(DRAFT) - Taxonomy
                             Species KILLIFISH, PAHRUMP
                                Species Id ESIS251010
                                   Date 14 MAR 96



TAXONOMY

NAME - KILLIFISH, PAHRUMP OTHER COMMON NAMES - KILLIFISH, PAHRUMP; POOLFISH and PAHRUMP ELEMENT CODE - CATEGORY - Fish PHYLUM AND SUBPHYLUM - CHORDATA, CLASS AND SUBCLASS - OSTEICHTHYES, ORDER AND SUBORDER - ATHERINIFORMES, FAMILY AND SUBFAMILY - GOODEIDAE, GENUS AND SUBGENUS - EMPETRICHTHYS, SPECIES AND SSP - LATOS, SCIENTIFIC NAME - EMPETRICHTHYS LATOS AUTHORITY - TAXONOMY REFERENCES - COMMENTS ON TAXONOMY - Pahrump Killifish Empetrichthys latos Miller, 1948 KINGDOM: Animal GROUP: Fish PHYLUM: Chordata CLASS: Osteichthyes ORDER: Atheriniformes FAMILY: Goodeidae This species was originally described by Miller (19) who states that "there are 143 paratypes, U.M.M.Z. No. 140489, 15 to 48 mm long, taken with the holotype at Manse Ranch, and 34 paratypes, U.M.M.Z., 10-50 mm, collected by R.R. Miller and Alex J. Calhoun on July 16, 1938, from the outlet of the main spring pool" at Manse Ranch. These and additional specimens are maintained at the University of Michigan Museum of Zoology. The species was originally not recognized as distinct and was therefore included by Gilbert (12) in his description of E. merriami. According to Miller (19), this species is relatively slender "with a gently sloping to convex predorsal profile, a relativeely short and slender head, a comparatively broad mouth, a weak mandible, and usually 31 or 32 scales in the lateral series." The sides are marked by a narrow axial streak and there are 12-13 anal rays. "Empetrichthys latos differs from E. merriami principally in Taxonomy - 1 (DRAFT) - Taxonomy Species KILLIFISH, PAHRUMP Species Id ESIS251010 Date 14 MAR 96 mouth structure, body shape and color pattern." In E. latos the mouth is wider and the bones of the premaxillary and mandible are weaker and less firmly connected. The head of E. latos is not as deep and the body not as thick as in E. merriami. As is true of a few other isolated desert fishes, E. latos has lost its pelvic fin. The species is illustrated in Soltz and Naiman (23). Three subspecies of E. latos are recognized: E. latos latos, E. latos concavus, and E. latos pahrump. Only E. latos latos (23) is known extant. The common name Pahrump poolfish also refers to this species. While specific and subspecific definitions of E. latos latos Miller have not changed since they were described (19), placement of the genus in higher taxonomic categories has been an unsolved problem for years. When described, Empetrichthys were placed in the Cyprinodontidae family. Jordan and Evermann (13) included the genus within their expanded family Poeciliidae. Eigenmann (11) placed Empetrichthys in the distinct family Empetrichyidae. Myers (18) returned the genus to the Cyprinodontidae where it remained until 1981 when Parenti (21) placed the genus into the family Goodeidae. Taxonomy - 2
                                  (DRAFT) - Status
                             Species KILLIFISH, PAHRUMP
                                Species Id ESIS251010
                                   Date 14 MAR 96



STATUS

Coded Status E: Federal Endangered COMMENTS ON STATUS - U.S. STATUSES AND LAWS: The Pahrump killifish (Empetrichthys latos) has been designated an Endangered species pursuant to the Endangered Species Act of 1973 (50 CFR 17.11; P.L. 93-205, 87 Stat. 884; 16 U.S.C. 1531-1540), as amended. The species has this status wherever found including the State of Nevada. This species is protected by the Lacey Act (P.L. 97-79, as amended; 16 U.S.C. 3371 et seq.) which makes it unlawful to import, export, transport, sell, receive, acquire, or purchase any wild animal (alive or dead including parts, products, eggs, or offspring): (1) in interstate or foreign commerce if taken, possessed, transported or sold in violation of any State law or regulation; or (2) if taken or possessed in violation of any U.S. law, treaty, or regulation or in violation of Indian tribal law. It is also unlawful to possess any wild animal (alive or dead including parts, products, eggs, and offspring) within the U.S. territorial or special maritime jurisdiction (as defined in 18 U.S.C. 7) that is taken, possessed, transported, or sold in violation of any State law or regulation, foreign law, or Indian tribal law. RESPONSIBLE FEDERAL AGENCIES: USFWS -Responsible for the management/recovery, listing, and law enforcement/protection of this species. BLM -Responsible for the law enforcement/protection of this species with applicable State and Federal laws on public land under their control (43 CFR 4140). Also responsible for management/recovery on Bureau of Land Management lands. All Federal agencies have responsibility to ensure that any action authorized, funded, or carried out by that agency is not likely to jeopardize the continued existence of the species or result in the destruction or adverse modification of Critical Habitat (50 CFR 402), and to utilize their authorities to carry out programs for the conservation of the species. STATE STATUSES AND LAWS: STATE: Nevada Status - 1 (DRAFT) - Status Species KILLIFISH, PAHRUMP Species Id ESIS251010 Date 14 MAR 96 DESIGNATED STATUS: Endangered ADMINISTRATIVE AGENCY: Nevada Department of Wildlife STATE STATUTE: NV Rev. Stat. 501.181, 501.351, 503.584, 503.585, 503.587 and 503.589. INTERNATIONAL STATUSES, TREATIES, AND AGREEMENTS: The Pahrump killifish is listed as endangered in the IUCN Red Data Book, vol. 4, 1977. ECONOMIC STATUSES: None. 67/03/11:32 FR 04001 - Listed as Endangered (1966 Act). 70/08/25:35 FR 13519/13520 - Proposed List/Endangered (1969 Act). 70/10/13:35 FR 16047/16048 - Listed as Endangered. 79/05/21:44 FR 29566/29577 - Five year review. 85/07/22:50 FR 29901/29909 - Five year review. Status - 2
     

HABITAT ASSOCIATIONS

HABITAT - AQUATIC INLAND AQUATIC LAND USE - Herbaceous Rangeland Shrub and Brush Rangeland Reservoirs NATIONAL WETLAND INVENTORY CODES NWI NWICLS NWIMOD NWISPEC Lacustrine, littoral AB2 Lacustrine, littoral AB1 COMMENTS ON HABITAT ASSOCIATIONS - The Pahrump killifish no longer exists in the type of locality at Manse Ranch, Nye Co., Nevada, having been extirpated from the site in the mid-1970's. Two other natural habitats in Pahrump Valley, Nevada, were destroyed in the mid-1950's. This species is now maintained in three refugia, Corn Creek Springs, Spring Mountain Ranch State Park, and Shoshone Ponds. All three areas are bordered by natural desert vegetation and are protected from excessive public disturbance. Corn Creek Springs on the Desert National Wildlife Range is located about 40 miles north of Las Vegas, Nevada. The aquatic system is composed of a concrete trough about 100 meters long from the spring sources to the first and largest of three ponds. Submergent and emergent vegetation in the trough is restricted to some willow roots and a small growth of Myrophyllum sp. about midway along the channel. The first pond is roughly 80 meters long by about 25 meters wide and terminates in a flume which drains into a stream about 150 meters long. The stream subsequently drains into a middle pond which is roughly 8 meters in circumference. The middle pond drains directly into a third and final pond which is roughly 25 meters in circumference. The northwest bank of this pond is heavily colonized by Typha. About 50% of this pond is, however, free of emergent vegetation. Submergent vegetation occupies the area not colonized by emergents and is composed mainly of Potamogeton. Water temperatures at Corn creek Springs were taken on Dec. 21, 1983 (01) and were as follows: Location: Temperature (Celsius): Spring Outflow 25 Upper Pond Inflow 25 Upper Pond Outflow 19.4 Midstream 19.4 Middle Pond Inflow 18.9 Middle Pond Outflow 15.6 Lower Pond Inflow 13.9 The aquatic site at Spring Mountain Ranch State Park is composed of an irrigation impoundment of about 21 acre feet. The east bank is colonized by Typha along about one-half of its approximate 0.2 mile length. The west bank is heavily colonized by Typha to an average estimated distance of about 30 feet from shore. Each February or March, the water in the impoundment is lowered and the dry Typha is burned. Chara covers about 70% of the submerged rocky-sandy substrate. At Spring Mountain Ranch the inflowing water, as well as Habitat Associations - 1 the dam, must be maintained. The aquatic site used by this species at Shoshone Ponds is composed of three of the five ponds extant at the site. Shoshone North Pond is the only pond which has had extensive investigation (08). This man-made pond is about 7 x 9 meters with a depth of about 1.2 meters. Surface dissolved oxygen varies from 9.6-12.0 ppm between June and July. Surface water temperature varies from 26.0-27.0 deg Celsius, pH varies from 8.5-9.35, hardness varies from 52-53, and alkalinity varies from 51-55. Mark Barber (25) of the Bureau of Land Management reports the following chemical and physical characteristics of the water at Shoshone North Pond: pH 7.5 Turbidity (JTU) 3.4 Dissolved Solids 97.0 Nitrate-N mg/l 0.06 Chloride mg/l 4.2 Calcium mg/l 14.0 Magnesium 0.35 Maganese 1.5 Potassium 10.0 Sodium 7.0 Sulfate 70.0 Selby (22) determined critical thermal limits for Pahrump killifish of less than 1.5 deg Celsius to 40 deg Celsius. Acclimation temperatures influence these limits so that cold-acclimated fish show a critical thermal maximum at 30 deg Celsius and a critical thermal minimum below 1.5 deg Celsius, while comparable limits for warm-acclimated fish are 40 deg and 6.9 deg Celsius, critical thermal maximum and minimums respectively. Limits of temperature tolerance are substantial and in natural waters, are unlikely to be exceeded. Experimental results indicated that the species does not have a preferred temperature and does not appear to behaviorally thermoregulate. This conclusion is supported by catch data from Corn Creek Spring. Selby (22) also examined oxygen tolerance of Pahrump killifish and noted that stress was not apparent until oxygen levels fell to near 1 ppm at 22-25 deg Celsius. Distribution of the population in Corn Creek Spring, however, suggests a slight preference for waters containing a high concentration of dissolved oxygen. Habitat Associations - 2
                                (DRAFT) - Food Habits
                             Species KILLIFISH, PAHRUMP
                                Species Id ESIS251010
                                   Date 14 MAR 96



FOOD HABITS

TROPHIC LEVEL - OMNIVORE LIFESTAGE FOOD FOOD PART General Phytoplankton General Zooplankton General Arthropods General Molluscs General Detritus Food Habits - 1
                         (DRAFT) - Environment Associations
                             Species KILLIFISH, PAHRUMP
                                Species Id ESIS251010
                                   Date 14 MAR 96



ENVIRONMENTAL ASSOCIATIONS

G = General A = Adult LIM = Limiting RA = Resting Adult J = Juvenile FA = Feeding Adult RJ = Resting Juvenile BA = Breeding Adult FJ = Feeding Juvenile P = Pupae L = Larvae E = Egg RL = Resting Larvae FL = Feeding Larvae
LIFESTAGE ENVIRONMENTAL ASSOCIATIONS G Human Association: Public residential parks G Aquatic Features: Springs [flowing] G Human Association: Farm ponds Environment Associations - 1
                               (DRAFT) - Life History
                             Species KILLIFISH, PAHRUMP
                                Species Id ESIS251010
                                   Date 14 MAR 96



LIFE HISTORY

FOOD HABITS: Deacon (08) presents the following evaluation of the food habits of adult Empetrichthys latos latos at Manse Ranch for the period 1961-1963: (%) Frequency Mean Number Mean (%) Food Item: of Occurrence: per Fish: Volume: Debris 78.3 55.7 Insect parts 38.3 1.13 10.69 Sand 35.8 9.13 Insect Larvae 15.0 1.54 8.0 Eggs 11.1 0.20 3.13 Snails 14.1 1.98 3.05 Plant 5.0 2.2 Baetidae 5.0 1.67 1.60 Coleoptera 6.6 0.37 1.53 Insecta 9.1 0.09 1.13 Physa 5.0 0.55 1.25 Ephemeroptera 2.5 0.54 0.59 Hydrobioid 5.9 0.31 0.33 Cladocera 3.3 4.00 0.10 Ostracod 3.3 0.22 0.09 In summary, debris constitutes 55.7% of the diet, with the following items appearing in lesser amounts in the diet: Insect parts 10.7%; Sand 9.1%; Insect larvae 8.0%; Other insecta 4.8%; Snails 4.6%; Eggs 3.1%; and Plants 2.2%. Juveniles and young probably depend on zooplankton algae and debris as their primary food sources. HOME RANGE/TERRITORY: The social behavior of this species has not been investigated. PERIODICITY: Deacon and Minckley (10) report that the Pahrump killifish exhibit daily activity cycles similar to those shown for other fishes. They are active during the day with mid-day depression of activity and more inactive at night. Selby (22) suggested that low oxygen concentrations in dense vegetation during hours of darkness may force the species to move into more open waters where oxygen concentrations remain above 1 ppm. This response was especially notable at colder temperatures when fish appeared to move about only at night when apparently forced out of the vegetation by lower oxygen concentration. At temperatures above 20 deg Celsius the species was primarily diurnal. MIGRATION PATTERNS: Isolated in desert springs, this is a non-migratory species. COVER/SHELTER REQUIREMENTS: Selby (22) indicates that the Pahrump killifish moves away from Life History - 1 (DRAFT) - Life History Species KILLIFISH, PAHRUMP Species Id ESIS251010 Date 14 MAR 96 areas of extremely low (< 1.0 ppm) oxygen concentration which may occur especially at night in areas of dense aquatic vegetation. Deacon (05) and Baugh (02) show that fry prefer densely vegetated shallow water at Springs Mountain Ranch State Park. Vegetation at that location consisted primarily of cattails (Typha) and Chara. REPRODUCTIVE SITE REQUIREMENTS: Pahrump killifish have been spawned successfully in aquaria depositing eggs on "Living World Spawning Grass" at a temperature of 23 deg Celcius (16) (not a product endorsement, but given for informational purposes). In nature, they may deposit eggs on aquatic vegetation, but no detailed observations have been made. Fry were observed in abundance in shallow, densely-vegetated areas at Spring Mountain Ranch State Park on 6-7 June, 1984, at a water temperature of 16.4 deg Celsius (05). REPRODUCTIVE CHARACTERISTICS: Spawning of Pahrump killifish in constant temperature natural springs probably can occur throughout the year, as is common with desert spring fishes (10). In Manse Spring, the peak of the reproductive season probably was the spring of the year (February - April) (16). Spawning did occur in the variable thermal environment at Spring Mountain Ranch State Park during late May to early June at water temperatures that were probably near 15 deg Celsius but with diet variation (05). In laboratory aquaria at Univ. of Nevada, Las Vegas, eleven female killifish produced an average of 14 eggs, each over a 24-hour spawning period. One attempt suggests that a single female may not be ready to spawn again within a month of her first spawning. It also appears that females of about 45 mm TL and above are mature and capable of producing a full complement of eggs. Females of smaller sizes are less frequently capable of spawning (16). Eggs hatch in 7-9 days at 25 deg Celsius. Larvae are about 6-11 mm TL at hatching. Young grow to an average size of about 20 mm TL in 112 days, but exhibit increasing size variation with time. Females probably grow faster than males (16). PARENTAL CARE: Pahrump killifish do not protect eggs or fry. POPULATION BIOLOGY: Pahrump killifish maintain their populations in desert springs containing no other fish species. They survive and reproduce well in these stable habitats with a diversity of plant and invertabrate species (08,23). In the natural habitat, Manse Spring, introduction of goldfish was soon followed by a rapid decline in the killifish population (03,09). At Corn Creek a similar population crash followed re-establishment of the mosquitofish population (03,15). The Manse Spring population recovered and appeared to maintain a good population along with goldfish. The indication was that the species may not have recovered from the impacts of the mosquitofish without management intervention. Interaction with introduced fishes thus appears to be a Life History - 2 (DRAFT) - Life History Species KILLIFISH, PAHRUMP Species Id ESIS251010 Date 14 MAR 96 serious threat to a Pahrump killifish population. Sex ratios have varied from 1 male to 1.2 females at the middle Corn Creek pond to 1:7.9 at the lower pond at Corn Creek. Females outnumber males in every population (04). This species, in the undisturbed desert spring habitats into which it has been transplanted, is maintaining excellent populations, under appropriate and continuous management. At Corn Creek, encroachment of emergent aquatic vegetation must be controlled (04). At Shoshone Ponds, the water system must be maintained. At Spring Mountain Ranch the inflowing water, as well as the dam, must be maintained. At all locations, introduced fishes must be kept out or, if introduced, they must be removed, SPECIES INTERRELATIONSHIPS: Species interrelationships were disccussed above under Population biology. OTHER LIFE HISTORY DESCRIPTORS: None. Life History - 3
                           (DRAFT) - Management Practices
                             Species KILLIFISH, PAHRUMP
                                Species Id ESIS251010
                                   Date 14 MAR 96



MANAGEMENT PRACTICES

RESULT MANAGEMENT PRACTICE Beneficial Regulating Water Temperature Beneficial Maintaining/Controlling Water Flow Beneficial Developing/maintaining/protecting wetlands Beneficial Controlling water levels Beneficial Land Acquisition Beneficial Water Right Acquisition Beneficial Reforestation Beneficial Controlling/Removing Nonnative Vegetation Beneficial Controlling/Removing Native Vegetation Beneficial Transplanting wild animals Beneficial Restricting Poaching Beneficial Controlling/Removing Native Vertebrates Beneficial Controlling/Removing Exotic Vertebrates Adverse Existing Adverse Groundwater drawdown Existing Groundwater drawdown Adverse Irrigating Existing Irrigating Adverse Exotic/Feral/Introducted Species Existing Exotic/Feral/Introducted Species Adverse Existing COMMENTS ON MANAGEMENT PRACTICES - Habitat destruction is regarded as the primary reason for the Endangered status of the Pahrump killifish. Excess pumping of groundwater for agricultural irrigation is seen as the primary cause of habitat destruction (03,20,23). Development of groundwater for irrigation in Pahrump Valley began in the early 1900's. Flow of the three major springs in the valley (each containing a distinct subspecies of Pahrump killifish) declined as groundwater pumping increased. Two of the three springs ceased to flow in the mid-1950's and the populations of Pahrump killifish became extinct. The third spring, Manse Spring, ceased flow in 1975 (23). In August, 1971, 29 Pahrump killifish from Manse Spring were transplanted into Corn Creek Spring in the Las Vegas Valley (03). With continued management, this population has been sustained since that time (03,04, 05,06,07). On March 9, 1972, 16 pahrump killifish (from Corn Creek or Manse Spring, the exact collection site is not clear) were introduced into one of the nearly completed ponds at Shoshone ponds, White Pine Co., Nevada, about 50 km S.E. of Ely, Nevada, in the NE 1/4, Sec. 2, T12N, R57E. Eight of these fish were transferred to the south pond on 26 September, 1972, but the fish were not observed in either pond after Spring 1974 (reasons for the fish disappearance within these two ponds are uncertain, possibilities range from too low of numbers to adverse conditions) (17). On August 31, 1976, 50 Pahrump killifish Management Practices - 1 (DRAFT) - Management Practices Species KILLIFISH, PAHRUMP Species Id ESIS251010 Date 14 MAR 96 were transferred from Corn Creek Spring into Shoshone North Pond (15). Subsequently, the species was transferred into two other ponds in the Shoshone Ponds complex. It presently maintains reproducing populations in 3 of the 5 Shoshone Ponds. On June 9, 1983, 426 Pahrump killifish from the Corn Creek Spring were introduced into the irrigation reservoir at Spring Mountain Ranch State Park, Clark Co., Nevada (05). The population reproduced and maintained itself through at least May, 1985 (02). All native habitats of the Pahrump killifish have been destroyed. Transplanted populations of the species persist at Corn Creek Spring, Shoshone Ponds and Spring Mountain Ranch State Park. All of these habitats are likely to require management intervention to maintain healthy populations. Mosquitofish (an exotic) at Corn Creek were the apparent cause of an extremely depressed populations of Pahrump killifish on at least two occasions. The mosquitofish have been eliminated at present, but could be reintroduced at any time. An artesian well supplies water to the Shoshone Ponds. It must be maintained to insure that the water supply remains reliable. At Spring Mountain Ranch State Park reintroduction of other fishes must be prevented. In addition, the winter cooling of water renders the killifish inactive for extended periods and presumably could, under extreme weather conditions, result in winter mortality. APPROVED PLAN: U.S. Fish and Wildlife Service. 1980. Pahrump Killifish Recovery Plan. U.S. Fish and Wildl. Ser., Portland, OR, 38 pp. The primary objective of the recovery plan for the Pahrump killifish is the establisment of three sub-populations. If the three sub-populations are maintained with a minimum of 300 adults for three years in habitats free of immediate and potential threats, the species may be considered for reclassification to Threatened. If the criteria for reclassifying can be maintained for an additional three years, the species may be considered for delisting. The recovery activities necessary to acheive the objective are: 1. Preserve and protect transplanted populations and habitats by; a. eliminating exotic fishes, b. controlling bullfrogs and turtles, c. controlling birds, d. controlling vegetation (native and non-native), e. securing the acquifer, f. rehabilitating pond habitat (through land acquisition and/or securing water rights). Any controllable factor which reduces water volume should receive attention, therefore removal of undesireable emergent vegetation is necessary to prevent excessive evapotranspiration. Other items to be attended to are: (1) constructing a spring in the lower pond at Shoshone Ponds, to prevent encroachment of emergent vegetation, (2) protecting springs, by covering spring heads and removing excessive vegetation to aid in conserving water. (3) constructing concrete ditches, to reduce seepage and Management Practices - 2 (DRAFT) - Management Practices Species KILLIFISH, PAHRUMP Species Id ESIS251010 Date 14 MAR 96 enhance exotic species control (all parts of wetland management and modification); g. monitoring populations, h. controlling water (both maintaining flow and water table), i. planting of trees along shorelines, this will provide shade, control water temperature, and loss of water by evapotranspiration, and j. enforcing laws and regulations (for habitat and species protection - i.e. poaching, prevention of exotic introductions) 2. Establish and protect viable, self-sustaining populations in new or restored habitas which will invlove; a. removing exotic species, predatory species, b. controlling native and non-native vegetation, c. acquiring or assuring property and water rights, d. enhance habitat features, e. preparing habitat management plan, f. conducting transplants and monitoring results; 3. Apply results of ecological studies to management of species and habitats, and delineate essential habitats; 4. Implement public information and education program through publications, audio-visual programs, news releases to media, and informational signs. Management Practices - 3
                                   (DRAFT) - References
                                Species KILLIFISH, PAHRUMP
                                  Species Id ESIS251010
                                      Date 14 MAR 96



     

References

***** REFERENCES FOR ALL NARRATIVES EXCEPT N-OCCURRENCE ***** 01 Baugh, T.M. and J.E. Deacon. 1984. Pahrump killifish, iterim report. Rept. to: Nev. Dept. Wildl., 17 Jan., 1984. 14 pp. 02 Baugh, T.M., J. Pedretti and J.E. Deacon. 1985. Status of the Pahrump poolfish Empetrichthys latos latos in the irrigation impoundment at Spring Mountain Ranch State Park. Rept. to: Nev. Dept. of Wildl. 03 Deacon, J.E. 1979. Endangered and threatened fishes of the west. Gt. Basin Nat. Mem. 3:41-64. 04 Deacon, J.E. 1984. Survey of Empetrichythys latos latos at Corn Creek, 6/11-15/84. Rept. to: Nev. Dept. of Wildl. 27 June, 1984. 9 pp. 05 Deacon, J.E. 1984. Status of Empetrichthys latos latos in the irrigation impoundment at Spring Mountain Ranch State Park. Rept. to: Nev. Dept. of Wildl. 21 June, 1984. 7 pp. 06 Deacon, J.E. 1984. Status of Pahrump poolfish, E. latos latos in Spring Mountain Ranch State Park. Rept. to: Nev. Dept. of Wildl. 24 Aug., 1984. 7 pp. 07 Deacon, J.E. 1984. Recovery efforts for the Pahrump killifish: Shoshone North Pond. Rept. to: Nev. Dept. of Wildl., 24 Aug., 1984. 26 pp. 08 Deacon, J.E., T.B. Hardy, J. Landye, J. Pollard, W. Taylor, and P. Greger. 1980. Environmental analysis of four aquatic habitats in east-central Nevada, June-July, 1980. Interim Summ. Rep. to HDR Sci., Cont. No. HDR/RPA15. 09 Deacon, J.E., C. Hubbs, and B.J. Zahuranec. 1964. Some effects of introduced fishes on the native fish fauna of southern Nevada. Copeia 1964:384-388. 10 Deacon, J.E. and W.L. Minckley. 1974. Desert fishes. Pp. 385-487. In: Desert Biology. G.W. Brown, Jr., ed., Vol. 2. Academic Press, N.Y. 11 Eigenmann, C.H. 1920. On the genera Orestias and Empetrichthys. Copeia, No. 89:103-106. 12 Gilbert, C.M. 1893. Report on the fishes of the Death Valley expedition in southern California and Nevada in 1891, with descriptions of new species. N. Am. Fauna, No. 7, Pt. II:229-234. 13 Jordan, D.S. and B.W. Evermann. 1896. The fishes of North and Middle America. Bull. U.S. Nat. Mus. 47, Pt. I:1-1240. 14 Jordan, D.S., B.W. Evermann, and H.W. Clark. 1930. Check list of the fishes and fishlike vertebrates of North and Middle American, North of the Northern boundary of Venezuela and Columbia. Rept. U.S. Comm. Fish. 1928, Pt. 2:1-670. 15 Kobetich, G. 1983. Nevada Interior Basins. Pp. 289-290. In: E.P. Pister (ed.) Proc. Desert Fishes Council 3-9. 363 pp. 16 Fitzpatrick, P., T.M. Baugh, and Deacon. 1985. Age and growth of the Pahrump poolfish, Empetrichtys latos latos in the laboratory. Rept. to: Nev. Dept. of Wildl. 1985. 17 Logan, Wayne. 1983. Progress report on Shoshone Ponds. Pp. 138- 139. In: E.P. Pister (ed.) Proc. Des. Fishes Coun. 3-9. 363 pp. 18 Myers, G.S. 1931. The primary groups of oviparous cyprinodont References - 1 (DRAFT) - References Species KILLIFISH, PAHRUMP Species Id ESIS251010 Date 14 MAR 96 fishes. Stanford Univ. Publ. Univ. Ser., Biol. Sci. 6(3):1-14. 19 Miller, R.R. 1948. The cyprinodont fishes of the Death Valley system of eastern California and southwestern Nevada. Misc. Publ. Mus. Zool. Univ. Mich. 68:1-155. 20 Minckley, W.L. and J.E. Deacon. 1968. Southwestern fishes and enigma of "endangered species". Science 159:1424-1432. 21 Parenti, Lynne. 1984. A phylogenetic and biogeographic analysis of Cyprinodontiform fishes (Teleostei, Antherinomorpha). Bull. Am. Mus. Nat. Hist. 168(4):335-557. 22 Selby, D.A. 1977. Thermal ecology of the Pahrump killifish, Empetrichthys latos latos Miller. M.S. thesis. Univ. Nev. Las Vegas. 55 pp. 23 Soltz, D.L. and R.J. Naimon. 1978. The natural history of native fishes in the Death Valley system. Nat. Hist. Mus. of Los Angeles County and Death Valley Nat. Hist. Assoc., Science Series. No. 30, 76 pp. 24 U.S. Fish and Wildlife Service. 1980. Recovery Plan: Pahrump killifish. U.S. Fish and Wildl. Serv., Portland, OR. 29 pp. 25 Barber, Mark. 1985. Pers. comm. Bureau of Land Management, Ely, Nevada. ***** REFERENCES FOR N-OCCURRENCE NARRATIVE ONLY ***** 0$ Baugh, T.M., J. Pedretti, and J.E. Deacon. 1985. Status of the Pahrump poolfish Empetrichthys latos latos in the irrigation impoundment at Spring Mountain Ranch State Park. Report to: Nev. Dept. of Wildl. 02 Deacon, J.E. 1984. Pahrump poolfish, Cont. E-1-1, Job 3, Inter. Rept., 6/7/84. Rept. to: Nev. Dept. of Wildl. 21 June, 1984. 9 pp. 03 Deacon, J.E. 1984. Interim Rept., Project E-1-1, Job 3, Corn Creek, 6/18/84. Rept. to: Nev. Dept. of Wildl. 27 June, 1984. 7 pp. 04 Deacon, J.E. 1984. Recovery efforts for the Pahrump killifish. Rept. to: Nev. Dept. of Wildl. 24 Aug., 1984. 7 pp. 05 Fitzpatrick, P., T.M. Baugh, and J.E. Deacon. 1985. Age and growth of the Pahrump poolfish, Empetrichthys latos latos in the laboratory. Rept. to: Nev. Dept. of Wildl. 06 Miller, R.R. 1948. The cyprinodont fishes of the Death Valley system of eastern California and southwestern Nevada. Misc. Publ. Mus. Zool. Univ. Mich. 68:1-155. 07 Minckley, W.L. and J.E. Deacon. 1968. Southwestern fishes and enigma of "endangered species". Science 159:1424-1432. 08 Soltz, D.L. and R.J. Naimon. 1978. The natural history of native fishes in the Death Valley system. Nat. Hist. Mus. of Los Angeles County and Death Valley Nat. Hist. Assoc., Science Series. No. 30, 76 pp. References - 2