(DRAFT) - Taxonomy
                         Species FIDDLENECK, LARGE-FLOWERED
                                Species Id ESIS701032
                                   Date 13 MAR 96



TAXONOMY

NAME - FIDDLENECK, LARGE-FLOWERED OTHER COMMON NAMES - FIDDLENECK, LARGE-FLOWERED; FIDDLENECK, LARGE FLOWERED; FIDDLE-NECK and LARGE-FLOWERED ELEMENT CODE - CATEGORY - Angiosperm PHYLUM AND SUBPHYLUM - MAGNOLIOPHYTA, CLASS AND SUBCLASS - MAGNOLIOPSIDA, ORDER AND SUBORDER - LAMIALES, FAMILY AND SUBFAMILY - BORAGINACEAE, GENUS AND SUBGENUS - AMSINCKIA, SPECIES AND SSP - GRANDIFLORA, SCIENTIFIC NAME - AMSINCKIA GRANDIFLORA AUTHORITY - TAXONOMY REFERENCES - COMMENTS ON TAXONOMY - Large-flowered Fiddleneck Amsinckia grandiflora Kleeb. ex Gray KINGDOM: Plant GROUP: Angiosperm DIVISION: Magnoliophyta CLASS: Magnoliopsida ORDER: Lamiales FAMILY: Boraginaceae Amsinckia gandiflora is an erect colonial annual 3-6 dm tall with bright red-orange flowers borne in a fiddlehead-shaped inflorescence. Leaves and stems are bright (rather than grayish) green and densely covered with coarse, stiff hairs. The leaves are sessile, the lower linear-lanceolate, the upper lanceolate to broadly so, attenuate at apex, and rather densely pustulate. The inflorescence is up to 1-1.5 dm long. The calyx has rust-colored bristles that may completely conceal the appressed hairs beneath the lobes of the calyx fused into 3 or 4, each about 12 mm long. The flowers are 14-18 mm long and are of two morphs, each borne on different plants: one with anthers situated within the corolla tube and the stigma exserted ("pin"); the other with anthers at the mouth of the corolla tube and the stigma located within the tube ("thrum"). Nutlets (seeds) have a smooth, shining surface. Although four nutlets per flower are possible, one, or at the most two nutlets per flower generally develop to maturity in Taxonomy - 1 (DRAFT) - Taxonomy Species FIDDLENECK, LARGE-FLOWERED Species Id ESIS701032 Date 13 MAR 96 this species. Nutlets are plane on the back and sides, the attachment scar broadly lanceolate. The flowering period is March-May (01,02,03,04). This taxon was first described as Amsinckia vernicosa Hook. & Arnott var. grandiflora by Gray in 1876 (01). Another synonym is A. spectabilis Macbr. not Fisch & Mey. (1917) (01). The taxon was given its present treatment as a full species in 1894, but has been cited in recent literature variously as A. grandiflora Kleeb. ex Gray (02,05), Ornduff (07) says this is the correct and most recent treatment. A. grandiflora (Gray) Kleeb. ex Greene (01), and A. grandiflora Kleeb. ex Suksd. (03). Since the early publications, there has been little question of the distinctness of this taxon at the species level. Its unusually large, deep orange, dimorphic flower and smooth nutlets distinguish it from all other California Amsinckia. Several variations of hyphenation and word separation appear, such as large-flowered fiddleneck (the preferred name), large flowered fiddleneck (08), and large-flowered fiddle-neck (03). The type specimen, as reported by Ornduff (01) is Kellogg s. n., no date, CAS? (destroyed), reported from the vicinity of "Antioch, Contra Costa County" (01,03). An excellent drawing of the plant may be found in Abrams vol. 3 (03). Colored slides by Dr. Robert Ornduff are available on request to the California Native Plant Society office in Sacramento, CA (06) or from the photographer himself, in care of the Department of Botany, University of California, Berkeley. Plant specimens are deposited in the following herbaria: Rancho California Academy of Sciences (CAS) in California, other collections may exist outside California. Taxonomy - 2
                                  (DRAFT) - Status
                         Species FIDDLENECK, LARGE-FLOWERED
                                Species Id ESIS701032
                                   Date 13 MAR 96



STATUS

Coded Status E: Federal Endangered COMMENTS ON STATUS - U.S. STATUSES AND LAWS: The large-flowered fiddleneck (Amsinckia grandiflora) has been designated an Endangered species pursuant to the Endangered Species Act of 1973 (50 CFR 17.12; P.L. 93-205, 87 Stat. 884; 16 U.S.C. 1531-1540), as amended. The species has this status wherever found including the State of California. Critical Habitat has been designated in the W 1/2 NW 1/4 and W 1/2 SW 1/4 T3S, R4E San Joaquin County, CA (50 CFR 17.96(a)). Removal and reduction to possession of any federally listed plant from an area under Federal jurisdiction is unlawful (50 CFR 17.61 and 17.71). This species is protected by the Lacey Act (P.L. 97-79, as amended; 16 U.S.C. 3371 et seq.) which makes it unlawful to possess any wild plant (including roots, seeds, and other parts) within U.S. territorial or special maritime jurisdiction (as defined in 18 U.S.C. 7); or to import, export, transport, sell, receive, acquire, or purchase in interstate or foreign commerce any wild plant (including roots, seeds, and other parts) taken, possessed, transported, or sold in violation of any State law or regulation. It is also unlawful to import, export, transport, sell, receive, acquire, or purchase any wild plant (including roots, seeds, and other parts) taken or possessed in violation of any U.S. law, treaty, or regulation or in violation of Indian tribal law. RESPONSIBLE FEDERAL AGENCIES: USFWS -Responsible for the management/recovery, listing, and law enforcement/protection of this species. All Federal agencies have responsibility to ensure that any action authorized, funded, or carried out by that agency is not likely to jeopardize the continued existence of the species or result in the destruction or adverse modification of Critical Habitat (50 CFR 402), and to utilize their authorities to carry out programs for the conservation of the species. STATE STATUSES AND LAWS: STATE: California DESIGNATED STATUS: Endangered ADMINISTRATIVE AGENCY: California Dept. of Fish and Game STATE STATUTE: Calif. Endangered Species Act; Fish and Game Code Chapt. 1.5, Art. 1, Sec. 2050. Status - 1 (DRAFT) - Status Species FIDDLENECK, LARGE-FLOWERED Species Id ESIS701032 Date 13 MAR 96 INTERNATIONAL STATUSES, TREATIES, AND AGREEMENTS: None. ECONOMIC STATUSES: None. 75/07/01:40 FR 27823/27924 - Notice of proposal 76/06/16:41 FR 24523/24572 - Proposed rule 79/12/10:44 FR 70796/70797 - Withdrawal of proposal 80/12/15:45 FR 82480/82569 - Notice of review for proposal 84/05/08:49 FR 19534/19538 - Proposal 85/05/08:50 FR 19374/19378 - Final rule - Endangered w/ Crit. Hab. 85/09/27:50 FR 39526/39527 - Notice of Review Status - 2
     

HABITAT ASSOCIATIONS

HABITAT - TERRESTRIAL TERRESTRIAL LAND USE - Industrial Herbaceous Rangeland COMMENTS ON HABITAT ASSOCIATIONS - The only known occurrence of Amsinckia grandiflora (large- flowered fiddleneck) is on a steeply sloping, west- to northwest-facing hillside (09,14) (Note: Ornduff (01), subsequently cited in USFWS (13) reported the extant site as occurring on south- to southwest-facing slopes. This is in error.) The site is vegetated by herbaceous annuals and perennials consisting of both grasses and broadleaf species. The site is typical of the north-slope phase of the California annual type grassland; that is, it is comparatively densely vegetated, and sustains a relatively high proportion of perennial grasses and broadleaf plant species in comparison with other aspects or exposures of this plant community (14). The substrate required by the species is not well characterized. Ornduff (01) indicates the site is on "light soils"; Rhoads et al. (12) reported that the soils supporting A. grandiflora appeared lighter in color and texture than the heavy-textured, dark gray soils typical of much of Site 300. They report the soils survey map for the area indicates the slope as having soils of the Linne clay loam series, rocky outcrop phase; however, they felt this classification was too general to allow application to that particular hillside where A. grandiflora was found. Some early collections of the species describe its substrate as somewhat alkaline (08). Soil moisture may be relatively high for the California annual type: the known site is on a north slope, and the blue oaks (Quercus douglasii) found nearby indicate that the soil moisture levels are nearly sufficient to support an oak savanna (14). The elevation range for all of the known localities is below 1200 ft (02). Although the known locality for Amsinckia grandiflora is about 10 to 40 percent (09), it is not known that it requires such a steep slope. Since the known locality for A. grandiflora is located within land owned by the Department of Energy, managed by the Lawrence Livermore National Laboratory, and facilities for military testing lie immediately adjacent to the population, both industrial and herbaceous rangeland are indicated as land-type habitat associations. Associated species are Achillea borealis, Silene antirrhina, Stylomecon heterophylla, Trifolium variegatum, Montia perfoliata, Allium amplectens, Poa scabrella, Festuca megalura, Collinsia heterophylla, Melica californica, Lupinus albifrons, L. bicolor, Lithophragma affinis, Marah sp., Phacelia tanacetifolia, Erodium cicutarium, Orthocarpus purpurascens, Dichelostemma pulchella, and Delphinium hesperium (09,14). Avena spp. is most common at the site. Little is known about the adaptations of Amsinckia grandiflora to fire (09). Many, if not most, plants growing in the California annual type grassland are adapted to fire in one way or another, since it is presumed to be a rather frequent event prior to the advent of Europeans in California. In addition, the presence of fire can shift the competitive balance of plants in a grassland, and may influence germination, seedling survival, carbohydrate balance, and reproductive success. However, the season of burning may create dramatically different results in grassland response as well. Research on the Habitat Associations - 1 response of A. grandiflora to fire is needed. The interaction of A. grandiflora and herbivores is not known. Seed predation by birds has been observed in cultivated plants (08), but no incidence of herbivory has been observed in the field (09). The bristly pubescence on the leaves, stems and flowering parts may deter some herbivory. During 1980 field surveys, no incidence of disease was observed on the plants (09). The response of A. grandiflora to weather has not been elucidated, but Cox (17) casually observed that more plants were observed during fairly dry years than during wet years, at least during the 1980's. The relationship between weather and the success of A. grandiflora may indeed be more complex, possibly depending upon average daily temperature, incidence of freezing, and pattern of rainfall throughout the winter season, as well as total precipitation Habitat Associations - 2
                                (DRAFT) - Food Habits
                         Species FIDDLENECK, LARGE-FLOWERED
                                Species Id ESIS701032
                                   Date 13 MAR 96



FOOD HABITS

TROPHIC LEVEL - AUTOTROPH Food Habits - 1
                         (DRAFT) - Environment Associations
                         Species FIDDLENECK, LARGE-FLOWERED
                                Species Id ESIS701032
                                   Date 13 MAR 96



ENVIRONMENTAL ASSOCIATIONS

G = General A = Adult LIM = Limiting RA = Resting Adult J = Juvenile FA = Feeding Adult RJ = Resting Juvenile BA = Breeding Adult FJ = Feeding Juvenile P = Pupae L = Larvae E = Egg RL = Resting Larvae FL = Feeding Larvae
LIFESTAGE ENVIRONMENTAL ASSOCIATIONS G Terrestrial Features: Rock outcrops G Environment Associations - 1
                               (DRAFT) - Life History
                         Species FIDDLENECK, LARGE-FLOWERED
                                Species Id ESIS701032
                                   Date 13 MAR 96



LIFE HISTORY

HABIT: The large-flowered fiddleneck is an herb (01,02,03). LIFE CYCLE: Annual (01,02,03). No information is available on the amount of seed storage in the soil. TYPE OF REPRODUCTION: Sexual (01,04,08,10). Amsinckia grandiflora has two distinct morphs of flowers, each produced on separate plants, which assure out-crossing. The "pin" type flower has an exserted style and enclosed anthers; the "thrum" type flower has a short style and enclosed anthers which are inserted high in the tube and extend to the throat. Pollen grains are dimorphic as well, with each morph corresponding to the flower morph (10). The chief insect visitor is Anthophora edwardsii (10), which preferentially removes thrum pollen. It appears to be a fairly consistent visitor to A. grandiflora, as little foreign pollen was observed on it. Ornduff (10) found the plants had cryptic incompatibility. The number and density of plants has been observed to fluctuate greatly from year to year. In most years, the ratio of pin to thrum bearing plants is about equal, but in years in which few plants appear, the ratio may be considerably different (10). REPRODUCTIVE PHENOLOGY: The germination and leafing dates are not known. Flowering most likely begins in early March and continues through anthesis (01,14), which occurs from mid-March to mid-May (17). Fruiting begins soon after first anthesis. Seed/fruit dispersal dates are not known. Amsinckia grandiflora has been grown on many occasions by Dr. Robert Ornduff and others at the University of California Botanical Garden (07). Some unpublished information is undoubtedly available through this source, although Dr. Ornduff indicates the behavior of the cultivated plants may not exactly replicate what takes place in the field. SEX OR SPORE STATUS: Monoecious (10,19). A. grandiflora is monoecious, but distylous; only one morph is present on each plant. Each morph has its own size and type of pollen (10). Based on field observations by Ornduff (10), the pin stigmas receive more pollen grains than thrum stigmas, and also a greater proportion of illegitimate pollen than thrums. Seasonal fluctuations in the representation of the two morphs have no striking influence on pollen-flow patterns. The pin:thrum ratio has been observed to be 1:1 on some years, and 2:1 in some seasons. The plants appear to possess cryptic incompatibility (10). POLLINATION, SPORE AND SEED DISSEMINATION: Pollen dissemination is done by insects, primarily Anthophora edwardsii (07,10). Ornduff (10) reported preferential collection of thrum flower pollen by most flower visitors; this may be due to the Life History - 1 (DRAFT) - Life History Species FIDDLENECK, LARGE-FLOWERED Species Id ESIS701032 Date 13 MAR 96 easier accessibility of pollen produced by the thrum anthers, since these are situated at the throat of the corolla lobe. Foreign pollen was generally present in small quantities, suggesting that the pollinators are rather specific to this species of plant. No information available on seed dissemination, although Ornduff (07) has observed birds eating the seeds. SEED BIOLOGY: Seed production by A. grandiflora ranges from slightly less than 1.0 to about 1.5 seeds per flower, considerably less than the possible maximum of four nutlets often produced by more seedy members of the genus. No data appeared available in the literature on seed production per plant. However, this plant is an indeterminate flowering species, so seed production would be expected to vary considerably depending on how favorable conditions are. Ornduff (07) felt that there might not be any dormancy requirement, although seeds have been found to be viable over many years in cold storage. Dr. Herbert Baker pointed out (18) that since the species is an annual, and often a poor growth and seed production year may be followed by a year of high germination and growth, seed storage is undoubtedly an important mechanism for the species to avail itself of favorable conditions. No additional information is available on this, however. Cultivation of several Amsinckia by Ray and Chisaki (04) indicated that seeds could easily be germinated in moist sand; however seed sown up to a few months after harvest would germinate only if first stratified by placing in moist filter paper at 4 deg. C for 1-4 weeks. Seedlings raised in the spring produced larger and more vigorous plants than those raised in summer. POPULATION BIOLOGY: Since population numbers have been less than 200 per year between 1980 - 1986 (08,14,17), A. grandiflora may be experiencing some disruptions in pin:thrum ratios and possibly other less obvious deleterious effects of genetic bottleneck. Numbers have been slightly higher in 1984 - 1986 than in the previous four; this may be due to a variety of factors, weather conditions being among the most important. Little is known about survival and mortality rates, except some unpublished observations by Ornduff (07) concerning plants grown in the Univ. of Calif. Berkeley Botanic Garden. He found the plant easy to grow in cultivation, with high germination and good seed set. He could provide details on the cultivation of the plant, but has not closely observed the entire cycle in the wild. Population trends: there appears to be a definite decline in population numbers since the 1950's and 1960's, when the plant was described as "abundant", and "in the thousands" of individuals (08). As indicated in previous sections, the cause of the decline is not known with certainty, and may be due to a combination of factors. Causes of mortality: seedling mortality rates are not known, but plants reaching anthesis have been observed to be free of herbivory and disease (12,09). Since the plant is an annual, mortality rates are not as meaningful for longer-lived plants. Recovery potential: much more information is needed about the Life History - 2 (DRAFT) - Life History Species FIDDLENECK, LARGE-FLOWERED Species Id ESIS701032 Date 13 MAR 96 biology of the plant in order to determine the feasibility of active intervention for this species. It has been suggested that its primitive and relatively inefficient reproductive system has caused it to be out-competed by more weedy Amsinckia, and this species simply represents the end of an evolutionary line. However, if the suggestions that man-caused factors are contributing significantly to the decline in the species, it is desirable to take steps to favor the species as much as possible. ECOLOGICAL/EDAPHIC FACTORS: The only known occurrence of Amsinckia grandiflora occurs in an area mapped by the Soil Conservation Service as Linne clay loams. However, collections records and present-day observations indicate that the soils at the actual site of the population is lighter in color and texture (01) than the surrounding soils and therefore may represent a somewhat different condition. Some collectors have indicated that the soils where the plant occurs are somewhat alkaline (20). A. grandiflora now occurs only on a steep, west- and northwest- facing slope ranging from 10 to 40 degrees. The site where the population occurs appears to be relatively undisturbed, in spite of Drop Tower 858 being located within a meter of the population (14). TROPHIC STATUS: Phototrophic (01,09,12). Amsinckia grandiflora is a phototrophic species. It is not known to have any parasitic or semi-parasitic relations with any other plants. It is not known to host any symbiotic species, or to have any noticeable diseases (09,12). CHARACTERISTIC DOMINANCE: Component (01). Although A. grandiflora is a showy plant with colonies visible from some distance, it has probably never been a dominant species, even within the small areas in which colonies grow. At best, it can be described as a component of the grassland, but never a co-dominant species. COMMUNITY ECOLOGY: All known collections of A. grandiflora have been described as growing in grassland which would today be called the California annual grassland (20). It is likely that in pristine times, the dominant grasses would have been perennials such as Stipa and Poa. These species persist at the known locality of A. grandiflora, but Bromus diandrus and Avena spp. are the most abundant species (14). Much has been written about the dynamics of the California annual grassland. Fire, weather, and grazing affect the competitive relationships between species; the response of the vegetation is rapid because of the high proportion of annual species present. The relationship of A. grandiflora to other grassland species is not well understood. SPECIES INTERRELATIONSHIPS: No information was found in the literature on known herbivores or disease-causing organisms. Field observers have noted the absence of Life History - 3 (DRAFT) - Life History Species FIDDLENECK, LARGE-FLOWERED Species Id ESIS701032 Date 13 MAR 96 disease and evidence of herbivory on plants in the field (09). The bee Anthophora edwardsii Cresson (Anthophoridae) was observed to be the primary insect pollinator of Amsinckia grandiflora (10); it appeared to collect pollen from the pin morph, presumably because the thrum anthers are situated in a more accessible location at the throat of the corolla. OTHER LIFE HISTORY DESCRIPTORS: None. Life History - 4
                           (DRAFT) - Management Practices
                         Species FIDDLENECK, LARGE-FLOWERED
                                Species Id ESIS701032
                                   Date 13 MAR 96



MANAGEMENT PRACTICES

RESULT MANAGEMENT PRACTICE Beneficial Suppressing wildfire Beneficial Prescribed/controlled burning of habitat Beneficial Restricting/regulating human disturbance of populations Beneficial Land Acquisition Beneficial Controlling/Removing Nonnative Vegetation Beneficial Stocking captive-reared wild-strain animals Beneficial Controlling/Removing Invertebrates Beneficial Controlling/Removing Native Vertebrates Adverse Artillery/Explosions Existing Artillery/Explosions Adverse Inherent Reproductive Characteristics Existing Inherent Reproductive Characteristics Adverse Low Gene Pool Existing Low Gene Pool Adverse Rural Residential/Industrial Areas Existing Rural Residential/Industrial Areas Adverse Highway/Railroads Existing Highway/Railroads Adverse Soil compaction by heavy equipment in mine areas Existing Soil compaction by heavy equipment in mine areas Adverse Strip mining Existing Strip mining Adverse Competition Existing Competition Adverse Exotic/Feral/Introducted Species Existing Exotic/Feral/Introducted Species Adverse Grazing Existing Grazing Adverse Existing Adverse Vegetation Composition Changes Existing Vegetation Composition Changes Adverse Suppressing wildfire Existing Suppressing wildfire Adverse Fire Existing Fire COMMENTS ON MANAGEMENT PRACTICES - Historical records suggest that Amsinckia grandiflora has always been a rare plant, and that it has become rarer in this century (09). Plant numbers have declined from several thousand plants in 1966 (10) to less than 200 in most subsequent years for which data are available (08). No one is certain why the plant has declined, although many reasons have been forwarded, and several factors in combination may have contributed to its present diminished condition. Human land use: The areas near the collection sites for A. grandiflora have been much altered since the first collections were made. The area near Antioch has been used for ranching, agriculture, Management Practices - 1 (DRAFT) - Management Practices Species FIDDLENECK, LARGE-FLOWERED Species Id ESIS701032 Date 13 MAR 96 and, more recently, housing. Since the exact location of the earliest collections is not known, the fate of those sites cannot be ascertained. Most of the Livermore Valley, another collection site, has been used for ranching for many years, and in the past decade has become more suburban. Some man-caused influences may affect the extant site in particular. Construction of a paved roadway above the population at Site 300 may influence runoff patterns (01), and the placement of spoils when the Drop Tower 858 was constructed in 1958 may have diminished the available suitable habitat for the species (11). Rhoads and Sauls (12) noted some heavy concrete blocks in the arroyo below the drop tower and some erosion below a drain pipe east of the existing population; however, these did not appear to be influencing the extant colony. The documentation for Federal listing of A. grandiflora (13) suggested that chemical explosive testing on Site 300 may affect at the species, or that accidental fires caused by the testing at the Site 300 may have a detrimental effect on the population. It is not clear that this connection has been established between ongoing activities by the Department of Energy and the survival of A. grandiflora; however, testing activities take place very near the population and precautionary measures to protect the species are desired by the U.S. Fish and Wildlife Service. Normal responses to variations in habitat: The single extant population varies considerably in size from year to year, as do many species of annual plants found in the valley grasslands of California. The exact reasons for these variations in Amsinckia grandiflora are not known (01). Many California annual grassland species respond strongly to combinations and patterns of precipitation and temperature which may exert a double influence: directly, plant germination and seedling survival may be influenced by weather patterns; indirectly, germination and seedling survival of competing species may limit available habitat for the plant. Grazing: Several authors have suggested that the introduction of exotic grazing animals may have contributed to the reduction of this species throughout its range (01). Others have suggested that competition with non-native grassland plants may be a contributing factor (13). Indeed, the one remaining site persists on a site ungrazed by domestic livestock for over 30 years (09). Competition: Because Amsinckia grandiflora grows in a relatively high-competition site its poor success may, indeed, be indicative of a poor competitor with non-native introduced grasses and herbs, as are many native species. Moreover, several authors have noted the striking contrast between A. grandiflora, A. lunaris, and A. furcata, all rather low-competition members of the genus, and the widespread, abundant, and rather weedy A. intermedia and A. tessellata (10,15). Management factors: The absence of grassland fires or the occurrence of fires at unnatural seasons may also affect the ability of Amsinckia grandiflora to germinate, become established, or compete effectively against other grassland species (13,14). Little experimental evidence is available on this point. Although Lawrence Livermore National Laboratory does carry out preventive controlled burning, no such burns have been conducted in the immediately vicinity of the A. grandflora site. Accidental fires are a possibility due to Management Practices - 2 (DRAFT) - Management Practices Species FIDDLENECK, LARGE-FLOWERED Species Id ESIS701032 Date 13 MAR 96 testing activities at Site 300 and might take place at a time of year detrimental to the species. However, controlled burns elsewhere on the site which have benefitted many of the native grassland species has not improved conditions for A. grandiflora. Reproductive conditions: Amsinckia grandiflora has a highly specialized pollination system that ensures out-crossing, but may result in lowered seed production, especially when total population numbers are low (13). In addition, reproduction may be disrupted by imbalances in the equilibrium of the flower morphs, a factor which becomes more significant when population levels are low (10). UNAPPROVED PLAN: U.S. Fish and Wildlife Service. 1986. Recovery Plan for the Large- flowered Fiddleneck (Amsinckia grandiflora) Agency Draft. U.S. Fish and Wildlife Service, Portland, OR. 52 pp. The primary objective for the recovery plan of the large-flowered fiddleneck is secure and augment the single, known population on Department of Energy land to meet certain criteria for declassifying to threatened status and delisting the species. The criteria for declassifying is to establish two more secure populations which contain at least 2500 plants and are self-sustaining for five years; and for delisting, four secure, self-sustaining populations with at least 2500 plants each, must be established for a 10 year period. The recovery activities necessary for accomplishing this objective are: 1) protect the species and habitat from physical disturbance by securing a long-term cooperative agreement with the DOE and Lawrence Livermore Labs. Although human access is controlled at the site due to security the staff and maintenance impacts and activities must be controlled. 2) enhance the present population and its habitat by implementing exotic plant and predator/herbivore (bird/insect) control measures and by managing habitat to reduce competition, maintain plant vigor, and promote natural regeneration; 3) establish additional populations by propagation with collected seed, out-planting in suitable historic habitat, establishing conservation agreements, and monitoring of established populations; 4) implement management/recovery actions (based on research results) which identify limiting factors for fruit, seed, seedling recruitment, identify potential habitat, and determine requirements for maintaining habitats: Habitat management actions could include prescribed burning, hand weeding, or chemical means as determined by research; Fire control at certain times of the year may be necessary since season of burning may be significant; 5) locate and protect additional fiddleneck populations; and 6) enforce protective laws and regulations. Management Practices - 3
                                   (DRAFT) - References
                            Species FIDDLENECK, LARGE-FLOWERED
                                  Species Id ESIS701032
                                      Date 13 MAR 96



     

References

***** REFERENCES FOR ALL NARRATIVES EXCEPT N-OCCURRENCE ***** 01 Ornduff, R. 1977. Rare plant status report, Amsinckia grandiflora. Calif. Nat. Plant Soc., Sacramento. 3 pp. 02 Munz, P. and D. Keck. 1973. A California flora and supplement. Univ. of CA Press, Berkeley. 1905 pp. 03 Abrams, L. 1967. An illustrated flora of the Pacific states, Vol. 3. Stanford Univ. Press, Stanford, CA. 806 pp. 04 Ray, P.M. and H.F. Chisaki. 1957. Studies on Amsinckia. I. A. synopsis of the genus, with a study of heterostyly in it. Am. J. Bot. 44:529-535. 05 Kartesz, J.T. and R. Kartesz. 1980. A synonymized checklist of the vascular flora of the United States, Canada, and Greenland. Vol. 2., the biota of North Am. 498 pp. 06 California Native Plant Society. Dr. Ornduff color slides. 909 12th St., Sacramento, CA 95814. 07 Ornduff, R. 1986. Pers. comm. Botany Dept., Univ. of CA, Berkeley. 94720. 08 U.S. Fish and Wildlife Service. 1986. Draft recovery plan, Amsinckia grandiflora. USFWS, Sacramento, CA 48 pp. 09 Rhoads, W.A., T.P. O'Farrell, and M.L. Sauls. 1981. Occurrence and status of endangered species, San Joaquin kit fox, Vulpes macrotis mutica, and large-flowered fiddleneck, Amsinckia grandiflora on Lawrence Livermore National Laboratory, Site 300, CA. EG&G Co., Santa Barbara, CA. 21 pp. 10 Ornduff, R. 1976. The reproductive system of Amsinckia grandiflora, a distylous species. Systematic Botany 1(1):57-66. 11 York, R.P. 1986. Personal comm. Calif. Nat. Plant Soc., Sacramento, CA. 12 Rhoads, W.A. and M.L. Sauls. 1980. Preliminary report, an investigation for the occurrence and status of threatened or endangered species on Lawrence Livermore Lab. Site 300. EG&G Co., Santa Barbara, CA. 9 pp. 13 U.S. Fish and Wildlife Service. 1985. Determination that Amsinckia grandiflora is an endangered species and designation of critical habitat. Fed. Reg. 50: 19374-19378. 14 Leitner, B.M. 1986. Pers. obs. St. Mary's College, P.O. Box 163, Moraga, CA. 15 Ganders, F.R. 1975. Heterostyly, homostyly, and fecundity in Amsinckia spectabilis (Boraginaceae) Madrono 23:56-62. 16 Weller, S.G. and R. Ornduff. 1977. Cryptic self-incompatibility in Amsinckia grandiflora. Evol. 31:47-51. 17 Cox, Robin. 1984. Element preservation plan, Amsinckia grandiflora. The Nature Conservancy, San Francisco, CA. 3 pp. 18 Baker, H. 1986. Pers. comm. Univ. of CA, Berkeley. 19 Ray, P. and H.F. Chisaki. 1957. Studies on Amsinckia. I. A synopsis of the genus, with a study of heterostyly in it. Am. J. Bot. 44:524-536. 20 Department of Fish and Game. 1986. Element occurrence records, Amsinckia grandiflora. California Natural Diversity (Data Base). Sacramento, CA. References - 1 (DRAFT) - References Species FIDDLENECK, LARGE-FLOWERED Species Id ESIS701032 Date 13 MAR 96 ***** REFERENCES FOR N-OCCURRENCE NARRATIVE ONLY ***** 01 California Fish and Game. 1986. Element occurrence records, Amsinckia grandiflora. California Natural Diversity (Data Base). Sacramento, CA. 02 Abrams, Leroy. 1967. An illustrated flora of the Pacific states, Vol 3. Stanford Univ. Press, Stanford, CA. 806 pp. 03 Rhoads, W.A. and Mary L. Sauls. 1980. Preliminary report, an investigation for the occurrence and status of threatened or endangered species on Lawrence Livermore Laboratory Site 300. EG&G Co., Santa Barbara, CA. 9 pp. 04 Ornduff, R. 1977. Rare plant status report, Amsinckia grandiflora Calif. Nat. Plant Soc., Sacramento. 3 pp. 05 York, Rick P. Pers. comm. Botanist, Cal. Native Plant Society, Sacramento, CA. 3 pp. References - 2