(DRAFT) - Taxonomy
                           Species AMPHIPOD, HAY'S SPRING
                                Species Id ESIS455001
                                   Date 13 MAR 96



TAXONOMY

NAME - AMPHIPOD, HAY'S SPRING OTHER COMMON NAMES - AMPHIPOD, HAY'S SPRING; SCUD and HAY'S SPRING ELEMENT CODE - CATEGORY - Aquatic Crustaceans PHYLUM AND SUBPHYLUM - ARTHROPODA, CLASS AND SUBCLASS - CRUSTACEA, ORDER AND SUBORDER - AMPHIPODA, FAMILY AND SUBFAMILY - CRANGONYCTIDAE, GENUS AND SUBGENUS - STYGOBROMUS, SPECIES AND SSP - HAYI, SCIENTIFIC NAME - STYGOBROMUS HAYI AUTHORITY - TAXONOMY REFERENCES - COMMENTS ON TAXONOMY - Hay's Spring Amphipod Stygobromus hayi (Hubricht and Mackin, 1940) KINGDOM: Animal GROUP: Crustacean PHYLUM: Arthropoda CLASS: Crustacea ORDER: Amphipoda FAMILY: Crangonyctidae Stygobromus hayi (Hay's Spring amphipod) is an eyeless, unpigmented gammaridean amphipod crustacean of typical crangonyctid facies (01,02). The third uropod is greatly reduced, lateral sternal gills are bifurcate and the uronites are fused (02). The largest known females measure approximately 10 mm; largest males are 9.75 mm. When first described, this species was placed in the genus Synpleonia by Hubricht and Mackin (09). It was later erroneously listed from two caves in Pennsylvania by Hubricht (02,07). In 1959, Hubricht synonymized this species with Synpleonia tenuis (02,08). It was resurrected from synonymy and redescribed by Holsinger in 1967 and assigned to the genus Stygonectes (02). Later, it was transferred to the genus Stygobromus by Holsinger (03). A series of ten cotypes (syntypes) are deposited in the National Museum of Natural History of the Smithsonian Institution under catalog numbers of the United States National Museum (USNM 77804). Additional Taxonomy - 1 (DRAFT) - Taxonomy Species AMPHIPOD, HAY'S SPRING Species Id ESIS455001 Date 13 MAR 96 paratypes are also in the Smithsonian collections. Six topotypes, collected 7 April 1978, are in the collection of J.R. Holsinger at Old Dominion University (H-1886). Other topotypes presumably collected by Drs. J.L. Barnard and G.S. Karaman are also probably in the Smithsonian collections. Another common name for this species is Hay's Spring scud (05,06, 10). Taxonomy - 2
                                  (DRAFT) - Status
                           Species AMPHIPOD, HAY'S SPRING
                                Species Id ESIS455001
                                   Date 13 MAR 96



STATUS

Coded Status E: Federal Endangered COMMENTS ON STATUS - U.S. STATUSES AND LAWS: Hay's Spring amphipod (Stygobromus hayi) has been designated an Endangered species pursuant to the Endangered Species Act of 1973 (50 CFR 17.11; P.L. 93-205, 87 Stat. 884; 16 U.S.C. 1531-1540), as amended. The species has this status wherever found including the District of Columbia. This species is protected by the Lacey Act (P.L. 97-79, as amended; 16 U.S.C. 3371 et seq.) which makes it unlawful to import, export, transport, sell, receive, acquire, or purchase any wild animal (alive or dead including parts, products, eggs, or offspring): (1) in interstate or foreign commerce if taken, possessed, transported or sold in violation of any State law or regulation; or (2) if taken or possessed in violation of any U.S. law, treaty, or regulation or in violation of Indian tribal law. It is also unlawful to possess any wild animal (alive or dead including parts, products, eggs, and offspring) within the U.S. territorial or special maritime jurisdiction (as defined in 18 U.S.C. 7) that is taken, possessed, transported, or sold in violation of any State law or regulation, foreign law, or Indian tribal law. RESPONSIBLE FEDERAL AGENCIES: USFWS -Responsible for the management/recovery, listing, and law enforcement/protection of this species. All Federal agencies have responsibility to ensure that any action authorized, funded, or carried out by that agency is not likely to jeopardize the continued existence of the species or result in the destruction or adverse modification of Critical Habitat (50 CFR 402), and to utilize their authorities to carry out programs for the conservation of the species. STATE STATUSES AND LAWS: STATE: District of Columbia DESIGNATED STATUS: None INTERNATIONAL STATUSES, TREATIES, AND AGREEMENTS: None. ECONOMIC STATUSES: None. Status - 1 (DRAFT) - Status Species AMPHIPOD, HAY'S SPRING Species Id ESIS455001 Date 13 MAR 96 77/01/12:42 FR 02507/02515 - Proposal to list as Endangered 79/12/10:44 FR 70796/70797 - Proposal withdrawn 80/07/25:45 FR 49850/49851 - Reproposal to list as Endangered 82/02/05:47 FR 05425/05427 - Final rule, list as Endangered 87/07/07:52 FR 25523/25528 - Notice of five year review Status - 2
     

HABITAT ASSOCIATIONS

HABITAT - AQUATIC INLAND AQUATIC LAND USE - Commercial and Services Transportation, communications, and Util Mixed Urban or Built-up Land Deciduous Forest Land Streams and Canals COMMENTS ON HABITAT ASSOCIATIONS - The habitat of Stygobromus hayi (Hay's Spring amphipod) consists of a small spring, possibly permanent but seep-like in appearance within a densely populated urban area, the National Zoological Park, Washington, D.C.. A short section of the spring runs immediately in front of the resurgence itself (04). The spring appears to issue forth from crevices in Precambrian rocks of the Piedmont province (02). The amphipods occur in decaying deciduous leaf litter and mud at the spring exit. They are associated with epigean amphipods, Crangonyx (near pseudogracilis), asellid isopods, Caecidotea kenki, and oligochaetes. Crangonyx and Caecidotea were much more common when observed by Holsinger on 7 April 1978 (04). The original collection of S. hayi by Leslie Hubricht in March 1938 contained a single, small specimen of another stygobiont amphipod, Stygobromus tenuis, a species common to groundwater outlets throughout much of the greater Washington, D.C. area (02,03). Stygobromus tenuis was not found here by sampling in April 1978 (04). High flood levels from Rock Creek may adversely affect the spring habitat by removing individual amphipods as well as the leaves and soft bottom sediments that form their microhabitat (11). The spring water is cool and of typical groundwater temperature for this region (ca. 10-13 deg. C), fresh, and not obviously polluted. Other physical/chemical parameters have not been measured (04). Most of the surrounding surface vegetation consists of a variety of deciduous trees and various small plants (04). It should be noted that only a small percentage of the actual population of this species is probably seen in the spring habitat itself (04). Given that S. hayi is of stygobiont facies (i.e., eyeless and unpigmented), it probably inhabits cracks, crevices and interstitial spaces of the mantle/bedrock area that forms the recharge zone for the spring (02). The full range of this species may extend to the limits of this groundwater aquifer, but the extent of the aquifer is unknown (04). Habitat Associations - 1
                                (DRAFT) - Food Habits
                           Species AMPHIPOD, HAY'S SPRING
                                Species Id ESIS455001
                                   Date 13 MAR 96



FOOD HABITS

TROPHIC LEVEL - HERBIVORE LIFESTAGE FOOD FOOD PART General Detritus General Bacteria General Myxomycophyta General Deciduous Trees-Leaves/Twigs Food Habits - 1
                         (DRAFT) - Environment Associations
                           Species AMPHIPOD, HAY'S SPRING
                                Species Id ESIS455001
                                   Date 13 MAR 96



ENVIRONMENTAL ASSOCIATIONS

G = General A = Adult LIM = Limiting RA = Resting Adult J = Juvenile FA = Feeding Adult RJ = Resting Juvenile BA = Breeding Adult FJ = Feeding Juvenile P = Pupae L = Larvae E = Egg RL = Resting Larvae FL = Feeding Larvae
LIFESTAGE ENVIRONMENTAL ASSOCIATIONS G Aquatic Features: Springs [flowing] Environment Associations - 1
                               (DRAFT) - Life History
                           Species AMPHIPOD, HAY'S SPRING
                                Species Id ESIS455001
                                   Date 13 MAR 96



LIFE HISTORY

FOOD HABITS: Nothing definitive is currently known about the food requirements or preferences of Stygobromus hayi (Hay's Spring amphipod). Considering its association with decaying deciduous leaves and organically enriched mud, it is assumed that S. hayi feeds on leaves, decaying organic detritus, and decomposer microorganisms (e.g., bacteria and fungi) which occur on the organic material (04). Most freshwater amphipods are believed to be primarily herbivores, and there is no evidence to the contrary for S. hayi (04). HOME RANGE/TERRITORY: This is not applicable to S. hayi (04). PERIODICITY: No periodicity has been observed for S. hayi (04). MIGRATION PATTERNS: Stygobromus hayi is nonmigratory (04). COVER/SHELTER REQUIREMENTS: Stygobromus hayi is found in a type of groundwater habitat called the hypotelminorheic, which can be defined as the outflow of a small spring or seep through fine sediments or leaf litter (03,04). Wet mud and decaying leaves offer cover and shelter necessary for the survival of this species in the habitat where it is found (04). However, probably a much greater number of animals live in the subterranean groundwater aquifer that feeds the spring but which is inaccessible to direct observation (04). This conjectured subterranean habitat probably consists of cracks, crevices and interstitial spaces saturated with groundwater (02,03). Therefore, the watershed behind the spring must be protected in order to insure continued survival of this species (04). REPRODUCTIVE SITE REQUIREMENTS: It is assumed that the present spring habitat and the associated groundwater aquifer provide the reproductive site requirements for this species (04). REPRODUCTIVE CHARACTERISTICS: Nothing definitive is currently known about breeding or reproduction in this species (02,03,04). Of the 27 or 28 specimens examined by Holsinger, approximately 12 were females, 12 were males, and three were juveniles (04). Females brooding eggs or embryos have not been observed to date, although a single female, 8.75-9.0 mm long, from a collection made by Leslie Hubricht on 9 March 1938, had setose brood plates. Presumably, females are sexually mature at this size, because setose brood plates (oostegites) form the brood pouch for eggs following fertilization. Juveniles were present in the 9 March 1938 and 7 April 1978 samples, but whether they occur in seasons other than spring is unknown (samples have been taken only in March and April) (04). Life History - 1 (DRAFT) - Life History Species AMPHIPOD, HAY'S SPRING Species Id ESIS455001 Date 13 MAR 96 PARENTAL CARE: No data are available on parental care, although like all other amphipods, S. hayi females undoubtedly brood their eggs in pouches formed from oostegites on the ventral side (04). POPULATION BIOLOGY: Because of very limited sampling and the small accessible population, there are no data available on population biology of this species (02,03,04). SPECIES INTERRELATIONSHIPS: No information is available. OTHER LIFE HISTORY DESCRIPTORS: No information is available. Life History - 2
                           (DRAFT) - Management Practices
                           Species AMPHIPOD, HAY'S SPRING
                                Species Id ESIS455001
                                   Date 13 MAR 96



MANAGEMENT PRACTICES

RESULT MANAGEMENT PRACTICE Beneficial Restricting/regulating human disturbance of populations Beneficial Maintaining/Controlling Water Flow Beneficial Maintaining undisturbed/undeveloped areas Beneficial Controlling/Restricting Pesticide Use Adverse Collecting Existing Collecting Adverse Recreational development Existing Recreational development Adverse Soil compaction by heavy equipment in mine areas Existing Soil compaction by heavy equipment in mine areas Adverse Siltation Existing Siltation Adverse Draining wetlands, marshes, ponds, lakes Existing Draining wetlands, marshes, ponds, lakes Adverse Flooding Existing Flooding Adverse Environmental Contamination/Pollution Existing Environmental Contamination/Pollution COMMENTS ON MANAGEMENT PRACTICES - Reasons for the current Endangered status of Stygobromus hayi (Hay's Spring amphipod) are given as follows: 1) Vulnerability to extinction by its extremely restricted distribution. 2) Proximity of its habitat (a single spring) to an area used in the past (before 1982) for the disposal of fill from construction activities in the National Zoological Park. 3) Accidential destruction of the spring habitat during material disposal or other operations on an adjacent area just south of the South Gate to the park. 4) Overcollecting. 5) High flood levels from Rock Creek may adversely affect the spring habitat by removing individual amphipods as well as the leaves and soft bottom sediments that form their microhabitat. There is also a possibility that Rock Creek will become polluted with sewage effluent and overflow onto the spring during a major flood (04). Surrounding urban and commercial areas that include highways, utilities, etc. pose a current threat to this species. A potential future threat may also be from urban runoff threatening the water quality within the aquifer. Officials of the National Zoological Park have a Memorandum of Understanding with the Fish and Wildlife Service that addresses concerns 1 through 4 above. They have agreed to perform all things necessary for or incidental to the protection of S. Hayi. A copy of the MOU is on file with the Fish and Wildlife Service and the National Zoo. Management Practices - 1 (DRAFT) - Management Practices Species AMPHIPOD, HAY'S SPRING Species Id ESIS455001 Date 13 MAR 96 The status of proposals in 1978 for discharge of treated sewage into Rock Creek by the Washington Suburban Sanitary Commission, and similar discharge into the Creek by a sewage treatment plant upstream in Montgomery County, Maryland (see 05,06) are unknown. UNAPPROVED PLAN: No recovery plan or target date has yet been set for initiation of a formal recovery plan. A Memorandum of Understanding has been entered into by the National Zoological Park and the U.S. Fish and Wildlife Service to implement recovery actions for the Hay's Spring amphipod (Stygobromus hayi). Recommended recovery actions include: protection of the only known habitat site (on National Zoological Park property) by limiting human access and insuring that park maintenance or other activities do not disturb the species' habitat (e.g., pesticide spraying or development of the spring habitat); and monitoring of water quality in the spring occupied by the amphipod. Ongoing and completed recovery actions include: fencing and gating by the Smithsonian Institution (National Zoological Park) to prevent human access; and periodic monitoring of the habitat to insure that it remains undisturbed. Management Practices - 2
                                   (DRAFT) - References
                              Species AMPHIPOD, HAY'S SPRING
                                  Species Id ESIS455001
                                      Date 13 MAR 96



     

References

***** REFERENCES FOR ALL NARRATIVES EXCEPT N-OCCURRENCE ***** 01 Garrison, B. 1981. Our very own endangered species. Washington Post Magazine, Feb. 8, 1981. Pages 14-16. 02 Holsinger, J.R. 1967. Systematics, speciation and distribution of the subterranean amphipod genus Stygonectes (Gammaridae). U.S. Natl. Mus. Bull. 259:1-176. 03 Holsinger, J.R. 1978. Systematics of the subterranean amphipod genus Stygobromus (Crangonyctidae). Part II: Species of the eastern United States. Smithsonian Contrib. Zool. 266:1-144. 04 Holsinger, J.R. 1985. Data in research files. Dept. Biol. Sci., Old Dominion Univ., Norfolk, VA 23508. 05 Holt, B. 1978. Hay's Spring scud vs. sewage plan. Washington Star, 6 May 1978. Pages A-1,A-6. 06 Holt, B. 1978. Hay's spring scud are headed for endangered species list. The Washington Star, 16 May 1978. Pages B-1,B-2. 07 Hubricht, Leslie. 1943. Studies on the Nearctic freshwater Amphipoda, III: Notes on the freshwater Amphipoda of eastern United States with description of ten new species. Am. Mid. Nat. 29:683-712. 08 Hubricht, L. 1959. Malacostraca (Amphipoda). Pages 876-878. IN: Freshwater biology (2nd. Ed.). W.T. Edmondson, ed. Wiley & Sons, Inc., New York. 09 Hubricht, L. and J.G. Mackin. 1940. Description of nine new species of fresh-water amphipoda crustaceans with notes and new localities for other species. Am. Mid. Nat. 23:187-218. 10 Jacobs, Madeleine. 1982. Scud officially `endangered'. The Torch No. 82 (4 April 1982). Pages 1,4. 11 USFWS. Febuary 5, 1982. Endangered and threatened wildlife and plants; LIsting Hay's Springs amphipods as an Endangered species. FEd. Reg. 47(25):5425-5426. ***** REFERENCES FOR N-OCCURRENCE NARRATIVE ONLY ***** 01 Holsinger, J.R. 1967. Systematics, speciation and distribution of the subterranean amphipod genus Stygonectes (Gammaridae). U.S. Natl. Mus. Bull. 259:1-176. 02 Holsinger, J.R. 1978. Systematics of the subterranean amphipod genus Stygobromus (Crangonyctidae). Part II: Species of the eastern United States. Smithsonian Contrib. Zool. 266:1-144. 03 Holsinger, J.R. 1985. Data in research files. Department of Biological Sciences, Old Dominion University, Norfolk, VA 23508. References - 1