(DRAFT) - Taxonomy
                            Species GAMBUSIA, CLEAR CREEK
                                Species Id ESIS252005
                                   Date 14 MAR 96



TAXONOMY

NAME - GAMBUSIA, CLEAR CREEK OTHER COMMON NAMES - GAMBUSIA and CLEAR CREEK ELEMENT CODE - CATEGORY - Fish PHYLUM AND SUBPHYLUM - CHORDATA, CLASS AND SUBCLASS - OSTEICHTHYES, ORDER AND SUBORDER - ATHERINIFORMES, FAMILY AND SUBFAMILY - POECILIIDAE, GENUS AND SUBGENUS - GAMBUSIA, SPECIES AND SSP - HETEROCHIR, SCIENTIFIC NAME - GAMBUSIA HETEROCHIR AUTHORITY - TAXONOMY REFERENCES - COMMENTS ON TAXONOMY - Clear Creek Gambusia Gambusia heterochir Hubbs, 1953 KINGDOM: Animal GROUP: Fish PHYLUM: Chordata CLASS: Osteichthyes ORDER: Atheriniformes FAMILY: Poeciliidae The Clear Creek gambusia (Gambusia heterochir Hubbs, 1953) is a stocky Poeciliid with a metalic blue sheen. A series of crescentic dark marks on the posterior margin of lateral scales from a crosshatched pattern. There is no predorsal dark streak or caudal speckling. Females have a pronounced darkening around the anus. A deep notch on the dorsal margin of the pectoral fin of adult males is unique among Poeciliids. The gonopodium is blunt with short spines on ray 3, a pronounced elbow and separate distal elements on ray 4A, a simple terminal hook on 4P, and a rounded terminal hook on ray 5. The Clear Creek gambusia (Gambusia heterochir) once was considered to be a member of the Gambusia nicarguensis species group (01) but is now generally considered to be a member of the G. nobilis species group (02,03,04,05,06,07). The holotype and several paratypes are deposited in the University of Michigan Museum of Zoology. Most specimens are Taxonomy - 1 (DRAFT) - Taxonomy Species GAMBUSIA, CLEAR CREEK Species Id ESIS252005 Date 14 MAR 96 deposited at the Texas Natural History Collection. Illustrations may be found in (01) and in (08). Taxonomy - 2
                                  (DRAFT) - Status
                            Species GAMBUSIA, CLEAR CREEK
                                Species Id ESIS252005
                                   Date 14 MAR 96



STATUS

Coded Status E: Federal Endangered COMMENTS ON STATUS - U.S. STATUSES AND LAWS: The Clear Creek gambusia (Gambusia heterochir) has been designated an Endangered species pursuant to the Endangered Species Act of 1973 (50 CFR 17.11; P.L. 93-205, 87 Stat. 884; 16 U.S.C. 1531-1540), as amended. The species has this status wherever found including the State of Texas. This species is protected by the Lacey Act (P.L. 97-79, as amended; 16 U.S.C. 3371 et seq.) which makes it unlawful to import, export, transport, sell, receive, acquire, or purchase any wild animal (alive or dead including parts, products, eggs, or offspring): (1) in interstate or foreign commerce if taken, possessed, transported or sold in violation of any State law or regulation; or (2) if taken or possessed in violation of any U.S. law, treaty, or regulation or in violation of Indian tribal law. It is also unlawful to possess any wild animal (alive or dead including parts, products, eggs, and offspring) within the U.S. territorial or special maritime jurisdiction (as defined in 18 U.S.C. 7) that is taken, possessed, transported, or sold in violation of any State law or regulation, foreign law, or Indian tribal law. RESPONSIBLE FEDERAL AGENCIES: USFWS -Responsible for the management/recovery, listing, and law enforcement/protection of this species. All Federal agencies have responsibility to ensure that any action authorized, funded, or carried out by that agency is not likely to jeopardize the continued existence of the species or result in the destruction or adverse modification of Critical Habitat (50 CFR 402), and to utilize their authorities to carry out programs for the conservation of the species. STATE STATUSES AND LAWS: STATE: Texas DESIGNATED STATUS: Endangered ADMINISTRATIVE AGENCY: Texas Parks and Wildlife Department STATE STATUTE: 31 Texas Administrative Code Sec. 57.133 (127.30. 003) July 1977 Status - 1 (DRAFT) - Status Species GAMBUSIA, CLEAR CREEK Species Id ESIS252005 Date 14 MAR 96 INTERNATIONAL STATUSES, TREATIES, AND AGREEMENTS: The Clear Creek gambusia is listed in the IUCN Red Data Book, Vol. 4, 1977; and is also listed by the Convention on Nature Protection and Wildlife Preservation in the Western Hemisphere, 1970. ECONOMIC STATUSES: This species has value to naturalists and has a value as a component of Texas' natural heritage of biotic diversity. 67/03/11:32 FR 04001/ - Listing as Endangered 79/05/21:44 FR 29565/29577 - Five year review 85/07/22:50 FR 29901/29909 - Five year review Status - 2
     

HABITAT ASSOCIATIONS

HABITAT - AQUATIC INLAND AQUATIC LAND USE - Shrub and Brush Rangeland Streams and Canals Reservoirs NATIONAL WETLAND INVENTORY CODES NWI NWICLS NWIMOD NWISPEC Lacustrine, littoral AB4 COMMENTS ON HABITAT ASSOCIATIONS - The Clear Creek gambusia (Gambusia heterochir) occurs in the headwaters of Clear Creek, Menard County, Texas, 16.7 km west of Menard. Upper Clear Creek consists of a series of limestone springs (Wilkinson Springs) (07) at the base of a limestone cliff (06); with the springs originating from the Edwards Aquifer (07). The watershed emptying into Clear Creek is extremely limited encompassing about 10 hectares (07), forming intermittent creeks each about 1 km long (with 1 square kilometer basins) and emptying into Clear Creek downstream of the head pool (07). Originally Clear Creek was a clear spring run that flowed about 5 km to its confluence with the San Saba River. Most or all the stream was probably inhabited by G. heterochir, Hyalella texana (amphipod), Ceratophyllum sp. and associated organisms (07). Presently Clear Creek has an elevational diference of about 15 m from headspring to confluence. Dam 1, built before 1890, forms the present head pool. Dam 2 is constructed 1.5 km downstream from Dam 1 and backs water to the base of Dam 1. Dam 3 (approximately the same elevation as Dam 2) was constructed 1.5 km downstream of Dam 2 and Dam 4 was built 0.4 km downstream of Dam 3 and 100 m upstream of U.S. Highway 190. The resulting changes in habitat encourage population build-ups of eurythermal organisms overwhelming the springrun fauna except for those isolated upstream of Dam 1 (07). The Clear Creek gambusia occurs in stenothermal (ca 20 degrees C) spring environments with dense growths of Cerotophyllum and Ludwegia. A relatively low pH (ca 6.5) is positively associated with Clear Creek gambusia abundance (06). Reduction in spring flow and/or riparian development activities (e.g., resort housing) that drain, silt, and/or introduce pesticides/herbicides into the spring pool are the primary hazards (07). Some of these factors could also cause eutrophic conditions. The Clear Creek gambusia habitat is obviously dependent upon a reliable and substantial supply of spring water; therefore, it is essential that the Edwards-Trinity Aquifer recharge zone remain undisturbed. Most of the local recharge zone appears to be in ranchland north and west of the headspring. Surrounding shrub/brush rangeland is classified as Texas savanna, and usage is primarily ranchland. Habitat Associations - 1
                                (DRAFT) - Food Habits
                            Species GAMBUSIA, CLEAR CREEK
                                Species Id ESIS252005
                                   Date 14 MAR 96



FOOD HABITS

TROPHIC LEVEL - CARNIVORE LIFESTAGE FOOD FOOD PART General Zooplankton General Arthropods General Worms General Fish General Crustaceans Food Habits - 1
                         (DRAFT) - Environment Associations
                            Species GAMBUSIA, CLEAR CREEK
                                Species Id ESIS252005
                                   Date 14 MAR 96



ENVIRONMENTAL ASSOCIATIONS

G = General A = Adult LIM = Limiting RA = Resting Adult J = Juvenile FA = Feeding Adult RJ = Resting Juvenile BA = Breeding Adult FJ = Feeding Juvenile P = Pupae L = Larvae E = Egg RL = Resting Larvae FL = Feeding Larvae
LIFESTAGE ENVIRONMENTAL ASSOCIATIONS G Aquatic Features: Springs [flowing] G Terrestrial Features: Cliffs/ledges Environment Associations - 1
                               (DRAFT) - Life History
                            Species GAMBUSIA, CLEAR CREEK
                                Species Id ESIS252005
                                   Date 14 MAR 96



LIFE HISTORY

FOOD HABITS: The primary food eaten by adults is the amphipod, Hyalella texana (80 percent) (06,12). It is presumed that small Gambusia eat small Hyalella. In culture the Clear Creek gambusia eats a wide variety of foods (13) and would presumably do so in nature if available. HOME RANGE/TERRITORY: Males are semiterritorial during the breeding season - alpha males occupy "preferred habitat"; omega males occupy "suboptimal habitat " (vulnerable to predation and having fewer females). Females abound in preferred habitat (06). PERIODICITY: Diurnal - activity peaks at 0800 and 1800 (06). The species is active in all seasons (the water is stenothermal). MIGRATION PATTERNS: No migrations are known for the Clear Creek gambusia (Gambusia heterochir). COVER/SHELTER REQUIREMENTS: G. heterochir is most abundant in shallow waters with dense growths of Ceratophyllum and Ludwegia (01,06,09). REPRODUCTIVE SITE REQUIREMENTS: Nothing is known for the Clear Creek gambusia regarding reproductive site requirements. REPRODUCTIVE CHARACTERISTICS: This species is viviparous (a live bearer) with 2 generations per year and seldom lives more than one year in nature, but may live more than 2 years in the laboratory. Females reproduce in February- October; have 6 broods per year; are promiscuous with internal fertilization (sperm may be stored several months); and multiple inseminations (per brood) may occur. Gestation is 40 days. Clutch size is dependent upon size of female, and up to 100 young may be produced per brood. Relative brood numbers are seasonally dependant with a maximum in August, but high in May-September (06). PARENTAL CARE: No parental care is known. POPULATION BIOLOGY: Occurs in stenothermal waters in Clear Creek, unable to coexist with Gambusia affinis in eurythermal waters. Present population densities are over 1/square meter in optimal environments. SPECIES INTERRELATIONSHIPS: The Clear Creek gambusia (Gambusia heterochir) primarily feeds on Hyalella texana (an amphipod) (06,12). This species does not coexist with G. affinis in eurythermal environments (06). G. heterochir Life History - 1 (DRAFT) - Life History Species GAMBUSIA, CLEAR CREEK Species Id ESIS252005 Date 14 MAR 96 hybridizes with G. affinis (06,11). An apparently recent introduction to Clear Creek is the rainwater killifish (Lucania parva), first collected in 1980. The rainwater killifish is a brackishwater species formerly known only from coastal waters and the Pecos River, but is expected to reproduce in Clear Creek. If the killifish reproduces and becomes established in Clear Creek, its impact on G. heterochir cannot be predicted. Both Lucania and G. heterochir occupy similar niches and are likely to compete for food and cover (07). A variety of potential predators which presumably feed on gambusia have been recorded in Clear Creek. These include the largemouth bass (Micropterus salmoides), green sunfish (Lepomis cyanellus), longear sunfish (Lepomis megalotis), exotic redear sunfish (Lepomis microlophus), and yellow bullhead (Ictalurus natalis). Semi-aquatic snakes (Nerodia sp. and Thamnophis sp.) and bullfrogs (Rana catesbeiana) have been seen along shore and likely feed on gambusia. These piscivorous vertebrates have a long history of occurrence in central Texas and their predation would be a normal selective factor on G. heterochir. Unless the habitat is seriously altered (e.g., removal of aquatic plants), the predatory activities of these vertebrates are not expected to deplete the gambusia seriously (07). OTHER LIFE HISTORY DESCRIPTORS: Clear Creek gambusia cohabit the upper pool with the roundnose minnow (Dionda episcopa) and the greenthroat darter (Etheostoma lepidum). These small fishes have minimal environmental overlap. Dionda is a midwater fish with omnivorous food habits; Etheostoma and gambusia feed on small invertebrates such as Hyalella, but Etheostoma lives on the substrate and gambusia lives near the surface. Two anurans (Acris crepitans and Rana berlandieri) occupy the shores of the upper pool and may occasionally feed on aquatic invertebrates on the surface. Their tadpoles inhabit the shores of the upper pool, but are herbivores. Life History - 2
                           (DRAFT) - Management Practices
                            Species GAMBUSIA, CLEAR CREEK
                                Species Id ESIS252005
                                   Date 14 MAR 96



MANAGEMENT PRACTICES

RESULT MANAGEMENT PRACTICE Beneficial Maintaining/Controlling Water Flow Beneficial Developing/maintaining/protecting wetlands Beneficial Maintaining undisturbed/undeveloped areas Beneficial Land Acquisition Beneficial Water Right Acquisition Beneficial Controlling/Restricting Pesticide Use Beneficial Controlling/Restricting Herbicide Use Beneficial Reforestation Beneficial Stocking captive-reared wild-strain animals Beneficial Controlling/Removing Exotic Vertebrates Adverse Low Gene Pool Existing Low Gene Pool Adverse Hybridization Existing Hybridization Adverse Water Temperature Alteration Existing Water Temperature Alteration Adverse Water Level Stabilization Existing Water Level Stabilization Adverse Flooding Existing Flooding Adverse Groundwater drawdown Existing Groundwater drawdown Adverse Reservoirs Existing Reservoirs Adverse Migration barriers Existing Migration barriers Adverse Shoreline modification/development Existing Shoreline modification/development Adverse Applying herbicides Existing Applying herbicides Adverse Applying pesticides Existing Applying pesticides Adverse Competition Existing Competition Adverse Exotic/Feral/Introducted Species Existing Exotic/Feral/Introducted Species Adverse Existing COMMENTS ON MANAGEMENT PRACTICES - Upper Clear Creek has been altered extensively for irrigation and domestic uses. Prior to 1900, a low, earth-concrete dam was built about 75 meters downstream from the headsprings. Three additional dams were built downstream from the original dam in the 1930's, ponding water to the base of each subsequent dam (06) and flooding previously irrigated fields (07). The Clear Creek gambusia (Gambusia heterochir) is a spring dwelling species, limited to stenothermal headsprings in Clear Creek, Management Practices - 1 (DRAFT) - Management Practices Species GAMBUSIA, CLEAR CREEK Species Id ESIS252005 Date 14 MAR 96 Menard County, TX. This ecosystem is unique in having a relatively low pH (ca. 6.5), with the presence of Hyalella texana (an amphipod) and a species of Ceratophyllum. Adjacent eurythermal ponded waters are dominated by a relatively high pH (7.4-7.8), Hyalella azteca and a species of Myriophyllum (09). The eurythermal environment is inhabited by Gambusia affinis and no self-sustaining Gambusia heterochir populations exist there. Virtually the entire length of Clear Creek is impounded (07) and only areas around springs have sufficient inflow to maintain the stenothermal environment. The vast majority of the stenothermal surface area is in the upper pool (100 x 70 m - triangular) (06,09) and a secondary spring area (20 x 40 m) is located 250 meters east and had virtually no Gambusia heterochir prior to 1970. Subsequent to the release of the exotic fish; Lucinia parva, presumbaly from Texas coastal waters (10), Gambusia heterochir became more abundant than G. affinis (11). A minor and presumably not self-sustaining population occurs in a spring area on west bank, 50 meters south of the head spring pool. At one time a population also existed on the west bank at another spring 25 meters south of the spring pool (09). The presumed major cause for the restricted distribution of the Clear Creek gambusia was the construction of an earth dam, 3 km east of the head springs (07). That dam backs water (eurythermal) to the base of the dam forming the headspring pool. This established an environment more suitable for G. affinis and a substantial reduction in the range from 3 kilometers of stream to the present two ponded regions. The environmental limitations are exacerbated by hybridization with G. affinis. This hazard was substantial in the upper spring pool due to breaches in the dam, prior to 1979 (06). In 1979 the spring pool dam was reconstructed to preclude immigration by G. affinis. Hybrid abundance was reduced from 10 percent of the Gambusia prior to 1970 to 5 percent in 1980 and 1 percent in 1984. Between 1980 and 1984 G. affinis abundance declined from 30 to 20 percent; the Clear Creek gambusia abundance went from 60 percent in 1970 to 65 percent in 1980 to 75 percent in 1984, presumably as a result of the blocking of immigration by G. affinis (11). Although the present abundance is much greater than in 1970 (and especially in 1978 when the upper dam was about to collapse entirely) the geographic range of the Clear Creek gambusia is very restricted. Although G. heterochir can be maintained in a variety of environments in the laboratory, the restriction to stenothermal waters in nature suggests a biological factor, perhaps competition, as the major factor limiting distribution (07). Any alteration (i.e., groundwater drawdown) to the aquifer or the immediate surroundings of the headspring pool (the ranch was sold in 1984) could have disasterous results. Reduction in spring flow and/or riparian development activities (e.g., resort housing) that drain, silt, and/or introduce pesticides/herbicides into the spring pool are the primary hazards (07). Some of these factors could also cause eutrophic conditions. Any development in the area that would result in removal of the earth-concrete dam would permit invasion by G. affinis thus eliminating G. heterochir (07). The Clear Creek gambusia habitat is obviously dependent upon a Management Practices - 2 (DRAFT) - Management Practices Species GAMBUSIA, CLEAR CREEK Species Id ESIS252005 Date 14 MAR 96 reliable and substantial supply of spring water; therefore, it is essential that the Edwards-Trinity Aquifer recharge zone remain undisturbed. Most of the local recharge zone appears to be in ranchland north and west of the headspring. APPROVED PLAN: U.S. Fish and Wildlife Service. 1982. Clear Creak Gambusia Recovery Plan. U.S. Fish and Wildlife Service, Albuquerque, New Mexico. 29 pp. The primary objective of the Recovery Plan is to improve the status of the Clear Creek gambusia (Gambusia heterochir) so that it may continue to survive in and near the headsprings of Clear Creek, Menard County, TX. The principle concern of the plan was that, since the area is privately owned and was for sale at the time of writing (1980), the USFWS acquire management rights to the gambusia habitat. This was not accomplished before the sale. If a similar opportunity arises in the future, it may be desirable to aquire the land and/or water rights. Fortunately, the owner has been very helpful and concerned about the gambusia and management of the springs for the species benefit continues. Protection of the habitat including the headspring pool and aquifer recharge zone (maintaining waterflow and controlling development) is important due to the limited distribution of the Clear Creek gambusia. If the entire stream is also protected it may be beneficial to revert the entire 5 km of stream back into a spring run habitat by removing dams. The extensive exposed mudflats that would be left by dam removal should be seeded to prevent erosion. Understanding the biology and ecology of the Clear Creek gambusia is also a high priority. Recommended research includes: 1) Studies of competition between the G. heterochir and G. affinis. 2) Studies of prey species of the G. heterochir. 3) Studies of reproductive variables. 4) Studies of predation on the G. heterochir. 5) Studies of survivorship. 6) Studies of diseases and parasites. Since maintenance and enhancement of existing habitat is critical to the species survival; the physical, chemical, and biological features of the habitat should be studied in relation to species abundance. The controlling of herbicide and pesticides known to be detrimental to this species in the immediate area and the restricting of development may help maintain the integrity of the habitat. Management of Clear Creek above Dam 1 requires maintaining its integrity to stop further immigration of G. affinis into Clear Creek gambusia habitat. Repairs to Dam 1 in 1979, which again separated the majority of the G. affinis population from the G. heterochir habitat, was an important step in this process. Current plans by the new owner to reconstruct Dam 1 with USFWS technical assistance are encouraging. These modifications and management steps will help to maintain the water flow. Management for Clear Creek gambusia should also include monitoring populations 2 to 4 times annually. Captive populations should be maintained at Dexter NFH for scientific research and Management Practices - 3 (DRAFT) - Management Practices Species GAMBUSIA, CLEAR CREEK Species Id ESIS252005 Date 14 MAR 96 reintroduction. The Clear Creek gambusia should be provided the full protection mandated by Federal and Texas laws. Finally, public awareness of the uniqueness of the Clear Creek gambusia should be increased through the preparation of information pamplets and news releases. Management Practices - 4
                                   (DRAFT) - References
                              Species GAMBUSIA, CLEAR CREEK
                                  Species Id ESIS252005
                                      Date 14 MAR 96



     

References

***** REFERENCES FOR ALL NARRATIVES EXCEPT N-OCCURRENCE ***** 01 Hubbs, C. 1957. Gambusia heterochir, a new Poeciliid fish from Texas with an account of its hybridization with Gambusia affinis. Tulane Stud. 5:1-16. 02 Minckley, W.L. 1962. Two new species of fishes of the genus Gambusia (Pieciliidae) from northwestern Mexico. Copeia 1962:391-396. 03 Rosen, D.E. and R.M. Bailey. 1963. The Poeciliid fishes (Cyprinodontiformes), their structure, zoogeography, and systematics. Bull. Amer. Mus. Nat. Hist. 126:1-176. 04 Rivas, L.R. 1963. Subgenera and species groups in the Poeciliid fish genus Gambusia Poey. Copeia 1963:331-347. 05 Peden, A.E. 1973. Variation in anal spot expression of gambusine females and its effect on male courtship. Copeia 1973:250-263. 06 Hubbs, C. 1971. Competition and isolation mechanisms in the Gambusia affinis x G. heterochir hybrid swarm. Bull. TX Mem. Mus. 19:47 pp. 07 U.S. Fish and Wildlife Service. 1980. Clear Creek Gambusia (Gambusia heterochir) Recovery Plan. U.S. Fish and Wildlife Service, Albuquerque, NM. 29 pp. 08 Rhode, F.C. 1980. Gambusia heterochir Hubbs Clear Creek gambusia. In: Atlas of North American freshwater fishes. D.S. Lee, et al. 543 pp. 09 Hubbs, C. 1959. Population analysis of a hybrid swarm between Gambusia affinis and Gambusia heterochir. Evolution 13:236-246. 10 Hubbs, C. 1982. Occurrence of exotic fishes in Texas waters. Pearce-Sollards Series, TX Mem. Mus. 36. 19 pp. 11 Edwards, R.J. and C. Hubbs. In press. Temporal changes in the Gambusia heterochir x G. affinis hybrid swarm following dam reconstruction. Endangered Sp. Rep. USFWS, Albuquerque, NM. 12 Stevenson, M.M. and A.E. Peden. 1973. Description and ecology of Hyalella texana n. sp. (Crustacea:Amphipoda) from the Edwards Plateau of Texas. Am. Midl. Nat. 89:426-436. ***** REFERENCES FOR N-OCCURRENCE NARRATIVE ONLY ***** 01 Hubbs, C. 1971. Competition and isolation mechanisms in the Gambusia affinis x G. heterochir hybrid swarm. Bull. TX Mem. Mus. 19:47 pp. 02 Edwards, R.J. and C. Hubbs. In press. Temporal changes in the Gambusia heterochir x G. affinis hybrid swarm following dam reconstruction. Endangered Sp. Rep. USFWS. Albuquerque, NM. 03 U.S. Fish and Wildlife Service. 1980. Clear Creek Gambusia (Gambusia heterochir) Recovery Plan. U.S. Fish and Wildlife Service, Albuquerque, NM. 29 pp. References - 1