   Date 14 MAR 96



LIFE HISTORY

FOOD HABITS: The primary food of Amargosa pupfish, Cyprinodon nevadensis, appears to be plant material, especially algae (11). Their intestines are long and convoluted, characteristic of herbivores. Small invertebrates also are consumed, and may constitute a substantial portion of the diet in certain seasons (11,12). The particular food habits of C. n. mionectes have not been studied, however, other subspecies of C. nevadensis have been examined. The diet of C. n. amargosae, which inhabits the Amargosa River, consists primarily of detritus and diatoms (12). Smaller amounts of ostracods and chironomid larvae were also consumed (12). The diet of C. n. nevadensis of Saratoga Springs was more diverse. Detritus, algae, Ceratophyllum demersum, Tamarix flower buds, hydrobiid snails, amphipods and ostracods were primary foods (12,25). Although the long intestine would seem to indicate that C. n. mionectes has an herbivorous feeding habit, omnivory is indicated. In spring habitats with a more diverse invertebrate fauna, many molluscs, microcrustaceans and insect larvae are consumed. Terrestrial insects which fall into the water may also be consumed. HOME RANGE/TERRITORY: Mature male Ash Meadows Amargosa pupfish are territorial during the spawning season and actively defend their territories from other males (07,14). A consort pair breeding system has also been observed in this subspecies (07). Males are territorial throughout the breeding season, which can be nearly year-round in the warm, constant-temperature springs of Ash Meadows. Territoriality and consort-pair spawning apparently breaks down to be replaced by group spawning at high population densities (30). PERIODICITY: Activity cycles are largely unknown. Pupfish are usually quite active throughout the year in the warm springs (11,14). As distance from the constant-temperature springs increases, water temperature is more variable and some seasonal peaks in activity could be expected (02,07). Activity is probably severely curtailed at temperatures below 10 degrees C or above 38 degrees C (20,25). Daily periodicity tends toward crepuscular activity but is frequently controlled by temperature in the more variable environments in the closely related C. n. nevadensis (02,20,25). MIGRATION PATTERNS: The Ash Meadows Amargosa pupfish is non-migratory. No literature indicates migratory behavior for pupfish. COVER/SHELTER REQUIREMENTS: Little information is available concerning the specific habitat requirements for the Ash Meadows Amargosa pupfish. Spawning habitat usually consists of open water with soft silt or sand substrate (11, 14). Juvenile fish are more often found along the margins of spring pools and creeks where aquatic vegetation is more abundant (32). Life History - 1 (DRAFT) - Life History Species PUPFISH, AMARGOSA, ASH MEADOWS Species Id ESIS251003 Date 14 MAR 96 For the closely related C. n. nevadensis, living in a constant temperature spring pool there is a tendency for smaller individuals to prefer heavily vegetated, near-surface areas while larger fish prefer vegetated areas near the bottom (25). REPRODUCTIVE SITE REQUIREMENTS: During the breeding season, males actively defend territories. The spawning substrate is generally a loose mixture of silt, sand and detritus (15). In most spring systems, spawning habitat is limited and there is an excess of mature males (14,15). Thus, territories are often closely packed. No nests are constructed. Instead, females deposit one to two eggs at a time, which are immediately fertilized by the attending male. REPRODUCTIVE CHARACTERISTICS: In the constant-temperature environment, spawning usually extends from February to September (15). But, in some springs, spawning occurs throughout the year (15). Growth is rapid in warm springs and sexual maturity can be reached at four to six weeks (10), or when fish are 20 mm standard length (14,30). Warm springs may produce several generations per year (11). Cooler spring outflows, creeks, and marsh habitats produce fewer generations of pupfish per year (14). Females maturing more rapidly tend to produce more eggs than those that mature more slowly, and, while the number of eggs laid per day is highly variable, a 32 mm (SL) female can be expected to produce about 25 eggs (30). Ash Meadows Amargosa pupfish exhibit considerable behavioral plasticity (14,30). Consort pairing tends to dominate early in the year at low population densities, males begin territorial behavior as sex ratios approach 5:2 (male:female). At higher population densities territoriality begins to break down and the pupfish form loose aggregations with only occasional consort pair spawning (30). PARENTAL CARE: No parental care is known, although territorial males provide some protection to eggs within their territorial boundaries. POPULATION BIOLOGY: Despite large numbers of exotic species, large populations of Ash Meadows Amargosa pupfish persist at several springs. Jack Rabbit and Big Springs harbor large, viable poulations of pupfish in spring pools and outflows (17). Limiting factors have not been studies in detail but probably relate primarily to the presence of exotic competitors and predators. Predation occurs from exotic bullfrogs and crayfish as well as the exotic fishes (10,15). In more natural systems where exotic species are less of a problem, a lack of suitable spawning sites may be a primary limiting factor (14,15,07). SPECIES INTERRELATIONSHIPS: Because of predation and/or competition pressures, reductions in the population of mosquitofish (Gambusia affinis) and sailfin mollies (Poecilia latipinna) would very likely result in increased populations of pupfish (17). Also, reduced populations of crayfish and bullfrogs Life History - 2 (DRAFT) - Life History Species PUPFISH, AMARGOSA, ASH MEADOWS Species Id ESIS251003 Date 14 MAR 96 would benefit the pupfish (17). The Ash Meadows Amargosa pupfish also occupies habitats of the endangered Ash Meadows speckled dace (Rhinichthys osculus nevadensis) and the now extinct Ash Meadows poolfish (Empetrichthys merriami) (03, 17). OTHER LIFE HISTORY DESCRIPTORS: None. Life History - 3
                           (DRAFT) - Management Practices
                       Species PUPFISH, AMARGOSA, ASH MEADOWS
                                Species Id ESIS251003
                                   Date 14 MAR 96



MANAGEMENT PRACTICES

RESULT MANAGEMENT PRACTICE Beneficial Controlling/Restricting Off-Road Vehicles Beneficial Restricting/regulating human disturbance of populations Beneficial Maintaining/Controlling Water Flow Beneficial Developing/maintaining/protecting wetlands Beneficial Controlling water levels Beneficial Maintaining undisturbed/undeveloped areas Beneficial Land Acquisition Beneficial Water Right Acquisition Beneficial Reforestation Beneficial Controlling/Removing Nonnative Vegetation Beneficial Controlling/Removing Invertebrates Beneficial Controlling/Removing Exotic Vertebrates Adverse Disease Existing Disease Adverse Parasites Existing Parasites Adverse Predation Existing Predation Adverse Rural Residential/Industrial Areas Existing Rural Residential/Industrial Areas Adverse Draining wetlands, marshes, ponds, lakes Existing Draining wetlands, marshes, ponds, lakes Adverse Water Level Fluctuation Existing Water Level Fluctuation Adverse Groundwater drawdown Existing Groundwater drawdown Adverse Irrigating Existing Irrigating Adverse Reservoirs Existing Reservoirs Adverse Dredging Existing Dredging Adverse Developing/maintaining stream bank vegetation Existing Developing/maintaining stream bank vegetation Adverse Competition Existing Competition Adverse Exotic/Feral/Introducted Species Existing Exotic/Feral/Introducted Species Adverse Existing Adverse Vegetation Composition Changes Existing Vegetation Composition Changes COMMENTS ON MANAGEMENT PRACTICES - The primary threats to the Ash Meadows Amargosa pupfish consist of habitat destruction and the inimical effects of exotic fish introductions. Physical alteration of habitats has consisted of 1) pumping of spring water for agricultural uses (which caused water Management Practices - 1 (DRAFT) - Management Practices Species PUPFISH, AMARGOSA, ASH MEADOWS Species Id ESIS251003 Date 14 MAR 96 level fluctuations serious enough to completely drain at least one area and cause a local extirpation), 2) channelization of spring outflows, 3) clearing of riparian vegetation, 4) cementing channels, and 5) draining marshes and creeks See references (01,02,06,13,15,16,17,18,19). The introduction and establishment of a variety of exotic fishes (some stocked into downstream reservoirs) has also threatened the pupfish (02,03,04,06,15,16,17,18,19). The exact mechanisms by which exotic fishes affect the pupfish are uncertain, but some species (e.g., largemouth bass, arawana) undoubtedly prey on the pupfish, while others (e.g., sailfin mollies, mosquitofish) probably compete with the pupfish. The introduction of exotic parasites and/or diseases, or an increased susceptibility to indigenous parasites, is also a problem when exotic fishes are introduced (02). Trampling by people, horses, and free-roaming burrows caused gradual degradation of the spring and outlet stream banks. These processes denuded the edge of the spring pool and destroy the structure of the outlet stream. Tamarisk, an introduced species not native to the spring system, may have adversely impacted the habitat, especially the outlet streams (39). At the time of listing, existing State regulations were not adequate to counter the threats to the species (40). Because of the recent acquisition of many spring areas by the U.S. Fish and Wildlife Service, the major threats in the future will most likely consist of additional exotic species introductions rather than physical habitat alteration. Because of the changes in land ownership, a major residential and commercial development planned for Ash Meadows will not be built and is no longer a threat (13,16,19). UNAPPROVED PLAN: U.S. Fish and Wildlife Service. 1986. Recovery Plan for the Endangered and Threatened Species of Ash Meadows, Nevada. Agency Draft. U.S. Fish and Wildlife Service, Portland, OR. 169 pp. The primary objective in recovery of the Ash Meadows Amargosa pupfish is to reclassify the endangered populations to non-listed status, and will be accomplished by restoring and protecting habitat which can support viable, self-sustaining populations. The size and extent of populations needs to be determined; the habitat occurs within the Ash Meadows essential habitat. Reclassification may be considered when threats have been removed and when habitats/populations have been restored for a period of ten years and to specified levels (as determined through research). The general criteria for reclassifying/delisting the Ash Meadows Amargosa pupfish is summarized as follows: 1. Protect the Ash Meadows aquifer to maintain the current (January 1986) spring discharge (requires maintaining or controlling the water table). Management Practices - 2 (DRAFT) - Management Practices Species PUPFISH, AMARGOSA, ASH MEADOWS Species Id ESIS251003 Date 14 MAR 96 2. Maintain each population within all of its historic habitat at a size to be detrmined by research. 3. Secure and withdraw habitats and incorporate them into Ash Meadows NWR (including land and water right acquisition). Adverse development such as road construction must be prohibited. Restricting the use of off-road vehicles and some camping sites may be necessary, along with prohibiting swimming, fishing and hiking in some areas. 4. Eliminate introduced species (including species of crayfish, bullfrog, snails, fish, wild horses, and plants). 5. Reconstruct habitat, plant communities and aquatic communities, and reestablish populations throughout its native range. 6. Enforce all State and Federal laws and regulations. 7. Implement a public information and education program. Management Practices - 3
                                   (DRAFT) - References
                          Species PUPFISH, AMARGOSA, ASH MEADOWS
                                  Species Id ESIS251003
                                      Date 14 MAR 96



     

References

***** REFERENCES FOR ALL NARRATIVES EXCEPT N-OCCURRENCE ***** 01 Bateman, R.L., A.L. Mindling, and R.L. Naff. 1974. Development and management of ground water in relation to preservation of desert pupfish in Ash Meadows, southern Nevada. Tech. Rept. Ser. H-W, Center for Water Resour. Res. Publ. 17, Univ. Nevada, Reno. 02 Deacon, J.E. 1979. Endangered and threatened fishes of the West. Great Basin Nat. Mem. 3:41-64. 03 Deacon, J.E. and J.E. Williams. 1984. Annotated list of the fishes of Nevada. Proc. Biol. Soc. Wash. 97:103-118. 04 Deacon, J.E., C. Hubbs, and B.J. Zahuranec. 1964. Some effects of introduced fishes on the native fish fauna of southern Nevada. Copeia 1964:384-388. 05 Eigenmann, C.H. and R.S. Eigenmann. 1889. Description of a new species of Cyprinodon. Proc. Calif. Acad. Sci. 1:270. 06 Hardy, T. 1980. The Inter-basin area report - 1979. Proc. Desert Fishes Council 11:5-21. 07 Kodric-Brown, A. 1981. Variable breeding systems in pupfishes (genus Cyprinodon): Adaptations to changing environments. Pages 205-235. IN: Fishes in North American deserts. R.J. Naiman and D.L. Soltz, eds. Wiley-Interscience, New York. 08 La Rivers, I. 1962. Fishes and fisheries of Nevada. Nev. State Fish and Game Comm. 782 pp. 09 Miller, R.R. 1943. The status of Cyprinodon macularius and Cyprinodon nevadensis, two desert fishes of western North America. Occ. Pap. Mus. Zool., Univ. Mich. 473:1-25. 10 Miller, R.R. 1948. The cyprinodont fishes of the Death Valley system of eastern California and southwestern Nevada. Misc. Publ. Mus. Zool., Univ. Mich. 68:1-155. 11 Moyle, P.B. 1976. Inland fishes of California. Univ. Calif. Press, Berkeley. 405 pp. 12 Naiman, R.J. 1979. Preliminary food studies of Cyprinodon macularius and Cyprinodon nevadensis (Cyprinodontidae). Southwest. Nat. 24:538-541. 13 Sanchez, P.G. 1981. Ash Meadows scenario. Cal-Neva Wild. Trans. 1981:144-147. 14 Soltz, D.L. 1974. Variation in life history and social organization of sume populations of Nevada pupfish, Cyprinodon nevadensis. Ph.D. Dissertation, Univ. Calif., Los Angeles. 15 Soltz, D.L. and R.J. Naiman. 1978. The natural history of the native fishes in the Death Valley system. Nat. Hist. Mus. Los Angeles Co. 30:1-76. 16 Williams, C.D. 1984. The decline of Ash Meadows, a unique desert ecosystem. Proc. Calif. Ripar. Syst. Conf. 1984:716-719. 17 Williams, J.E. and D.W. Sada. 1985. Status of two endangered fishes, Cyprinodon nevadensis mionectes and Rhinichthys osculus nevadensis, from two springs in Ash Meadows, Nevada. Southwest. Nat. 30(4):475-484. 18 Deacon, J.E., G. Kobetich, J.D. Williams, S. Conteras, et al. 1979. Fishes of North America, endangered, threatened, or of special concern: 1979. Fisheries 4:29-44. References - 1 (DRAFT) - References Species PUPFISH, AMARGOSA, ASH MEADOWS Species Id ESIS251003 Date 14 MAR 96 19 Cook, S.R. and C.D. Williams. 1982. The status and future of Ash Meadows, Nye County, Nevada. Office of the Attorney general, State of Nevada, Carson City, NV. 20 Deacon, J.E. 1968. Ecological studies of aquatic habitats in Death Valley National Monument, with special reference to Saratoga Springs. Final Rept. under Contract No. 14-10-0434-1989. 82 pp. 21 Brown, J.H. and C.R. Feldmeth. 1971. Evolution in constant and fluctuating environments: Thermal tolerances of desert pupfish (Cyprinodon). Evolution 25:390-398. 22 Hirshfield, M.F., C.R. Feldmeth, and D.L. Soltz. 1980. Genetic differences in physiological tolerances of Amargosa pupfish (Cyprinodon nevadensis) populations. Science 207:999-1001. 23 Feldmeth, C.R. 1981. The evolution of thermal tolerance in desert pupfish (Genus Cyprinodon). Pages 357-384. IN: Fishes in North American deserts. R.J. Naiman, and D.L. Soltz, eds. Wiley-Interscience, New York. 24 Naiman, R.J. 1976. Productivity of a herbivorous pupfish population (Cyprinodon nevadensis) in a warm desert stream. J. Fish Biol. 9:125-137. 25 Deacon, J.E. 1967. The ecology of Saratoga Springs Death Valley National Monument. Final Rept. under Contract No. 14-10-0434-0989. 26 Deacon, J.E. and W.L. Minckley. 1974. Desert Fishes. Pages 385-487. IN: Desert Biology, Vol. 2. G.W. Brown, Jr., ed. Academic Press, New York. 27 Brown, J.H. 1971. The desert pupfish. Sci. Am. 225:104-110. 28 Winograd, I.J. and W. Thordarson. 1975. Hydrogeologic and hydrochemical framework, south-central Great Basin, Nevada-California, with special reference to the Nevada Test Site. Geol. Surv. Prof. Pap. 712-C, 1-126. 29 Hillyard, S.D. 1981. Energy metabolism and osmoregulation in desert fishes. Pages 385-409. IN: Fishes in North American deserts. R.J. Naiman and D.L. Soltz, eds. Wiley-Interscience, New York. 30 Soltz, D.L. and M.F. Hirschfield. 1981. Genetic differentiation of pupfishes (Genus Cyprinodon) in the American southwest. Pages 291-333. IN: Fishes in North American Deserts. R.J. Naiman and D.L. Soltz, eds. Wiley-Interscience, New York. 31 Beatley, J.C. 1978. Ash Meadows: Nevada's unique oasis in the Mojave Desert. Mentzelia 3:20--24. 32 Williams, Jack E. 1985. Personal observations. 2800 Cottage Way, Room E-1823, Sacramento, CA 95825. 33 Bateman, R.L., A.L. Mindling, and R.L. Naff. 1974. Development and management of ground water in relation to preservation of desert pupfish in Ash Meadows, southern Nevada. Univ. Nev. Desert Res. Inst., Tech. Rept. Ser. H-W Hydro. Water Res. Publ. No. 17:1-39. 34 Dudley, W.W., Jr. and J.D Larson. 1976. Effect of irigation pumping on desert pupfish habitats in Ash Meadows, Nye Co., Nevada. U.S. Geol. Surv. Prof. Pap. 927:1-52. 35 Walker, G.E. and T.E. Eakin. 1963. Geology and ground water at Amargosa Desert, Nevada-California. Nevada Dept. Conserv. Nat. Res., Water Res. Reconn. Ser. Rept. 14:1-45. 36 Worts, G.F., Jr. 1963. Effect of ground water development on the References - 2 (DRAFT) - References Species PUPFISH, AMARGOSA, ASH MEADOWS Species Id ESIS251003 Date 14 MAR 96 pool level in Devil's Hole, Death Valley National Monument, Nye Co., Nevada. U.S. Geol. Surv., Water Res. Div., Carson City, NV. 27 pp. 37 Baugh T.M. and J.E. Deacon. 1983. Maintaining the Devil's Hole pupfish, Cyprinodon diabolis Wales in aquaria. J. Aquariculture Aquat. Sci. 3(4):73-75. 38 Lee, D.S., et al. 1980. Atlas of North American freshwater fishes. N.C. State Mus. Nat. History. 854 pp. 39 Detweiler, K.G. 1980. Intensive inventory and analysis Ash Meadows wildlife habitat area. Report N5-WHA-A1. Stateline - Esmeralda Resource Area, Las Vegas District, Bur. Land Mgmt., NV. April 21, 1980. 56 pp + appendices. 40 U.S. Fish and Wildlife Service. September 2, 1983. Determination of Endangered status and Critical Habitat for two fish species; Ash Meadows, Nevada. Fed. Reg. 48(172):40178-40186. ***** REFERENCES FOR N-OCCURRENCE NARRATIVE ONLY ***** 01 Baugh, T., J.E. Williams, D. Buck, and J.E. Deacon. 1986. New distributional records for Cyprinodon nevadensis mionectes, an endangered pupfish from Ash Meadows, Nevada. Southwest. Nat. 31(4):544-546. 02 Deacon, J.E. and J.E. Williams. 1984. Annotated list of the fishes of Nevada. Proc. Biol. Soc. Wash. 97:103-118. 03 Hardy, T. 1980. The Inter-basin area report - 1979. Proc. Desert Fishes Council. 11:5-21. 04 La Rivers, I. 1962. Fishes and fisheries of Nevada. Nev. State Fish and Game Comm. 782 pp. 05 Miller, R.R. 1948. The cyprinodont fishes of the Death Valley system of eastern California and southwestern Nevada. Misc. Publ. Mus. Zool. Univ. Mich. 68:1-155. 06 Soltz, D.L. and R.J. Naiman. 1978. The natural history of the native fishes in the Death Valley system. Nat. Hist. Mus. Los Angeles Co. 30:1-76. 07 Williams, J.E. and D.W. Sada. 1985. Status of two endangered fishes, Cyprinodon nevadensis mionectes and Rhinichthys osculus nevadensis, from two springs in Ash Meadows, Nevada. Southwest. Nat. 30(4):475-484. 08 Cook, S.R. and C.D. Williams. 1982. The status and future of Ash Meadows, Nye County, Nevada. Office of the Attorney general, State of Nevada, Carson City, NV. References - 3