(DRAFT) - Taxonomy
                              Species CHUB, TUI, OWENS
                                Species Id ESIS251018
                                   Date 14 MAR 96



TAXONOMY

NAME - CHUB, TUI, OWENS OTHER COMMON NAMES - CHUB, TUI, OWENS; CHUB and TUI ELEMENT CODE - CATEGORY - Fish PHYLUM AND SUBPHYLUM - CHORDATA, CLASS AND SUBCLASS - OSTEICHTHYES, ORDER AND SUBORDER - CYPRINIFORMES, FAMILY AND SUBFAMILY - CYPRINIDAE, GENUS AND SUBGENUS - GILA, SPECIES AND SSP - BICOLOR, SNYDERI SCIENTIFIC NAME - GILA BICOLOR SNYDERI AUTHORITY - TAXONOMY REFERENCES - COMMENTS ON TAXONOMY - Owens Tui Chub Gila bicolor snyderi Miller, 1973 KINGDOM: Animal GROUP: Fish PHYLUM: Chordata CLASS: Osteichthyes ORDER: Cypriniformes FAMILY: Cyprinidae General Description: a representative of subgenus Siphateles. Differs from other subspecies by having: 1) pharyngeal arches with a strong shield at the posterior end of the anterior limb, and 2) scales with a weak or no basal shield and with lateral and apical radii, the total number of radii varying from 13 to 29, 3) the dentary deep below the subvertical ascending process with gnathic ramus strong and evenly curved and the thin, elevated ridge of the dentary flared away from the median, 4) usually from 10 to 14 gill rakers, 7 anal rays, and from 52 to 58 lateral line scales. In life the color is dusky olive above and whitish below, with blue and gold reflections along the side. There is considerable gold on the side of the head, often strongest along the margin of the Taxonomy - 1 (DRAFT) - Taxonomy Species CHUB, TUI, OWENS Species Id ESIS251018 Date 14 MAR 96 preopercle (03). This fish was incorrectly regarded as Rutilus symmetricus by Gilbert in 1893 (02). It was regarded as Siphateles obesus by Snyder in 1917 (04), but Siphateles was merged into the genus Gila in 1964 by Bailey and Uyeno (01). Siphateles is now regarded as a subgenus of Gila by Miller (03). The subspecies snyderi was officially recognized in 1973 by Miller (03). Type specimens are located at the Museum of Zoology, Univ. of Michigan, Ann Arbor (03). Taxonomy - 2
                                  (DRAFT) - Status
                              Species CHUB, TUI, OWENS
                                Species Id ESIS251018
                                   Date 14 MAR 96



STATUS

Coded Status E: Federal Endangered COMMENTS ON STATUS - U.S. STATUSES AND LAWS: The Owens tui chub (Gila bicolor snyderi) has been designated as Endangered pursuant to the Endangered Species Act of 1973 (50 CFR 17.11; P.L. 93-205, 87 Stat. 884; 16 U.S.C. 1531-1540), as amended. The subspecies has this status wherever found including the state of CA. Critical Habitat has been designated for this species in portions of Hot Creek (T35, R28E, SW 1/4, Sec. 35) and Owens River (T4S, R3OE, Sec. 19 - 25 and 36) in Mono County, CA. Known constituent elements include high quality, cool water with adequate cover of rocks, undercut banks or aquatic vegetation and a sufficient insect food base (50 CFR 17.95(e)). This subspecies is protected by the Lacey Act (P.L. 97-79, as amended; 16 U.S.C. 3371 et seq.) which makes it unlawful to import, export, transport, sell, receive, acquire, or purchase any wild animal (alive or dead including parts, products, eggs, or offspring): (1) in interstate or foreign commerce if taken, possessed, transported or sold in violation of any State law or regulation, or foreign law; or (2) if taken or possessed in violation of any U.S. law, treaty, or regulation or in violation of Indian tribal law. It is also unlawful to possess any wild animal (alive or dead including parts, products, eggs, and offspring) within the U.S. territorial or special maritime jurisdiction (as defined in 18 U.S.C. 7) that is taken, possessed, transported, or sold in violation of any State law or regulation, foreign law, or Indian tribal law. RESPONSIBLE FEDERAL AGENCIES: USFWS -Responsible for the management/recovery, listing, and law enforcement/protection of this species. USFS -Responsible for the law enforcement/protection of this species with applicable State and Federal laws on public lands under their control. Also responsible for management/recovery on Forest Service lands. The Forest Service is responsible for integrating management, protection, and conservation of Federally listed species into the Forest Planning process (36 CFR 219.19 and 219.20). Management practices that would cause detrimental changes in water temperature or composition, water course blockage, or sediment deposits within 100 feet of the edges of perennial Status - 1 (DRAFT) - Status Species CHUB, TUI, OWENS Species Id ESIS251018 Date 14 MAR 96 streams, lakes or other bodies of water are prohibited (36 CFR 219.27(e)). USFS's management of surrounding areas directly impacts the critical habitat of this species. All Federal agencies have responsibility to ensure that any action authorized, funded, or carried out by that agency is not likely to jeopardize the continued existence of the species or result in the destruction or adverse modification of Critical Habitat (50 CFR 402), and to utilize their authorities to carry out programs for the conservation of the species. STATE STATUSES AND LAWS: STATE: California DESIGNATED STATUS: Endangered ADMINISTRATIVE AGENCY: California Dept. of Fish and Game STATE STATUTE: California Endangered Species Act, Fish and Game Code, Chapter 1.5, Article 1, Section 2050. INTERNATIONAL STATUSES, TREATIES, AND AGREEMENTS: The Owens tui chub is listed as Endangered in the IUCN Red Data Book, Vol. 4, 1977. ECONOMIC STATUSES: None. 82/12/30:47 FR 58454/58460 - Status review 83/06/14:48 FR 27273/27274 - Notice of findings 83/10/25:48 FR 49244/ - Reasons for no environment assessment 84/03/23:49 FR 10959/10962 - Proposed rule 85/08/05:50 FR 31592/31597 - Final rule, Endangered w/ Crit. Hab. Status - 2
     

HABITAT ASSOCIATIONS

HABITAT - AQUATIC INLAND AQUATIC LAND USE - Shrub and Brush Rangeland Streams and Canals Reservoirs NATIONAL WETLAND INVENTORY CODES NWI NWICLS NWIMOD NWISPEC Riverine, lower perennial AB2 Riverine, lower perennial UB3 Riverine, lower perennial Riverine, upper perennial AB2 Lacustrine, limnetic UB COMMENTS ON HABITAT ASSOCIATIONS - The Owens tui chub is associated with streams that have slow current, mud bottoms, and abundant submerged vegetation. Known constitutent elements include high quality, cool water with adequate cover in the form of rocks, undercut banks, or aquatic vegetation, and a sufficient insect food base (05). It is likely, when one considers the past extensive distribution of Gila bicolor snyderi, that this species may have been found in association with a number of different habitat types. Due to predation by introduced exotics, this broad distribution has been restricted to the slower and more protective waters of the Owens River. This includes the submerged vegetation which is used by the tui chub for protection from predator species (20). Kimsey (14) reports that tui chubs in Eagle Lake, California spawn in about three feet of water in temperatures of about 55 to 60 degrees Farenheit in aquatic plant beds of Mirophyllum, Ceratophyllum, and Potamogeton. In laboratory experiments he found that eggs attached to the leaves or stems of these plants hatched while those that fell to the substrate failed to hatch. Currently surrounding habitat is sage brush. One location for the species is below the Long Valley Dam (Long Valley Reservoir also known as Crowley Lake) in Mono County, CA. The Owens Basin consists of three valleys: Long Valley and Adobe Valley in the north, which drain into Owens Valley to the south. During pluvial times, 10,000 to 20,000 years ago, these valleys were dominated by large lakes and were, in general, well-watered (Hubbs and Miller, 1948). In the more arid present climate, the Owens Basin still contains a variety of springs, lakes and flowing water habitats. Many of these habitats, with the exception of thermal springs and high elevation lakes, were at one time inhabited by the Owens tui chub. Much of the natural habitat has been modified for irrigation purposes, impounded to create reservoirs, or dewatered to provide for the needs of Los Angeles. Habitat Associations - 1
                                (DRAFT) - Food Habits
                              Species CHUB, TUI, OWENS
                                Species Id ESIS251018
                                   Date 14 MAR 96



FOOD HABITS

TROPHIC LEVEL - OMNIVORE LIFESTAGE FOOD FOOD PART General Detritus General Bacteria General Phytoplankton General Diatoms General Algae General Zooplankton General Arthropods Food Habits - 1
                         (DRAFT) - Environment Associations
                              Species CHUB, TUI, OWENS
                                Species Id ESIS251018
                                   Date 14 MAR 96



ENVIRONMENTAL ASSOCIATIONS

G = General A = Adult LIM = Limiting RA = Resting Adult J = Juvenile FA = Feeding Adult RJ = Resting Juvenile BA = Breeding Adult FJ = Feeding Juvenile P = Pupae L = Larvae E = Egg RL = Resting Larvae FL = Feeding Larvae
LIFESTAGE ENVIRONMENTAL ASSOCIATIONS G G Aquatic Features: Springs [flowing] Environment Associations - 1
                               (DRAFT) - Life History
                              Species CHUB, TUI, OWENS
                                Species Id ESIS251018
                                   Date 14 MAR 96



LIFE HISTORY

OOD HABITS: Little is known about the food habits of Gila bicolor snyderi except by comparison with closely related subspecies such as Gila bicolor obesus and other fish of this genus. La Rivers (06) reports that adult lacustrine Gila bicolor in Pyramid Lake, Nevada, are omnivorous feeding on algae covered rocks hosting crustaceans and that stomach contents of these fish contain diatoms, micro-crustacea, hydrocarina, and lendipedid larvae. Larval Pyramid Lake fish are reported to also be opportunistic feeders (07) consuming the cladocerans, Moina hutchinsoni, Diaphanosoma leuchtenbergianum, and Alona costata, the copepods - Cyclops vernalis, and Diaptomus sicilis, and other zooplankters including Eucypris sp., Branchionus sp., Daphina schodleri, as well as copepod nauplii. Cooper (19) found that tui chub from Walker Lake, Nevada, consumed mostly zooplankton (97.4% by occurrence and 94.4% by composition) if feeding in pelagic water but significantly more benthic material when feeding in littoral regions. Galat and Vucinich (07) found food partitioning between the two sympatric species of Gila in Pyramid Lake in that chironomid consumption for young of the year Gila bicolor obesa was 41% of the stomach contents for fish from 26-50 mm long and up to 62% for fish 51-75 mm long while 26-50 mm long G. b. pectinifer consumed 93 pct. zooplankton and fish 51-75 mm long consumed 100% zooplankton. Kimsey (14) reports that larval Gila in Eagle Lake, California, feed on rotifers, diatoms, desmids, and "other microscopic material" and that 83.3 % of the adult stomach contents contained animal plankton and 46.6 % of the stomachs contained higher plant remains, principally Potamogeton. The food habits of fluvial species of this genus have been investigated. Cross (08) found that filamentous algae comprised 90 pct. of the vol. of the gut contents of Gila robusta seminuda in the Virgin River with invertebrates occupying the other 10%. The adult of Gila robusta in the Colorado River drainage are generally omnivorous (08,09) while the young are primarily insectivorous shifting more to plant material as they mature (09). This same pattern is exhibited by Gila robusta seminuda in the Virgin River (10) and Gila robusta ssp. in the Moapa River (13). Stomach contents of adult Gila cypha primarily contained Chironomids and Simuliids (11) and Gila elegans is also generally considered to be an insectivore (12). HOME RANGE/TERRITORY: No information is available concerning territoriality of this species. In addition, no significant information exists concerning territoriality for any other species of endangered western chub. PERIODICITY: Some periodicity for Gila bicolor obesus in Pyramid Lake has been noted by Snyder (04). Large numbers of this species will invade newly flooded shallows in the evenings and return to deeper water in the morning (04). Seasonal periodicity is also indicated (06) with spawning fishes moving into the more shallow areas of the lake in the Life History - 1 (DRAFT) - Life History Species CHUB, TUI, OWENS Species Id ESIS251018 Date 14 MAR 96 spring and summer and returning to deeper water in the fall and winter. MIGRATION PATTERNS: Fish of this genus are considered non-migratory, however, Snyder (04) states the following concerning Gila bicolor ssp. in Pyramid Lake "Far out and up and down the shores the water fairly boiled. Spring had come, and with it, in the dim light of the early morning, myrids of fishes from the depths of the lake. Daylight revealed them everywhere, along the shore, among the bolders, and in the algae, hovering in enormous schools over the bars and moving about in the clear water of the sheltered bay. Snyder (04) continues "Residents report that during the summer large numbers of minnows frequent inshore waters, but that in September they disappear and are seen no more until the following spring." La Rivers (06), working in Pyramid Lake 50 years after Snyder (04) states that chubs are quite apparent in the shallows and near the surface of deeper water in the summer and seek the deeper water in the winter. COVER/SHELTER REQUIREMENTS: Snyder (04) reports that apparent spawning aggregations of lacustrine Gila bicolor obesus gather about manmade structures, fallen trees, and other sheltered areas in Pyramid Lake and La Rivers (06) reports that schools of Gila bicolor ssp. in Pyramid Lake will seek deeper water when disturbed. Several studies have touched on the cover/shelter requirements of fluvial species of this genus. For example, Miller (17) has noted that Gila nigrescens is most abundant in habitats which include pools to about one meter deep, with riparian and submerged cover, shade, undercut banks, and submerged shrubs. Juveniles of this species are more often found in shallow water without cover. This species is also usually found in streams over a substrate of sand, gravel, and cobble, with some occasional fine mud or silt (17). Adult Gila robusta jordani prefer deep pools with a slow current (13) while Gila cypha is found in canyon habitats associated with deep (15m), fast water over boulder substrates. Cross (08) found Gila robusta seminuda in the Virgin River to be most commonly associated with bottoms of sand and silt (70%) and, less commonly (30%), associated with gravel and rubble. Cross (08) also found G. r. seminuda more often in runs and less commonly (35%) in pools and riffles. In terms of riparian cover, most of the G. r. seminuda captured by Cross (08) were taken from areas of cover including boulders, deadfalls, overhanging trees and/or undercut banks. It is likely, when one considers the past extensive distribution of Gila bicolor snyderi, that this species may have been found in association with a number of different habitat types. REPRODUCTIVE SITE REQUIREMENTS: Kimsey (14) reports that tui chubs in Eagle Lake, California, spawn in about three feet of water in temperatures of about 55 to 60 F in aquatic plant beds of Mirophyllum, Ceratophyllum, and Potamogeton. In laboratory experiments he found that eggs attached to the leaves or stems of these plants hatched while those that fell to the substrate Life History - 2 (DRAFT) - Life History Species CHUB, TUI, OWENS Species Id ESIS251018 Date 14 MAR 96 failed to hatch. REPRODUCTIVE CHARACTERISTICS: Kimsey (14) reports that spawning of Gila bicolor takes place in Eagle Lake, California, during the third spring of life when fish average about six inches SL. Kucera (15) reports peak spawning activity in Pyramid Lake in July. Snyder (04) indicates that pinkish scales and yellow belly tints are prominent during the breeding season. Kucera (14) reports that most male and female tui chub in Pyramid Lake are sexually mature at two years and all by three years. Three-year-old females average 232 mm in fork length and produced an average of 15,135 ova. Four-year-old females averaged 277 mm in fork length and produced an average of 31,622 ova. Ova production across age classes III-VII averaged 23,292 with a range of from 6,110 to 68,933. Newly extruded eggs are from 1.5 to 1.9 mm in diameter. They are pale orange when extruded changing to a light straw-yellow. An 11 inch female produced 11,200 eggs and dissection indicated that eggs do not mature all at the same time. The yolk sac has been absorbed and the larvae are well developed upon hatching. Sex ratios (male:female) of Gila bicolor in Pyramid Lake are reported by Kucera (15) as follows: April May June July August 1:2 1:1.06 1.6:1 1:1.14 1.27:1 The female gonadal somatic index (GSI-percent gonad weight/total body weight) occurred from February through May while the greatest increase in somatic growth (fork length) took place in fall and winter (15). PARENTAL CARE: No information exists on parental care by this species. Such care is unlikely and is not exhibited by other species of Gila. POPULATION BIOLOGY: Very little information exists about the population biology of Gila bicolor snyderi. Limiting factors include the loss and degradation of habitat and predation or competition from introduced exotic fishes. In the lacustrine Pyramid Lake populations of Gila bicolor ssp. larval abundance was greatest in mid-July (07). The transition from meta-larva to juveniles occurred at about 15 mm TL (07). Young fish showed almost continous growth in length and weight for the first two years (16). Following that time peak growth occurred in fall and winter with low growth occurring in spring and early summer (16). Growth rates peak when periphyton and macro-invertebrates peak. Cooper (19) found that major growth occurs during the first year in Walker Lake. Fish 2-3 months old ranged in length from 72-115 mm and averaged 91 mm. The opportunities to study Gila bicolor snyderi under natural conditions have, essentially, been eliminated because of loss of natural habitat. Past collections demonstrate that this fish once Life History - 3 (DRAFT) - Life History Species CHUB, TUI, OWENS Species Id ESIS251018 Date 14 MAR 96 occupied a wide variety of habitats ranging from small springs to the Owens River. A few still remain in shallow stream-side areas of the Owens River (18). The other two remaining viable populations consist of a few hundred individuals in two headwater springs of Hot Creek. SPECIES INTERRELATIONSHIPS: Illegal introductions of largemouth bass (Micropterus salmoides) into the Owens Valley Native Fish Sanctuary and the presence of brown trout (Salmo trutta) in the Owens River below Crowley Lake have had major negative impacts on Gila bicolor snyderi (05,15). This fish sanctuary is managed by the California Dept. of Fish and Game (20). La Rivers (06) reports that Lahontan cutthroat trout (Salmo clarki henshawi) prey on Gila bicolor ssp. in Pyramid Lake and Kennedy (16) states that Gila bicolor ssp. is the major food source of this trout. OTHER LIFE HISTORY DESCRIPTOR: No additional information is available for this species. Life History - 4
                           (DRAFT) - Management Practices
                              Species CHUB, TUI, OWENS
                                Species Id ESIS251018
                                   Date 14 MAR 96



MANAGEMENT PRACTICES

RESULT MANAGEMENT PRACTICE Beneficial Maintaining/Controlling Water Flow Beneficial Controlling water levels Beneficial Transplanting wild animals Adverse Hybridization Existing Hybridization Adverse Predation Existing Predation Adverse Flooding Existing Flooding Adverse Irrigating Existing Irrigating Adverse Reservoirs Existing Reservoirs Adverse Migration barriers Existing Migration barriers Adverse Exotic/Feral/Introducted Species Existing Exotic/Feral/Introducted Species COMMENTS ON MANAGEMENT PRACTICES - Ichthyological surveys during the 1930's and 1940's revealed that Owens Tui chubs were common in various aquatic habitats in the Owens basin. However, over the past 3-4 decades these habitats have been modified, streams have been diverted, and rivers have been dammed. Modifications include a system of diversion structures and aqueducts used to conduct the water to the Los Angeles basin. In general these habitat alterations are the result of high water demand, particularly by the city of Los Angeles (05). Introduction of exotic fishes has also been a major factor in the decline of the Owens tui chub. Predation by trout, particularly brown trout, Salmo trutta, has probably reduced Owens tui chub numbers. Hybridization with the Lahontan tui chub, G.b. obesa, has occurred extensively throughout the Owens basin. The Lahontan tui chubs were probably introduced as bait fish by anglers (05). Trout are present in the remaining two locations where Owens tui chubs are still present, but the impact of their predation on the tui chubs is presently unknown. Future threats include: 1) any significant alterations of the habitats where the chubs still occur, 2) introduction of closely related tui chubs (e.g. Lahontan tui chubs) which would hybridize with the Owens tui chubs, and possibly 3) geothermal development. UNAPPROVED PLAN: At present, no recovery plan for the Owens tui chub exists. The technical draft is due to be completed in fiscal year 1988. Management Practices - 1 (DRAFT) - Management Practices Species CHUB, TUI, OWENS Species Id ESIS251018 Date 14 MAR 96 The management actions that are recommended by the field biologist at the Sacramento field office of the U.S. Fish and Wildl. Serv. for the recovery of the Owens tui chub deal with the translocation of individuals from present populations to similar habitats and/or the maintenance of the water table, flow, and stream banks. At this time, no suitable translocation site has been selected to expand populations and occurrence. Sustaining the present populations involves keeping the existing habitats as suitable as possible. Management Practices - 2
                                   (DRAFT) - References
                                 Species CHUB, TUI, OWENS
                                  Species Id ESIS251018
                                      Date 14 MAR 96



     

References

***** REFERENCES FOR ALL NARRATIVES EXCEPT N-OCCURRENCE ***** 01 Bailey, R.M. and T. Uyeno. 1964. Nomenclature of the blue chub and the tui chub, Chprinid Fishes from the Western United States. Copeia (1964):238-239. 02 Gilbert, C.H. 1893. Report on the fishes of the Death Valley Exp. collected in southern California and Nevada in 1891, with descriptions of new species. N. Amer. Fauna 7:229-234. 03 Miller, R.R. 1973. Two new fishes, Gila bicolor snyderi and Catostomus fumeiventris, from the Owens River Basin, California. Occ. Pap. Mus. Zool. Univ. Mich 667:1-19. 04 Snyder, J.O. 1917. An account of some fishes from Owens River, California. Proc. U.S. Nat. Mus. 54:201-205. 05 Williams, J.E. 1985. Endangered and threatened wildlife & plants; Endangered status and critical habitat designation for the Owens Tui Chub. Fed. Reg. 50(150):31592-31597. 06 La Rivers, I. 1962. Fishes and fisheries of Nevada. State Printing. Off., Carson City. 782 pp. 07 Galat, D.L. and N. Vucinich. 1983. Food partitioning between young of the year of two sympatric tui chub morphologies. Trans. Am. Fish. Soc. 112:486-497. 08 Cross, J.N. 1978. Status and ecology of the Virgin River roundtail chub, Gila robusta seminuda (Osteichthy:Cyprinidae). Southwest Nat. 23(3):519-527. 09 Vanicek, C.D. and R.H. Kramer. 1969. Life History of the Colorado squawfish, Ptychochelius lucius, and the Colorado chub, Gila robusta, in the Green River in Dinosaur National Monument, 1964-1966. Trans. Am. Fish. Soc. 98:193-208. 10 Greger, P. 1982. Feeding relationships of the fishes of the Virgin River. Unpubl. M.S. thesis, Univ. Nev., Las Vegas. 38 pp. 11 Keading, L.R. and M.A. Zimmerman. 1983. Life history and ecology of the humpback chub in the Little Colorado River of the Grand Canyon. Trans. Am. Fish. Soc. 112:577-594. 12 Valdez, R.A. and G.H. Clemmer. 1982. Life history and prospects for recovery of the humpback and bonytail chub. pp 109-119. In: Miller et al. (eds), Fishes of the Upper Colorado River System, present and future. West. Div. Am. Fish. Soc., Bethesda, MD. 13 Hardy, T.B. 1982. Ecological interactions of the introduced and native fishes in the outflow of Ash Spring, Lincoln Co., Nevada. Unpubl. M.S. thesis, Univ. Nev., Las Vegas. 79 pp. 14 Kimsey, J.B. 1954. The life history of the tui chub, Siphateles bicolor (Girard), from Eagle Lake, California. Calif. Fish & Game 40:395-410. 15 Kucera, P.A. 1978. Reproductive biology of the tui chub, Gila bicolor, in Pyramid Lake, Nevada. Great Basin Nat. 38(2):203-207. 16 Kennedy, J.L. 1983. Seasonal growth of the tui chub, Gila bicolor, in Pyramid Lake, Nevada. Great Basin Nat. 43(4):713-716. 17 Miller, R.R. and B. Chernoff. 1979. Status of populations of the endangered Chihuahua chub, Gila nigrescens, in New Mexico and Mexico. In: Proc. Desert Fish. Council XI:74-84. 18 Sada, D. 1986. Pers. comm. U.S. Fish and Wildl. Serv., Reno, NV. References - 1 (DRAFT) - References Species CHUB, TUI, OWENS Species Id ESIS251018 Date 14 MAR 96 19 Cooper, J.J. 1985. Age, growth, and food habits of tui chub, Gila bicolor, in Walker Lake, Nevada. Great Basin Nat. 45(4):784-788. 20 Lorentzen, E. 1987. Pers. comm. U.S. Fish and Wildl. Serv., Sacramento, CA. ***** REFERENCES FOR N-OCCURRENCE NARRATIVE ONLY ***** 01 Brown, S.D. 1981. Owens Tui Chub progress report (4/27/81). Unpub. report, CA Dept. Fish & Game, Bishop, CA. 02 Brown, S.D. 1982. Owens Tui Chub progress report (8/30/82). Unpub. report, CA Dept. Fish & Game, Bishop, CA. 03 McEwan, D.R. 1985. Scale analysis of tui chubs from the Hot Creek Headsprings. Unpubl. memo. CA Dept. Fish & Game, Bishop, CA. 04 McEwan, D.R. 1985. Observations of Owens tui chubs, Hot Creek Headsprings. Unpubl. memo. CA Dept. Fish & Game, Bishop, CA. 05 Miller, R.R. 1973. Two new fishes, Gila bicolor snyderi and Catostomus fumeiventris, from the Owens River Basin, CA. Occ. Papers Mus. Zool., Univ. Mich. 667:1-19. 06 Williams, J.E. 1985. Endangered and threatened wildlife and plants; Endangered status and critical habitat designation for the Owens tui chub. Fed. Reg. 50(150):31592-31597. 07 Wong, D.A. 1985. Owens chub electroshocking surveys, July 1985. Unpubl. memo. CA Dept. Fish & Game, Bishop, CA. 08 Lorentzen, E. 1987. Pers. comm. U.S. Fish and Wildl. Serv., Sacramento, CA. References - 2