(DRAFT) - Taxonomy
                             Species AMPHIANTHUS, LITTLE
                                Species Id ESIS704051
                                   Date 13 MAR 96



TAXONOMY

NAME - AMPHIANTHUS, LITTLE OTHER COMMON NAMES - AMPHIANTHUS, LITTLE;SPRITE, POOL; AMPHIANTHUS and DIMINUTIVE ELEMENT CODE - CATEGORY - Angiosperm PHYLUM AND SUBPHYLUM - MAGNOLIOPHYTA, CLASS AND SUBCLASS - MAGNOLIOPSIDA, ORDER AND SUBORDER - SCROPHULARIALES, FAMILY AND SUBFAMILY - SCROPHULARIACEAE, GENUS AND SUBGENUS - AMPHIANTHUS, SPECIES AND SSP - PUSILLUS, SCIENTIFIC NAME - AMPHIANTHUS PUSILLUS AUTHORITY - TAXONOMY REFERENCES - COMMENTS ON TAXONOMY - Little Amphianthus Amphianthus pusillus Torrey KINGDOM: Plant GROUP: Angiosperm DIVISION: Magnoliophyta CLASS: Magnoliopsida ORDER: Scrophulariales FAMILY: Scrophulariaceae Amphianthus pusillus is a diminutive fibrous-rooted annual. It has both floating and submerged leaves. The submerged leaves are lanceolate, less than 1 centimeter (cm) (0.4 inches) in length and appear to be arranged in a basal rosette. The floating leaves are ovate, 4-8 millimeters (mm) (0.16-0.342 inches) long, 3-5 mm (0.12-0.2 inches) wide, opositely arranged, and attached to the stem near the submerged leaves by long, delicate stems. Its flowers are white, 4-5 mm (0.16-0.2 inches) in length, and are borne in the axils of both types of leaves. Floating flowers are chasmogamous (open) and submerged flowers are cleistogamous (closed) except when exposed to air (09,16). This species was first collected by M.C. Leavenworth in 1836 in Newton County, Georgia (present-day Rockdale County) and later described by John Torrey in 1839 (14). Amphianthus pusillus is thought to be a relict species, representing a monotypic genus of Taxonomy - 1 (DRAFT) - Taxonomy Species AMPHIANTHUS, LITTLE Species Id ESIS704051 Date 13 MAR 96 doubtful placement in the family Scrophulariaceae (10,13,14). It is most similar in flower morphology to Gratiola and Bacopa but differs from all other southeastern Scrophulariaceae by its dimorphic leaves and flowers (06,14). Amphianthus produces a capsule, 2-3 mm (0.08- 0.12 inches) broad and 1 mm (0.04 inch) long. To date no controversial taxonomic problems exist. The holotype is located in the New York Botanical Garden Herbarium. Descriptions and/or illustrations of Amphianthus can be found in Bridges (03), Kral (06), Lunsford (09), Pennell (14), and Rayner (16). Other common names used in literature are: pool sprite, and diminutive amphianthus. Taxonomy - 2
                                  (DRAFT) - Status
                             Species AMPHIANTHUS, LITTLE
                                Species Id ESIS704051
                                   Date 13 MAR 96



STATUS

Coded Status Alabama; Federal Threatened Alabama; Unofficially Listed Georgia; Federal Threatened Georgia; State Listed South Carolina; Unofficially Listed T: Federal Threatened COMMENTS ON STATUS - U.S. STATUSES AND LAWS: The little amphianthus (Amphianthus pusillus) has been designated a threatened species pursuant to the Endangered Species Act of 1973 (50 CFR 17.12; P.L. 93-205, 87 Stat. 884; 16 U.S.C. 1531-1540), as amended. The species has this status wherever found including the States of Alabama, Georgia, and South Carolina. This species is protected by the Lacey Act (P.L. 97-79, as amended; 16 U.S.C. 3371 et seq.) which makes it unlawful to possess any wild plant (including roots, seeds, and other parts) within U.S. territorial or special maritime jurisdiction (as defined in 18 U.S.C. 7); or to import, export, transport, sell, receive, acquire, or purchase in interstate or foreign commerce any wild plant (including roots, seeds, and other parts) taken, possessed, transported, or sold in violation of any State law or regulation. It is also unlawful to import, export, transport, sell, receive, acquire, or purchase any wild plant (including roots, seeds, and other parts) taken or possessed in violation of any U.S. law, treaty, or regulation or in violation of Indian tribal law. RESPONSIBLE FEDERAL AGENCIES: USFWS -Responsible for the management/recovery, listing, and law enforcement/protection of this species. All Federal agencies have responsibility to ensure that any action authorized, funded, or carried out by that agency is not likely to jeopardize the continued existence of the species or result in the destruction or adverse modification of Critical Habitat (50 CFR 402), and to utilize their authorities to carry out programs for the conservation of the species. STATE STATUSES AND LAWS: STATE: Georgia DESIGNATED STATUS: Endangered ADMINISTRATIVE AGENCY: Georgia Dept. of Natural Resources STATE STATUTE: Wildflower Presrevation Act of 1973 STATE: Alabama, South Carolina Status - 1 (DRAFT) - Status Species AMPHIANTHUS, LITTLE Species Id ESIS704051 Date 13 MAR 96 UNOFFICIAL LIST: AL - Endangered, Vascular Plant Species Critical to Maintenance of Floristic Diversity in Alabama SC - National Concern-Endangered, Native Vascular Plants Rare, Threatened or Endangered in South Carolina INTERNATIONAL STATUSES, TREATIES, AND AGREEMENTS: None. ECONOMIC STATUSES: None. 75/08/01:40 FR 27823/ - Smithsonian rule 76/06/16:41 FR 24523/ - Proposed rule 80/12/15:45 FR 82479/ - Notice of review 85/09/27:50 FR 39526/ - Notice of review 87/02/09:52 FR 05150/ - Proposed rule 88/02/05:53 FR 03560/03565 - Final rule - Endangered Status - 2
     

HABITAT ASSOCIATIONS

HABITAT - AQUATIC TERRESTRIAL TERRESTRIAL LAND USE - Bare Exposed Rock Strip Mines, Quarries, and Gravel Pits NATIONAL WETLAND INVENTORY CODES NWI NWICLS NWIMOD NWISPEC Palustrine EM COMMENTS ON HABITAT ASSOCIATIONS - Amphianthus pusillus (little amphianthus) typically occurs in shallow flat-bottomed pools found on the crest and flattened slopes of porphyritic granite outcrops (05,08,16). Such pools have been referred to as vernal pools (06,16), weather pits (01), depression pits (13) and solution pits (08,10). Few species occur directly with Amphianthus pusillus due to the aquatic nature of the microhabitat (10). Isoetes melanospora and I. tegetiformans are the most common associates. Other plants which occur in and around the pools include lichens (Cladonia sp.), Diamorpha smallii, Arenaria uniflora, A. glabra, Polytrichum commune, Isoetes piedmontana, Juncas georgians, Agrostis sp., Lindernia monticola, Cypress granitophilus, Selaginella tortipila and Andropogon scoparius (05,06,16). Surrounding vegetation where a sufficient soil depth exists is oak-yellow pine forest (06). Outcrops supporting Amphianthus pusillus populations range in topography from dom-shaped to flat. Pools containing Amphianthus pusillus are several meters in diameter and are circular or irregularly shaped due to the coalescence of adjacent pools (09,10). They are surrounded by a rock rim several centimeters in height and retain water for several weeks following heavy rains and completely dry out with summer droughts (05,09,10,12,16). Most populations occur in such typical pools; however, Garris (05) and Rayner (16) have reported several populations from atypical habits. Most of these atypical pools lacked an intact rim, others were in ecotonal zones or seepage areas. Soils in these pools are formed from sandy-silty wash which are 2-5 cm (0.8-2.0 inches) in depth (16)., low in organic matter (0.3-0.6 percent), nitrogen, phosphorous, potassium and mineral salts (07,09); a subsoil is lacking (08). Amphianthus pusillus has adapted to the extreme environmental conditions on granite outcrops including high light intensities, extreme temperatures and wet/dry periods (07,10). The water depth in the pools ranges from 0.5 cm to 10 cm (0.2 - 4 inches) (02,03). Habitat Associations - 1
                                (DRAFT) - Food Habits
                             Species AMPHIANTHUS, LITTLE
                                Species Id ESIS704051
                                   Date 13 MAR 96



FOOD HABITS

TROPHIC LEVEL - AUTOTROPH Food Habits - 1
                         (DRAFT) - Environment Associations
                             Species AMPHIANTHUS, LITTLE
                                Species Id ESIS704051
                                   Date 13 MAR 96



ENVIRONMENTAL ASSOCIATIONS

G = General A = Adult LIM = Limiting RA = Resting Adult J = Juvenile FA = Feeding Adult RJ = Resting Juvenile BA = Breeding Adult FJ = Feeding Juvenile P = Pupae L = Larvae E = Egg RL = Resting Larvae FL = Feeding Larvae
LIFESTAGE ENVIRONMENTAL ASSOCIATIONS G G Terrestrial Features: Rock outcrops G G Environment Associations - 1
                               (DRAFT) - Life History
                             Species AMPHIANTHUS, LITTLE
                                Species Id ESIS704051
                                   Date 13 MAR 96



LIFE HISTORY

HABIT: Amphianthus pusillus (little amphianthus) is an emergent (17). LIFE CYCLE: Amphianthus is ephemeral, usually completing its life cycle in a 3 to 4 week period (05,06); however, individual plants can probably live a maximum of three months (16). Amphianthus is a winter annual (09). TYPE OF REPRODUCTION: There are two types of flowers: floating flowers are chasmogamous (open) and cross pollinated; submerged flowers are cleistogamous (closed) and self-pollinated. Cleistogamous flowers will open when exposed to air (09,16). Preliminary research by Randell (15) suggests that the principle mode of reproduction is agamospermy. REPRODUCTIVE PHENOLOGY: Leafing occurs in December to January, budding in February, anthesis in mid-late February to April, fruiting in April-May, and seed dispersal in May-June (03,05,09,11,16). Germination seems to be dependent only upon presence of light and of sufficient standing water or soil saturation in its habitat (03). According to Lunsford (09) germination occurs within 3 to 4 days following establishment of suitable conditions (sufficient rainfall) and an unknown dormancy period. Amphianthus will germinate and flower after heavy summer rains (09). SEX OR SPORE STATUS: Monoclinous (09). POLLINATION, SPORE AND SEED DISSEMINATION: Pollem dissemination agents are unknown (03,09,16). Seed dispersal by outwash from heavy rains may be a short-distance dispersal mechanism. Other dispersal agents may be by animal use of the pools (i.e., seeds or whole plants may adhere to their feet and be transported to suitable habitat (03,09,16). SEED BIOLOGY: Germination of Amphianthus seems to be dependent only on presence of light and sufficient standing water or soil saturation in its habitat (03). Exact dormancy period of seeds is unknown, however, it appears seeds can remain dormant for several years (09,14,16). Capsules contain numerous seeds (16). POPULATION BIOLOGY: Population levels fluctuate depending upon local weather conditions (sufficient moisture). Drought-induced mortality is the most important, of natural cause of mortality (16). This occurs when adequate rainfall induces seed germination but rainfall thereafter is insufficient to allow completion of development. Amphianthus is not a vigorous competitor and could be eliminated by overcrowding and Life History - 1 (DRAFT) - Life History Species AMPHIANTHUS, LITTLE Species Id ESIS704051 Date 13 MAR 96 Life History - 2 (DRAFT) - Life History Species AMPHIANTHUS, LITTLE Species Id ESIS704051 Date 13 MAR 96 shading (06,07,16). Seed production and seed dormancy under normal conditions are sufficient to maintain existing populations (16). Recovery potential is less related to poulation biology than preventing habitat destruction or modification. ECOLOGICAL/EDAPHIC FACTORS: Outcrops supporting Amphianthus pusillus populations range in topography from dom-shaped to flat. These outcrops are located in the Piedmont physiographic region, and contain materials of precambrian metamorphic rock and differ geologically as igneous, quartzitic, gneissic or porphyritic granite (08,10). They are ordinarily found on uplands between river valleys (10). Pools containing Amphianthus pusillus are several meters in diameter and are circular or irregularly shaped due to the coalescence of adjacent pools (09,10). They are surrounded by a rock rim several centimeters in height and retain water for several weeks following heavy rains and completely dry out with summer droughts (05,09,10,12,16). Most populations occur in such typical pools; however, Garris (05) and Rayner (16) have reported several populations from atypical habits. Most of these atypical pools lacked an intact rim, others were in ecotonal zones or seepage areas. Soils in these pools are formed from sandy-silty wash which are 2-5 cm (0.8-2.0 inches) in depth (some pools have greater soil depths) (16)., low in organic matter (0.3-0.6 percent), nitrogen, phosphorous, potassium and mineral salts (07,09); a subsoil is lacking (08). Amphianthus pusillus has adapted to the extreme environmental conditions on granite outcrops including high light intensities, extreme temperatures and wet/dry periods (07,10). The water depth in the pools ranges from 0.5 cm to 10 cm (0.2 - 4 inches) (02,03). TROPHIC STATUS: Phototrophic. CHARACTERISTIC DOMINANCE: Dominant/codominant (09,16). Abundance of Amphianthus varies with local weather conditions. It is an annual which is abundant when suitable environmental conditions exists (sufficient moisture); however, it occurs in low numbers, if at all, during drought (09,16). COMMUNITY ECOLOGY: Amphianthus is one of the first species to establish innewly formed soil in poolson granite outcrops (09). This species lives in areas of high light intensities with soils of low salt content which precludes succession for a time (07). However, this species is forced out as soil depth increases and water holding capacity increases allowing more competitive species to establish. However, this process is slow and new habitat is constantly being created (04). SPECIES INTERRELATIONSHIPS: None known. OTHER LIFE HISTORY DESCRIPTORS: None. Life History - 3 (DRAFT) - Life History Species AMPHIANTHUS, LITTLE Species Id ESIS704051 Date 13 MAR 96 Life History - 4
                           (DRAFT) - Management Practices
                             Species AMPHIANTHUS, LITTLE
                                Species Id ESIS704051
                                   Date 13 MAR 96



MANAGEMENT PRACTICES

RESULT MANAGEMENT PRACTICE Beneficial Controlling/Restricting Off-Road Vehicles Beneficial Restricting/regulating human disturbance of populations Beneficial Controlling/Restricting Mining Beneficial Land Acquisition Beneficial Controlling/Removing Domestic Animals Adverse Harassment/Vandalism/Indiscriminate Killing Existing Harassment/Vandalism/Indiscriminate Killing Adverse Off Road Vehicles Existing Off Road Vehicles Adverse Hiking/Camping Existing Hiking/Camping Adverse Inherent Reproductive Characteristics Existing Inherent Reproductive Characteristics Adverse Low Gene Pool Existing Low Gene Pool Adverse Surface Mines Existing Surface Mines Adverse Recreational development Existing Recreational development Adverse Competition Existing Competition Adverse Grazing Existing Grazing COMMENTS ON MANAGEMENT PRACTICES - Amphianthus is restricted to granite outcrops in the Piedmont physiographic region. The major threat to this species is the destruction and adverse modification of its habitat. Populations have been lost through quarrying and the fate of several extant populations is tenuous since several areas are active quarry sites (17). Granit outcrops are popular recreation sites and unfortunately such attention and overuse have resulted in damage to the geologic structures and vegetation (05). Many of the pools supporting populations have been directly damaged by vehicular traffic. Vehicular traffic during this species' growing season poses a seriuos threat by uprooting/crushing live plants, hastening the erosion of the pools' rims and displacing soil from the pools (02,16). ORV's have decreased the vigor of all of the South Carolina Amphinathus populations (16) and destroyed one Alabama population (12). Pools have been further impacted by such activities as fire building and littering (05,16). Rearrangement of stones in two pools has caused a decline in two populations of Amphianthus. Granite outcrops are often enclosed in pasture. A concentration of grazing animals on these areas has caused damage to vernal pool vegetation through trampling and has added nutrients to the water, which favors the growth of more competitive aquatics (03,05). Such eutrophication of vernal pools has eliminated Amphianthus from Management Practices - 1 (DRAFT) - Management Practices Species AMPHIANTHUS, LITTLE Species Id ESIS704051 Date 13 MAR 96 several pools at one site and caused the decline of Amphianthus at a second area. Many of the smaller outcrops are used as local dumps or for storing equipment, and such land use has destroyed two populations of Amphianthus in Georgia (05,17). Amphianthus is vulnerable due to its requirements for special environmental conditions (moisture,light) for germination and growth and an unknown dormancy period for the seeds (05,09,16). One factor beleived to contribute to the rarity of Amphianthus is the lack of adaptation for seed dispersal (09). Preliminary research by Randall (15) suggests that the principal mode of reproduction is agamospermy (production of seeds by asexual means) and this asexual reproduction threatens its adaptive potential. Flatrocks in the Southeast are being considered as possible repositories for nuclear waste, and this poses a potential future threat to Amphianthus and its habitat (16). Many of the populations consists of small numbers of individuals confined to only one ot two pools so local extinction through natural causes is possible. UNAPPROVED PLAN: No recovery plan has been developed for little amphianthus (Amphianthus pusillus), but one should be initiated by FY90. There is no approved recovery plan for Amphianthus pusillus at the present time. Actions recommended for recovery of this species include: 1. Inventory and determine the size and stability of populations. 2. Protect habitat and existing populations through land acquisition and the use of Cooperative Agreements. Priority sites are those supporting large populations and those at the periphery of the range. 3. Eliminate grazing of domestic animals near pools with populations by fencing that portion of the outcrop. 4. Prohibit vehicular traffic on outcrops by blocking access points with gates or boulders. 5. Monitor recreational use of protected outcrops and limit to small or well-supervised groups. 6. Educate quarry company personnel about populations in active quarries and restrict those areas from quarrying activity. 7. Monitor and collect baseline data on existing populations (population size, associated species, microhabitat), emphasizing research on poulation fluctuations as related to weather factors and disturbance. 8. Conduct research on reproductive biology and autecology. Management Practices - 2
                                   (DRAFT) - References
                               Species AMPHIANTHUS, LITTLE
                                  Species Id ESIS704051
                                      Date 13 MAR 96



     

References

***** REFERENCES FOR ALL NARRATIVES EXCEPT N-OCCURRENCE ***** 01 Bake, W.A. Jr. 1970. Georgia granite. Nat. Hist. 79:32-39. 02 Bridges, E.L. 1986. Population inventory and mapping and the establishment of a monitoring system for rare plant species at Heggie's Rock Perserve, Columbia County, Georgia. Unpubl. rpt. to: Nature Conservancy, Chapel Hill, NC. 62 pp. 03 Bridges, F.L. 1986. Stewardship abstract for Amphianthus pusillus. Prepared for: The Nature Conservancy, Chapel Hill, NC. 7 pp. 04 Burbanck, M.P., and R.B. Platt. 1964. Granite outcrop communities of the Piedmont Plateau in Georgia. Ecology 45:292-306. 05 Garris, R.S. 1980. The endangered endemic, Amphianthus pusillus: a study of distribution and density. GA Dept. of Nat. Res., Protected Pllants/Natural Areas Program. Unpubl. rpt. 20 pp. 06 Kral, R. 1983. A report on some rare, threatened, or endangered forest-related vascular plants of the south. USDA, Forest Service, Tech. Publ. R8-TP2. 1305 pp. 07 Lammers, W.T. 1958. A study of certain environmental and physiological factors influencing the adaptation of three granite outcrop endemics: Amphianthus pusillus Torr., Isoetes melanospora Engelm., and Diamorpha cymosa (Nutt.) Briiton, Ph.D. Diss., Emory Univ., Atlanta, GA. 85 pp. 08 Lester, J.C. 1938. Geology of region around Stone Mountain, Georgia. Univ. of Calif. Studies 26:88-91. 09 Lunsford, D.E. 1938. Studies in the life cycle of Amphianthus pusillus Torr. A.A. Thesis, Emory Univ., Atlanta, GA. 88 pp. 10 McVaugh, R. 1943. The vegetation of the granite flatrocks of the Southeastern U.S. Ecol. Monog. 13:120-166. 11 McVaugh, R. and J.H. Pyron. 1937. The distribution of Amphianthus in Georgia. Castanea 2:104-105. 12 Miller, D.L. 1935. Report on a survey of Amphianthus pusillus (little amphianthus) in Albama. Provided under contract to: USFWS, Atlanta, GA. 33 pp. 13 Murdy, W.H. 1968. Plant speciation associated with granite outcrop communities of the Southeastern Piedmont. Rhodora 70:394-407. 14 Pennell, F.W. 1935. The Scrophulariaceae of Eastern Temperate North America. Acad. Nat. Sci. of Physiology Mong. No. 1, Philadelphia. 635 pp. 15 Randell, R.R. 1986. Electrophoectic study of the genetic variation in Amphianthus pusillus Torr. Unpubl. undergrad. research rpt., Emory Univ., Atlanta, GA. 20 pp. 16 Rayner, D.A. 1986. Report on the status of Amphianthus pusillus in South Carolina. Provided under contract to: USFWS, Southeast Region, Atlanta, GA. 33 pp. 17 U.S. Fish and Wildlife Service. 1986. Jackson Field Office Records. On file at: U.S. Fish and Wildl. Serv., 300 Woodrow Wilson Ave., Suite 316, Jackson, MS 39213. References - 1 (DRAFT) - References Species AMPHIANTHUS, LITTLE Species Id ESIS704051 Date 13 MAR 96 ***** REFERENCES FOR N-OCCURRENCE NARRATIVE ONLY ***** 01 Burbanck, M.P., and R.B. Platt. 1964. Granite outcrop communities of the Piedmont Plateau in Georgia. Ecology 45:292-306. 02 Garris, R.S. 1980. The endangered endemic, Amphianthus pusillus: a study of distribution and density. GA Dept. of Nat. Res., Protected Plants/Natural Areas Program. Unpubl. rept. 20 pp. 03 Harper, R.M. 1939. Granite outcrop vegetation in Alabama. Torreya 39:153-159. 04 Lunsford, D.E. 1938. Studies in the life cycle of Amphianthus pusillus Torr. A.A. Thesis, Emory Univ., Atlanta, GA. 88 pp. 05 McVaugh, R. 1943. The vegetation of the granite flatrocks of the Southeastern U.S. Ecol. Monog. 13:120-166. 06 McVaugh, R. and J.H. Pyron. 1937. The distribution of Amphianthus in Georgia. Castanea 2:104-105. 07 Miller, D.L. 1935. Report on a survey of Amphianthus pusillus (little amphianthus) in Albama. Provided under contract to: USFWS, Atlanta, GA. 33 pp. 08 Rayner, D.A. 1981. Granite flatrock outcrops in South Carolina. Bull. S.C. Acad. Sci. 43:106-107. 09 Rayner, D.A. 1986. Report on the status of Amphianthus pusillus in South Carolina. Provided under contract to: USFWS, Southeast Region, Atlanta, GA. 33 pp. 10 U.S. Fish and Wildlife Service. 1986. Jackson Field Office Records. On file at: U.S. Fish and Wildl. Serv., 300 Woodrow Wilson Ave., Suite 316, Jackson, MS 39213. References - 2