(DRAFT) - Taxonomy
                              Species GAMBUSIA, AMISTAD
                                Species Id ESIS252010
                                   Date 14 MAR 96



TAXONOMY

NAME - GAMBUSIA, AMISTAD OTHER COMMON NAMES - GAMBUSIA, AMISTAD; GAMBUSIA, GOODENOUGH; GAMBUSIA and GOODENOUGH SPRINGS ELEMENT CODE - CATEGORY - Fish PHYLUM AND SUBPHYLUM - CHORDATA, CLASS AND SUBCLASS - OSTEICHTHYES, ORDER AND SUBORDER - ATHERINIFORMES, FAMILY AND SUBFAMILY - POECILIIDAE, GENUS AND SUBGENUS - GAMBUSIA, SPECIES AND SSP - AMISTADENSIS, SCIENTIFIC NAME - GAMBUSIA AMISTADENSIS AUTHORITY - TAXONOMY REFERENCES - COMMENTS ON TAXONOMY - Amistad Gambusia Gambusia amistadensis Peden, 1973 KINGDOM: Animal GROUP: Fish PHYLUM: Chordata CLASS: Osteichthyes ORDER: Atheriniformes FAMILY: Poeciliidae The Amistad gambusia (Gambusia amistadensis) is a member of the Gambusia nobilis species group and appears closely related to G. senilis and especially to the G. gaigei species group (including G. gaigei, G. hurtadoi and G. alvarezi) (01,02,03,04,05). The species is characterized by its relatively slender body, terminal mouth with numerous teeth on each jaw and with males having longer serrae on ray 4p of the gonopodium. Preserved specimens have strong crosshatching and numerous darkly pigmented crescent-shaped spots on the scale margins. The mid-dorsal stripe is narrow and the lateral stripe is broad. A short, dusky subocular bar is present. Adult females have a permanent median dark anal spot (01). The holotype of this extinct species is in the University of Michigan Museum of Zoology (UMMZ 190407), the U.S. National Museum (USNM 205858), the University of Texas' Natural History Collections (TNHC 7247) and the National Museum of Canada (NMC 70-373) (01). A Taxonomy - 1 (DRAFT) - Taxonomy Species GAMBUSIA, AMISTAD Species Id ESIS252010 Date 14 MAR 96 photograph of a male and female of this species and a comparative diagnostic analysis appears in Peden's original description of the Amistad gambusia (01). Common name synonyms for the Amistad gambusia include the Goodenough gambusia and Goodenough Springs gambusia. Taxonomy - 2
                                  (DRAFT) - Status
                              Species GAMBUSIA, AMISTAD
                                Species Id ESIS252010
                                   Date 14 MAR 96



STATUS

Coded Status Delisted Status - 1 (DRAFT) - Status Species GAMBUSIA, AMISTAD Species Id ESIS252010 Date 14 MAR 96 COMMENTS ON STATUS - U.S. STATUSES AND LAWS: The Amistad gambusia (Gambusia amistadensis) was designated an Endangered species pursuant to the Endangered Species Act of 1973 (50 CFR 17.11; P.L. 93-205, 87 Stat. 884; 16 U.S.C. 1531-1540), as amended. The species has been delisted due to extinction. RESPONSIBLE FEDERAL AGENCIES: USFWS -Responsible for the management/recovery, listing, and law enforcement/protection of this species. In addition the USFWS is responsible for delisting this particular species. All Federal agencies have responsibility to ensure that any action authorized, funded, or carried out by that agency is not likely to jeopardize the continued existence of the species or result in the destruction or adverse modification of Critical Habitat (50 CFR 402), and to utilize their authorities to carry out programs for the conservation of the species. STATE STATUSES AND LAWS: STATE: Texas DESIGNATED STATUS: Endangered ADMINISTRATIVE AGENCY: Texas Parks and Wildlife Department STATE STATUTE: 31 Texas Administrative Code Sec. 57.133 (127.30. 003) July 1977 INTERNATIONAL STATUSES, TREATIES, AND AGREEMENTS: The Amistad gambusia was listed as endangered in the IUCN Red Data Book, Vol. 4, 1977, but its status was changed to extinct in the 1986 IUCN Red List of Threatened Animals. ECONOMIC STATUSES: None. 78/08/15:43 FR 36117/36120 - Proposed listing as Endangered 80/04/30:45 FR 28721/28722 - Listing as Endangered 85/07/22:50 FR 29901/29909 - Five year review 87/03/11:52 FR 07462/07463 - Proposed rule - Delist (Extinct) 87/07/07:52 FR 25522/ - Notice of 5-year review completion 87/12/04:52 FR 46083/46085 - Final rule - Delisted (Extinct) Status - 2
     

HABITAT ASSOCIATIONS

HABITAT - AQUATIC INLAND AQUATIC COMMENTS ON HABITAT ASSOCIATIONS - Gambusia amistadensis was restricted to the rapidly flowing warm spring waters of Goodenough Springs and to the approximately 1.3 km outflow creek which emptied into the Rio Grande (01). Goodenough Springs, formerly the third largest springs of Texas (06), flowed over limestone gravel and sand substrates along this 1.3 km course to the Rio Grande. Waters originated in the relatively large Edwards-Trinity aquifer (08) and maintained flow rates of approximately 2000 to 4000 cubic liters per second (06). The type locality and habitat for the Amistad gambusia is now under approximately 30 meters of water behind Amistad Reservoir and spring flows are greatly reduced (01,06). This species is now considered extinct (07). Habitat Associations - 1
                                (DRAFT) - Food Habits
                              Species GAMBUSIA, AMISTAD
                                Species Id ESIS252010
                                   Date 14 MAR 96



FOOD HABITS

TROPHIC LEVEL - OMNIVORE LIFESTAGE FOOD FOOD PART General Detritus General Algae General Arthropods Food Habits - 1
                         (DRAFT) - Environment Associations
                              Species GAMBUSIA, AMISTAD
                                Species Id ESIS252010
                                   Date 14 MAR 96



ENVIRONMENTAL ASSOCIATIONS

Environment Associations - 1
                               (DRAFT) - Life History
                              Species GAMBUSIA, AMISTAD
                                Species Id ESIS252010
                                   Date 14 MAR 96



LIFE HISTORY

FOOD HABITS: Little is known concerning the food habits of the Amistad gambusia, however, the gut contents of 10 paratypes examined by Peden (01) contained mostly unidentified items, some insect fragments and traces of filamentous algae. HOME RANGE/TERRITORY: Territoriality and home ranges for the Amistad gambusia are unknown. Presumably when the species was extant, it behaved similarly to other species of Gambusia where males maintain breeding territories (09,10). PERIODICITY: No information is available on periodicity of the Amistad gambusia, however, most members of the genus are diurnal (11). MIGRATION PATTERNS: No information exists concerning migration, but, migration is unlikely. COVER/SHELTER REQUIREMENTS: Prior to its extinction, the Amistad gambusia inhabited shallow water environments at Goodenough Springs and its outflow which contained sparse stands of submerged aquatic vegetation (01). REPRODUCTIVE SITE REQUIREMENTS: Observations in aquaria of pregnant female Gambusia amistadensis indicated that these individuals gave birth to their young in vegetated areas and that presumably females sought similar areas in the wild to give birth to their young (01,09). REPRODUCTIVE CHARACTERISTICS: The reproductive behavior of the Amistad gambusia has been reported by Peden (09,10) who found that this species has similar breeding characteristics to other members of the Gambusia nobilis complex. Male courtship appears similar to other poeciliids (10). Of 10 female paratypes examined by Peden (01), the mean size was 29.8 mm standard length (SL) (range =25.9 to 34.6 mm SL) and 7 contained 5 to 11 (mean 8.9) embryos in each ovary while the other 3 females contained 1 to 7 eggs. PARENTAL CARE: Gambusia amistadensis is a member of the family Poeciliidae and all females bear their young alive. Following partuition, no further parental care is provided by the female (09,10). POPULATION BIOLOGY: All known populations of Gambusia amistadensis are extinct due to the inundation of their only known natural environment and the extirpation of this species in the two artificial cultures by mosquitofish (Gambusia affinis) (07). Captive populations were Life History - 1 (DRAFT) - Life History Species GAMBUSIA, AMISTAD Species Id ESIS252010 Date 14 MAR 96 maintained until the late-1970's at the Brackenridge Field Laboratory of the University of Texas at Austin and at the U.S. Fish and Wildlife Service's hatchery facility at Dexter, NM (Dexter NFH). Sometime during this period, both cultures became contaminated with mosquitofish (G. affinis) and all Amistad gambusia were extirpated (07). SPECIES INTERRELATIONSHIPS: Little informataion is known concerning other species' relationships with Gambusia amistadensis. Other fishes co-occurring with the Amistad gambusia prior to the inundation of its habitat included: Astyanax mexicanus (Mexican tetra), Hybopsis aestivalis (speckled dace), Notropis lutrensis (red shinner), N. braytoni (Tamaulipan shiner), N. jemezanus (Rio Grande shiner), N. proserpinus (proserpine shiner), N. venustus (blacktail shiner), Cycleptus elongatus (blue sucker), Icatalurus punctatus (channel catfish), I. melas (black bullhead), Pylodictus olivaris (flathead catfish), Gambusia affinis (mosquitofish), Micropterus salmoides (largemouth bass), Lepomis cyanellus (green sunfish), and Cichlasoma cyanoguttatum (Rio Grande cichlid) (01). OTHER LIFE HISTORY DESCRIPTORS: The Amistad gambusia (Gambusia amistadensis) is now extinct (07). In reporting the circumstances in the extinction of this species, Hubbs and Jensen cite potential hazards of attempting to maintain only culture populations of Endangered and Threatened species (07). Life History - 2
                           (DRAFT) - Management Practices
                              Species GAMBUSIA, AMISTAD
                                Species Id ESIS252010
                                   Date 14 MAR 96



MANAGEMENT PRACTICES

RESULT MANAGEMENT PRACTICE Adverse Hybridization Existing Hybridization Adverse Low Gene Pool Existing Low Gene Pool Adverse Reservoirs Existing Reservoirs COMMENTS ON MANAGEMENT PRACTICES - The Amistad gambusia's only known habitat at Goodenough Springs and its outflow was inundated by waters from the filling of Amistad Reservoir in 1968 (01,06). Captive populations were maintained until the late-1970's at the Brackenridge Field Laboratory of the University of Texas at Austin and at the U.S. Fish and Wildlife Service's hatchery facility at Dexter, NM (Dexter NFH). Sometime during this period, both cultures became contaminated with mosquitofish (G. affinis) and all Amistad gambusia were extirpated (07). In reporting the circumstances in the extinction of this species, Hubbs and Jensen cite potential hazards of attempting to maintain only culture populations of Endangered and Threatened species (07). UNAPPROVED PLAN: The U.S. Fish and Wildlife Service considers this species extinct. This species is considered extinct by the U.S. Fish and Wildlife Service and therefore no Recovery objectives have been identified. Management Practices - 1
                                   (DRAFT) - References
                                Species GAMBUSIA, AMISTAD
                                  Species Id ESIS252010
                                      Date 14 MAR 96



     

References

***** REFERENCES FOR ALL NARRATIVES EXCEPT N-OCCURRENCE ***** 01 Peden, A.E. 1973. Virtual extinction of Gambusia amistadensis n. sp., a poeciliid fish from Texas. Copeia 1973:210-221. 02 Hubbs, C. and V.G. Springer. 1957. A revision of the Gambusia nobilis species group, with descriptions of three new species and notes on their variation, ecology, and evolution. Texas J. Sci. 9:279-327. 03 Minckley, W.L. 1963. A new poeciliid fish (genus Gambusia) from the Rio Grande drainage of Coahuila, Mexico. Southwest. Nat. 8:145-161. 04 Rosen, D.E. and R.M. Bailey. 1963. The poeciliid fishes (Cyprinodontiformes), their structure, zoogeography, and systematics. Bull. Amer. Mus. Nat. Hist. 126:1-176. 05 Peden, A.E. 1972. Differences in the external gentalia of female gambussin fishes. Southwest. Nat. 17:265-272. 06 Brune, G. 1981. Springs of Texas. Vol. 1. Branch-Smith, Inc., Fort Worth, Texas. 07 Hubbs, C. and B.L. Jenson. 1984. Extinction of Gambusia amistadensis, an endangered fish. Copeia 1984:529-530. 08 Peckham, R.C. 1963. Summary of the ground-water resources in the Rio Grande Basin. Texas Water Comm. Circ. 63-05:1-16. 09 Peden, A.E. 1970. Courtship behavior of Gambusia (Poeciliidae) with emphasis on isolating mechanisms. Unpubl. Ph.D. Diss., Univ. of Texas, Austin, TX. 274 pp. 10 Peden, A.E. 1973. Variation in anal spot expression of gambussin females and its effect on male courtship. Copeia 1973:250-263. 11 Hubbs, C. 1971. Competition and isolation mechanisms in the Gambusia affinis x G. heterochir hybrid swarm. Bull. Tex. Mem. Mus. 19. 46 pp. ***** REFERENCES FOR N-OCCURRENCE NARRATIVE ONLY ***** 01 Peden, A.E. 1973. Virtual extinction of Gambusia amistadensis n. sp., a poeciliid fish from Texas. Copeia 1973:210-221. 02 Brune, G. 1981. Springs of Texas. Vol. 1. Branch-Smith, Inc., Fort Worth, Texas. 03 Hubbs, C. and B.L. Jenson. 1984. Extinction of Gambusia amistadensis, an Endangered fish. Copeia 1984:529-530. 04 U.S. Fish and Wildlife Service. 1987. Endangered and threatened wildlife and plants; Removal of Gambusia amistadensis, the Amistad gambusia from the list of endangered and threatened wildlife. Federal Register 52:46083-46085 References - 1