(DRAFT) - Taxonomy
                               Species GAMBUSIA, PECOS
                                Species Id ESIS252006
                                   Date 14 MAR 96



TAXONOMY

NAME - GAMBUSIA, PECOS OTHER COMMON NAMES - GAMBUSIA and PECOS ELEMENT CODE - CATEGORY - Fish PHYLUM AND SUBPHYLUM - CHORDATA, CLASS AND SUBCLASS - OSTEICHTHYES, ORDER AND SUBORDER - ATHERINIFORMES, FAMILY AND SUBFAMILY - POECILIIDAE, GENUS AND SUBGENUS - GAMBUSIA, SPECIES AND SSP - NOBILIS, SCIENTIFIC NAME - GAMBUSIA NOBILIS AUTHORITY - TAXONOMY REFERENCES - COMMENTS ON TAXONOMY - Pecos Gambusia Gambusia nobilis (Baird and Girard, 1853) KINGDOM: Animal GROUP: Fish PHYLUM: Chordata CLASS: Osteichthyes ORDER: Atheriniformes FAMILY: Poeciliidae Gambusia nobilis (Pecos gambusia) is a small, livebearing member of the Poeciliidae. Poeciliids are characterized by strong sexual dimorphism. The anal fin of males is modified into a gonopodium, an intromittent organ used in copulation. Gonopodial structures distinguish G. nobilis from the other poeciliids (i.e., Gambusia affinis and Gambusia geiseri) known to occur within its native range. Spines of ray 3 elongated; hooks on rays 4p and 5a small and rounded; located near terminal end of gonopodium; elbow on ray 4a located opposite the serrae of 4p, composed of 3 or 4 fused segments. Color patterns are useful in making preliminary field identifications and morphometric characters, although environmentally plastic, aid in identification. In profile the back is arched; robust; caudal peduncle, depth approximately two thrids the head length. Margins of scale pockets are outlined in black. The dorsal Taxonomy - 1 (DRAFT) - Taxonomy Species GAMBUSIA, PECOS Species Id ESIS252006 Date 14 MAR 96 fin has a subbasal row of spots. Females have a black area on the abdomen that surrounds the anus and anal fin (01). The caudal fin normally lacks spots but a faint median row may occur (01). The Pecos gambusia is metallic colored; best distinguished by the lack of extensive carotenoid (yellowish) pigmentation. There is substantial differentiation among the isolated populations (02) with the most genetic divergence in the Balmorhea population (03). That difference is sufficient enough that the Balmorhea population could be considered as a distinct subspecies (03). The Pecos gambusia was originally described as Heterandria nobilis (04) but placed in Gambusia in 1859 (05). All subsequent authors have considered Gambusia nobilis to be a valid taxon with the exception of Garman (06), who called it a junior synonym of G. gracilis (Heckel, 1848). A number of authors have confused or synonomized the Pecos gambusia with the Conchos gambusia (G. senilis Girard, 1859) (07,08,25) but, since 1926 (10), the two species have generally been considered as distinct (02,03,10,11,12,13,14). A drawing of a female may be found in the "Atlas of North American Fishes" (15). The lectoholotype is deposited in the Museum of Comparative Zoology (MCZ 1455) as are the lectoparatypes (MCZ 1298). Other specimens are deposited in the U.S. Museum of Natural History, University of Michigan Museum of Zoology, Texas Natural History Collection, University of New Mexico, Tulane University, and the Oklahoma State University. A summary of the taxonomic synonomy and scientific names used in reference to the Pecos gambusia in the literature is as follows: Gambusia nobilis (Baird and Girard) Gambusia gracilis (Heckel) Gambusia senilis (Girard) Gambusia nobilis subsp. Heterandria nobilis (Baird and Girard) Taxonomy - 2
                                  (DRAFT) - Status
                               Species GAMBUSIA, PECOS
                                Species Id ESIS252006
                                   Date 14 MAR 96



STATUS

Coded Status E: Federal Endangered COMMENTS ON STATUS - U.S. STATUSES AND LAWS: The Pecos gambusia (Gambusia nobilis) has been designated an Endangered species pursuant to the Endangered Species Act of 1973 (50 CFR 17.11; P.L. 93-205, 87 Stat. 884; 16 U.S.C. 1531-1540), as amended. The species has this status wherever found including the States of New Mexico and Texas. This species is protected by the Lacey Act (P.L. 97-79, as amended; 16 U.S.C. 3371 et seq.) which makes it unlawful to import, export, transport, sell, receive, acquire, or purchase any wild animal (alive or dead including parts, products, eggs, or offspring): (1) in interstate or foreign commerce if taken, possessed, transported or sold in violation of any State law or regulation; or (2) if taken or possessed in violation of any U.S. law, treaty, or regulation or in violation of Indian tribal law. It is also unlawful to possess any wild animal (alive or dead including parts, products, eggs, and offspring) within the U.S. territorial or special maritime jurisdiction (as defined in 18 U.S.C. 7) that is taken, possessed, transported, or sold in violation of any State law or regulation, foreign law, or Indian tribal law. RESPONSIBLE FEDERAL AGENCIES: USFWS -Responsible for the management/recovery, listing, and law enforcement/protection of this species. All Federal agencies have responsibility to ensure that any action authorized, funded, or carried out by that agency is not likely to jeopardize the continued existence of the species or result in the destruction or adverse modification of Critical Habitat (50 CFR 402), and to utilize their authorities to carry out programs for the conservation of the species. STATE STATUSES AND LAWS: STATE: New Mexico DESIGNATED STATUS: Endangered Group II ADMINISTRATIVE AGENCY: New Mexico Department of Game and Fish Status - 1 (DRAFT) - Status Species GAMBUSIA, PECOS Species Id ESIS252006 Date 14 MAR 96 STATE STATUTE: Regulation No. 624 July 22, 1983 STATE: Texas DESIGNATED STATUS: Endangered ADMINISTRATIVE AGENCY: Texas Parks and Wildlife Department STATE STATUTE: 31 Texas Administrative Code Sec. 57.133 (127.30. 003) July 1977 INTERNATIONAL STATUSES, TREATIES, AND AGREEMENTS: The Pecos gambusia is listed as Vulnerable in the IUCN Red Data Book, Vol. 4, 1977; and is also listed by the Convention on Nature Protection and Wildlife Preservation in the Western Hemisphere, 1970. ECONOMIC STATUSES: This species has value to naturalists and has a value as a component of New Mexico and Texas' natural heritage of biotic diversity. 70/10/13:35 FR 16047/16048 - Listing as Endangered 79/05/21:44 FR 29565/29577 - Five year review 85/07/22:50 FR 29901/29909 - Five year review Status - 2
     

HABITAT ASSOCIATIONS

HABITAT - AQUATIC INLAND AQUATIC LAND USE - Shrub and Brush Rangeland Mixed Rangeland Streams and Canals Lakes Reservoirs NATIONAL WETLAND INVENTORY CODES NWI NWICLS NWIMOD NWISPEC Riverine, upper perennial AB1 Lacustrine, littoral OW0 Lacustrine, littoral AB1 Lacustrine, limnetic OW0 Palustrine EM1 Palustrine AB1 COMMENTS ON HABITAT ASSOCIATIONS - Gambusia nobilis occurs abundantly in springheads and spring runs. Moderately abundant populations are also known from areas with little spring influence, but with abundant overhead cover, sedge covered marshes, and gypsum sinkholes. G. nobilis has been observed to occur from the surface to depths of three meters (01). Occassionally individuals are taken in irrigation ditches especially in the Balmorhea, TX area (02). Gambusia is primarily a subtropical genus. The closest relatives of G. nobilis occur in Mexico and south Texas. For this reason, G. nobilis is known principally from the lower elevations and more thermally stable localities (i.e., springs) within its geographic range. Ink Pot, located on the Salt Creek Wilderness Area northeast of Roswell, represents the highest elevation (approx. 1080 m) and northernmost area presently known to be occupied by this species. All populations, including those at historic, present, and introduction sites, occur between 822 m and 1187 m elevation, a range in elevation of 365 m (01). The narrow elevation range suggests a narrow range of temperature tolerance. Reported average critical thermal maxima is 38.1-39.3 degrees C for G. nobilis, with thermal preferenda of 21-25 degrees C in the morning and 26-30 degrees C in the afternoon. In contrast, the potential competitor G. affinis is more toleratnt of higher temperatures. No data are available on cold tolerances of G. nobilis (01). Gambusia nobilis occurs abundantly in waters with conductivities ranging form near 1200 umhos/cm at Blue Spring to 32,500 umhos/cm at Sinkhole 27 on Bitter Lake National Wildlife Refuge. These conductivity values roughly correspond to total dissolved solids concentrations of 1 and 30 ppt, respectively. Within this range, salinity apparently is not a major limiting factor, although 30 ppt may be near the upper tolerance level of the species. The Pecos gambusias essentially restricted to stenothermal, clear water, lotic habitats (01). It lives in a variety of habitats in Bitter Lake National Wildlife Refuge (21) in the absense of congenors. When sympatric with Gambusia affinis, the Pecos gambusia is restricted to flowing stenothermal waters (01). When sympatric Habitat Associations - 1 with G. geiseri, the Pecos gambusia dominates most stenothermal habitats with salinities of 15,000 umhos/cm, but in salinities of 3500 to 5000 umhos/cm the two species have relatively similar abundances (03). Lands surrounding the habitats are classified as Texas savanna and shrub/brush rangelands of mixed rangelands. Habitat Associations - 2
                                (DRAFT) - Food Habits
                               Species GAMBUSIA, PECOS
                                Species Id ESIS252006
                                   Date 14 MAR 96



FOOD HABITS

TROPHIC LEVEL - CARNIVORE LIFESTAGE FOOD FOOD PART General Algae General Zooplankton General Arthropods General Fish General Crustaceans Food Habits - 1
                         (DRAFT) - Environment Associations
                               Species GAMBUSIA, PECOS
                                Species Id ESIS252006
                                   Date 14 MAR 96



ENVIRONMENTAL ASSOCIATIONS

G = General A = Adult LIM = Limiting RA = Resting Adult J = Juvenile FA = Feeding Adult RJ = Resting Juvenile BA = Breeding Adult FJ = Feeding Juvenile P = Pupae L = Larvae E = Egg RL = Resting Larvae FL = Feeding Larvae
LIFESTAGE ENVIRONMENTAL ASSOCIATIONS G Aquatic Features: Springs [flowing] G Terrestrial Features: Depressions G Environment Associations - 1
                               (DRAFT) - Life History
                               Species GAMBUSIA, PECOS
                                Species Id ESIS252006
                                   Date 14 MAR 96



LIFE HISTORY

FOOD HABITS: The Pecos gambusia like other Gambusia, is considered to be a carnivorous surface feeder (22). It consumes larger fractions of amphpiods than do coexisting fishes (20). Stomachs contain a variety of small invertebrates and will on occassion contain filamentous algae (22). The species seems to be an opportunistic feeder (01). HOME RANGE/TERRITORY: Presumed to be quasi-territorial as is its relative G. heterochir (23). PERIODICITY: The Pecos gambusia is diurnal as are other Gambusia (24). MIGRATION PATTERNS: No information is available on this subject, but, the species is expected to have warm water preference (23). COVER/SHELTER REQUIREMENTS: The Pecos gambusia inhabits stenothermal environments with little cover as well as stenothermal environments with abundant vegetation (01). Moderately abundant populations are also known from areas with little spring influence, but with abundant overhead cover, sedge covered marshes, and gypsum sinkholes. G. nobilis has been observed to occur from the surface to depths of three meters (01). REPRODUCTIVE SITE REQUIREMENTS: Viviparous, parturition primarily in shallow areas (01,,23). REPRODUCTIVE CHARACTERISTICS: Little is known on this subject for this species. At Blue Spring the Pecos gambusia has a mean number of 38 offspring (22); however, congenors have numerous broods each year with substantial seasonal variations in the number of offspring (14). PARENTAL CARE: No parental care is known. POPULATION BIOLOGY: Predation on G. nobilis could be a major limiting factor in areas where no submerged vegetation or sufficiently shallow areas provide cover from predators. Predation by the centrarchids Lepomis cyanellus and/or Micropterus salmoides may have eliminated the introduced population of G. nobilis from Lake St. Francis on the Bitter Lake National Wildlife Refuge and also may have contributed to the failure of a population introduced into Geyser Spring, NM. Also, virtual absence of G. nobilis from the head pool of Diamond-Y Spring may be attributable partly to the presence of L. cyanellus and M. salmoides. Gambusia nobilis is extremely abundant in shallow marshy areas of Leon Creek and Blue Spring, even though predators (centrarchids) are present in the deeper and more open waters (01). Life History - 1 (DRAFT) - Life History Species GAMBUSIA, PECOS Species Id ESIS252006 Date 14 MAR 96 SPECIES INTERRELATIONSHIPS: The Pecos gambusia has a wide range of tolerance to physical/ chemical factors in the water, however in the presence of congenors, the Pecos gambusia is abundant only in special areas. Gambusia affinis is widespread in the range of Gambusia nobilis. G. affinis is tolerant of environmental extremes and where sympatric, G. nobilis is restricted to stenothermal waters (01). At Blue Spring, G. nobilis is very abundant near the head springs but, downstream almost all gambusines are G. affinis. In intermediate reaches, G. nobilis abounds in the spring flow and G. affinis is exceedingly abundant in marginal areas that have substantial thermal oscillation. Gambusia geiseri has been introduced into localities inhabited by G. nobilis (18). At Balmorhea where the spring flows are of low salinity, G. geiseri is the abundant form, but in Leon Creek where the spring flows are of high salinity (ca 1/2 that of sea water) G. nobilis is the more abundant of the two species (03). In Leon Creek, G. affinis predominates downstream and there is a hybrid swarm between G. affinis and G. nobilis at intermediate locations (20). OTHER LIFE HISTORY DESCRIPTORS: None. Life History - 2
                           (DRAFT) - Management Practices
                               Species GAMBUSIA, PECOS
                                Species Id ESIS252006
                                   Date 14 MAR 96



MANAGEMENT PRACTICES

RESULT MANAGEMENT PRACTICE Beneficial Maintaining/Controlling Water Flow Beneficial Controlling water levels Beneficial Land Acquisition Beneficial Water Right Acquisition Beneficial Controlling pollution [thermal, chemical, physical] Beneficial Controlling/Restricting Pesticide Use Beneficial Controlling/Restricting Herbicide Use Beneficial Reforestation Beneficial Stocking captive-reared wild-strain animals Beneficial Controlling/Removing Exotic Vertebrates Adverse Hybridization Existing Hybridization Adverse Salinity alteration Existing Salinity alteration Adverse Water Level Fluctuation Existing Water Level Fluctuation Adverse Groundwater drawdown Existing Groundwater drawdown Adverse Irrigating Existing Irrigating Adverse Reservoirs Existing Reservoirs Adverse Competition Existing Competition Adverse Exotic/Feral/Introducted Species Existing Exotic/Feral/Introducted Species Adverse Existing COMMENTS ON MANAGEMENT PRACTICES - The Pecos gambusia faces two major threats: 1) Loss of habitat and 2) the inability to interact successfully with nonnative (exogenous) fish species, especially Gambusia. The known occurrences of G. nobilis indicate that the species once was more widespread (01). The Pecos River mainstream has been influenced by man for more than 100 years, first through water withdrawals for irrigation and more recently through the construction of mainstream dams for irrigation and flood control. These water uses have severely depleted natural flows in the river along major sections and caused drastic increases in salinities in the remaining reaches. Although the mainstream Pecos River probably was never important as permanent habitat, the mainstream served as a dispersal route between tributary springs and streams. The more important lateral habitats initially were impacted by extensive ground water pumping of the aquifers surrounding the Pecos River in the mid-1900's. This caused cessation of flow and extirpation of G. nobilis from Comanche Springs (02) and undoubtedly from the vicinity of Ft. Sumner, NM, as well as its tributary North Spring River; and caused reduced flow with Management Practices - 1 (DRAFT) - Management Practices Species GAMBUSIA, PECOS Species Id ESIS252006 Date 14 MAR 96 loss of habitat in other areas. As a result of these habitat losses, the fish became isolated in permanent springs and is totally dependent upon spring flow for their survival (01). Concurrent with stream flow reduction, average water salinity has increased substantially (10). Disappearance of Pecos gambusia from Leon Springs preceeded drying of the spring and is associated with inundation by a small reservoir and the presence of introduced species (16). Declining spring flow and/or periodic drying of irrigation ditches may have played a substantial role in the decline of Endangered fishes in the Balmorhea area (17) as well as the release of introduced species (18). The native fishes, which have evolved in communities with low species diversity, are often unable to compete with introduced species. The effects of competition on G. nobilis are well known and indicate that they were disappearing in the Balmorhea area because of the expansion of G. geiseri, a nonnative poeciliid introduced into the springs in the early 1930's. Predation on G. nobilis could be a major limiting factor in areas where no submerged vegetation or sufficiently shallow areas provide cover from predators. Predation by the centrarchids Lepomis cyanellus and/or Micropterus salmoides may have eliminated the introduced population of G. nobilis from Lake St. Francis on the Bitter Lake National Wildlife Refuge and also may have contributed to the failure of a population introduced into Geyser Spring, NM. Also, virtual absence of G. nobilis from the head pool of Diamond-Y Spring may be attributable partly to the presence of L. cyanellus and M. salmoides. Pecos gambusia hybridizes with congenors at Balmorhea (03), Leon Creek (18), and at Bitter Lake (01). Abundance of Pecos gambusia is inverse to abundance of congenors such as G. affinis. As stream flows decline, environmental conditions become more favorable for G. affinis and less favorable fo G. nobilis. It seems as if G. nobilis cannot coexist with G. affinis in eurythermal or ponded environments (01). APPROVED PLAN: U.S. Fish and Wildlife Service. 1983. Pecos Gambusia Recovery Plan. U.S. Fish and Wildlife Service, Albuquerque, New Mexico. 41 pp. The prime objective of the Recovery Plan is to improve the status of the Pecos gambusia to the point that survival of populations from the four major areas of occurrence is secured. Maintenance and enhancement of existing populations and their habitats should include: 1) Monitoring populations (numbers, condition, and age structure). 2) Monitoring the condition of their habitat, which should also include evaluation of proposed activities such as pumping ground water, surface water diversions, roads, oil and gas field activities and phreatophyte management. This would include the controlling of pollutants such as herbicides or pesticides, oil and gas drilling and associated pollutants, and maintaining the water flow and water table. Water right acquisition or land easements may be necessary to Management Practices - 2 (DRAFT) - Management Practices Species GAMBUSIA, PECOS Species Id ESIS252006 Date 14 MAR 96 achieve these restraints. It is also important to: 1) Regulate the introduction of novel exotic fishes. 2) Preclude immigration of novel exotic fishes. 3) Remove exotic fishes where possible from Pecos gambusia habitat. 4) Evaluate, protect, and enhance deficient habitats through habitat rehabilitation. 5) Further study the ecology (i.e., fecunity, food habits, competition, hybridization potential, etc.) of this species. 6) Determine systematic relationships between the four major areas of occurrence. Efforts should be made to reestablish Pecos gambusia within four (of nine original) historic collection areas. Such a reintroduction should follow in this order: 1) Thorough surveys of potential reintroduction sites. 2) Remedial improvement of habitats as needed. 3) Introduction of pure stock. 4) Monitoring reintroduced populations. 5) Reintroduction of sympatric native fish after this species is reestablished. Captive populations of Pecos gambusia from each of the four major areas should be maintained at Dexter NFH for scientific research, transplanting for mosquito control in areas of suitable habitat, and as a reserve gene pool. Pecos gambusia should be provided the full protection mandated by Federal, New Mexico, and Texas laws. Finally, public awareness of the uniqueness of the Pecos gambusia should be increased through the preparation of information pamphlets, interpretive programs, and the use of broadcast and print media. Management Practices - 3
                                   (DRAFT) - References
                                 Species GAMBUSIA, PECOS
                                  Species Id ESIS252006
                                      Date 14 MAR 96



     

References

***** REFERENCES FOR ALL NARRATIVES EXCEPT N-OCCURRENCE ***** 01 U.S. Fish and Wildlife Service. 1983. Pecos Gambusia Recovery Plan. U.S. Fish and Wildlife Service, Albuquerque, NM. 41 pp. 02 Hubbs, C. and V.G. Springer. 1957. A revision of the Gambusia nobilis species group, with descriptions of three new species, and notes on their variation, ecology, and evolution. Tex. J. Sci. 9:297-327. 03 Echelle, A.A. and A.F. Echelle. 1980. Status of the Pecos gambusia, Gambusia nobilis. U.S. Fish and Wildlife Service, Albuquerque, NM. Endangered Species Rep. No. 10. 73 pp. 04 Baird, S.F. and C. Girard. 1853. Descriptions of new subspecies of fishes collected by Mr. J.H. Clark on the U.S. and Mexican Boundary Survey, under Lt. Col. Jas D. Graham. Proc. Acad. Nat. Sci., Phila. 6:387-390. 05 Girard, C. 1959. Ichtyology of the boundary. U.S. and Mexican Boundary Survey. 3:1-85. 06 Garman, S. 1895. The Cyprinodonts. Mem. Mus. Comp. Zool. 19:1-179. 07 Regan, C.T. 1913. A revision of the Cyprinodont fishes of the subfamily Poeciliinae. Proc. Zool. Soc., London:977-1018. 08 Alvarez, J. 1950. Claves para la determinacion de species en los peces de las aguas continentales Mexicanas. Secretaria de Marina, Mexico. 144 pp. 09 Hubbs, C.L. 1926. Studies of the fishes of the order Cyprinodontes. VI. Material for a revision of American genera and species. Univ. Mich. Mus. Zool. Misc. Publ. 16. 87 pp. 10 Robins, C.R. and others. 1980. A list of common and scientific names of fishes, 4th ed. Sp. Publ. Am. Fish. Soc. 12. 174 pp. 11 Rivas, L.R. 1963. Subgenera and species groups in the Poeciliid fish genus Gambusia Poey. Copeia 1963:331-347. 12 Peden, A.E. 1973. Variation in anal spot expression of gambusine females and its effect on male courtship. Copeia 1973:250-263. 13 Deacon, J.E., et al. 1979. Fishes of North America Endangered, Threatened, or of special concern. Fisheries 4(2):29-44. 14 Hubbs, C. and D.T. Mosier. In press. Fecundity of Gambusia gaigei, an Endangered species. Copeia. 15 Guillory, V. 1980. Gambusia nobilis Baird and Girard, Pecos gambusia. In: Atlas of North American freshwater fishes. D.S. Lee, et. al. 544 pp. 16 Hubbs, C. 1980. The solution to the Cyprinodon bovinus problem: Eradication of a pupfish genome. Proc. 10th Desert Fishes Council Pp. 9-18. 17 Davis, J.R. 1979. Die-offs of an Endangered pupfish, Cyprinodon elegans (Cyprinodontidae). The Southwest Nat. 24:534-536. 18 Hubbs, C. 1982. Occurrence of exotic fishes in Texas waters. Pearce-Sollards Series. TX Mem. Mus. 36:19 pp. 19 Rosen, D.E. and R.M. Bailey. 1963. The Poeciliid fishes (Cyprinodontiformes), their structure, zoogeography, and systematics. Bull. Am. Mus. Nat. Hist. 126:1-176. References - 1 (DRAFT) - References Species GAMBUSIA, PECOS Species Id ESIS252006 Date 14 MAR 96 20 Hubbs, C. 1978. Result of an eradication program on the ecological relationships of fishes in Leon Creek, Texas. Southwest Nat. 23:487-496. 21 Bouma, R. 1984. A contribution to the management of Gambusia nobilis at Bitter Lake National Wildlife Refuge. Rep. to USFWS, Albuquerque, NM. 88 pp. 22 Bednarz, J.C. 1979. Ecology and status of the Pecos gambusia, Gambusia nobilis (Poeciliidae), New Mexico. Southwest Nat. 24:311- 322. 23 Hubbs, C. 1971. Competition and isolation mechanisms in the Gambusia affinis x G. heterochir hybrid swarm. Bull. TX Mem. Mus. 19:47 pp. 24 Hubbs, C. 1984. Changes in fish abundance with time of day and among years at a station in Lake Texoma. 1983 Trans. TX Chap. Am. Fish. Soc:42-57. 25 Jordan, D.S. and B.W. Evermann. 1898. Fishes of North America. Bull. U.S. Nat. Mus. 47(1):1-1240. ***** REFERENCES FOR N-OCCURRENCE NARRATIVE ONLY ***** 01 U.S. Fish and Wildlife Service. 1983. Pecos Gambusia Recovery Plan. U.S. Fish and Wildlife Service, Albuquerque, NM. 41 pp. 02 Bouma, R. 1984. A contribution to the management of Gambusia nobilis at Bitter Lake National Wildlife Refuge. Rep. to USFWS, Albuquerque, NM. 88 pp. 03 Echelle, A.A. and A.F. Echelle. 1980. Status of the Pecos gambusia, Gambusia nobilis. U.S. Fish and Wildlife Service, Albuquerque, NM. Endangered Species Rep. No. 10. 73 pp. 04 Bednarz, J.C. 1979. Ecology and status of the Pecos gambusia, Gambusia nobilis (Poeciliidae), New Mexico. Southwest Nat. 24:311-322. 05 Hubbs, C. 1978. Result of an eradication program on the ecological relationships of fishes in Leon Creek, Texas. Southwest Nat. 23:487-496. 06 Hubbs, C. and V.G. Springer. 1957. A revision of the Gambusia nobilis species group, with descriptions of three new species, and notes on their variation, ecology, and evolution. Tex. J. Sci. 9:297-327. 07 Girard, C. 1959. Ichtyology of the boundary. U.S. and Mexican Boundary Survey. 3:1-85. 08 Hubbs, C. 1980. The solution to the Cyprinodon bovinus problem: Eradication of a pupfish genome. Proc. 10th Desert Fishes Council. Pp. 9-18. 09 Hubbs, C.L. 1926. Studies of the fishes of the order Cyprinodontes. VI. Material for a revision of American genera and species. Univ. Mich. Mus. Zool. Misc. Publ. 16:87 pp. 10 Hubbs, C. and A.A. Echelle. 1973. Endangered non-game fishes of the Upper Rio Grande Basin. In: Endangered vertebrates of the southwest. W.C. Huey. NM Game and Fish:147-167. References - 2