(DRAFT) - Taxonomy
                                Species PIGTOE, ROUGH
                                Species Id ESIS404019
                                   Date 13 MAR 96



TAXONOMY

NAME - PIGTOE, ROUGH OTHER COMMON NAMES - PIGTOE, ROUGH; MUSSEL, PEARLY, PIGTOE, ROUGH; PIGTOE and WHITE ELEMENT CODE - CATEGORY - Aquatic Molluscs PHYLUM AND SUBPHYLUM - MOLLUSCA, CLASS AND SUBCLASS - BIVALVIA, ORDER AND SUBORDER - UNIONOIDA, FAMILY AND SUBFAMILY - UNIONIDAE, GENUS AND SUBGENUS - PLEUROBEMA, SPECIES AND SSP - PLENUM, SCIENTIFIC NAME - PLEUROBEMA PLENUM AUTHORITY - TAXONOMY REFERENCES - COMMENTS ON TAXONOMY - Rough Pigtoe Pleurobema plenum (Lea, 1840) KINGDOM: Animal GROUP: Clam PHYLUM: Mollusca CLASS: Bivalvia ORDER: Unionoida FAMILY: Unionidae P. plenum is a medium-sized species occurring in medium to large rivers (01). Valves are subtriangular in outline, heavy and solid, with high, full beaks located centrally and turned anteriorly (01). The anterior end is sharply truncated, dorsal margin is slightly curved, ventral margin is rounded, and the posterior margin is almost straight (02). The posterior ridge is narrowly rounded and ends bluntly. A slight sulcus occurs in most older specimens (01). The median ridge is high, wide, and rounded, separated from the posterior ridge by a radial depression (02). The periostracum has irregular growth lines and is satin-like in appearance, yellow-brown to red-brown in color. Beak cavity is deep and compressed; muscle scars are small but deep (02). Nacre color varies from white to pink and is often iridescent posteriorly (03). No sexual dimorphism occurs in this species. P. plenum belongs to a P. cordatum complex containing 4 closely Taxonomy - 1 (DRAFT) - Taxonomy Species PIGTOE, ROUGH Species Id ESIS404019 Date 13 MAR 96 related species; cordatum, coccineum, rubrum, and plenum. These species have overlapping distributions and occur together in the same localities (04,05). Although the taxonomic status of P. plenum was clarified (06), differentiation of these forms is difficult. The status of species is based on the fact that identifiable specimens occur in the same mussel beds as other species of the cordatum group, with no apparent intergrades (02). Detailed descriptions and photographs of this species are presented in previous reports (01,02,07). Taxonomy - 2
                                  (DRAFT) - Status
                                Species PIGTOE, ROUGH
                                Species Id ESIS404019
                                   Date 13 MAR 96



STATUS

Coded Status Alabama; Federal Endangered Alabama; Unofficially Listed Arkansas; Federal Endangered Kentucky; Federal Endangered Kentucky; State Recognized Tennessee; Federal Endangered Tennessee; State Listed Virginia; Federal Endangered Virginia; State Recognized West Virginia; Federal Endangered E: Federal Endangered COMMENTS ON STATUS - U.S. STATUSES AND LAWS: The rough pigtoe (Pleurobema plenum) has been designated an Endangered species pursuant to the Endangered Species Act of 1973 (50 CFR 17.11; P.L. 93-205, 87 Stat. 884; 16 U.S.C. 1531-1540), as amended. This species historically occurred in AL, AR, IL, IN, IA, KS, KY, MO, OH, PA, TN, VA, and WV. However, it is listed in the Federal Register as historically occurring only in KY, TN, and VA. This species is protected by the Lacey Act (P.L. 97-79, as amended; 16 U.S.C. 3371 et seq.) which makes it unlawful to import, export, transport, sell, receive, acquire, or purchase any wild animal (alive or dead including parts, products, eggs, or offspring): (1) in interstate or foreign commerce if taken, possessed, transported or sold in violation of any State law or regulation; or (2) if taken or possessed in violation of any U.S. law, treaty, or regulation or in violation of Indian tribal law. It is also unlawful to possess any wild animal (alive or dead including parts, products, eggs, and offspring) within the U.S. territorial or special maritime jurisdiction (as defined in Status - 1 (DRAFT) - Status Species PIGTOE, ROUGH Species Id ESIS404019 Date 13 MAR 96 18 U.S.C. 7) that is taken, possessed, transported, or sold in violation of any State law or regulation, foreign law, or Indian tribal law. RESPONSIBLE FEDERAL AGENCIES: USFWS -Responsible for the management/recovery, listing, and law enforcement/protection of this species. DOD (COE) -Responsible for the law enforcement/protection of this species with applicable State and Federal laws on public land under their control. Also responsible for management/recovery on Department of Defense lands. NPS -Responsible for the law enforcement/protection of this species with applicable State and Federal laws on public lands under their control. Also responsible for conservation (Nat. Park System Organic Act - 16 U.S.C. 1, 2-3)/management/recovery on National Park Service lands. Taking, possessing, or disturbing of Federally listed species is prohibited on NPS lands (36 CFR 2.1, 2.2, and 2.3). All Federal agencies have responsibility to ensure that any action authorized, funded, or carried out by that agency is not likely to jeopardize the continued existence of the species or result in the destruction or adverse modification of Critical Habitat (50 CFR 402), and to utilize their authorities to carry out programs for the conservation of the species. STATE STATUSES AND LAWS: STATE: Alabama UNOFFICIAL LIST: Endangered, Bulletin No.2, Endangered and Threatened Plants and Animals of Alabama, Alabama Museum of Natural History. STATE: Arkansas, Illinois, and Tennessee DESIGNATED STATUS: Endangered ADMINISTRATIVE AGENCY: (AR) Game and Fish Commission; (IL) Department of Conservation; (TN) Wildlife Resources Agency. STATE STATUTES: (AR) Amendment #35 of Arkansas State Constitution 1945; (IL) Revised Statute 1983, Chapter 8, Section 331-340, P.A. 77-2186; (TN) Annotated Code, Chapter 9, Section 51-901 through 51-912, also Proclamations 75-15, 76-4 and amendments. STATE: Indiana, Iowa, Kansas, Kentucky, Missouri, Ohio, Pennsylvania, and Virginia DESIGNATED STATUS: Recognized Endangered ADMINISTRATIVE AGENCY: (IA) Conservation Commission; (IN) Department of Natural Resources; Status - 2 (DRAFT) - Status Species PIGTOE, ROUGH Species Id ESIS404019 Date 13 MAR 96 (KS) Fish and Game Commission; (KY) Department of Fish and Wildlife Resources; (MO) Department of Conservation; (OH) Department of Natural Resources; (PA) Game Commission; (VA) Commission of Game and Inland Fisheries. STATE STATUTES: (IA) Code of Iowa, Chapter 109A; (IN) Statutes Annotated, Chapt. 14-2-8.5-1; 310 IN Admin. Code 3-3-6; (KS) Statutes Annotated 32-502(d), (j), 504; (KY) Fish and Wildlife Codes, 150.183, Importing, transporting, or possessing endangered species of wildlife; (MO) Wildlife Code of Missouri, 3 CSR 10-4.111; (OH) Annotated Code 1501:31-23-01, Eff. July 11, 1980; (PA) 58 PA Code, Sec. 147.1, 147.21, Mar. 1983; (VA) State Code 29.230 to 29.237. STATE: West Virginia DESIGNATED STATUS: None. INTERNATIONAL STATUSES, TREATIES, AND AGREEMENTS: CITES I ECONOMIC STATUSES: There are no known commercial values for this species, however, its sedentary nature and filter-feeding habits may make it useful as a water quality indicator. It has been shown that mussels accumulate such pollutants as heavy metals and pesticides. 74/10/17:39 FR 37078/37079 - Notice of review 75/09/26:40 FR 44329/44333 - Proposed rule 76/06/14:41 FR 24062/24047 - Final rule 76/06/16:41 FR 24367/24378 - Proposal - CITES I 77/02/22:42 FR 10484/10485 - Final - CITES I 81/02/27:46 FR 14652/14658 - Five year review 87/07/07:52 FR 25523/25528 - Notice of review Status - 3
     

HABITAT ASSOCIATIONS

HABITAT - AQUATIC INLAND AQUATIC LAND USE - Streams and Canals NATIONAL WETLAND INVENTORY CODES NWI NWICLS NWIMOD NWISPEC Riverine, upper perennial UB1 Riverine, upper perennial SB1 COMMENTS ON HABITAT ASSOCIATIONS - P. plenum is a species found in medium to large rivers with sand and gravel substrate (01). It has been collected in muddy sand on the Green River and in sand on the Clinch River (04). The species does not occur in the impounded sections of rivers and is apparently quite sedentary in the substrate. Nothing is known about specific environmental requirements for adults or any other life stage. Habitat of the glochidia is initially within the gills of the female, then in the water column, and finally attached to a suitable fish host. Any alteration of these life stage-specific habitats during its life cycle would likely affect the long-term success of a population. Habitat requirements or associations for the juvenile stage are unknown. Habitat Associations - 1
                                (DRAFT) - Food Habits
                                Species PIGTOE, ROUGH
                                Species Id ESIS404019
                                   Date 13 MAR 96



FOOD HABITS

TROPHIC LEVEL - OMNIVORE LIFESTAGE FOOD FOOD PART General Detritus General Bacteria General Phytoplankton General Diatoms General Zooplankton General Fish Food Habits - 1
                         (DRAFT) - Environment Associations
                                Species PIGTOE, ROUGH
                                Species Id ESIS404019
                                   Date 13 MAR 96



ENVIRONMENTAL ASSOCIATIONS

G = General A = Adult LIM = Limiting RA = Resting Adult J = Juvenile FA = Feeding Adult RJ = Resting Juvenile BA = Breeding Adult FJ = Feeding Juvenile P = Pupae L = Larvae E = Egg RL = Resting Larvae FL = Feeding Larvae
LIFESTAGE ENVIRONMENTAL ASSOCIATIONS G Aquatic Features: Pool areas Environment Associations - 1
                               (DRAFT) - Life History
                                Species PIGTOE, ROUGH
                                Species Id ESIS404019
                                   Date 13 MAR 96



LIFE HISTORY

FOOD HABITS: There are no known interspecific differences in feeding among freshwater mussels (08). Glochidia are probably obligate parasites on the gills or fins of fish. Adults are filter-feeders and consume particulate matter suspended in the water column. Identifiable stomach contents almost invariably include mud, desmids, diatoms, other unicellular algae, protozoa, and zooplankters (14,15). No study of the food habits of P. plenum has been conducted; required/preferred foods are therefore unknown. HOME RANGE/TERRITORY: Non-territorial. The species is sedentary during its adult life and generally occurs in rivers with others of its kind. PERIODICITY: No known periodicity. MIGRATION PATTERNS: Non-migratory. COVER/SHELTER REQUIREMENTS: This species occurs in medium to large rivers with sand and gravel substrate (01,02). It apparently occurs in a range of water depths, since it has been hand collected and brail collected. It has been collected at a depth of 0.8 m in the Green River and roughly 1 m in the Clinch River (04). REPRODUCTIVE SITE REQUIREMENTS: P. plenum is a flowing water species that occurs in sand and gravel substrate in larger rivers (01). Since this species is believed to be sedentary and movement is inconsequential, this is the type of environment where all life history processes are carried out, including reproduction. REPRODUCTIVE CHARACTERISTICS: The reproductive cycle of P. plenum is presumed to be similar to other Pleurobema spp. (16,17). During spawning, males release sperm into the water column, and the sperm are taken in by the females during siphoning. Eggs are fertilized in the female's suprabranchial cavity or gills, which serve as marsupia for embryos developing to the parasitic stage (glochidia). Glochidia are released by the female and must attach to a suitable fish host for metamorphosis to the free-living juvenile stage. This species is probably a short-term breeder, based on gravid females collected in May (18). Spawning occurs in spring and glochidia are released in summer (01). Glochidia are probably semicircular and hookless (16,19). Fish hosts are unknown for this species, but fish hosts for P. cordatum and P. oviforme have been identified (14,16,17,20). PARENTAL CARE: Life History - 1 (DRAFT) - Life History Species PIGTOE, ROUGH Species Id ESIS404019 Date 13 MAR 96 Except for retention of the glochidia within the gills of the female prior to release, no parental care occurs. POPULATION BIOLOGY: No information exists on the population biology of this species. The species is apparently taken occasionally by commercial musselmen (01). SPECIES INTERRELATIONSHIPS: The required fish hosts for the parasitic glochidia of this species are unknown (01). The rosefin shiner (Notropis ardens) and the bluegill (Lepomis macrochirus) have indicated as fish hosts for P. cordatum (14,16,20). However, rare mussel species such as P. plenum may utilize a single fish host (04). OTHER LIFE HISTORY DESCRIPTORS: None. Life History - 2
                           (DRAFT) - Management Practices
                                Species PIGTOE, ROUGH
                                Species Id ESIS404019
                                   Date 13 MAR 96



MANAGEMENT PRACTICES

Management Practices - 1 (DRAFT) - Management Practices Species PIGTOE, ROUGH Species Id ESIS404019 Date 13 MAR 96 RESULT MANAGEMENT PRACTICE Beneficial Developing/maintaining stream structures Beneficial Maintaining/Controlling Water Flow Beneficial Controlling/Restricting Mining Beneficial Controlling/Restricting Road Maintainance Actions Beneficial Maintaining undisturbed/undeveloped areas Beneficial Land Acquisition Beneficial Controlling pollution [thermal, chemical, physical] Beneficial Controlling/Restricting Pesticide Use Beneficial Controlling/Restricting Herbicide Use Beneficial Controlling/Restricting Agricultural Practice Beneficial Stocking captive-reared wild-strain animals Beneficial Transplanting wild animals Beneficial Transplanting Wild Eggs/Wild Seeds Beneficial Controlling/Restricting Noncommercial Harvest Beneficial Regulating commercial harvest levels Adverse Incidental Capturing/Killing Existing Incidental Capturing/Killing Adverse Inherent Reproductive Characteristics Existing Inherent Reproductive Characteristics Adverse Low Gene Pool Existing Low Gene Pool Adverse Underground Mines Existing Underground Mines Adverse Surface Mines Existing Surface Mines Adverse Siltation Existing Siltation Adverse Water Temperature Alteration Existing Water Temperature Alteration Adverse Dissolved Oxygen Reduction Existing Dissolved Oxygen Reduction Adverse Water Level Fluctuation Existing Water Level Fluctuation Adverse Reservoirs Existing Reservoirs Adverse Migration barriers Existing Migration barriers Adverse Dredging Existing Dredging Adverse Applying herbicides Existing Applying herbicides Adverse Applying pesticides Existing Applying pesticides Adverse Environmental Contamination/Pollution Existing Environmental Contamination/Pollution Adverse Exotic/Feral/Introducted Species Existing Exotic/Feral/Introducted Species Adverse Existing Management Practices - 2 (DRAFT) - Management Practices Species PIGTOE, ROUGH Species Id ESIS404019 Date 13 MAR 96 COMMENTS ON MANAGEMENT PRACTICES - The mussel fauna in most streams of the Tennessee River drainage has been impacted by dam construction, channelization, siltation, and water pollution, which directly affect all mussel species (08). The change from lotic to lentic conditions because of dams has also altered the species composition of the fish community, thus jeopardizing the reproductive success of some mussel species by eliminating required fish hosts. The Green River dam, completed in 1969, may lead to the loss of that population. Although siltation has been suggested as the most significant adverse affect of impoundments on mussels (08), other detrimental factors include lowered water temperatures, pH changes, oxygen depletion, and dewatering of mussel beds below dams (08). Mussel deaths can occur in substrate on which 0.6 - 2.5 cm of silt has accumulated, resulting in interference with feeding and suffocation (09). The siphoning period and metabolic rate of mussels can also be affected by such contaminants as heavy metals and agricultural chemicals (10). Water pollution has probably contributed to the decline of this species. Acid mine drainage in tributaries of the Cumberland and Tennessee Rivers has reduced the diverse mussel assemblage that occurred there (11). Numerous tributaries in the upper Tennessee River drainage were polluted already by the twentieth century, and the mussel fauna was in a decline at that time (12). Coal waste from mining activities in both Tennessee and Cumberland River tributaries has contributed additional particulate and chemical pollutants. In the Green River, the freshwater mussel fauna has been almost totally eliminated below Greensburg, KY due to oil brine pollution (13). All of the factors mentioned above are still considered potential threats to remaining populations of P. plenum. Other factors that may also be affecting this species include incidental taking by commercial musselmen, invasion of the Asiatic clam (Corbicula fluminea), and the smaller gene pool which may be approaching the minimum population size needed for sufficient genetic variation to respond to environmental changes. Substantial mussel die-offs of unknown origin that have occurred since 1982 throughout the species' range also pose a direct threat to the species' survival. APPROVED PLAN: U.S. Fish and Wildlife Service. 1984. Rough Pigtoe Pearly Mussel Recovery Plan. U.S. Fish and Wildlife Service, Atlanta, GA. 51 pp. Recovery actions for the rough pigtoe include: (1) Continue to utilize existing legislation and regulations to protect the species and its habitat. (2) Conduct population and habitat surveys to determine the status and range of the species. (3) Determine present and forseeable threats to the species and strive to minimize and/or eliminate them. These negative impacts are Management Practices - 3 (DRAFT) - Management Practices Species PIGTOE, ROUGH Species Id ESIS404019 Date 13 MAR 96 likely to include coal mining and related pollutants, road construction, dredging, herbicide and pesticide spraying, industrial development, chlorinated effluents, the misuse of farm fertilizers, and oil and gas drilling. (4) Identify essential habitat in need or protection. Investigate the use of Scenic River status, mussel sanctuaries, land acquisition, and/or other means for the protection of this habitat and utilize where feasible. Habitat restoration or improvement may be necessary to improve conditions for survival of the species (i.e., restoration of stream banks and channels and assuring optimal clean water flow). (5) Determine the feasibility of introducing the species back into its historic range and introduce where feasible. Determine the best methods for establishing new populations (e.g., introduction of adult mussels, juveniles, infected fish hosts, artificially cultured individuals and/or other means. It is unlikely that removal from the list of Federal endangered and threatened species can be achieved without the establishment of populations in other rivers and the expansion of populations in rivers where it already occurs. (6) Control the unwarranted take of this species by commercial musselmen. Except for enforcement of regulations there are no recovery efforts presently underway for this species. Management Practices - 4
                                   (DRAFT) - References
                                  Species PIGTOE, ROUGH
                                  Species Id ESIS404019
                                      Date 13 MAR 96



     

References

***** REFERENCES FOR ALL NARRATIVES EXCEPT N-OCCURRENCE ***** 01 U.S. Fish and Wildlife Service. 1984. Recovery Plan for the Rough Pigtoe Pearly Mussel [Pleurobema plenum (Lea, 1840)]. U.S. Fish and Wildlife Service, Atlanta, GA. 51 pp. 02 Bogan, A.E. and P.W. Parmalee. 1983. Tennessee's rare wildlife volume II: the mollusks. Tenn. Wildl. Resour. Agency, Tenn. Dep. Conserv., and Tenn. Heritage Prog., Univ. Tenn., Knoxville, TN. 123 pp. 03 Simpson, C.T. 1914. A Descriptive Catalogue of the Naiades, or Pearly Freshwater Mussels. Bryant Walker, Detroit, MI. 1540 pp. 04 Clarke, A.H. 1981. Determination of the precise geographical areas occupied by four endangered species of freshwater mollusks. Final Rep. to U.S. Fish and Wildlife Service, Twin Cities, MN. 226 pp. 05 Stansbery, D.H. 1965. The naiad fauna of the Green River at Mumfordville, Kentucky, Am. Malac. Union Annu. Rep. Pp. 13-14. 06 Ortmann, A.E. and B. Walker. 1922. On the nomenclature of certain North American naiades. Occas. Pap. Mus. Zool. Univ. Mich. 112:1-75. 07 Parker, W. and L. Dixon. 1980. Endangered and threatened wildlife of Kentucky, North Carolina, South Carolina, and Tennessee. N.C. Agric. Ext. Serv. 116 pp. 08 Fuller, S.L.H. 1974. Clams and mussels (Mollusca:Bivalvia). Pages 215-273 In: C.W. Hart and S.L.H. Fuller (eds.), Pollution Ecology of Freshwater Invertebrates. Academic Press, NY. 09 Ellis, M.M. 1931. Some factors affecting the replacement of the commercial freshwater mussels. U.S. Bur. Fish. Circ. No.7. 10 pp. 10 Salanki, J. 1979. Behavioural studies in mussels under changing environmental conditions. Symp. Biol. Hung. 19:169-176. 11 Neel, J.K. and W.R. Allen. 1964. The mussel fauna of the upper Cumberland Basin before its impoundment. Malacologia 1:417-459. 12 Ortmann, A.E. 1918. The nayades (freshwater mussels) of the upper Tennessee drainage with notes on synonymy and distribution. Proc. Am. Phil. Soc., Phila. 57:521-626. 13 Williams, J.C. 1969. Mussel fishery investigation Tennessee, Ohio, and Green Rivers. Final Rep. to Ky. Dept. Fish. Wildl. Resour. Proj. No.4-19R. 107 pp. 14 Coker, R.E., A.F. Shira, H.W. Clark, and A.D. Howard. 1921. Natural history and propagation of fresh-water mussels. Bull. U.S. Bur. Fish. 37:77-181. 15 Churchill, E.P., Jr. and S.I. Lewis. 1924. Food and feeding in freshwater mussels. Bull. U.S. Bur. Fish. 39:439-471. 16 Yokley, P., Jr. 1972. Life history of Pleurobema cordatum (Rafinesque, 1820) (Bivalvia:Unionacea). Malacologia 11:351-364. 17 Weaver, L.R. 1981. Life history of Pleurobema oviforme (Mollusca: Unionidae) in Big Moccasin Creek, Virginia with emphasis on early life history, species associations, and age and growth. M.S. Thesis, VPI & SU, Blacksburg, VA. 89 pp. 18 Ortmann, A.E. 1919. A monograph of the naiades of Pennsylvania. Part 3. Systematic account of the genera and species. Mem. References - 1 (DRAFT) - References Species PIGTOE, ROUGH Species Id ESIS404019 Date 13 MAR 96 Carnegie Mus. 8:1-389. 19 Surber, T. 1915. Identification of the glochidia of freshwater mussels. Rep. U.S. Fish Comm. Appendix V:1-9. 20 Suber, T. 1913. Notes on the natural hosts of freshwater mussels. Bull. U.S. Bur. Fish. 32:110-116. ***** REFERENCES FOR N-OCCURRENCE NARRATIVE ONLY ***** 01 U.S. Fish and Wildlife Service. 1984. Recovery Plan for the Rough Pigtoe Pearly Mussel [Pleurobema plenum (Lea, 1840)]. U.S. Fish and Wildlife Service, Atlanta, GA. 51 pp. 02 Tennessee Valley Authority (TVA). 1979. Unpublished data. An evaluation of mussel populations in the Clinch River, Tennessee and Virginia. Div. Water Resour., Fish Aquat. Ecol. Branch, Norris, TN. 14 pp. 03 Parmalee, P.W., W.E. Klippel, and A.E. Bogan. 1980. Notes on the prehistoric and present status of the naiad fauna of the middle Cumberland River, Smith County, Tennessee. Nautilus 94:93-105. 04 Clarke, A.H. 1981. Determination of the precise geographical areas occupied by four endangered species of freshwater mollusks. Final Rep. to U.S. Fish and Wildlife Service, Twin Cities, MN. 226 pp. 05 Van der Schalie, H. 1939. Medionidus mcglameriae, a new naiad from the Tombigbee River, with notes on other naiads of that drainage. Occas. Pap. Mus. Zool., Univ. Mich. 407:1-6. 06 Lewis, J. 1876. Fauna of alabama. Freshwater and land snails. Ala. Geol. Surv. Prog. Rep. Pp. 61-100. 07 Ortmann, A.E. 1918. The nayades (freshwater mussels) of the upper Tennessee drainage with notes on synonymy and distribution. Proc. Am. Phil. Soc., Phila. 57:521-626. 08 Ortmann, A.E. 1919. A monograph of the naiades of Pennsylvania. Part 3. Systematic account of the genera and species. Mem. Carnegie Mus. 8:1-389. 09 Wilson, C.B. and H.W. Clark. 1914. The mussels of the Cumberland River and its tributaries. U.S. Bur. Fish. Doc. No.781. 63 pp. 10 Simpson, C.T. 1914. A Descriptive Catalogue of the Naiades, or Pearly Freshwater Mussels. Bryant Walker, Detroit, MI. 1540 pp. 11 Ortmann, A.E. 1911. Monograph of the naiades of Pennsylvania. Mem. Carnegie Mus. 4:279-347. 12 Ortmann, A.E. 1912. Notes upon the families and genera of the nayades. Ann. Carnegie Mus. 8:222-365. 13 La Rocque, A. 1966-1970. Pleistocene mollusca of Ohio. Ohio Div. Geol. Surv. Bull. 62. 14 Stansbery, D.H. 1972. A preliminary list of the naiad shells recovered from the Buffalo site. Pages 105-106. IN: B.J. Broyles, A late 17th century indian village site (46 Pu 31) in Putnam County, West Virginia. Rep. Archaeol. Invest. No.5, WV. Geol. Econ. Surv., Morgantown, WV. 15 Ortmann, A.E. 1926. The naiades of the Green River drainage in Kentucky. Ann. Carnegie Mus. 17:167-188. 16 Baker, F.C. 1906. A catalogue of the mollusca of Illinois. Bull. Ill. State Lab. Nat. Hist. 7:53-136. References - 2 (DRAFT) - References Species PIGTOE, ROUGH Species Id ESIS404019 Date 13 MAR 96 17 Baker, F.C. 1905. The mollusca fauna of McGregor, Iowa. Trans. Acad. Sci. St. Louis. 15:249-258. 18 Danglade, E. 1914. The mussel resources of the Illinois River. U.S. Bur. Fish., Rep. U.S. Comm. Fish 1913. 48 pp. 19 Call, R.E. 1895. The Unionidae of Arkansas. Trans. Acad. Sci. St. Louis. 20 Grier, N.M. 1915. List of the naiads of the Meramec River, Missouri. Am. Midl. Nat. 32:257-326. 21 Utterback, W.I. 1915-1916. The naiades of Missouri. Am. Midl. Nat. 4:41-53, 97-152, 181-204, 244-273, 311-327, 339-354, 387-400, 432-464. References - 3