(DRAFT) - Taxonomy
                               Species CAVEFISH, OZARK
                                Species Id ESIS254003
                                   Date 13 MAR 96



TAXONOMY

NAME - CAVEFISH, OZARK OTHER COMMON NAMES - CAVEFISH and OZARK ELEMENT CODE - CATEGORY - Fish PHYLUM AND SUBPHYLUM - CHORDATA, CLASS AND SUBCLASS - OSTEICHTHYES, ORDER AND SUBORDER - PERCOPSIFORMES, FAMILY AND SUBFAMILY - AMBLYOPSIDAE, GENUS AND SUBGENUS - AMBLYOPSIS, SPECIES AND SSP - ROSAE, SCIENTIFIC NAME - AMBLYOPSIS ROSAE AUTHORITY - TAXONOMY REFERENCES - COMMENTS ON TAXONOMY - Ozark Cavefish Amblyopsis rosae (Eigenmann, 1898) KINGDOM: Animal GROUP: Fish PHYLUM: Chordata CLASS: Osteichthyes ORDER: Percopsiformes FAMILY: Amblyopsidae The Ozark cavefish, Amblyopsis rosae (Eigenmann), is a member of the family Amblyopsidae, a small group of North American fishes which show various adaptions to life in the subterranean environment. The family as revised by Woods and Inger (02) contains three genera and five species, including the epigean species Chologaster cornuta, the troglophile Chologaster agassize, and the troglobites Typhlichthys subterraneus, Amblyopsis spelaea, and Amblyopsis rosae. A fourth genus, Speoplatyrhinus poulsoni, was described by Cooper and Kuehne (03). The family's distribution lies north of the Mississippi Embayment and south of the last glaciation, in the eastern half of the United States (02). Amblyopsis rosae is a small (5-7 cm total length), white fish with a broad flattened head, small scales, and no eyes (04). Postcleithrum are absent and sensory papillae on anal fin are in four to six rows (02). The anus migrates forward to the throat in adults Taxonomy - 1 (DRAFT) - Taxonomy Species CAVEFISH, OZARK Species Id ESIS254003 Date 13 MAR 96 (04). A. rosae can be differentiated from congener Amblyopsis spelaea by absence of pelvic fins in A. rosae. Amblyopsis rosae was first collected from Sarcoxie Cave (also referred to as Day's Cave and Downer's Cave), but was reported as Typhlichthys subterraneus by Garman (05). Eigemann (01) characterized the specimen as a new genus and named it Troglichthys rosae. Woods and Inger (02) later placed Troglichthys rosae in the genus Amblyopsis. The confusion between A. rosae and Typhlichthys still exists with positive identification possible only by x-ray analysis of the vertebral column or dissection of the postcleithrum in Typhlichthys. Actually, the two genera are believed to be of separate lineages (02). The published report of Typhlichthys locations in the Springfield Plateau (06) has recently been found to be A. rosae (07). A. rosae is the only Amblyopsid found in the Springfield Plateau (08). Willis and Brown (09) speculated that each cave may represent a separate evolutionary event. How this would effect species integrity is uncertain. Taxonomy - 2
                                  (DRAFT) - Status
                               Species CAVEFISH, OZARK
                                Species Id ESIS254003
                                   Date 13 MAR 96



STATUS

Coded Status Arkansas; State Recognized T: Federal Threatened Ornamental COMMENTS ON STATUS - U.S. STATUSES AND LAWS: The Ozark cavefish (Amblyopsis rosae) has been designated an Threatened species pursuant to the Endangered Species Act of 1973 (50 CFR 17.11; P.L. 93-205, 87 Stat. 884; 16 U.S.C. 1531-1540), as amended. The species has this status wherever found including the States of AR, MO, and OK. This species is protected by the Lacey Act (P.L. 97-79, as amended; 16 U.S.C. 3371 et seq.) which makes it unlawful to import, export, transport, sell, receive, acquire, or purchase any wild animal (alive or dead including parts, products, eggs, or offspring): (1) in interstate or foreign commerce if taken, possessed, transported or sold in violation of any State law or regulation; or (2) if taken or possessed in violation of any U.S. law, treaty, or regulation or in violation of Indian tribal law. It is also unlawful to possess any wild animal (alive or dead including parts, products, eggs, and offspring) within the U.S. territorial or special maritime jurisdiction (as defined in 18 U.S.C. 7) that is taken, possessed, transported, or sold in violation of any State law or regulation, foreign law, or Indian tribal law. RESPONSIBLE FEDERAL AGENCIES: USFWS -Responsible for the management/recovery, listing, and law enforcement/protection of this species. USFS -Responsible for the law enforcement/protection of this species with applicable State and Federal laws on public lands under their control. Also responsible for management/recovery on Forest Service lands. The Forest Service is responsible for integrating management, protection, and conservation of Federally listed species into the Forest Planning process (36 CFR 219.19 and 219.20). Management practices that would cause detrimental changes in water temperature or composition, water course blockage, or sediment deposits within 100 feet Status - 1 (DRAFT) - Status Species CAVEFISH, OZARK Species Id ESIS254003 Date 13 MAR 96 of the edges of perennial streams, lakes or other bodies of water are prohibited (36 CFR 219.27(e)). All Federal agencies have responsibility to ensure that any action authorized, funded, or carried out by that agency is not likely to jeopardize the continued existence of the species or result in the destruction or adverse modification of Critical Habitat (50 CFR 402), and to utilize their authorities to carry out programs for the conservation of the species. STATE STATUSES AND LAWS: STATE: Arkansas DESIGNATED STATUS: Recognized Threatened ADMINISTRATIVE AGENCY: Game and Fish Commission STATE STATUTES: Amendment 35 to State Constitution STATE: Missouri DESIGNATED STATUS: Endangered ADMINISTRATIVE AGENCY: Department of Wildlife Conservation STATE STATUTES: Revised, Statutes of Missouri 252-240, Regulation 3 CFR 10-4.111 STATE: Oklahoma DESIGNATED STATUS: Recognized Threatened ADMINISTRATIVE AGENCY: Department of Wildlife Conservation STATE STATUTES: Sec. 2-135 and Title 29 OK Game and Fish Laws (Introduced Legislation). Sec 5-412. INTERNATIONAL STATUSES: The Ozark cavefish is listed as Vulnerable in the IUCN Red Data Book, Vol. 4, 1977. ECONOMIC STATUSES: Commercially valuable to aquarists. 83/02/15:48 FR 06752/ - Finding on petition 84/01/31:49 FR 03889/03891 - Proposed rule 84/11/01:49 FR 43965/43969 - Final rule, listed as Threatened Status - 2
     

HABITAT ASSOCIATIONS

HABITAT - AQUATIC TERRESTRIAL INLAND AQUATIC LAND USE - Residential Commercial and Services Industrial Transportation, communications, and Util Industrial and Commercial Complexes Mixed Urban or Built-up Land Cropland and Pasture Orchards, Groves, Vineyards, Nurseries, Confined Feeding Operations Deciduous Forest Land Streams and Canals Reservoirs NATIONAL WETLAND INVENTORY CODES NWI NWICLS NWIMOD NWISPEC Riverine, upper perennial RB2 Riverine, upper perennial RB1 COMMENTS ON HABITAT ASSOCIATIONS - The caves inhabited by Amblyopsis are unique systems. Caves are well known for constant temperature, and complete darkness. Lacking light, they are completely dependent upon allochthonous energy sources (11). This results in low diversity of food and organisms. The most important sources of this food input in cavefish caves are bats and flooding (08). Both of these inputs are dependent upon openings. Because the Myotis grisescens (gray bats) in this area have been unable to successfully use gated caves, cave gating is not an alternative in cavefish management. Windblown and sinkhole introduction of organic matter is also great in some instances. The maintenence of these cave openings is essential to the survival of these fish. Cave openings can occur anywhere. Typically they occur in rock bluffs at the head of a spring. However, many cavefish caves have entrances in open fields or forests. Land use in A. rosae habitat is varied. Two of the known sites are used as water supplies for trout farms. One is directly under a dairy farm, and another under a flower farm. One site underlies a relatively new housing development while two are in the heart of long established small towns. Both of these towns once used the caves as municipal water supplies, while another site was once used as the water supply for a U.S. Army Camp. It is also believed that the water level of one site may be effected by water levels of a local U.S. Corps of Engineers reservoir, Grand Lake O' the Cherokees. Integral to protecting the cave environment is protecting the caves water supply. This includes the surface drainage basins and aquifers which feed the caves. Although A. rosae is rarely seen in surface streams, these streams often lose water to aquifers or "sink" entirely into caves. Numerous examples of sinking streams are found in the cavefish range. The karst nature of the Springfield Plateau results in rapid transfer of surface runoff to the numerous caves with relatively little filtration. These characteristics also facilitate Habitat Associations - 1 the movement of pollutants to the cave environment. Therefore, management of this environment should not only include the cave itself, but surrounding surface practices as well. Specific environmental parameter values of caves which are inhabited by A. rosae are given in Willis (08), and Poulson (12). In summary of Willis's findings, little variation is seen between caves in pH (6.0-6.7), turbidity (2.6-15.5 NTU), and temperature (12-16 degrees C). The variation in dissolved oxygen (3.4-11.8 ppm), conductivity (129-472 umhos), total organic carbon (2.78-57.60 ppm), alkalinity (30-230 ppm), ammonia (0.01-3.8 ppm), nitrates and nitrites (1.2-5.8 ppm), and T-phosphorous (0.01-0.16 ppm) indicates fairly wide ranges of tolerance for these components. Amblyopsis rosae habitat must include still water due to the species' negative rheotaxis (i.e., the species avoids currents) (12, 13). In almost all cases the caves which contain A. rosae populations have unsurveyable areas such as lower flooded passages or siphons (areas where the ceiling comes down to the water level). The importance of these areas as refugia are not known; however, they may be of great importance in caves which receive much traffic. Habitat Associations - 2
                                (DRAFT) - Food Habits
                               Species CAVEFISH, OZARK
                                Species Id ESIS254003
                                   Date 13 MAR 96



FOOD HABITS

TROPHIC LEVEL - CARNIVORE LIFESTAGE FOOD FOOD PART General Detritus General Arthropods General Fish General Amphibia General Zooplankton General Crustaceans Food Habits - 1
                         (DRAFT) - Environment Associations
                               Species CAVEFISH, OZARK
                                Species Id ESIS254003
                                   Date 13 MAR 96



ENVIRONMENTAL ASSOCIATIONS

G = General A = Adult LIM = Limiting RA = Resting Adult J = Juvenile FA = Feeding Adult RJ = Resting Juvenile BA = Breeding Adult FJ = Feeding Juvenile P = Pupae L = Larvae E = Egg RL = Resting Larvae FL = Feeding Larvae
LIFESTAGE ENVIRONMENTAL ASSOCIATIONS G Aquatic Features: Springs [flowing] G Terrestrial Features: Caves, wet G Terrestrial Features: Caves, dry G Terrestrial Features: Depressions G Terrestrial Features: Cliffs/ledges G Terrestrial Features: Rock outcrops Environment Associations - 1
                               (DRAFT) - Life History
                               Species CAVEFISH, OZARK
                                Species Id ESIS254003
                                   Date 13 MAR 96



LIFE HISTORY

FOOD HABITS: From the limited food habit studies done on Amblyopsis rosae, it can be assumed they will eat anything that will fit into their mouths and is useful. Poulson (12) found copepods, cladocerans, ostracods, isopods, amphipods, small crayfish, coleopteran larvae, plecopteran nymphs, crickets, larval salamanders, young of their own species, and bat guano. This virtually comprises all forms of organic material in the caves in which the fish were located. Willis (08) and Poulson (12) give relative food availability data for the various A. rosae habitats. There is nothing known about food requirements for the species. However, Poulson (12) reports that because of A. rosae's low metabolic rate, it is able to go without food for long periods (401 days before activity ceased). Poulson also found that based on stomach contents and fat deposits, A. rosae fed less in October and September. This is also the time of annulus formation. HOME RANGE/TERRITORY: No territoriality is known in Amblyopsis rosae. Individuals may be found in a given area over several months. This is certainly a form of energy conservation (14). PERIODICITY: There is no known periodicity in this species. Observations have revealed similar numbers at all times of the day and year (14). MIGRATION PATTERNS: There is no known migration activity for Amblyopsis rosae. COVER/SHELTER REQUIREMENTS: Other than the obvious needs of a cave and water, little is known about what cover is necessary for Amblyopsis rosae. There is a strong indication of a negative rheotaxis (12,14). Therefore, there is a need for still water. The depth at which fish have been observed ranges from 4 m to 4 cm. Poulson (12) reports a preference for chert substrate; however, they have often been seen on clay substrate (14). REPRODUCTIVE SITE REQUIREMENTS: No requirements for breeding are known for Amblyopsis rosae. Perhaps the greatest obstacle is finding mates. REPRODUCTIVE CHARACTERISTICS: No breeding behavior has been documented for Amblyopsis rosae, however, Poulson (13) has published a life history account for the species. Poulson found (12,13) free swimming fry at 5-6 months of age, first scales at 10-12 months (length=12-14 mm), first annulus formation at 19-21 months (length=16-24 mm), first reproduction at 36-48 months (length=36-42 mm), and oldest individuals at 45-57 months (length=44-52 mm). Poulson (15) now questions his original length of life estimate and may be off by a factor of 3-4. Fertilization probably occurs from February to April; however, it is believed that less than one fifth of the females may breed in Life History - 1 (DRAFT) - Life History Species CAVEFISH, OZARK Species Id ESIS254003 Date 13 MAR 96 any given year. Poulson (13) believes that A. rosae may be a branchial brooder like its congener, A. spelaea. In A. spelaea, young are carried in the gill cavity until they lose their yolk sac, a total of 4-5 months. The mean number of ova per female is 23 (12). PARENTAL CARE: The only evidence of parental care is in that of Amblyopsis spelaea, the northern cavefish. A. Spelaea is a branchial brooder, carrying the eggs and fry in its gill chamber from fertilization to loss of yolk sac. This is a period of 4-5 months. Poulson (12,13) has proposed this behavior for Amblyopsis rosae also. POPULATION BIOLOGY: Very little is known about how populations of Amblyopsis rosae may change through time. The largest known population has been surveyed on two separate occasions at 97 and 100 individuals (08). All other locations have been counted at less than twelve individuals with two sites having only one fish each (08). Because all of these caves have areas that are unsurveyable, there is the possibility of more fish. Poulson (12) constructed age distribution tables; however, the usefulness of these is questionable since they may be off by a factor of 3-4 (15). Poulson's old data corrected by a factor of 3-4 indicates a maximum life span of 12-16 years. With the low reproductive rate (r=0.002) (12), low numbers per population and few populations, the species has a very low recovery potential. SPECIES INTERRELATIONSHIPS: Amblyopsis rosae is highly dependent upon the gray bat, Myotis grisescens, for importation of guano. A. rosae may eat bat guano directly; however, it is probably more important as the base of a longer food chain. Poulson (12,13) found A. rosae's diet contained, by volume, 80% copepods. This indicates a dependence on these microcrustaceans. Willis (08) found that of the 13 caves which contained cavefish, 9 also contained troglobitic crayfish. However, the one cave that contained two thirds of the fish did not have crayfish. The nature of cave crayfish interactions are not known other than cavefish occasionally eat cave crayfish young. Cave crayfish are also capable of catching and eating adult A. rosae (14). OTHER LIFE HISTORY DESCRIPTORS: None. Life History - 2
                           (DRAFT) - Management Practices
                               Species CAVEFISH, OZARK
                                Species Id ESIS254003
                                   Date 13 MAR 96



MANAGEMENT PRACTICES

RESULT MANAGEMENT PRACTICE Beneficial Restricting/regulating human disturbance of populations Beneficial Maintaining/Controlling Water Flow Beneficial Controlling water levels Beneficial Maintaining undisturbed/undeveloped areas Beneficial Land Acquisition Beneficial Restricting Poaching Adverse Harassment/Vandalism/Indiscriminate Killing Existing Harassment/Vandalism/Indiscriminate Killing Adverse Collecting Existing Collecting Adverse Spelunking Existing Spelunking Adverse Food Supply Reduction Existing Food Supply Reduction Adverse Inherent Reproductive Characteristics Existing Inherent Reproductive Characteristics Adverse Low Gene Pool Existing Low Gene Pool Adverse Groundwater drawdown Existing Groundwater drawdown Adverse Applying pesticides Existing Applying pesticides Adverse Environmental Contamination/Pollution Existing Environmental Contamination/Pollution Adverse Competition Existing Competition COMMENTS ON MANAGEMENT PRACTICES - Many of the reasons for the status of Amblyopsis rosae are linked to inherent biological traits of the species, (i.e. low reproductive rate, small population size, high degree of specialization) (08). Those traits reduce species stability. Perhaps, more important is the attention this species has attracted from amateur and scientific collectors. Many scientific collections of every fish in a particular cave have been made (10). Because there is no primary production in caves, the energy and nutrient supplies of the cave ecosystem are based upon what is brought in from the outside. The closing of cave entrances and loss of bat colonies greatly reduce this food input. Willis and Brown (09) reported 86% of the cavefish seen were in caves which had colonies of the endangered species, Myotis grisescens (gray bat), present. A. rosae is dependent upon Myotis grisescens or some other source for energy/nutrient input. Eleven other cavefish caves had some other source of organic enrichment. Although it has not been confirmed, it is thought (08,09) that ground water pollution may be responsible for the erradication of some populations near Springfield, Missouri. Another problem in the A. rosae range is ground water draw down. How this has effected the Management Practices - 1 (DRAFT) - Management Practices Species CAVEFISH, OZARK Species Id ESIS254003 Date 13 MAR 96 cavefish is unknown; however, many caves in the area which once contained water are now dry. The effect of disturbance (i.e. walking through the cave) is also unconfirmed. It is known that many caves which once contained cavefish, but now contain few or none, are often highly used by cave explorers; and that caves which still have many cavefish are protected or relatively unused. In addition to the above, potential threats include surface chemical spills, high pesticide use, and competition with newly invading surface species. APPROVED PLAN: U.S. Fish and Wildlife Service. 1986. A Recovery Plan for the Ozark Cavefish (Amblyopsis rosae). U.S. Fish and Wildlife Service, Atlanta, GA. 42 pp. The Recovery Plan for the Ozark cavefish specifically recommends: 1) Study of local and regional hydrological patterns to determine the recharge area and the extent of continuous habitat. 2) Provide protection and management for the recovery of caves. Protection of habitat may include conservation agreements or land acquisition; limiting human access by installing gates to decrease disturbance by cavers; and, developing and implementing protection strategies to prohibit collecting. Management of the habitat may include the introduction of bats into the uncolonized recovery caves, and an educational program for spelunkers promoting awareness of the harm caving can inflict upon cavefish. 3) Develop and implement a monitoring program to monitor water quality. Implementation may involve maintaining the water table and water flow. It may also be necessary to monitor development as it may effect water quality. The Nature Conservancy and Arkansas Natural Heritage Commission have purchased the cave with the best Ozark cavefish population. The Missouri Department of Wildlife Conservation owns a cave with a small population of Ozark cavefish. The Fish and Wildlife Service is negotiating the purchase of the cave with a good population of Ozark cavefish. The Arkansas Game and Fish Commission owns a cave in eastern Benton County, AR, that was discovered in 1987. Management Practices - 2
                                   (DRAFT) - References
                                 Species CAVEFISH, OZARK
                                  Species Id ESIS254003
                                      Date 13 MAR 96



     

References

***** REFERENCES FOR ALL NARRATIVES EXCEPT N-OCCURRENCE ***** 01 Eigenmann, C. H. 1898. A new blind fish. Proc. Ind. Acad. Sci. 1897:231. 02 Woods, L. P. and R. E. Inger. 1957. The cave, spring, and swamp fishes of the family Amblyopsidae for the central and eastern United States. Am. Midl. Nat. 58:232-256. 03 Cooper, L. E. and R. A. Kuehne. 1974. Speoplatyrhinus poulsoni, a new genus and species of subterranean fish from Alabama. Copeia 1972:486-493. 04 Pflieger, W. L. 1975. The Fishes of Missouri. Missouri Dept. Conserv. 343 pp. 05 Garman, S. 1889. Cave animals from southwestern Missouri. Bull. Mus. Comp. Zool. 17:232. 06 Hall, G. F. 1956. Additions to the fish fauna of Oklahoma with a summary of introduced species. Southwest. Nat. 1(1):16-26. 07 Mayden, R. L. and F. B. Cross. 1983. Reevaluation of Oklahoma records of the southern cavefish Typhlichthys subterraneus Girard (Amblyopsidae). Southwest. Nat. 28(4):471-473. 08 Willis, L. D. 1984. Distribution and habitat requirements of the Ozark cavefish, Amblyopsis rosae. M.S. Thesis. Univ. Ark. 25 pp. 09 Willis, L. D. and A. V. Brown. In press. Distribution and habitat requirements of the Ozark cavefish, Amblyopsis rosae. Am. Midl. Nat. 10 Museum Records (Tulane #10719, 16723, 11602, and 16561). 11 Barr, T. C. 1968. Cave ecology and the evolution of troglobites. Evolut. Biol. 2:35-102. 12 Poulson, T. L. 1961. Cave adaptation in amblyopsid fishes. Ph.D. Dissertation. Univ. Mich. 185 pp. Univ. microfilms. Ann Arbor, Michigan. 13 Poulson, T. L. 1963. Cave adaption in amblyopsid fishes. Am. Midl. Nat. 70:257-290. 14 Willis, L. D. Unpublished data. Dept. Biology, Va. Polytech. Inst. St. Univ., Blacksburg, VA 24060. 15 Poulson, T. L. 1982. Personal communication. ***** REFERENCES FOR N-OCCURRENCE NARRATIVE ONLY ***** 01 Willis, L. D. 1984. Distribution and habitat requirements of the Ozark cavefish, Amblyopsis rosae. M.S. Thesis. Univ. Arkansas. 25 pp. 02 Willis, L. D. and A. V. Brown. In Press. Distribution and habitat requirements of the Ozark cavefish. 03 Quinn, J. H. 1957. Plateau surface of the Ozarks. Ark. Acad. Sci. 11:36-43. 04 Unofficial sightings. Reported by Tom Aley, Ozark Underground Laboratories, Protem, MO. Presented to the Missouri Department of Conservation. References - 1