(DRAFT) - Taxonomy
                            Species PUPFISH, DEVILS HOLE
                                Species Id ESIS251005
                                   Date 14 MAR 96



TAXONOMY

NAME - PUPFISH, DEVILS HOLE OTHER COMMON NAMES - PUPFISH, DEVILS HOLE; PUPFISH, DEVIL'S HOLE; PUPFISH and DEVIL ELEMENT CODE - CATEGORY - Fish PHYLUM AND SUBPHYLUM - CHORDATA, CLASS AND SUBCLASS - OSTEICHTHYES, ORDER AND SUBORDER - ANTHERINIFORMES, FAMILY AND SUBFAMILY - CYPRINODONTIDAE, GENUS AND SUBGENUS - CYPRINODON, SPECIES AND SSP - DIABOLIS, SCIENTIFIC NAME - CYPRINODON DIABOLIS AUTHORITY - TAXONOMY REFERENCES - COMMENTS ON TAXONOMY - Devils Hole Pupfish Cyprinodon diabolis Wales, 1930 KINGDOM: Animal GROUP: Fish PHYLUM: Chordata CLASS: Osteichthyes ORDER: Antheriniformes FAMILY: Cyprinodontidae Cyprinodon diabolis Wales, the Devils Hole pupfish, was originally described (01) as having a body subovate to the posterior ends of dorsal and anal bases, rectangular caudal peduncle, top of head flat, mouth on the level with the caudal peduncle, premaxillary below the middle of the eye, single series of teeth on jaws, 16 teeth in both upper and lower jaw, 16 gill rakers, ctenoid scales, lateral median row of scales =27, 7 rows of pores along the border of the preopercle, 17 pectoral rays, 12 dorsal rays, 28 caudal rays, absence of pelvic fins. In addition, the length of the three cusps on the teeth are about equal with the middle cusps slightly wider than the lateral cusps. The holotype and paratypes were collected by Joseph Wales from Devils Hole on 26 March 1930. The holotype and seven paratypes are maintained at the California Academy of Sciences, San Francisco, CA, and are designated as follows: Taxonomy - 1 (DRAFT) - Taxonomy Species PUPFISH, DEVILS HOLE Species Id ESIS251005 Date 14 MAR 96 Holotype SU23928 Paratypes SU23929 Miller (13) subsequently complete a more detailed study that established the Devils Hole pupfish as the most distinctive species in the genus Cyprinodon. Miller reports the species to be unique for its lack of pelvic fins, convex caudal fin, absence of preorbital scales, and absence of vertical cross bars in mature females. The posteriorly placed dorsal fin, short caudal peduncle, large head and eyes, wide mouth and long anal fin are also distinctive. Other common names used for this species include Devil's Hole pupfish and devil pupfish. Substantial collections of Devils Hole pupfish are maintained at the University of Michigan Museum of Zoology. Taxonomy - 2
                                  (DRAFT) - Status
                            Species PUPFISH, DEVILS HOLE
                                Species Id ESIS251005
                                   Date 14 MAR 96



STATUS

Coded Status E: Federal Endangered Non-consumptive recreational COMMENTS ON STATUS - U.S. STATUSES AND LAWS: The Devils Hole pupfish (Cyprinodon diabolis) has been designated an Endangered species pursuant to the Endangered Species Act of 1973 (50 CFR 17.11; P.L. 93-205, 87 Stat. 884; 16 U.S.C. 1531-1540), as amended. The species has this status wherever found including the State of Nevada. No Critical Habitat has been designated. This species is protected by the Lacey Act (P.L. 97-79, as amended; 16 U.S.C. 3371 et seq.) which makes it unlawful to import, export, transport, sell, receive, acquire, or purchase any wild animal (alive or dead including parts, products, eggs, or offspring): (1) in interstate or foreign commerce if taken, possessed, transported or sold in violation of any State law or regulation; or (2) if taken or possessed in violation of any U.S. law, treaty, or regulation or in violation of Indian tribal law. It is also unlawful to possess any wild animal (alive or dead including parts, products, eggs, and offspring) within the U.S. territorial or special maritime jurisdiction (as defined in 18 U.S.C. 7) that is taken, possessed, transported, or sold in violation of any State law or regulation, foreign law, or Indian tribal law. RESPONSIBLE FEDERAL AGENCIES: USFWS -Responsible for the management/recovery, listing, and law enforcement/protection of this species. NPS -Responsible for the law enforcement/protection of this species with applicable State and Federal laws on public lands under their control. Also responsible for conservation (Nat. Park System Organic Act - 16 U.S.C. 1, 2-3)/management/recovery on National Park Service lands. Taking, possessing, or disturbing of Federally listed species is prohibited on NPS lands (36 CFR 2.1, 2.2, and 2.3). All Federal agencies have responsibility to ensure that any action authorized, funded, or carried out by that agency is not likely to jeopardize the continued existence of the species or result in the destruction or adverse modification of Critical Habitat (50 CFR 402), and to utilize their authorities to carry out programs for the Status - 1 (DRAFT) - Status Species PUPFISH, DEVILS HOLE Species Id ESIS251005 Date 14 MAR 96 conservation of the species. STATE STATUSES AND LAWS: STATE: Nevada DESIGNATED STATUS: Protected, Endangered ADMINISTRATIVE AGENCY: Nevada Department of Wildlife STATE STATUTE: Nevada Administrative Code 503.015 to 503.080. INTERNATIONAL STATUSES, TREATIES, AND AGREEMENTS: Cyprinodon diabolis is listed as Endangered in the IUCN Red Data Book, Vol. 4 (1977). It is also listed by the U.S. in the Convention on Nature Protection and Wildlife Preservation in the Western Hemisphere Annex (1970). ECONOMIC STATUSES: As a species within the Ash Meadows National Wildlife Refuge occupying a unique and fragile habitat, the Devils Hole pupfish has educational and interpretive value. 67/03/11:32 FR 04001/ - Listed as Endangered 79/05/21:44 FR 29566/29577 - Status review 85/07/22:50 FR 29901/29909 - Five year review 87/07/07:52 FR 25522/ - Five year review completion Status - 2
     

HABITAT ASSOCIATIONS

HABITAT - AQUATIC INLAND AQUATIC LAND USE - Cropland and Pasture Herbaceous Rangeland Shrub and Brush Rangeland Nonforested Wetland Bare Exposed Rock NATIONAL WETLAND INVENTORY CODES NWI NWICLS NWIMOD NWISPEC Lacustrine, littoral UB4 Lacustrine, littoral UB3 2UB Lacustrine, littoral UB1 Lacustrine, littoral RS2 Lacustrine, littoral RS1 Lacustrine, littoral RB2 Lacustrine, littoral RB1 Lacustrine, littoral AB1 Lacustrine, limnetic RB2 Lacustrine, limnetic RB1 Lacustrine, limnetic UB1 Lacustrine, limnetic AB1 Lacustrine, limnetic OW0 COMMENTS ON HABITAT ASSOCIATIONS - Ash Meadows is a large oasis in southwestern Nevada. Its nearly 50 seeps and springs discharge about 17,000 acre-feet of water annually (21,25). This water formerly flowed into an extensive marsh which was drained in the mid 1960's. Thunderstorms occasionally caused floodwaters to discharge from Ash Meadows into the Amargosa River which terminates in the floor of Death Valley. Ash Meadows is home to some 30 species of endemic plants, insects, snails, other invertebrates and fish. Human development in the area consists primarily of small scattered residences with which subsistence gardens, small orchards or agricultural fields may be associated. During the early 1970's a large-scale farm began operating in Ash Meadows. Development of the large farm involved extensive removal of natural vegetation, land leveling, construction of irrigation wells, ditches and fences and other activities necessary for commercial farming (24,26). Primary crops grown included alfalfa, cotton and sorghum. Cattle were grazed on developed and irrigated pasture. A U.S. Supreme Court decision limited groundwater pumping by the large farm in 1976 (22). The land was sold to a developer who continued farming on a smaller scale while preparing the land for subdivision development. The 12,663 acre subdivision, after extensive efforts by conservationists, was purchased by The Nature Conservancy in 1984 and then resold to the U.S. Fish and Wildlife Service (USFWS). The USFWS plans to restore the area for use as a Wildlife Refuge (23). Currently associated agricultural uses are limited to a small orchard and fallow field. Natural vegetation on the nearly level terrain near the springs is dominated by groves of ash trees (Fraxinus velutina var. coriacea) and screwbean (Prosopis pubescens) in association with a large scrub Habitat Associations - 1 (Baccharis emoryi). Sand dunes in the area are donimated by mesquite (Prosopis glandulosa var torreyana). Shadscale (Atriplex confertifolia) and Haplopappus (Haplopappus acradenius) dominate areas away from direct influence of the spring waters. Large areas of seasonally wet, salt-encrusted soils are covered with saltgrass (Distichlis spicata). Greasewood (Larrea sp.) dominates the better drained soils on the surrounding slopes. The Ash Meadows Amargosa pupfish (Cyprinodon nevadensis mionectes) and the Ash Meadows speckled dace (Rhinichthys osculus nevadensis) occupied a large number of the springs and outflow streams and remnants of the large marsh at lowest elevations in Ash Meadows. The Warm Springs pupfish (Cyprinodon nevadensis pectoralis) lives in a few springs at slightly higher elevation. Warm Springs pupfish habitats have been altered relatively little, because they were never included in the large commercial farm or in the area targeted for subdivision development. A private residence, road, culverts and fencing are obvious features that today alter the natural water courses. Some grazing has occurred in the area, but BLM is in the process of removing feral horses from the area. Devils Hole is a rock-bound pool about 2.2 x 3.5 meters lying 15 meters deep in a range of low hills in Ash Meadows, Amargosa Township, Nye Co., NV. This pool is composed of essentially two components including a rock shelf 3.5 x 5 x 0.3 meters deep and a connected deeper section about 3.5 x 17 meters to unknown depth. Devils Hole pupfish occupy the uper 80 feet of the portion of the cavern system open to daylight. About 50% of the population occurs over the small, shallow rock shelf (07). The temperature in Devils Hole remains a uniform 32.8 - 33.9 degrees C. Devils Hole has the highest mean temperature of all the springs in the Ash Meadows area (03). This species is capable of tolerating temperatures up to 42.7 degrees C depending on the prior acclimation temperature (04). Dissolved oxygen varies between 2-8 ppm and increases daily as sunlight strikes the water and the photosynthesis of algae increases. Seasonal variation results from greater or lesser periods of sunlight exposures. Recent nutrient measurements at Devils Hole indicate the following values for the elements and compounds measured: NUTRIENT DATA (mg/l) ---------------------------------------------------------------------- Phosphorus DATE DEPTH TOTAL DISSOLVED NO3 NO2 NH3 TKN SOURCE ---------------------------------------------------------------------- 1953 S .50 Worts, 1963 1/22/63 S .50 21 10/21/63 S .00 .3 21 12/9/66 S .00 .2 21 6/28/72 S .04 .5 21 6/28/72 50FT .04 .5 21 6/28/72 100FT .04 .2 21 1974 S .2 24 10/23/82 S .2 22 3/23/83 80FT .014 .002 .19 .024 .03 .28 USBR 4/22/83 S .024 .003 .13 .013 .03 .19 USBR 4/22/83 80FT .009 1/ .15 .009 .02 .29 USBR 3/17/84 S .011 .003 .30 1/ 2/ .06 USBR 3/17/84 S .014 1/ .30 1/ 2/ .21 USBR 3/17/84 80FT .012 .007 .31 1/ 2/ .07 USBR 8/24/84 S .012 1/ .31 1/ 2/ .13 USBR 6/3/84 S .001 .049 USGS 6/3/84 15FT .002 .031 USGS Habitat Associations - 2 ---------------------------------------------------------------------- 1/ Below the detection limit of 0.001 mg/l 2/ Below the detection limit of 0.01 mg/l Under DEPTH, S=Surface ---------------------------------------------------------------------- At the Hoover Dam Desert Fishes Refugium, Devils Hole pupfish are located in a concrete structure set partially in the ground, described by Williams (05) as being 5.79 m long, 1.07 m deep at the shallow end and 3.15 m deep at the deep end. This description also fits the Amargosa Pupfish Refugium. Temperature in both refugia is nearly always cooler than in Devils Hole. Usually the temperatures remain near 29.5 degrees C but occasionally when the water supply system fails temperatures fall markedly. The larger size attained by refugium fish may be primarily a result of living at cooler temperatures. Oxygen concentration in the refugia varies between about 4-6 ppm (17). Habitat Associations - 3
                                (DRAFT) - Food Habits
                            Species PUPFISH, DEVILS HOLE
                                Species Id ESIS251005
                                   Date 14 MAR 96



FOOD HABITS

TROPHIC LEVEL - OMNIVORE LIFESTAGE FOOD FOOD PART General Arthropods General Crustaceans General Molluscs General Worms General Detritus General Diatoms General Algae Food Habits - 1
                         (DRAFT) - Environment Associations
                            Species PUPFISH, DEVILS HOLE
                                Species Id ESIS251005
                                   Date 14 MAR 96



ENVIRONMENTAL ASSOCIATIONS

G = General A = Adult LIM = Limiting RA = Resting Adult J = Juvenile FA = Feeding Adult RJ = Resting Juvenile BA = Breeding Adult FJ = Feeding Juvenile P = Pupae L = Larvae E = Egg RL = Resting Larvae FL = Feeding Larvae
LIFESTAGE ENVIRONMENTAL ASSOCIATIONS G Aquatic Features: Pool areas G Terrestrial Features: Caves, wet G Terrestrial Features: Rock outcrops Environment Associations - 1
                               (DRAFT) - Life History
                            Species PUPFISH, DEVILS HOLE
                                Species Id ESIS251005
                                   Date 14 MAR 96



LIFE HISTORY

FOOD HABITS: Minckley and Deacon (06) described the food habits of Cyprinodon diabolis. The fish is an opportunistic feeder with inorganic particulate matter appearing most frequently and in the greatest volume in gut contents throughout the year. The green algae Spirogyra and various diatoms were about equal in occurrence but varied seasonally in a reciprocal manner with diatoms being most important in winter and spring. The amphipod Hyalella was present more frequently than either an unidentified ostracod (crustacean) or protozoans which were found at about the same frequency. Other endemic organisms found in the gut contents in small numbers were the elmid beetle Stenelmis, a tubullarian, Dugesia, and a hydrobiid snail, Tryonia. HOME RANGE/TERRITORY: The Devils Hole pupfish is restricted to the top 27 meters of Devils Hole and to the Amargosa Pupfish Refugium, both in Nye Co., and to the Hoover Dam Desert Fishes Refugium, Clark Co. (07,13,18). Devils Hole has an overall surface area of about 3.5 x 22 m. Each refugium is about 5.8 x 3 m. This species is essentially non-territorial although, under certain conditions, it may exhibit ephemeral territoriality in defending egg deposition areas. Baugh (08) has found that males of this species maintain 30 discrete behaviors in aquaria and females 18 discrete bahaviors in aquaria. PERIODICITY: Baugh and Deacon (07) have demonstrated daily and yearly movement of this species within the water column of Devils Hole. More pupfish use the surface water, on a daily and yearly basis, when the intensity of sunlight on the water is less intense than when it is more intense. More than 60% of the population is usually found in the upper 3 m of water. MIGRATION PATTERNS: In the conventional sense, the Devils Hole pupfish is non-migratory. COVER/SHELTER REQUIREMENTS: In 1976, the U.S. District Court (U.S. vs Cappaert) established the minimum water level necessary in Devils Hole to maintain this species at 2.7 feet below a USGS datum point on the rock wall above maximum water level (09). Due to its legal status, this must remain the adjudicated standard for habitat requirements. Biological evidence continues to support the conclusion that the water level must remain above 2.7 to insure the survival of the species in Devils Hole. REPRODUCTIVE SITE REQUIREMENTS: The area in Devils Hole called the rock shelf (07) is considered the required habitat for reproduction. This is a rock-bound area about 3.5 x 3 meters with an average water depth of about 0.3 meters. The base substrate is composed of limestone. The limestone is covered with a thin layer of silt, sand, and detritus over which are scattered Life History - 1 (DRAFT) - Life History Species PUPFISH, DEVILS HOLE Species Id ESIS251005 Date 14 MAR 96 small rocks of various sizes. The algae Spirogyra grows over the rock shelf. C. diabolis uses this algae and the silt, sand, detritus substrate as a spawning medium. In aquaria, this species has been observed to spawn on rock (08). REPRODUCTIVE CHARACTERISTICS: Size at sexual maturity is about 14 mm (SL) for females (19). James (12) indicates that males mature at about 14-16 mm (TL). Spring and summer growth data from the natural population indicates that mature sizes can be achieved in about 8-10 weeks in Devils Hole (12). While some reproduction can occur throughout the year under the right conditions, most spawning actually occurs at about 18-24 months. The mating relationship has been defined as a consort-pair in which one or more males follow one female (10). Baugh (08) has defined the reproductive behaviors, sequences, and frequencies for this species. There are eight male reproductive behaviors out of a total of 30 male behaviors. Minckley and Deacon (19) described some aspects of the reproductive biology of C. diabolis. Ovaries and ova are small during the winter, increase in relative weight until May and then decline. Considerable variability in mean ova diameter occurs with diameters higher in May than during other months. Considering the percentage distribution of ova diameters, it is apparent that more larger ova are present in May than at other times and that during August, October, and November Class I ova (400-900 microns) are absent. In general, reproductive capability increases in the spring. Pairing for reproduction lasts from a few seconds to, perhaps a minute. Eggs probably hatch in about 7 days (20). PARENTAL CARE: This species does not engage in parental care. POPULATION BIOLOGY: The population size of C. diabolis varies by time of year. The adult population in Devils Hole may drop below 200 fish from January to April and may exceed 500 fish from August to October (11). James (12) reports male to female sex ratios which vary from 3:1 in January to 1:2 in July. Deacon and Baugh (11) have stated that, given sufficient space, the fish in Devils Hole become food-limited. Annual and seasonal variations in population size appear attributable to variations in algae productivity (11). Predictable seasonal variations in sunlight occur because of the position of Devils Hole in the mountain. Annual variations in algal productivity are considerable and appear to be attributable to unpredictable annual variations in allochthrous nutrients supplied to Devils Hole. Allochthonous nutrients of sufficient quantity supplied for a long enough period of time appear to have a marked impact on algal productivity. Owl castings and feces are a main source of these nutrients. Some nutrients may also be supplied by water inflow during infrequent intense rainfall (11). SPECIES INTERRELATIONSHIPS: None. Life History - 2 (DRAFT) - Life History Species PUPFISH, DEVILS HOLE Species Id ESIS251005 Date 14 MAR 96 OTHER LIFE HISTORY DESCRIPTORS: In spite of the constant temperature environment of Devils Hole, James (12) has shown that spring and summer growth rates are faster than growth rates achieved during winter. This may reflect food limitation during winter. Life History - 3
                           (DRAFT) - Management Practices
                            Species PUPFISH, DEVILS HOLE
                                Species Id ESIS251005
                                   Date 14 MAR 96



MANAGEMENT PRACTICES

RESULT MANAGEMENT PRACTICE Beneficial Controlling water levels Beneficial Land Acquisition Beneficial Water Right Acquisition Beneficial Stocking captive-reared wild-strain animals Adverse Harassment/Vandalism/Indiscriminate Killing Existing Harassment/Vandalism/Indiscriminate Killing Adverse Food Supply Reduction Existing Food Supply Reduction Adverse Water Level Fluctuation Existing Water Level Fluctuation Adverse Groundwater drawdown Existing Groundwater drawdown Adverse Existing COMMENTS ON MANAGEMENT PRACTICES - The Devils Hole pupfish was listed as Endangered in 1967 with the first listing of endangered fishes in the U.S. At that time there were no evident threats to the population. Its endangered status was based solely on its very restricted distribution. In 1969 pumping of ground water in the Ash Meadows area near Devils Hole resulted in a trend of a declining water level in Devils Hole which reached a low point of 1.19 m below a USGS reference point on 20 September 1972. Unusually low water levels in Devils Hole correspond to unusually low population sizes of Devils Hole pupfish as well as atypical seasonal variations in population size (02,14). Litigation in U.S. District Court, U.S. Court of Appeals and U.S. Supreme Court in U.S. vs Cappaert established a minimum water level of 1.01 m below the reference point on June 5, 1973. On 2 July 1976, that minimum was raised to .91 m and in 1977 to .82 m (02,14). Each court-ordered increase in the minimum water level allowed in Devils Hole required a corresponding reduction in ground water pumping for irrigation in the nearby area in Ash Meadows. Irrigation in the area was virtually stopped in 1984 when The Nature Conservancy purchased 12,663 acres in the Ash Meadows from Preferred Equities, a land development company (15). The land was later sold to the U.S. Fish and Wildlife Service for use as a wildlife refuge. Population size of Devils Hole pupfish fluctuates annually from a low in March and April to a high in August and September (02,11,14). Low water levels in the past have depressed both maximum and minimum population sizes. The higher water levels of the past five years have not limited population sizes. Presently, variations in algal productivity appear to exert the principal limitation to population size, both seasonally and annually (11). Relatively poor algal productivity in 1983 and 1984 has been followed by the lowest spring populations since 1975 (16). The major threat to survival of Devils Hole pupfish over the past 15 years has been habitat disruption from lowering water levels. Management Practices - 1 (DRAFT) - Management Practices Species PUPFISH, DEVILS HOLE Species Id ESIS251005 Date 14 MAR 96 Management Practices - 2 (DRAFT) - Management Practices Species PUPFISH, DEVILS HOLE Species Id ESIS251005 Date 14 MAR 96 Establishment of the Ash Meadows National Wildlife Refuge has effectively removed that threat. At present, the greatest threat to the Devils Hole pupfish is vandalism by the general public. There have been incidences of people breaking into the Devil's Hole area and causing a degradation of the immediately surrounding habitat. There have also been threats of intentionally pouring toxicants into Devil's Hole. The original reason for listing the species as endangered, restricted range, still applies. UNAPPROVED PLAN: U.S. Fish and Wildlife Service. 1986. Recovery Plan for the Endangered and Threatened Species of Ash Meadows, Nevada, Draft. U.S. Fish and Wildlife Service, Portland, OR. 169 pp. The Devils Hole pupfish is included in the "Recovery Plan for the Endangered and Threatened Species of Ash Meadows, Nevada". The primary objective of the plan is to reclassify the endangered/threatened populations to non-listed status, and will be accomplished by restoring and protecting habitat which can support viable, self-sustaining populations. The size and extent of populations needs to be determined for most species, and the habitats of all species, except the spring-loving centaury, occur within the Ash Meadows essential habitat. Except for the Devils Hole pupfish and Warm Springs pupfish, reclassification may be considered when threats have been removed and when habitats/populations have been restored for a period of ten years and to specified levels (as determined through research); recovery of the Devils Hole and Warm Springs pupfish will be considered when their recovery tasks have been accomplished. The general criteria for reclassifying/delisting the Devils Hole pupfish is summarized as follows: 1. Protect the Ash Meadows aquifer to return the level at Devil's Hole to 1.42 feet above the copper reference point and maintain a population of not less than 300 individuals during the winter and 700-900 during the late summer and early autumn. Rights to surface waters in private ownership must be purchased to maintain the viability of the Ash Meadows aquifer. 2. Secure and protect Devil's Hole. Various management responsibilities may be implemented to designate the FWS as a management cooperator with the NPS, which has legal responsibility for 40 acres surrounding Devil's Hole. 3. Establish and protect the captively propagated refugium populations at Hoover Dam and Amargosa Pupfish Refugium. 4. Monitor populations. 5. Enforce State and Federal laws and regulations. 6. Establish a public information and education program. Management Practices - 3 (DRAFT) - Management Practices Species PUPFISH, DEVILS HOLE Species Id ESIS251005 Date 14 MAR 96 Management Practices - 4
                                   (DRAFT) - References
                               Species PUPFISH, DEVILS HOLE
                                  Species Id ESIS251005
                                      Date 14 MAR 96



     

References

***** REFERENCES FOR ALL NARRATIVES EXCEPT N-OCCURRENCE ***** 01 Wales, J.H. 1930. Biometrical studies of some races of cyprinodont fishes from the Death Valley region, with description of Cyprinodon diabolis, n. sp. Copeia 1930:61-70. 02 Deacon, J.E. and M.S. Deacon. 1979. Research on endangered fishes in the National Parks with special emphasis on the Devils Hole pupfish. Proc. 1st Conf. Scientific Res. in Natl. Parks 1:9-19. 03 Soltz, D.L. and R.J. Naiman. 1978. The natural history of the native fishes in the Death Valley system. Nat. Hist. Mus. Los Angeles Co. 30:1-76. 04 Brown, J.H. and C.R. Feldmeth. 1971. Evolution in constant and fluctuating environments. Evolution 25:390-398. 05 Williams, J.E. 1977. Observations on the status of the Devil's Hole pupfish in the Hoover Dam Refugum. USDI, Bur. Recl., REC ERC 77-11. 15 pp. 06 Minckley, C.O. and J.E. Deacon. 1975. Foods of the Devil's Hole pupfish, Cyprinodon diabolis (Cyprinodontidae). Southwest. Nat. 20(1):26-29. 07 Baugh, T.M. and J.E. Deacon. 1983. Daily and yearly movement of the Devil's Hole pupfish Cyprinodon diabolis Wales in Devil's Hole, Nevada. Great Basin Nat. 43(4):592-596. 08 Baugh, T.M. 1985. Patterns of behavior of the Devil's Hole pupfish Cyprinodon diabolis Wales in aquaria. On file at Dept. Biol. Sci., Univ. Nevada, Las Vegas. 09 U.S. Fish and Wildlife Service. 1980. Devil's Hole pupfish Recovery Plan. U.S. Fish & Wildlife Serv., Portland, OR. 169 pp. 10 Kodric-Brown, A. 1981. Variable breeding systems in pupfishes (Genus Cyprinodon): Adaptations to changing environments. Pages 205-235. IN: Fishes in North American deserts. R.J. Naiman and D.L. Soltz, eds. John Wiley & Sons, New York. 11 Deacon, J.E. and T.M. Baugh. 1985. Population fluctuations of the Devil's Hole pupfish - 1972-1984. Rept. on file with Superintendent, Death Valley National Monument, CA. 12 James, C.J. 1969. Aspects of the ecology of the Devil's Hole pupfish, Cyprinodon diabolis Wales. M.S. Thesis, Univ. Nev., Las Vegas. 70 pp. 13 Miller, R.R. 1948. The cyprinodont fishes of the Death Valley system of eastern California and southwestern Nevada. Misc. Publ. Mus. Zool. Univ. Mich. 68:1-155. 14 Deacon, J.E. 1979. Endangered and threatened fishes of the West. IN: The endangered species: A symposium. Great Basin Nat. Mem. 3:41-64. 15 Schwartz, A. 1984. Bright future for a desert refugium. Garden May/June:26-29. 16 Deacon, J.E. and T. Baugh. 1985. Interim report on Devils Hole, March - April, 1985. On file with Superintendent, Death Valley National Monument, CA. 5 pp. 17 Deacon, J.E. and T. Baugh. 1985 Summary report on the Hoover Dam Desert Fish Refugium. On file with Bur. Recl., Boulder City, NV. March 1, 1985. 4 pp. References - 1 (DRAFT) - References Species PUPFISH, DEVILS HOLE Species Id ESIS251005 Date 14 MAR 96 18 Baugh, T.M. and J. Deacon. 1984. Management of the Devils Hole pupfish, Cyprinodon diabolis Wales, in the Hoover Dam Pupfish Refugium. On file with Bur. Recl., Boulder City, NV. November 1984. 23 pp. 19 Minckley, C.O. and J. Deacon. 1973. Observations on the reproductive cycle of Cyprinodon diabolis. Copeia 1973(3):610-613. 20 Castro, A. 1983. It's a girl. Page 97. IN: Proceedings of the Desert Fishes Council. P. Pister, ed. 3-9.1-363. 21 Bateman, R.L., A.L. Mindling, and R.L. Naff. 1974. Development and management of ground water in relation to preservation of desert pupfish in Ash Meadows, southern Nevada. Tech. Rept. Ser. H-W, Center for Water Resour. Res. Publ. 17, Univ. Nevada, Reno. 22 Baugh T.M. and J.E. Deacon. 1983. Maintaining the Devil's Hole pupfish, Cyprinodon diabolis Wales in aquaria. J. Aquariculture Aquat. Sci. 3(4):73-75. 23 Beatley, J.C. 1978. Ash Meadows: Nevada's unique oasis in the Mojave Desert. Mentzelia 3:20--24. 24 Dudley, W.W., Jr. and J.D Larson. 1976. Effect of irrigation pumping on desert pupfish habitats in Ash Meadows, Nye Co., Nevada. U.S. Geol. Surv. Prof. Pap. 927:1-52. 25 Walker, G.E. and T.E. Eakin. 1963. Geology and ground water at Amargosa Desert, Nevada-California. Nevada Dept. Conserv. Nat. Res., Water Res. Reconn. Ser. Rept. 14:1-45. 26 Worts, G.F., Jr. 1963. Effect of ground water development on the pool level in Devil's Hole, Death Valley National Monument, Nye Co., Nevada. U.S. Geol. Surv., Water Res. Div., Carson City, NV. 27 pp. ***** REFERENCES FOR N-OCCURRENCE NARRATIVE ONLY ***** 01 Miller, R.R. 1948. The cyprinodont fishes of the Death Valley system of eastern California and southwestern Nevada. Misc. Publ. Mus. Zool. Univ. Mich. 68:1-155. 02 Wales, J.H. 1930. Biometrical studies of some races of cyprinodont fishes from the Death Valley region, with description of Cyprinodon diabolis, n. sp. Copeia 1930:61-70. 03 Williams, J.E. 1977. Observations on the status of the Devil's Hole pupfish in the Hoover Dam Refugum. USDI, Bur. Recl., REC ERC 77-11. 15 pp. 04 Pister, E.P. 1983. Page 2. Proceedings of the Desert Fishes Council. 3-9:1-363. 05 Pister, E.P. 1983. Page 57. Proceedings of the Desert Fishes Council. 3-9:1-363. 06 Pister, E.P. 1983. Pages 57,109,124,209. Proceedings of the Desert Fishes Council. 3-9:1-363. References - 2