(DRAFT) - Taxonomy
                                  Species WOUNDFIN
                                Species Id ESIS252001
                                   Date 14 MAR 96



TAXONOMY

NAME - WOUNDFIN OTHER COMMON NAMES - WOUNDFIN; FIN and WOUND ELEMENT CODE - CATEGORY - Fish PHYLUM AND SUBPHYLUM - CHORDATA, CLASS AND SUBCLASS - OSTEICHTHYES, ORDER AND SUBORDER - CYPRINIFORMES, FAMILY AND SUBFAMILY - CYPRINIDAE, GENUS AND SUBGENUS - PLAGOPTERUS, SPECIES AND SSP - ARGENTISSIMUS, SCIENTIFIC NAME - PLAGOPTERUS ARGENTISSIMUS AUTHORITY - TAXONOMY REFERENCES - COMMENTS ON TAXONOMY - Woundfin Plagopterus argentissimus Cope, 1874 KINGDOM: Animal GROUP: Fish PHYLUM: Chordata CLASS: Osteichthyes ORDER: Cypriniformes FAMILY: Cyprinidae The woundfin is the most silvery of all American minnows, reflecting blue in bright sunlight (01). The general shape is fusiform with the exception of the flattened head and belly. The fins are large and faleate, the eyes are somewhat reduced, the mouth is nearly horzontal, the lips bear papillae and two barbels, and dermal sense organs are present. The body is tannish dorsally, the sides and belly are silvery. The two ossified, anterior dorsal rays extend into the tip of the fin; hence the common name: woundfin (12) or wound fin (27). This fish rarely exceeds 85 mm, although occasional specimens, usually females, exceed 95 mm (02). "The woundfin is considered the most highly specialized species in the Cyprinid tribe Plagopterini, subfamily Leuciscinae (01). This unique tribe is composed of three genera, two of which, Meda and Plagopterus, are monotypic, and the third, Lepidomeda, is composed of four species. The present taxonomic ranking of the group was Taxonomy - 1 (DRAFT) - Taxonomy Species WOUNDFIN Species Id ESIS252001 Date 14 MAR 96 initiated by Hubbs (03), and is generally accepted. The uniqueness of this compact group of fishes has always impressed ichthyologists. Cope (04) erected a full subfamily, the Plagopterinae, for the genera, and this was widely followed (05,06). Jordan, et al. (07) even erected a separate family, the Medidae, for the group, an action followed only by Tanner (08). The entire taxa is endemic to the lower basin of the Colorado River and its ancestral tributary, the White River" (02). La Rivers, 1962 (27), addressed the fact that Jordon, 1885 and 1889, referred to the species as Meda argentissima. Miller and Hubbs (01) explain how the stated type locality "San Luis Valley, western Colorado (Cope 1874)," was an obvious error. This was one of many such mistakes made by collectors associated with the Wheeler survey of 1871-74. A reported locality of "Colorado Chiquito River, Arizona" (09) was also rejected since there are no other indications that woundfin were ever there, and it was felt that the type series Plagopterus argentissimus was taken from the mainstream of the Virgin River (01). Taxonomy - 2
                                  (DRAFT) - Status
                                  Species WOUNDFIN
                                Species Id ESIS252001
                                   Date 14 MAR 96



STATUS

Coded Status E: Federal Endangered COMMENTS ON STATUS - U.S. STATUSES AND LAWS: The woundfin (Plagopterus argentissimus) has been designated an Endangered species pursuant to the Endangered Species Act of 1973 (50 CFR 17.11; P.L. 93-205, 87 Stat. 884; 16 U.S.C. 1531-1540), as amended. The species has this status wherever found including the States of Arizona, Nevada, and New Mexico except for the Gila River drainage where special rules apply to an experimental population. Special rules specifically address the "nonessential experimental populations" which are being reintroduced into the Gila River drainage (i.e., the Verde River, Gila and Yavapai counties, AZ; Tonto Creek, Gila County, AZ; the Gila River, Graham and Greenlee counties, AZ; the Hassayampa River, Yavapai County, AZ; and the San Francisco River, Greenlee County, AZ) begining 1986 for a period of ten years (50 CFR, Sec. 17.84b). The experimental populations of woundfin are treated as if they were Threatened. Non-essential experimental populations not occurring on National Park or National Wildlife Refuge lands are treated as "proposed" species for purposes of Section 7, interagency cooperation (50 CFR 17.84(b)). This species is protected by the Lacey Act (P.L. 97-79, as amended; 16 U.S.C. 3371 et seq.) which makes it unlawful to import, export, transport, sell, receive, acquire, or purchase any wild animal (alive or dead including parts, products, eggs, or offspring): (1) in interstate or foreign commerce if taken, possessed, transported or sold in violation of any State law or regulation; or (2) if taken or possessed in violation of any U.S. law, treaty, or regulation or in violation of Indian tribal law. It is also unlawful to possess any wild animal (alive or dead including parts, products, eggs, and offspring) within the U.S. territorial or special maritime jurisdiction (as defined in 18 U.S.C. 7) that is taken, possessed, transported, or sold in violation of any State law or regulation, foreign law, or Indian tribal law. Status - 1 (DRAFT) - Status Species WOUNDFIN Species Id ESIS252001 Date 14 MAR 96 RESPONSIBLE FEDERAL AGENCIES: USFWS -Responsible for the management/recovery, listing, and law enforcement/protection of this species. BIA -Responsible for the law enforcement/protection of this species with applicable State and Federal laws on public lands under their control. Also responsible for management/recovery on Bureau of Indian Affairs lands. BLM -Responsible for the law enforcement/protection of this species with applicable State and Federal laws on public land under their control (43 CFR 4140). Also responsible for management/recovery on Bureau of Land Management lands. NPS -Responsible for the law enforcement/protection of this species with applicable State and Federal laws on public lands under their control. Also responsible for conservation (Nat. Park System Organic Act - 16 U.S.C. 1, 2-3)/management/recovery on National Park Service lands. Taking, possessing, or disturbing of Federally listed species is prohibited on NPS lands (36 CFR 2.1, 2.2, and 2.3). USFS -Responsible for the law enforcement/protection of this species with applicable State and Federal laws on public lands under their control. Also responsible for management/recovery on Forest Service lands. The Forest Service is responsible for integrating management, protection, and conservation of Federally listed species into the Forest Planning process (36 CFR 219.19 and 219.20). Management practices that would cause detrimental changes in water temperature or composition, water course blockage, or sediment deposits within 100 feet of the edges of perennial streams, lakes or other bodies of water are prohibited (36 CFR 219.27(e)). All Federal agencies have responsibility to ensure that any action authorized, funded, or carried out by that agency is not likely to jeopardize the continued existence of the species or result in the destruction or adverse modification of Critical Habitat (50 CFR 402), and to utilize their authorities to carry out programs for the conservation of the species. STATE STATUSES AND LAWS: STATE: Arizona, California DESIGNATED STATUS: Recognized Endangered ADMINISTRATIVE AGENCY: Arizona Game and Fish Department California Department of Fish and Game STATUTE: Arizona Revised Statutes 17-201, 211, 214 and 231, Arizona Game and Fish Commission, Sec 17-23(B)7 California Fish and Game Code, Sec 2000, 2002 and 2013 Status - 2 (DRAFT) - Status Species WOUNDFIN Species Id ESIS252001 Date 14 MAR 96 STATE: Nevada DESIGNATED STATUS: Rare ADMINISTRATIVE AGENCY: Nevada Department of Fish and Game STATUTE: Nevada Administrative Code 503.584, 503.589 Nevada Revised Statutes 501.105, 501.181, 501.187 STATE: Utah DESIGNATED STATUS: Recognized Endangered ADMINISTRATIVE AGENCY: Utah Department of Natural Resources STATUTE: Utah Code Annotated 23-13-2, 23-20-1, 23-13-5, 23-13-13 and 23-13-14 INTERNATIONAL STATUSES, TREATIES, AND AGREEMENTS: The woundfin is listed in CITES-II, the IUCN Red Data Book for Pices (1977), and by the Convention on Nature Protection and Wildlife Preservation in the Western Hemisphere, 1970. ECONOMIC STATUSES: The woundfin has no commercial value. It has cultural value in that its Endangered status may have an impact on human population growth in the Virgin Valley; growth that if not planned would effectively dry the Virgin River and extirpate the woundfin. 70/10/13:35 FR 16047/16048 - Listed as Endangered 77/02/22:42 FR 10462/10488 - CITES II listing 77/11/02:42 FR 57329/57330 - Proposed designation of Critical Habitat 79/03/06:44 FR 12382/12384 - Removal of proposed Critical Habitat 79/05/21:44 FR 29566/29567 - Five year review 84/04/10:49 FR 14149/14152 - Determ of status of introduced population 85/07/22:50 FR 29901/29909 - Five year review 85/07/24:50 FR 30188/30195 - Reintroduced experimental pop. status 85/09/30:50 FR 39691/ - Correction, final rule Status - 3
     

HABITAT ASSOCIATIONS

HABITAT - AQUATIC INLAND AQUATIC LAND USE - Cropland and Pasture Orchards, Groves, Vineyards, Nurseries, Shrub and Brush Rangeland Streams and Canals Reservoirs NATIONAL WETLAND INVENTORY CODES NWI NWICLS NWIMOD NWISPEC Riverine, upper perennial UB2 COMMENTS ON HABITAT ASSOCIATIONS - Woundfin have most often been collected from runs over sand bottoms, near some cover. Water depths average .34 cm (+ or - 19) and currents average 0.48 m/sec (+ or - 0.23) (12). They have shown a preference for physically unmodified habitats, but are also found in areas that have been greatly modified. Woundfin reach peak numbers in areas with mobile sand bottoms where the substrate shifts slowly downstream under the influence of near-laminar currents. Depths preferred by woundfin appear to range from 5 to 35 cm but disturbed fish swim quickly into deeper water. Woundfin have been collected from waters with temperatures from 10 to 35.5 degrees C (25). Preferred temperature is not known, but as water temperatures approach 30 degrees C, woundfin leave the shallow water areas and congregate in deeper portions of the stream (13). Larval and post-larval woundfin appear to live inshore after hatching, staying in back waters and along the shoreline in a few millimeters of water, then moving into deeper water as they grow larger (02). Adult fish have been found in flows from 0.095 m/sec to 1.132 m/sec (25). Recently, woundfin have been found in habitats different from those described above, principally in less sandy and faster moving portions of the Virgin River. This is primarily because these areas are less accessible for collecting and had not been sampled during previous work (10). The species has been found to be common or abundant in areas which exhibit pool habitat. Streamflows in the Virgin River below 100 cf/s during the spring are associated with poor reproduction and/or recruitment into the population (14). Mean monthly flows between 30 and 200 cf/s are optimal for spawning, fry development, and recruitment of woundfin. Irrigation diversions have a significant adverse effect on recruitment, probably by reducing flows during spawning and fry development (12). The alteration of agricultural areas, reservoirs, and desert shrub/pinyon-juniper rangeland may adversely effect associated woundfin habitat. Habitat Associations - 1
                                (DRAFT) - Food Habits
                                  Species WOUNDFIN
                                Species Id ESIS252001
                                   Date 14 MAR 96



FOOD HABITS

TROPHIC LEVEL - OMNIVORE LIFESTAGE FOOD FOOD PART General Detritus General Algae General Arthropods Food Habits - 1
                         (DRAFT) - Environment Associations
                                  Species WOUNDFIN
                                Species Id ESIS252001
                                   Date 14 MAR 96



ENVIRONMENTAL ASSOCIATIONS

G = General A = Adult LIM = Limiting RA = Resting Adult J = Juvenile FA = Feeding Adult RJ = Resting Juvenile BA = Breeding Adult FJ = Feeding Juvenile P = Pupae L = Larvae E = Egg RL = Resting Larvae FL = Feeding Larvae
LIFESTAGE ENVIRONMENTAL ASSOCIATIONS G Aquatic Features: Pool areas Environment Associations - 1
                               (DRAFT) - Life History
                                  Species WOUNDFIN
                                Species Id ESIS252001
                                   Date 14 MAR 96



LIFE HISTORY

FOOD HABITS: Woundfin have a poorly delineated stomach, occupying about 20% of the total gut length. Fourteen specimens collected in Aug. 1974 had the following food items: "amorphous material (presumably organic) and sand grains were most abundant; insects were commonly found (aquatic insect larvae, especially diptera, were predominant; ants and other terrestrial insects were rare); filamentous algae and organic detritus occurred in about 1/2 of the individuals; amphipod larvae were found in 2 specimens" (02). Lockhart (16) studied 188 woundfin from all seasons of the year and found them quite unselective in their food habits; filamentous algae (Zygnemataceae) were the most common item found. Insects, mostly diptera and stonefly larvae, were most common in 16 specimens collected in 2 different seasons by Winget and Baumann (17). The intestine length/standard length ratio for woundfin is slightly less than unity, placing it near the division between Odum's (19) carnivorous (<1) and omnivorous (1-3) species. It would seem that algae and organic detritus would not be of much value because of the relative length of the gut. Possibly bacteria, protozoans and micro-algae attached to large algae and detrital particles supply the nutrients or aid in the breakdown of plant material (02). More recent studies have also shown that, although omnivorous, woundfin are at times selective (or choice is limited) and that foods taken vary with location on the river. For example, in Feb at Mesquite, Nevada, woundfin were preying heavily on ceratopogonid larvae. Foods taken at Beaver Dam Wash, Arizona, are more varied than at Mesquite (20). When studied in laboratory aquaria (39 & 208 liters), woundfin at first showed evidence of visual selection of food. Soon after the addition of dried foods woundfin began milling excitedly about the bottom. For the first few weeks they fed on or just above the bottom; but, within a month, most were rising to the surface to feed (12). HOME RANGE/TERRITORY: Movements of woundfin are poorly understood. They are not territorial. Collections over a number of years indicate that they may be very mobile (21). Systematic collections on the Virgin River during recent years show an unexplained disappearance of larger individuals in late summer, indicating either movement or mortality. MIGRATION PATTERNS: This species is non-migratory. COVER/SHELTER REQUIREMENTS: Woundfin prefer shallow moving water (5-35 cm), but move into deeper waters to escape danger (12). As water temperatures approach 30 degrees C, woundfin leave the shallow water areas and congregate in deeper portions of the stream (13). Larval and post-larval woundfin appear to live inshore after hatching, staying in back waters and along the shoreline in a few millimeters of water. Later they begin moving into deeper water as they grow larger (02). REPRODUCTIVE SITE REQUIREMENTS: Life History - 1 (DRAFT) - Life History Species WOUNDFIN Species Id ESIS252001 Date 14 MAR 96 Little is known about spawning site requirements. Deacon (22) observed apparent spawning activity on 17 April 1977 in the Virgin River south of Mesquite, Nevada. Water temperature was 14.5 degrees C. Spawning apparently occurred in deeper pockets in the stream bed in flowing current. More recently, Gregor and Deacon (23) found that woundfin select a substrate of rocks 5-10 cm in diameter in current at a temperature of 25 degrees C. Streamflows in the Virgin River below 100 cf/s during the spring are associated with poor reproduction and/or recruitment into the population (14). Deacon (26) reports that mean flows of 90 to 94 cf/s in the Virgin River in April, May and June coincided with poor reproduction while mean flows of 450 cf/s over the same monthes coincided with good reproductive success. Mean monthly flows between 30 and 200 cf/s are optimal for spawning, fry development, and recruitment of woundfin. Irrigation diversions have a significant adverse effect on recruitment, probably simply by reducing flows during spawning and fry development (12). REPRODUCTIVE CHARACTERISTICS: Information available is limited. The beginning of the reproductive cycle may be related to rising water temperatures, lengthening daylight, and declining spring run-off. It would seem to be to the advantage of the species to spawn after the peak run-off as eggs spawned before run-off would be buried by silt or carried away by the current. Some spawning in sheltered areas during high spring flows may occur (02). Gonads mature in March, April, and May (24). Fry appear in June (12); therefore, we assume that spawning begins in late May. Since fry have been found as late as Aug, limited spawning may occur throughout the summer, but peak activity is probably late May to early June. Deacon (22) found indications that spawning begins about a month earlier in the lower Virgin River than at Laverkin Creek. He observed a female, accompanied by 3-4 smaller males, moving out of a pool into flowing current. More males joined the group as they swam around deeper pockets in the stream bed. After much accelerated activity within the group, the female swam back upstream into the pool. This procedure was repeated several times. In 1980, young were successfully produced in the Dexter Hatchery for the first time, but in small numbers. In 1983 they were produced in sufficient numbers to seriously consider transplants of fry in the near future (10). In 1986, reintroduction efforts commenced in AZ. PARENTAL CARE: Development of young: Fry are first found in shallow areas lateral to the main current and only in the main channel when water levels are low. They are never found in pools containing potential predators such as Gambusia affinis, Lepomis machochirus, Micropterus salmoides, and Gila robusta seminuda. Young-of-the-year woundfin of 20-30 cm have been seen in Aug, and growth appears to be good through Oct. (15). Late fall and winter are the time of highest mortality, coinciding with the period of lowest flows and temperatures, and this mortality appears to be the result of these 2 factors (02). Flows between 30 and 200 cf/s are recommended for the best fry development Life History - 2 (DRAFT) - Life History Species WOUNDFIN Species Id ESIS252001 Date 14 MAR 96 and population recruitment (12). POPULATION BIOLOGY: Lockhart (25) reports that adult mortality appears to be greatest in the period of October through December. The youngest age group begins to appear in June and recruitment of these fish into the main river population begins in June and ends in August. Newly recruited fish show their period of greatest growth from August to October (25). Current limiting factors for the woundfin are the modification and loss of its habitat. Construction of dams and associated reservoirs, diversion structures, canals, laterals, and aquaducts have all irrevocably changed the habitat of the woundfin. The resultant stream dewatering and the return of physically, chemically, and biologically (i.e., exotic species) polluted water to the main channel have destroyed or markedly changed southwestern rivers formerly inhabited by the woundfin. Increasing human populations and increased demands for water will increase pressures on the woundfin unless measures are implemented to protect its habitat (02). Examination of field notes and collections at the Univ. of Nevada, Las Vegas, Brigham Young Univ., Univ. of Michigan Museum of Zoology, and U.S. National Museum indicate the abundance of woundfin in the Virgin River above Mesquite, Nevada. Today the woundfin is rarely found below Mesquite. The red shiner (Notropis lutrensis) has replaced the woundfin in the modified habitats in the lowermost reaches of the river (02) and has recently become established in the upper reaches of the Virgin River. In 1984, red shiners were captured for the first time in the Virgin River above the gorge. By 1985 they comprised 25 percent of the fish taken in the St. George area. Still, populations in the upper Virgin River are large and self- sustaining. Much of the detail of the biology of the species is remains unknown. At times, 100 or more individuals are caught in one seining, while at other times none are caught (10). The present extent of the species population is all that is left of a formerly large range that probably included most of the rivers of Arizona. The danger lies in that the entire population of the species is in one small river system and could be lost in one major catastrophe or slow negative change in the habitat. It is, therefore, essential that at least 2 additional populations of woundfin be established and maintained in suitable areas within their probable historic range (02). SPECIES INTERRELATIONSHIPS: The red shiner (Notropis lutrensis) largely replaces woundfin where they occur together in modified reaches of the Virgin River. Red shiners occur sympatrically with woundfin near Littlefield, Arizona, where shiners are rare and woundfin are usually common. The habitat here is relatively undisturbed. These data suggests that woundfin maintain a competitive edge over the introduced red shiner in unmodified habitat, or may indicate habitat preferences. Predators of woundfin include piscivorous birds such as kingfishers and herons, soft-shelled turtles and other vertebrate species. Woundfin are especially vulnerable during periods of low flow and clear water. Fish that feed on woundfin doubtlessly include Life History - 3 (DRAFT) - Life History Species WOUNDFIN Species Id ESIS252001 Date 14 MAR 96 Gila robusta seminuda (feeding on all life stages), and Lepidomeda m. mollispinis (feeding on larvae and fry). The exotic largemouth bass (Micropterus salmoides) and green sunfish (Lepomis cyanellus) population in the Virgin River is small. Channel catfish (Ictalurus punctatus), while rare in the Virgin River system, may prey on all life stages of the woundfin (02). OTHER LIFE HISTORY DESCRIPTORS: None. Life History - 4
                           (DRAFT) - Management Practices
                                  Species WOUNDFIN
                                Species Id ESIS252001
                                   Date 14 MAR 96



MANAGEMENT PRACTICES

RESULT MANAGEMENT PRACTICE Beneficial Maintaining/Controlling Water Flow Beneficial Maintaining undisturbed/undeveloped areas Beneficial Land Acquisition Beneficial Water Right Acquisition Beneficial Controlling pollution [thermal, chemical, physical] Beneficial Controlling/Restricting Agricultural Practice Beneficial Stocking captive-reared wild-strain animals Beneficial Transplanting wild animals Beneficial Controlling/Removing Exotic Vertebrates Adverse Siltation Existing Siltation Adverse Salinity alteration Existing Salinity alteration Adverse Irrigating Existing Irrigating Adverse Reservoirs Existing Reservoirs Adverse Migration barriers Existing Migration barriers Adverse Environmental Contamination/Pollution Existing Environmental Contamination/Pollution Adverse Exotic/Feral/Introducted Species Existing Exotic/Feral/Introducted Species COMMENTS ON MANAGEMENT PRACTICES - The major reason for the Endangered status of the woundfin is the man-made dams on the rivers where it formerly lived. There are now 8 major dams on the Salt, Verde, and Gila Rivers. These have effectively cut off natural flow in the lower portions of these streams. Suitable habitat may yet exist above San Carlos Reservoir (Gila) and Horseshoe Reservoir (Verde). Lake Mead inundated the lower parts of the Virgin and Moapa Rivers. In addition, habitat alterations resulting from irrigation diversions have reduced the suitability of portions of the Virgin River. Areas of concern include the river from Mesquite, NV to Lake Mead, and the 1-2 kilometers below the Washington-St George Canal Company diversion near St. George (02). A unquantified problem is competition with introduced species. Woundfin are intimately associated with only 4 native and 1 introduced fishes, though 8 other introduced and 2 other native fishes are known from the Virgin River system. The high salinity and turbidity of the Virgin may now be giving a competitive advantage to the woundfin, but if water quality is improved, the introductions may gain the competitive edge. Introductions may have out-competed woundfin in other rivers in its former range (10). Another unquantified factor may be pollution by chemicals such as pesticides, herbicides, and fertilizers added to the river from returning irrigation waters (10). Plans exist for as many as 9 more water projects on the Virgin River (11). Management Practices - 1 (DRAFT) - Management Practices Species WOUNDFIN Species Id ESIS252001 Date 14 MAR 96 APPROVED PLAN: U.S. Fish and Wildlife Service. 1985. Revised Recovery Plan for the Woundfin Plagopterus argentissimus Cope. U.S. Fish and Wildlife Service, Albuquerque, NM. 74 pp. The revised Recovery Plan calls for maintaining and enhancing existing woundfin populations through: monitoring the species and habitat; studying habitat requirements; protecting habitat through cooperative management agreements; habitat management planning; identifying and implementing habitat enhancement features; watershed vegetation management; controlling exotic fishes once studies are under taken to determine the nature and extent of interactions; and maintaining optimum habitat including water flows. The plan also calls for the restoration of woundfin to their former range (where suitable habitat still exists), locating potential habitat, selecting and enhancing transplant sites, and undertaking such transplants. Finally, it suggests a good public education program and continuing law enforcement activities. The danger lies in that the entire population of the species is in one small river system and could be lost in one major catastrophe or slow negative change in the habitat. It is therefore essential that at least 2 additional populations of woundfin be established and maintained in suitable areas within their probable historic range (02). In 1986, the USFWS reintroduced the woundfin into the Gila River drainage of Arizona (specific locations can be found in the occurrence narrative). These populations are determined as "nonessential experimental" populations of an Endangered species to be treated as if they were Threatened. In the past, this species was more widespread in the State of Arizona where it occurred in several river drainages. Introductions are being taken in an effort to reestablish populations of woundfin within their historic range. As of 1986, plans call for annual stocking for the next ten years. Management Practices - 2
                                   (DRAFT) - References
                                     Species WOUNDFIN
                                  Species Id ESIS252001
                                      Date 14 MAR 96



     

References

*****REFERENCES FOR ALL NARRATIVES EXCEPT N-OCCURRENCE***** 01 Miller, R.R. and C.L. Hubbs. 1960. The Spiny-Rayed Cyprinid Fishes (Plagopterini) of the Colorado River System. Misc. Publ. Mus. Zool. Univ. Mich. 115:1-39. 02 U.S. Fish and Wildlife Service. 1985. Recovery Plan for the Woundfin, Plagopterus argentissimus Cope. 74 pp. 03 Hubbs, C.L. 1955. Hybridization Between Fish Species in Nature. Syst. Zool. 4:1-20. 04 Cope, E.D. 1874. On The Plagopterinae and the Icthyology of Utah. Amer. Philos. Soc., Proc. 14:129-140. 05 Jordan, D.S. and C.H. Gilbert. 1883. Synopsis of the Fishes of North America. Bull. U.S. Natl. Mus. 16:I-LVI + 1-1018. 06 Jordan, D.S. and B.W. Evermann. 1896. The Fishes of North and Middle America. Bull. U.S. Natl. Mus., Part I, 47:I + LX, 1-1240. 07 Jordan, D.S., B.W. Evermann, and H.W. Clark. 1930. Checklist of the Fishes and Fish-Like Vertebrates of North and Middle America North of the Northern Boundary of Venezuela and Columbia. U.S. Fish Comm. Rept. (1928). Doc. No. 1055. 670 pp. 08 Tanner, V.M. 1936. A Study of the Fishes of Utah. Proc. Utah Acad. Sci., Arts, Lett.; 13:155-178. 09 Bohlke, J. 1953. A Catalogue of the Type Specimens of Recent Fishes in the Natural History Museum of Stanford University. Stanford Ichthy. Bull., 5:1-168. 10 Burton, J. 1983. Personal Communications. USFWS, P.O. Box 1306, Albuquerque, NM 87103. 11 Hickman, T. 1983. Personal Communication. USFWS, P.O. Box 1575, St. George, UT 84770. 12 Hardy, T.B. and J.E. Deacon. 1985. Impact analysis of the proposed Quail Creek Reservoir on Plagopterus argentissimus (woundfin) in the Virgin River. Unpubl. Bio. Assessment for the Washington County Water Conservation District. 43 pp. 13 Lockhart, J.N. 1977. Personal Communication Cited in 02 above. 14 Deacon, J.E. 1979. Endangered and Threatened Fish of the West. Great Basin Nat. 39:41-64. 15 Deacon, J.E. and T.B. Hardy. 1982. Streamflow Requirements of Woundfin (Plagopterus argentissimus) in the Virgin River, Utah, Arizona, and Nevada. Typescript, 21 pp. 16 Lockhart, J.N. 1979. Ecology of the Woundfin Minnow, Plagopterus argentissimus, Cope. M.S. Thesis, Univ Nevada, Las Vegas. 187 pp. 17 Winget, R.N. and R.W. Baumann. 1977. Virgin River, Utah-Arizona- Nevada, Aquatic Habitat, Fisheries and Microinvertebrate Studies; in: Impact of Warner Valley Water Project on Endangered Fish of the Virgin River. Vaughn Hansen Associates, Salt Lake City, Utah. 18 Cope, E.D. and H.C. Yarrow. 1875. Report upon the Collections of Fishes Made in Portions of Nevada, Utah, California, Colorado, New Mexico, and Arizona, During the Years 1871, 1872, 1873, and 1874. Rept. Geol. Expl. Surv. W 100th Meridian (Wheeler Survey), 5:635- 703. 19 Odum, W.E. 1970. Utilization of the Direct Grazing and Plant Detritus Food Chains by the Striped Mullet Mugil cephalus, P. References - 1 (DRAFT) - References Species WOUNDFIN Species Id ESIS252001 Date 14 MAR 96 222-240; In: J.H. Steele (ed.), Marine Food Chains. Univ Calif. Press, Berkeley. 20 Gregor, P.D. and J.E. Deacon. 1982. Food Partitioning of the Fishes in the Virgin River. Typescript, 110 pp. 21 Deacon, J.E. 1979. Unpublished Data Cited in 02 above. 22 Deacon, J.E. 1977. Habitat Requirements of Woundfin in the Virgin River in Relation to the Proposed Warner Valley Project; In: Impact of Warner Valley Water Project on Endangered Fish of the Virgin River. Vaughn Hansen Associates, Salt Lake City, Utah. 23 Gregor, P.D. and J.E. Deacon. 1982. Some Observations on Woundfin in an Outdoor Experimental Stream. Great Basin Nat. 42 pp. 24 Peters, E.J. 1970. Changes with Growth in Selected Body Proportions of the Woundfin (Plagopterus argentissimus Cope: Cyprinidae). M.S. Thesis, Brigham Young Univ, Provo, Utah. 25 Lockhart, J. 1975. Studies of the Woundfin, Plagopterus argentissimus. In: Proc. Desert Fishes Council (1983) III-IX: 173 pp. 26 Deacon, J.E. 1980. Effects of the Low Flow on Woundfin in the Virgin River. In: Proc. Desert Fishes Council (1980)XII:79 pp. 27 La Rivers, IRA. 1962. Fishes and Fisheries of Nevada. Nevada State Fish and Game Comm. State Printing Office, Carson City, NV. 782 pp. *****REFERENCES FOR N-OCCURRENCE NARRATIVE ONLY***** 01 U.S. Fish and Wildlife Service. 1985. Recovery Plan for the Woundfin, Plagopterus argentissimus Cope. 74 pp. 02 Burton, J. 1983. Personal Communication. USFWS, P.O. Box 1306, Albuquerque, NM 87103. 03 Deacon, J.E. and W.G. Bradley. 1972. Ecological Distribution of Fishes of Moapa River in Clark County, Nevada. Trans. Amer. Fish. Soc. 101:408-419. 04 Gilbert, C.H. and N.B. Scofield. 1898. Notes on a Collection of Fishes from the Colorado Basin in Arizona. Proc. U.S. Nat. Mus. 20:487-499. 05 Jordan, D.S. and B.W. Evermann. 1896. The Fishes of North and Middle America. Bull. U.S. Nat. Mus. 16:I-LVI + 1-1240 (appropriate heading; "Fort Yuma"). 06 USDI/USFWS. 1984. Proposed Determination of Experimental Population Status for Certain Introduced Populations of Colorado Squawfish and Woundfin. Federal Register 49: 14149/14152; April 10, 1984. 07 Deason, W. 1973. Status of Woundfin (Plagopterus argentissimus) Recovery Plans. In: Proc. Desert Fishes Council (1983) III-IX:105. References - 2