(DRAFT) - Taxonomy
                            Species ISOPOD, MADISON CAVE
                                Species Id ESIS455002
                                   Date 13 MAR 96



TAXONOMY

NAME - ISOPOD, MADISON CAVE OTHER COMMON NAMES - ISOPOD and MADISON CAVE ELEMENT CODE - CATEGORY - Aquatic Crustaceans PHYLUM AND SUBPHYLUM - ARTHROPODA, CLASS AND SUBCLASS - CRUSTACEA, ORDER AND SUBORDER - ISOPODA, FAMILY AND SUBFAMILY - CIROLANIDAE, GENUS AND SUBGENUS - ANTROLANA, SPECIES AND SSP - LIRA, SCIENTIFIC NAME - ANTROLANA LIRA AUTHORITY - TAXONOMY REFERENCES - COMMENTS ON TAXONOMY - Madison Cave Isopod Antrolana lira Bowman, 1964 KINGDOM: Animal GROUP: Crustacean PHYLUM: Arthropoda CLASS: Crustacea ORDER: Isopoda FAMILY: Cirolanidae Antrolana lira (Madison Cave isopod) was first described by Bowman in 1964. This species is an isopod crustacean of typical cirolanid facies (01). The body is flattened, compact, and approximately three times longer than broad. The species lacks eyes and pigment. The largest specimens measure 21 mm, but most of the animals observed are between 9 and 16 mm (02). The male holotype (USNM 110783) and six paratypes (USNM 110785) from Madison's Saltpetre Cave are deposited in the National Museum of National History of the Smithsonian Institution under catalog numbers of the United States National Museum (USNM) (01). Numerous additional specimens, mostly voucher specimens from the Collins thesis study (02,03) are in the collections of John R. Holsinger at Old Dominion University, VA. Taxonomy - 1
                                  (DRAFT) - Status
                            Species ISOPOD, MADISON CAVE
                                Species Id ESIS455002
                                   Date 13 MAR 96



STATUS

Coded Status Virginia; State Recognized T: Federal Threatened COMMENTS ON STATUS - U.S. STATUSES AND LAWS: Madison Cave isopod (Antrolana lira) has been designated a Threatened species pursuant to the Endangered Species Act of 1973 (50 CFR 17.11; P.L. 93-205, 87 Stat. 884; 16 U.S.C. 1531-1540), as amended. The species has this status wherever found including the State of Virginia. Special rules concerning take for scientific purposes for this species can be found in 50 CFR 17.46(a). This species is protected by the Lacey Act (P.L. 97-79, as amended; 16 U.S.C. 3371 et seq.) which makes it unlawful to import, export, transport, sell, receive, acquire, or purchase any wild animal (alive or dead including parts, products, eggs, or offspring): (1) in interstate or foreign commerce if taken, possessed, transported or sold in violation of any State law or regulation; or (2) if taken or possessed in violation of any U.S. law, treaty, or regulation or in violation of Indian tribal law. It is also unlawful to possess any wild animal (alive or dead including parts, products, eggs, and offspring) within the U.S. territorial or special maritime jurisdiction (as defined in 18 U.S.C. 7) that is taken, possessed, transported, or sold in violation of any State law or regulation, foreign law, or Indian tribal law. RESPONSIBLE FEDERAL AGENCIES: USFWS -Responsible for the management/recovery, listing, and law enforcement/protection of this species. All Federal agencies have responsibility to ensure that any action authorized, funded, or carried out by that agency is not likely to jeopardize the continued existence of the species or result in the destruction or adverse modification of Critical Habitat (50 CFR 402), and to utilize their authorities to carry out programs for the conservation of the species. STATE STATUSES AND LAWS: STATE: Virginia DESIGNATED STATUS: Threatened ADMINISTRATIVE AGENCY: Virginia Commission of Game and Inland Fisheries STATE STATUTE: State Code 29-230 to 29-237 (Chapter 11 - Status - 1 (DRAFT) - Status Species ISOPOD, MADISON CAVE Species Id ESIS455002 Date 13 MAR 96 Endangered Species) INTERNATIONAL STATUSES, TREATIES, AND AGREEMENTS: The Madison Cave isopod is listed as Vulnerable in the IUCN Red Data Book, 1983. ECONOMIC STATUSES: None. 77/01/12:42 FR 02507/02515 - Proposal to list as Threatened 79/12/10:44 FR 70796/70797 - Proposal withdrawn 80/10/06:45 FR 66410/66411 - Reproposal to list as Threatened 82/10/04:47 FR 43699/43701 - Final rule, list as Threatened 87/07/07:52 FR 25523/25528 - Notice of five year review Status - 2
     

HABITAT ASSOCIATIONS

HABITAT - AQUATIC INLAND AQUATIC SOCIETY OF AMERICAN FORESTRY TYPES SAF TYPE STAGE CLOSURE Shortleaf Pine-Oak mature tree LAND USE - Mixed Forest Land Lakes COMMENTS ON HABITAT ASSOCIATIONS - The accessible physical habitat of the Madison Cave isopod (Antrolana lira) consists of three lakes of deep phreatic water (i.e., below the level of the water table) which occupy narrow cave passages developed along bedding planes in limestone strata dipping ca. 67 deg. NW (03). The surface of these lakes is believed to represent the ground water table beneath Cave Hill (03,06). The east and west lakes are accessible from the lower level of Madison's Saltpetre Cave and have depths of 21.5 m and 10.7 m respectively (02,03,06). The lake in Steger's Fissure is ca. 30.5 m deep and consumes nearly all of this fissure-cave (02,03,06). The fissure lake is 165 m NNE of the cave lakes. All three lakes are believed to share the same body of phreatic water associated with the Cave Hill subterranean aquifer (02, 06). Only one other aquatic species (the troglobitic amphipod crustacean Stygobromus stegerorum Holsinger, 1978) has been found to co-occur with A. lira in the cave lakes. This same amphipod and an unidentified flatworm occur with A. lira in the fissure lake (06). Observed physical/chemical parameters of the lake habitats include: fresh water; temperature of 11-14 deg. C; dissolved oxygen of 9.3-9.4 mg/l; nitrate/nitrite of 1.6 mg.; and low turbidity (02). There is a sinkhole on Cave Hill near the town of Grottoes, VA, above the cave that is believed to be one of several important recharge points for the Cave Hill subterranean aquifer and a principal entry point for nutrients. A water storage facility was built on Cave Hill and improper management or maintenence could pose a future threat to the species. Some of these concerns are salting of an access road to the tank site, discharge of paint or other chemicals used to maintain the tank, erosion from improper drainage along the access road or at the tank site, and further cutting of trees and other vegetation in the vicinity of the tank (06). The caves are located just west of South River. Water level fluctuations in South River could impact the local ground water taken in the aquifer (06). Habitat Associations - 1
                                (DRAFT) - Food Habits
                            Species ISOPOD, MADISON CAVE
                                Species Id ESIS455002
                                   Date 13 MAR 96



FOOD HABITS

TROPHIC LEVEL - HERBIVORE LIFESTAGE FOOD FOOD PART General Detritus General Bacteria General Myxomycophyta General Deciduous Shrubs-Leaves/Twigs General Deciduous Trees-Leaves/Twigs General Arthropods Food Habits - 1
                         (DRAFT) - Environment Associations
                            Species ISOPOD, MADISON CAVE
                                Species Id ESIS455002
                                   Date 13 MAR 96



ENVIRONMENTAL ASSOCIATIONS

G = General A = Adult LIM = Limiting RA = Resting Adult J = Juvenile FA = Feeding Adult RJ = Resting Juvenile BA = Breeding Adult FJ = Feeding Juvenile P = Pupae L = Larvae E = Egg RL = Resting Larvae FL = Feeding Larvae
LIFESTAGE ENVIRONMENTAL ASSOCIATIONS G Terrestrial Features: Caves, wet G Terrestrial Features: Depressions Environment Associations - 1
                               (DRAFT) - Life History
                            Species ISOPOD, MADISON CAVE
                                Species Id ESIS455002
                                   Date 13 MAR 96



LIFE HISTORY

FOOD HABITS: Specific food items eaten by Antrolana lira (Madsion Cave isopod) are unknown. However, based on preliminary gut content analysis, it is believed that this species feeds on decaying organic matter consisting of deciduous leaf litter, twigs and other wood particles (02). Parts from dead insects, presumably from decomposition of epigean insects that wash into the aquifer, are also eaten (02). It is also likely that this species feeds on bacteria, fungi and other microorganisms associated with the organic matter. HOME RANGE/TERRITORY: This is not applicable to Antrolana lira. PERIODICITY: No periodicity has been observed in Antrolana lira (06). MIGRATION PATTERNS: Antrolana lira is nonmigratory (06). COVER/SHELTER REQUIREMENTS: This species inhabits lakes of phreatic water (i.e., below the level of the water table), with lake depth ranging from ca. 10 to 30 meters, that are still, clear and receive some influx of organic materials. The species is significantly more abundant in the two deeper lakes (i.e., East Lake in Madison's Saltpetre Cave and a lake in Steger's Fissure) (02,03). Availability of food may have as much or more to do with abundance as water depth (06). REPRODUCTIVE SITE REQUIREMENTS: Because ovigerous (egg-bearing) females have not been found to date, nothing can be said about where in the Cave Hill groundwater aquifer this species reproduces (02,03,06). REPRODUCTIVE CHARACTERISTICS: Nothing is known about the life span or age when breeding occurs (02). Although specimens can be sexed at ca. 9.7 mm in length, a definitive sexual maturity for females is undetermined because females brooding eggs or embryos have not been found to date (02,03,06). However, a few females containing spherical structures thought to be oocytes ranged in size between 13.3 and 21.0 mm. Based on these preliminary data, females may reach sexual maturity at approximately 13 mm (02). PARENTAL CARE: Presumably, like many other isopod crustaceans, this species broods its eggs in a brood pouch formed from oostegites on the ventral side. However, females brooding eggs or embryos have not been found to date, suggesting that females are secretive while reproducing and remain deep within the lakes (perhaps in mud/silt on the bottom) or for some reason do not brood their eggs in the conventional manner (02,03,06). Life History - 1 (DRAFT) - Life History Species ISOPOD, MADISON CAVE Species Id ESIS455002 Date 13 MAR 96 POPULATION BIOLOGY: Data on population biology for Antrolana lira are based on monthly and seasonal sampling for a one-year period using submerged jars baited with fresh shrimp (02,03). These data, although preliminary, suggest that A. lira may reproduce more or less continually at a low rate without apparent peaks or cycles. Until brooding females are found, however, nothing more definitive can be stated. Population structures based on these samples suggest: (1) females outnumber males; (2) a skewing towards adult size classes (e.g., 9-16 mm); and (3) relatively small population size (the largest number of organisms observed in a monthly sample was 548, but fewer than 100 per monthly sample was far more common). SPECIES INTERRELATIONSHIPS: No information is available. OTHER LIFE HISTORY DESCRIPTORS: No information is available. Life History - 2
                           (DRAFT) - Management Practices
                            Species ISOPOD, MADISON CAVE
                                Species Id ESIS455002
                                   Date 13 MAR 96



MANAGEMENT PRACTICES

RESULT MANAGEMENT PRACTICE Beneficial Restricting/regulating human disturbance of populations Beneficial Controlling water levels Beneficial Controlling/Restricting Road Maintainance Actions Beneficial Maintaining undisturbed/undeveloped areas Beneficial Controlling pollution [thermal, chemical, physical] Beneficial Controlling/Restricting Pesticide Use Beneficial Controlling/Restricting Herbicide Use Adverse Incidental Capturing/Killing Existing Incidental Capturing/Killing Adverse Harassment/Vandalism/Indiscriminate Killing Existing Harassment/Vandalism/Indiscriminate Killing Adverse Collecting Existing Collecting Adverse Spelunking Existing Spelunking Adverse Soil compaction by heavy equipment in mine areas Existing Soil compaction by heavy equipment in mine areas Adverse Siltation Existing Siltation Adverse Strip mining Existing Strip mining Adverse Environmental Contamination/Pollution Existing Environmental Contamination/Pollution Adverse Erosion Existing Erosion Adverse Harvesting Existing Harvesting COMMENTS ON MANAGEMENT PRACTICES - The major reasons used to justify the threatened status of Antrolana lira (Madison Cave isopod) are: (1) Its highly localized range; (2) unique zoogeography; (3) rarity; (4) proximity of habitat to the South River which was determined in 1977 to be polluted by mercury; and (5) potential vandalism and/or disturbance of habitat and indiscriminate collecting by "uneducated" spelunkers (04,05,06). Other possible threats to this species include: (1) forest clearing or alteration on the hill above the caves that may have, or would, affect the flow of water and nutrients into the sinkhole recharge zone of the groundwater aquifer; (2) alteration of surface drainage which might speed up erosion and runoff into sinkholes and result in siltation of the aquifer; and (3) use of equipment in construction on Cave Hill (06). The threat of habitat disturbance (or vandalism) and indiscriminate collecting was essentially eliminated in 1981 when the entrance to Madisons Saltpetre Cave was gated by responsible cavers and conservationists. Access to this cave is now rigorously controlled through a conservation-management agreement between the cave owner, Mr. Lyall O. Steger, Jr. and the Cave Conservancy of the Management Practices - 1 (DRAFT) - Management Practices Species ISOPOD, MADISON CAVE Species Id ESIS455002 Date 13 MAR 96 Virginias (06). Steger's Fissure, a cave-like fissure near the entrance to Madison's Saltpetre Cave, is unprotected by a gate. Recently, a possible threat to the Madisons Cave isopod was the construction of a water storage tank on Cave Hill by the town of Grottoes in 1983. During construction work, which was supposed to have taken place under strict conservation guidelines established by the Virginia Cave Commission, dirt and rocks were dumped into a large sinkhole near the tank site on the hill above the caves. Because this sinkhole is believed to be one of several important recharge points for the Cave Hill subterranean aquifer and a principal entry point for nutrients, the town was asked to remove the fill immediately. After months of negotiations, the fill was removed and the sinkhole was restored to its near natural state in early 1984. As of the end of 1985, there has been no noticeable adverse effect on the populations of Antrolana lira (06). However, improper management or maintenance of the water storage facility could pose a threat to the species. Some of these concerns are salting of the access road to the tank site, discharge of paint or other chemicals used to maintain the tank, erosion from improper drainage along the access road or at the tank site, and further cutting of trees and other vegetation in the vicinity of the tank. Any significant future alteration of present surface conditions on Cave Hill above the caves, such as clearing of forests, filling of sinkholes, or renewed construction around the water storage facility, could conceivably adversely affect the underlying aquifer through erosion, siltation, interference with surface to groundwater flow of nutrients, and change of water table level (06). Water level fluctuation in South River which could significantly lower the local groundwater table below a critical point for survival of the populations in the aquifer is a potential future threat (06). Thorough documentation of the Grottoes water tank incident can be found in the files of Dr. J.R. Holsinger at Old Dominion University (06). UNAPPROVED PLAN: No recovery plan for the Madison Cave isopod has been initiated; no target date has been set for initiation. Recommended recovery actions for the Madsion Cave isopod (Antrolana lira) include protection of the two known habitat sites (Madison Cave and Stegers Fissure) from excessive human entry and contamination of the groundwater system feeding the underground lakes. Monitoring of water quality and the water table in the cave and fissure must be implemented so that management guidelines for each habitat site can be developed. Management guidelines might include control of herbicide and pesticide use in the surrounding area; control of pollutants dumped into the South River; restricting road maintenance practices (salting, improper drainage, and removal of vegetation); control of development in the area surrounding the site; and maintaining the water table so that the local groundwater table does not fall below a critical point for survival of the populations in the aquifer. Management Practices - 2 (DRAFT) - Management Practices Species ISOPOD, MADISON CAVE Species Id ESIS455002 Date 13 MAR 96 Ongoing recovery actions consist of periodically monitoring Madison cave isopod populations. Management Practices - 3
                                   (DRAFT) - References
                               Species ISOPOD, MADISON CAVE
                                  Species Id ESIS455002
                                      Date 13 MAR 96



     

References

***** REFERENCES FOR ALL NARRATIVES EXCEPT N-OCCURRENCE ***** 01 Bowman, T.E. 1964. Antrolana lira, a new genus and species of troglobitic cirolanid isopod from Madison Cave, Virginia. Internat. J. Speleology 1(1+2):229-236. 8 plts. 02 Collins, T.L. 1982. An ecological study of the troglobitic cirolanid isopod, Antrolana lira Bowman, from Madisons Saltpetre Cave and Stegers Fissure, Augusta County, Virginia. M.S. thesis, Old Dominion Univ., Norfolk, VA 23508. 03 Collins, T.L. and J.R. Holsinger. 1981. Population ecology of the troglobitic isopod crustacean Antrolana lira Bowman (Cirolanidae). Proc. 8th Internat. Cong. Speleol., Bowling Green, KY. 1:129-132. 04 Holsinger, J.R. 1979. Freshwater and terrestrial arthropods - freshwater and terrestrial isopod crustaceans (order Isopoda). Pages 130-148. IN: Proc. of symp. on endangered and threatened plants and animals in Virginia. VA Polytech. Inst. & State Univ., Blacksburg. 05 Holsinger, J.R. 1980. Annotated list of significant caves and karst areas in Virginia. VA Cave Comm. Report. 55 pp. +i-vi. 06 Holsinger, J.R. 1985. Data from research records and files. Dept. of Biol. Sci., Old Dominion Univ., Norfolk, VA 23508. ***** REFERENCES FOR N-OCCURRENCE NARRATIVE ONLY ***** 01 Holsinger, J.R. 1975. Descriptions of Virginia caves. VA Miner. Resour. Bull. 85:1-450. 7 plts. 02 Holsinger, J.R. 1979. Freshwater and terrestrial arthropods - freshwater and terrestrial isopod crustaceans (order Isopoda). Pp. 130-148. IN: Proc. of symp. on endangered and threatened plants and animals in Virginia. VA Polytech. Inst. & State Univ., Blacksburg. 03 Holsinger, J.R. 1980. Annotated list of significant caves and karst areas in Virginia. VA Cave Comm. Rep. 55 pp. +i-vi. 04 Holsinger, J.R. 1985. Data from research records and files. Dept. of Biol. Sci., Old Dominion Univ., Norfolk, VA 23508. 05 Holsinger, J.R. and D.C. Culver. [In Press] The invertebrate cave fauna of Virginia and a part of eastern Tennessee: zoogeography and ecology. Brimleyana, Vol. 14. 06 Collins, T.L. 1982. An ecological study of the troglobitic cirolanid isopod, Antrolana lira Bowman, from Madisons Saltpetre Cave and Stegers Fissure, Augusta County, Virginia. M.S. thesis, Old Dominion Univ., Norfolk, VA 23508. 07 Collins, T.L. and J.R. Holsinger. 1981. Population ecology of the troglobitic isopod crustacean Antrolana lira Bowman (Cirolanidae). Proc. 8th Internat. Cong. Speleology, Bowling Green, KY. 1:129-132. References - 1