(DRAFT) - Taxonomy
                        Species SPRINGFISH, WHITE RIVER, HIKO
                                Species Id ESIS251021
                                   Date 14 MAR 96



TAXONOMY

NAME - SPRINGFISH, WHITE RIVER, HIKO OTHER COMMON NAMES - SPRINGFISH, WHITE RIVER, HIKO; KILLIFISH, WHITE RIVER; MINNOW and BAILEY ELEMENT CODE - CATEGORY - Fish PHYLUM AND SUBPHYLUM - CHORDATA, CLASS AND SUBCLASS - OSTEICHTHYES, ORDER AND SUBORDER - ATHERINIFORMES, FAMILY AND SUBFAMILY - GOODEIDAE, GENUS AND SUBGENUS - CRENICHTHYS, SPECIES AND SSP - BAILEYI, GRANDIS SCIENTIFIC NAME - CRENICHTHYS BAILEYI GRANDIS AUTHORITY - TAXONOMY REFERENCES - COMMENTS ON TAXONOMY - Hiko White River Springfish Crenichthys baileyi grandis (Williams and Wilde, 1981) KINGDOM: Animal GROUP: Fish PHYLUM: Chordata CLASS: Osteichthyes ORDER: Atheriniformes FAMILY: Goodeidae Crenichthys baileyi ssp. is a small (2.5 inches maximum length), deep-bodied fish, olivaceous dorso-laterally and silver ventrally, with double rows of spots on the sides which may be connected (14). Breeding males exhibit more intense coloration than females, with mid-dorsal markings becoming very dark (almost black) contrasting with light, sometimes yellow, sides above the fused spots (13). Lack of ventral fins and bicuspid teeth distinguish this genus from other members of the Cyprinodontidae (10). LaRivers (14) summarizes the taxonomic history of C. baileyi which was originally described as Cyprinodon macularius baileyi (08). Following description of the new genus Crenichthys (10), Cyprinodon macularius baileyi was renamed Crenichthys baileyi (16). Further taxonomic refinement was made recently by Williams and Wilde (20) who described 5 new subspecies of C. baileyi (C.b. albivalis, C.b. thermophilus, C.b. baileyi, C.b. moapae, and C.b. Taxonomy - 1 (DRAFT) - Taxonomy Species SPRINGFISH, WHITE RIVER, HIKO Species Id ESIS251021 Date 14 MAR 96 grandis) based on significant morphological differences among populations from isolated springs along the pluvial White River, Nevada. The Hiko White River springfish can be distinguished from other C. baileyi subspecies by its larger size (adults average larger than 40 mm SL) and deeper coloration. C.b. grandis males exhibit much more yellow, which extends from the ventral surface of the head and body posteriorly onto the caudal fin, which becomes deep orange (20). Other, more specific, distinguishing characteristics include a longer head than C.b. albivalis, and more dorsal and anal fin rays than C.b. thermophilus and C.b. baileyi (20). Following Parenti (22), Deacon and Williams (07) recently placed the genera Crenichthys and Empetrichthys in the family Goodeidae. Type materials include 1 male holotype (UMMZ 2033336) and allotype (UMMZ 203337), and 29 paratopotypes (ASU 3942) collected 3-4 June, 1966 by J.E. Deacon from Hiko Spring. There are an additional 44 paratopotypes (UNLV 142) collected 4 June, 1964 by J.E. Deacon, and 40 paratopotypes (UMMZ 124998) collected 28 August, 1938 by C.L. Hubbs. Taxonomy - 2
                                  (DRAFT) - Status
                        Species SPRINGFISH, WHITE RIVER, HIKO
                                Species Id ESIS251021
                                   Date 14 MAR 96



STATUS

Coded Status E: Federal Endangered COMMENTS ON STATUS - U.S. STATUSES AND LAWS: The Hiko White River springfish (Crenichthys baileyi grandis) has been designated as Endangered pursuant to the Endangered Species Act of 1973 (50 CFR 17.11; P.L. 93-205, 87 Stat. 884; 16 U.S.C. 1531-1540), as amended. The subspecies has this status wherever found including the State of Nevada. Critical Habitat has been designated in Lincoln County, Nevada in portions of T4S, R60E, Sec. 14 and T5S, R60E, Sec. 10 and 11 including Hiko and Crystal Springs and associated outflows, plus surrounding land areas for a distance of 50 feet from the springs and outflows (50 CFR 17.95(e)). This subspecies is protected by the Lacey Act (P.L. 97-79, as amended; 16 U.S.C. 3371 et seq.) which makes it unlawful to import, export, transport, sell, receive, acquire, or purchase any wild animal (alive or dead including parts, products, eggs, or offspring): (1) in interstate or foreign commerce if taken, possessed, transported or sold in violation of any State law or regulation, or foreign law; or (2) if taken or possessed in violation of any U.S. law, treaty, or regulation or in violation of Indian tribal law. It is also unlawful to possess any wild animal (alive or dead including parts, products, eggs, and offspring) within the U.S. territorial or special maritime jurisdiction (as defined in 18 U.S.C. 7) that is taken, possessed, transported, or sold in violation of any State law or regulation, foreign law, or Indian tribal law. RESPONSIBLE FEDERAL AGENCIES: USFWS -Responsible for the management/recovery, listing, and law enforcement/protection of this species. BLM -Responsible for the law enforcement/protection of this species with applicable State and Federal laws on public land under their control (43 CFR 4140). Also responsible for management/recovery on Bureau of Land Management lands. All Federal agencies have responsibility to ensure that any action authorized, funded, or carried out by that agency is not likely to jeopardize the continued existence of the species or result in the destruction or adverse modification of Critical Habitat (50 CFR 402), and to utilize their authorities to carry out programs for the conservation of the species. Status - 1 (DRAFT) - Status Species SPRINGFISH, WHITE RIVER, HIKO Species Id ESIS251021 Date 14 MAR 96 STATE STATUSES AND LAWS: STATE: Nevada DESIGNATED STATUS: Endangered ADMINISTRATIVE AGENCY: Nevada Department of Wildlife STATE STATUTE: Protected by Nevada Rev. Stat. 501.818, 501.351, 503.584, 503.585, 503.587, 503.588, and 503.589. INTERNATIONAL STATUSES, TREATIES, AND AGREEMENTS: The species Crenichthys baileyi (White River springfish is listed as vulnerable in the 1986 IUCN Red List of Threatened Animals. ECONOMIC STATUSES: None. 82/12/30:47 FR 58454/58460 - Notice of review 83/06/14:48 FR 27273/27274 - Notice of review 84/05/07:49 FR 19360/19363 - Proposed rule - Endangered w/ Crit. Hab. 85/09/27:50 FR 39123/39128 - Final rule - Endangered w/ Crit. Hab. Status - 2
     

HABITAT ASSOCIATIONS

HABITAT - AQUATIC INLAND AQUATIC LAND USE - Shrub and Brush Rangeland Deciduous Forest Land Streams and Canals Forested Wetland NATIONAL WETLAND INVENTORY CODES NWI NWICLS NWIMOD NWISPEC Riverine, upper perennial SB2 Riverine, upper perennial SB1 Palustrine UB2 Palustrine UB1 Palustrine SS1 Palustrine AB4 Palustrine AB2 Palustrine AB1 COMMENTS ON HABITAT ASSOCIATIONS - The Hiko White River springfish occurs in Hiko and Crystal Springs located in the Pahranagat Valley of southern Nevada, in the northern Mohave Desert. These two springs are typical of thermal desert springs of southern Nevada, as described by Bradley and Deacon (01). These habitats include relatively deep (1-2 meters) source pools and associated effluent streams. Aquatic vegetation becomes abundant during the spring and summer, and may provide valuable cover for springfish (12). Common aquatic vegetation includes filamentous algae, and the emergent macrophytes Rorippa nasturtium-aquaticum, Potamogeton spp., Najas marina, and Chara zeylanica. Shoreline vegetation includes members of the Chenopodicaceae, the spike-rush (Eleocharis parishi), and cattails (Typha augustifolia). Tree species present around portions of the shoreline of these springs include ash (Fraxinus velutina), cottonwood (Populus fremontii), and willows (Salix sp.) (12). Substrates are typically unconsolidated sand and silt with some areas of gravel. Spawning in C.b. moapae was described by Kopac (13) and may be similar in C.b. grandis. He observed spawning to take place in areas of dense vegetation, with the vegetation acting as a substrate for attachment of fertilized eggs. Specific environmental parameters required by the Hiko White River springfish have not been evaluated. Early investigations dealt mainly with the ability of C. baileyi ssp. to withstand high temperatures and low dissolved oxygen concentrations (11,16). Environmental parameters associated with Hiko and Crystal springs have been variously reported and include temperatures ranging from 19.5 degrees to 26.5 degrees Celsius (Hiko) and 24.5 degrees to 28.0 degrees Celsius (Crystal) (06,11,20); pH of 7.3 (11); and dissolved oxygen concentrations ranging from 2.9 ppm to 9.0 ppm (Hiko) and 1.6 ppm to 8.8 ppm (Crystal) (06,11,20). Habitat Associations - 1
                                (DRAFT) - Food Habits
                        Species SPRINGFISH, WHITE RIVER, HIKO
                                Species Id ESIS251021
                                   Date 14 MAR 96



FOOD HABITS

TROPHIC LEVEL - OMNIVORE LIFESTAGE FOOD FOOD PART General Detritus General Diatoms General Crustaceans General Molluscs General Algae General Arthropods Food Habits - 1
                         (DRAFT) - Environment Associations
                        Species SPRINGFISH, WHITE RIVER, HIKO
                                Species Id ESIS251021
                                   Date 14 MAR 96



ENVIRONMENTAL ASSOCIATIONS

G = General A = Adult LIM = Limiting RA = Resting Adult J = Juvenile FA = Feeding Adult RJ = Resting Juvenile BA = Breeding Adult FJ = Feeding Juvenile P = Pupae L = Larvae E = Egg RL = Resting Larvae FL = Feeding Larvae
LIFESTAGE ENVIRONMENTAL ASSOCIATIONS G G Aquatic Features: Pool areas G Aquatic Features: Springs [flowing] Environment Associations - 1
                               (DRAFT) - Life History
                        Species SPRINGFISH, WHITE RIVER, HIKO
                                Species Id ESIS251021
                                   Date 14 MAR 96



LIFE HISTORY

FOOD HABITS: Specific food habits of the Hiko White River springfish have not been described, but food habits of closely related subspecies of C. baileyi have and may be quite similar. Both C.b. albivalis and C.b. moapae have been shown to be omnivorous (15,18), which agrees with Constantz (02) who suggests that simple, short food chains of desert springs do not allow for diet specialization. C.b. albivalis was described as an opportunistic feeder consuming 52.2 percent plant material, 32.2 percent detritus, and 15.3 percent animal matter (18). Of the plant material consumed, filamentous algae was greatest in both volume and frequency of occurrence, followed by vascular plant material and diatoms. Chironomidae larvae and trichoptera comprised the largest component of the animal matter consumed. The diet of C.b. moapae was comprised of 49.8 percent detritus, 37.8 percent plant material, and 12.4 percent animal matter. Filamentous algae was by far the most abundant and frequent plant material ingested (95.1 percent), while gastropoda, amphipoda, trichoptera, and lepidoptera were the most common animals ingested (15). Mean digestive tract length to standard length ratio for C.b. albivalis was reported to be 1.43, and the digestive tract length to total length for C.b. moapae was 1.21, further substantiating an omnivorous lifestyle (15,18). Seasonal variation in food availability and diet of the Hiko White River springfish has not been described. Neither have variations in diet of different life stages. Assuming that what is known about the food habits of other C. baileyi subspecies is similar for C.b. grandis, availability of filamentous algae and aquatic macroinvertebrates would be important for the survival of this fish. HOME RANGE/TERRITORY: Details of the home range and territorial behavior in the Hiko White River springfish have not been assessed. However, Constantz (02) suggests that large, dominant, brightly colored males of spring inhabiting fishes aggressively defend territories in order to monopolize reproduction. PERIODICITY: Deacon and Wilson (06) describe a bimodal daily activity cycle for C.b. grandis in Crystal Spring. Peaks in activity were apparently associated with sunrise and sunset. Since the time of this study however, exotic fish have been introduced into Hiko and Crystal springs which may have caused a reduction in activity levels, as was hypothesized for C.b. baileyi in Ash Spring (06). No information is available on seasonal or yearly activity cycles of this fish. MIGRATION PATTERNS: The Hiko White River springfish is non-migratory. COVER/SHELTER REQUIREMENTS: Life History - 1 (DRAFT) - Life History Species SPRINGFISH, WHITE RIVER, HIKO Species Id ESIS251021 Date 14 MAR 96 Specific cover requirements of the Hiko White River springfish have been characterized. Since it occurs in a spring pool habitat however, it is likely that floating algal mats and emergent aquatic vegetation provide the most important cover. REPRODUCTIVE SITE REQUIREMENTS: Reproduction site requirements of this subspecies have not been assessed or described. Spawning in C.b. moapae was described by Kopac (13) and may be similar in C.b. grandis. He observed spawning to take place in areas of dense vegetation, with the vegetation acting as a substrate for attachment of fertilized eggs. REPRODUCTIVE CHARACTERISTICS: The reproduction biology and life history of the Hiko White River springfish is unknown. However, Constantz (02) does describe general life history traits for fish species inhabitting thermal desert springs which include a year-round breeding season, short time to first breeding, low fecundity, and large investment per offspring. In addition, Kopec (13) describes details of the breeding behavior of C.b. moapae which may be similar to that of C.b. grandis. These details include: 1) reproductive males are more brightly colored than females, 2) courting behavior and spawning occur in and around dense vegetation, 3) the male and female go into an S-shaped clasp and vibrate very fast while lying on their sides. During this phase the males' anal fin is folded under the females genital opening to enhance sperm transfer, 4) a single egg is layed and fertilized at a time, 5) 10 to 17 eggs represent one spawning, and 6) incubation period is 5 to 7 days. PARENTAL CARE: This species is not known to provide parental care to its offspring. POPULATION BIOLOGY: Details of the population biology of this species are unknown at this time. One factor which has been reported to limit population growth and cause increased mortality in this species is interaction with exotic fishes introduced into its habitat. SPECIES INTERRELATIONSHIPS: The most significant species interrelationships affecting the Hiko White River springfish occur between it and exotic fishes introduced into its habitat. As is the case in most isolated desert springs, there is a strong negative relationship between the abundance of exotic fish species and the abundance of endemics (05,19). Exotic species introduced into Hiko and Crystal springs include the convict cichlid (Cichlosoma nigrofasciatum), sailfin molly (Poecilia latipinna), shortfin molly (P. mexicana), mosquitofish (Gambusia affinis) and largemouth bass (Micropterus salmoides) (Hiko only) (03, 09,20). These fishes reduce populations of springfish through competition, predation, and possibly the introduction of parasites (04,05). Life History - 2 (DRAFT) - Life History Species SPRINGFISH, WHITE RIVER, HIKO Species Id ESIS251021 Date 14 MAR 96 OTHER LIFE HISTORY DESCRIPTORS: None. Life History - 3
                           (DRAFT) - Management Practices
                        Species SPRINGFISH, WHITE RIVER, HIKO
                                Species Id ESIS251021
                                   Date 14 MAR 96



MANAGEMENT PRACTICES

RESULT MANAGEMENT PRACTICE Beneficial Restricting/regulating human disturbance of populations Beneficial Maintaining undisturbed/undeveloped areas Beneficial Land Acquisition Beneficial Controlling/Restricting Agricultural Practice Beneficial Reforestation Beneficial Transplanting wild animals Beneficial Disease Control Measures Beneficial Controlling/Removing Exotic Vertebrates Beneficial Controlling/Removing Feral Animals Adverse Low Gene Pool Existing Low Gene Pool Adverse Parasites Existing Parasites Adverse Predation Existing Predation Adverse Irrigating Existing Irrigating Adverse Dredging Existing Dredging Adverse Competition Existing Competition Adverse Exotic/Feral/Introducted Species Existing Exotic/Feral/Introducted Species COMMENTS ON MANAGEMENT PRACTICES - The Hiko White River springfish is presently listed as an endangered species due to its extremely restricted range, small population size, and because it is threatened by habitat alteration and association with exotic species (17). Physical habitat alteration which has been detrimental to springfish populations includes channelization of spring outflows and impoundment and diversion of water for irrigation (09,19). Exotic fish species which have been introduced into Hiko and Crystal Springs include the convict cichlid, sailfin molly, shortfin molly, carp, mosquitofish, and largemouth bass (07,09,19,20). These exotics have reduced springfish populations through competition, predation, and the possible co-introduction of parasites (04). At the time of its listing, the Hiko White River springfish existed as a single, small population restricted to Crystal Springs (07,09,20). Predation by largemouth bass had caused its extirpation from Hiko Spring in the 1960's. In February, 1985 springfish (Crystal Springs progeny) were re-introduced into Hiko Spring and are now apparently well established, while their numbers in Crystal Springs are quite low (21). In addition, springfish (also Crystal Springs progeny) were introduced into Blue Link Spring, Mineral Co., Nevada by personnel from the University of Nevada, Las Vegas to establish a refuge population, which is also well established (21). Further alteration of existing spring habitats, or introductions Management Practices - 1 (DRAFT) - Management Practices Species SPRINGFISH, WHITE RIVER, HIKO Species Id ESIS251021 Date 14 MAR 96 of exotic fishes will threaten the continued existence of the Hiko White River springfish. UNAPPROVED PLAN: No initiation date for a recovery plan for the Hiko White River springfish is established. The management actions which were recommended for the recovery of the Hiko White River springfish were given by the field biologist in Sacramento. The following items are recommendations for recovery: 1) controlling and removing feral and exotic animals, 2) using disease control measures, 3) transplanting wild individuals, 4) habitat reconstruction, 5) land easements, 6) controlling and restricting development and construction, 7) limiting human access, 8) controlling and restricting agricultural practices. In February, 1985, springfish (Crystal Springs progeny) were re-introduced into Hiko Spring and are now apparently well established. In addition, springfish (also Crystal Springs progeny) were introduced into Blue Link Spring, Mineral County, Nevada by personnel for the University of Nevada, Las Vegas to establish a refuge population, which is also well established. Management Practices - 2
                                   (DRAFT) - References
                          Species SPRINGFISH, WHITE RIVER, HIKO
                                  Species Id ESIS251021
                                      Date 14 MAR 96



     

References

***** REFERENCES FOR ALL NARRATIVES EXCEPT N-OCCURRENCE ***** 01 Bradley, W.G. and J.E. Deacon. 1967. The biotic communitites of southern Nevada. NV St. Mus. Anthr. Papers 13(4):201-295. 02 Constantz, G.D. 1981. Life history patterns of desert fishes. Fishes of N. Am. Deserts. John Wiley and Sons, NY. 137-290 pp. 03 Courtney, W.R. Jr., et al. 1985. Comparative status of fishes along the course of the pluvial White River, Nevada. Southwestern Nat. 30(4):503-524. 04 Deacon, J.E. 1979. Endangered and threatened fishes of the west. Great Basin Nat. Mem. 3:41-64. 05 Deacon, J.E., C. Hubbs, and B.J. Zahuranec. 1964. Some effects of introduced fishes on the native fish fauna of southern Nevada. Copeia 1964:384-388. 06 Deacon, J.E. and B.L. Wilson. 1967. Daily activity cycles of Crenichthys baileyi, a fish endemic to Nevada. Southwestern Nat. 12(1):31-44. 07 Deacon, J.E. and J.E. Williams. 1984. Annotated list of the fishes of Nevada. Proc. Biol. Soc. Wash. 97:103-118. 08 Gilbert, C.H. 1893. Report on the fishes of the Death Valley expedition collected in southern California and Nevada in 1891, with descriptions of new species. N. Amer. Fauna 7:229-234. 09 Hardy, T. 1980. Interbasin area report-1979. Proc. of the Desert Fishes Council. Vol. XI. 16 pp. 10 Hubbs, C.L. 1932. Studies of the fishes of the order Cyprinodontes XII a new genus related to Empetrichthys. Univ. of Mich. Mus. of Zool. Occasional Paper no. 252:1-5. 11 Hubbs, C. and W.F. Hettler. 1964. Observations on the toleration of high temperature and low dissolved oxygen in natural waters by Crenichthys baileyi. Southwestern Nat. 9:245-248. 12 Kanim, N.R. 1986. Pers. comm. Univ. of Nevada, Las Vegas. 13 Kopec, J.A. 1949. Ecology, breeding habits and young stages of Crenichthys baileyi, a cyprinodont fish of Nevada. Copeia 1949:56-61. 14 LaRivers, I. 1962. Fish and fisheries of Nevada. NV St. Fish and Game Commission. Carson City, NV. 512-516 pp. 15 Scoppettone, G.G., et al. 1984. Unpub. data. U.S. Fish and Wildlife Service, Reno, NV. 16 Sumner, F.B. and M.C. Sargent. 1940. Some observations on the physiology of warm spring fishes. Ecology 21:45-54. 17 U.S. Fish and Wildlife Servie. Endangered and threatened wildlife and plants; determination of endangered status for the Hiko White River Springfish. Fed. Reg. 50:37194-37197. 18 Williams, C.D. and J.E. Williams. 1982. Summer food habits of fishes from two springs in east-central Nevada. Southwestern Nat. 27(4):437-445. 19 Williams, J.E., et al. 1985. Endangered aquatic ecosystems in North American deserts with a list of vanishing fishes of the region. J. of the AZ-NV Acad. of Sci. 20(1):1-61. 20 Williams, J.E. and G.R. Wilde. 1981. Taxonomic status and morphology of isolated populations of the White River springfish, References - 1 (DRAFT) - References Species SPRINGFISH, WHITE RIVER, HIKO Species Id ESIS251021 Date 14 MAR 96 Crenichthys baileyi (Cyprinodontidae). Southwestern Nat. 25(4):485-503. 21 Withers, D. 1986. Pers. comm. Nevada Dept. of Wildlife. 22 Parenti, L.R. 1981. A phylogenetic and biogeographic analysis of Cyprinodontiform fishes (Teleostei, Alherinomorpha). Bull. of the Amer. Mus. Nat. Hist. 168:335-557. ***** REFERENCES FOR N-OCCURRENCE NARRATIVE ONLY ***** 01 Courtney, W.R., J.E. Deacon, et al. 1985. Comparative status of fishes along the course of the pluvial White River, Nevada. Southwestern Nat. 30(4):503-524. 02 Deacon, J.E. 1979. Endangered and threatened fishes of the west. Great Basin Nat. Mem. 3:41-64. 03 Deacon, J.E. and J.E. Williams. 1984. Annotated list of the fishes of Nevada. Proc. Biol. Soc. Wash. 97:103-118. 04 Hubbs, C.L. et al. 1974. Hydrographic history of relict fishes of the north-central great basin. Mem. of the CA Acad. of Sci. Vol. VII. 259 pp. 05 LaRivers, I. 1962. Fish and fisheries of Nevada. NV State Fish and Game Comm., Carson City. 512-516 pp. 06 Williams, J.E., D.B. Bowman, et al. 1985. Endangered aquatic ecosystems in North American deserts with a list of vanishing fishes of the region. J. of the AZ-NV Acad. of Sci. 20(1):1-61. 07 Williams, J.E. and G.R. Wilde. 1981. Taxonomic status and morphology of isolated populations of the White River springfish, Crenichthys baileyi (cyprinodontidae). Southwestern Nat. 25(4):485-503. 08 Parker, M.S. 1986. Pers. knowledge. Davis, CA. References - 2