(DRAFT) - Taxonomy
                             Species grunion, California
                                 Species Id M010049
                                   Date 27 AUG 96



TAXONOMY

NAME - grunion, California OTHER COMMON NAMES - California grunion, grunion and smelt ELEMENT CODE - CATEGORY - Fish PHYLUM AND SUBPHYLUM - Chordata, CLASS AND SUBCLASS - Osteichthyes, ORDER AND SUBORDER - Atheriniformes, FAMILY AND SUBFAMILY - Atherinidae (Silversides), GENUS AND SUBGENUS - Leuresthes, SPECIES AND SSP - tenuis, SCIENTIFIC NAME - Leuresthes tenuis AUTHORITY - Ayres TAXONOMY REFERENCES - 65 Taxonomy - 1
                                  (DRAFT) - Status
                             Species grunion, California
                                 Species Id M010049
                                   Date 27 AUG 96



STATUS

Coded Status Sport Fish Commercial Existing, FMP exists Commercial/consumption Commercial/bait See Comments REFERENCES FOR STATUS - 65 COMMENTS ON STATUS - California grunion are caught by hand by sport fishermen during the open season when the fish spawn on beaches above the surf line at night. The commercial catch of grunion is taken incidentally while fishing for other species and is marketed fresh as "smelt". Limited quantities are used for live bait *65*. From 1927 to 1946, the fishing season in California was closed from April to June. Grunion may now be taken by sportsmen only by hand throughout the year except in April and May *65*. Status - 1
                               (DRAFT) - Distribution
                             Species grunion, California
                                 Species Id M010049
                                   Date 27 AUG 96



DISTRIBUTION

Distribution - 1
     

HABITAT ASSOCIATIONS

HABITAT - AQUATIC REFERENCES FOR HABITAT - 65 LAND USE - Water Bays and Estuaries Beaches REFERENCES FOR LAND USE - 65 NATIONAL WETLAND INVENTORY CODES NWI NWICLS NWIMOD NWISPEC Marine BB. S Marine BB. S Marine BB2 S Marine BB2 S REFERENCES FOR NWI - 65 COMMENTS ON HABITAT ASSOCIATIONS - The grunion has a unique spawning habit in that it spawns on land (on the beach) *65*. California grunion spawn every 4 weeks during the full moon tidal series. Grunion begin spawning two to six nights after the peak tides of each cycle, when each succeeding tide is lower than that of the previous night. Only three or four nights are suitable for spawning during each cycle. The spawning runs begin immediately after high tide *65*. ANIMAL/PLANT SPECIES ASSOCIATIONS - PARASITES: PARASITES Bomolachus pectinatus Caligus olsoni Clauellopsis Argulus melanostictus Nerocila californica Lironeca californica Metacercariae Asymphylodora atherinopsidis Lepocredium manteri REFERENCES FOR SPECIES ASSOCIATIONS - 65 COMMENTS ON SPECIES ASSOCIATIONS - PREDATION All life stages of grunion are preyed upon by a number of predators. Eggs buried in the beach sand are fed upon by sand worms, shore birds, and even ground squirrels. Shorebirds such as marbled godwit and whimbrel actively probe the sand in search of grunion eggs. An isopod, two species of flies, and a beetle have also been reported to prey on buried grunion eggs. Juvenile and adult grunion are preyed upon by halibut, sand bass, white croakers, and other large predators, including man *65*. PARASITES The grunion is the host of several crustaceans and digenetic trematoes. The six known crustacean parasites are the copepods Bomolachus pectinatus, Habitat Associations - 1 Caligus olsoni, and Clauellopsis; the brachiuran Argulus melanostictus; and the isopods Nerocila californica and Lironeca californica. Metacercariae with the charactieristics of Bucephalopsis lebiatus Trematoda: Bucephalida) infect the grunion heart muscle. Among the trematodes, Asymphylodora atherinopsidis occurs in the posterior intestine of the grunion while Lepocredium manteri occurs in the expanded anterior intestine. The effects of these parasites on growth and survival are not known *65*. Habitat Associations - 2
                                (DRAFT) - Food Habits
                             Species grunion, California
                                 Species Id M010049
                                   Date 27 AUG 96



FOOD HABITS

TROPHIC LEVEL - CARNIVORE REFERENCES FOR TROPHIC LEVEL - 65 LIFESTAGE FOOD FOOD PART Larva Protozoans Larva Crustaceans Larva stage Larva Copepods Larva stage Adult Other (Specified in Comments) Not Applicable Adult Zooplankton Not Applicable Adult Microcrustaceans Not Applicable REFERENCES FOR ADULT FOOD - 65 REFERENCES FOR LARVAE FOOD - 65 COMMENTS ON FOOD - Gut content analysis indicates that grunion feed primarily during the day *65*. COMMENTS ON ADULT FOOD - Adult grunion eat food similar to that eaten by larvae and juveniles. Stomach contents consist of microscopic and slightly larger planktonic organisms *65*. COMMENTS ON LARVAE FOOD - Food found in larvae less than 10 mm long consists largely of copepod nauplii and tintinnid protozoans. Cyclopoid copepods, Oithona sp., numerically dominate the gut contents of larvae 10-28 mm long. The harpactacoid copepod Euterpina acutifrons and the cyclopoid copepod Corycaeus anglicus are much less abundant. The relatively low densities of these animals in California's coastal waters suggest that most grunion larvae must search for prey *65*. Food Habits - 1
                         (DRAFT) - Environment Associations
                             Species grunion, California
                                 Species Id M010049
                                   Date 27 AUG 96



ENVIRONMENTAL ASSOCIATIONS

G = General A = Adult LIM = Limiting RA = Resting Adult J = Juvenile FA = Feeding Adult RJ = Resting Juvenile BA = Breeding Adult FJ = Feeding Juvenile P = Pupae L = Larvae E = Egg RL = Resting Larvae FL = Feeding Larvae
LIFESTAGE ENVIRONMENTAL ASSOCIATIONS LIM Water Temperature: Greater than 27 degrees C LIM Water Temperature: Between 21-27 degrees C LIM Water Temperature: Between 15-21 degrees C LIM Water Temperature: Below 15 degrees C LIM Water Temperature: Specified in Comments LIM BA Substrate: Sand BA Substrate: Specified in Comments BA Water Level: Specified in Comments BA Coastal Features: Specified in Comments BA Human Association: Specified in Comments BA Tidal inundation frequency/ duration: specified in comments E Water Temperature: Specified in Comments E Substrate: Sand E Substrate: Specified in Comments E Water Level: Specified in Comments E Coastal Features: Specified in Comments E Tidal inundation frequency/ duration: specified in comments REFERENCES FOR ENVIRONMENTAL ASSOC_ - 65 REFERENCES FOR LIMITING ENVIRONMENTAL ASSOC_ - 65 REFERENCES FOR BREEDING ADULT ENVIRONMENTAL ASSOC_ - 65 REFERENCES FOR EGG ENVIRONMENTAL ASSOC_ - 65 COMMENTS ON ENVIRONMENTAL ASSOCIATIONS - Young grunion are positively phototactic and can be attracted to light as bright as 10,000 lux. The strength of the gathering response is apparently related to the strength of the light stimulus *65*. COMMENTS ON LIMITING ENVIRONMENTAL ASSOC_ - WATER TEMPERATURE Water temperatures of 29.8 C and above reduce the viability of grunion larvae *65*. The ability of larvae to metabolize food (protein) decreases above 25 degrees C and below 16 degrees C (fat). Growth rates are positively correlated with temperature between 18 degrees and 25.4 degrees C *65*. Environment Associations - 1 (DRAFT) - Environment Associations Species grunion, California Species Id M010049 Date 27 AUG 96 SALINITY Only half of the grunion eggs placed in freshwater in one study hatched *65*. A decrease in salinity tolerance with age has been reported *65*. MOISTURE REQUIREMENTS Grunion eggs require moisture to prevent disiccation. Interstitial water (g water/kg sand) in grunion nests can range from 1% to 19% *65*. POWER PLANT EFFLUENTS An unidentified nonthermal component of power plant effluents significantly reduces hatching success of grunion. At all test temperatures in one study, the percentage of eggs hatched in effluent water was lower than the percentage hatched in sea water collected before passage through the power plant *65*. Exposure of eggs to benzo- (a)pyrene levels of 24 ppb or more decreased hatching percentage and increased morphological anomalies *65*. COMMENTS ON BREEDING ADULT ENVIRONMENTAL ASSOC_ - Grunion spawn on the beach. The female excavates the semifluid sand to bury herslf up to her pectoral fins. Occasionally females completely bury themselves in the nest *65*. California grunion are caught by hand by sport fishermen during the open season when the fish spawn on beaches above the surf line at night *65*. COMMENTS ON LARVAE ENVIRONMENTAL ASSOC_ - Newly hatched larvae usually live at or near the water surface *65*. Water temperatures of 29.8 C and above reduce the viability of grunion larvae. An increase of about 9 degrees C is required to double the development rate. Yolk-sac larvae have shown a preference for water temperature near 25 degrees C, well above the range that produces maximum growth. The ability of larvae to metabolize food (protein) decreases above 25 degrees C and below 16 degrees C (fat). Growth rates are positively correlated with temperature between 18 degrees and 25.4 degrees C *65*. Lower and upper lethal salinities for prolarvae have been found to be 4.2 and 41 ppt and for 20 day old post larvae these salinities were, respectively, 9 and 30 ppt *65*. COMMENTS ON EGG ENVIRONMENTAL ASSOC_ - TIDES Agitation is necessary because larval movement does not aid in escape from the egg shell. Seawater and agitation probably stimulate the release of an enzyme that softens the covering of the egg. Eggs do not usually hatch prematurely unless the eggs are washed free *65*. The evolution of egg size and yolk volume in grunion has apparently been influenced by the tides more than by temperature or any other factor *65*. In possible response to the more irregular tides of California, the eggs from the California grunion are much larger than those of their closest congener, Leuresthes sardina from the Gulf of California. Cyclic tides also deposit additional sand over the developing eggs. This provides protection from thermal, osmotic, and desiccation stresses as well as predation *65*. TEMPERATURE Grunion eggs hatch over a water temperature range of 14.0 to 28.5 degrees C *65*. SALINITY Environment Associations - 2 (DRAFT) - Environment Associations Species grunion, California Species Id M010049 Date 27 AUG 96 Only half of the grunion eggs placed in freshwater in one study hatched *65*. LIGHT Exposure to light seems to reduce hatching success of grunion eggs *65*. MOISTURE REQUIREMENTS Grunion eggs require moisture to prevent disiccation. Interstitial water (g water/kg sand) in grunion nests can range from 1% to 19% *65*. POWER PLANT EFFLUENTS An unidentified nonthermal component of power plant effluents significantly reduces hatching success of grunion. At all test temperatures in one study, the percentage of eggs hatched in effluent water was lower than the percentage hatched in sea water collected before passage through the power plant *65*. Environment Associations - 3
                               (DRAFT) - Life History
                             Species grunion, California
                                 Species Id M010049
                                   Date 27 AUG 96



LIFE HISTORY

MORPHOLOGY/IDENTIFICATION AIDS Dorsal fin V-VII + I, 9-10; anal fin I, 21-24; midlateral scales, 75; gill rakers 5-7 + 28-29; vertebrae 47-50. Body elongate and slender; eye diameter equal to snout length; maxillary not reaching pupil; premaxillary protrusible; jaw teeth lacking or minute; anal fin begins below 1st dorsal fin; scales between dorsal fins 7-9. Color in life: greenish above, with a silver-blue lateral stripe; silvery below; bluish blotch on cheek *65*. SPAWNING The grunion's unique spawning habits have long been recognized and it has been called the "fish that spawns on land". Through this unusual behavior, the California grunion has traded numerous marine perils for fewer terrestrial hazards, and thereby improved the probability of egg survivial. The spawning season begins in late February or early March and may extend to early September; peak spawning is in April and May. The protracted season may vary from year to year by several months. Most females in their third year of life (age-group II) begin spawning early in the season (April-May), whereas fish in their second year of life (age-group I) spawn somewhat later (May-June). About 30% of the females will have spawned in March, 75% by late April, and 90% by early May. The remaining 10% spawn from May to September. In late July, only 7% of the females examined contained mature ova, and in late August only immature ova were observed *65*. Grunion spawn as far north as New Brighton State Beach, Monterey Bay and as far south as Mexico. The largest grunion spawning runs typically are along the beaches in southern California *65*. In grunion spawning waters there are two high and two low tides daily. The higher of the high tides is at night in spring and summer. Each month, these higher tides reach their peak at the full and new moon. California grunion spawn every 4 weeks during the full moon tidal series. Apparently an individual grunion may spawn about every 2 weeks during both the full and new moon cycles. Female grunion are known to spawn as many as eight times a season on consecutive runs. Grunion begin spawning two to six nights after the peak tides of each cycle, when each succeeding tide is lower than that of the previous night. Only three or four nights are suitable for spawning during each cycle. The spawning runs begin immediately after high tide. At first, several fish, normally males, are swept up the beach and become temporarily stranded on the sand as they swim against the receding water. Gradually increasing numbers of both sexes beach themselves with succeeding waves. Spawning begins about 20 minutes after the first fish appear, peaks in one hour, and lasts 30 to 60 minutes. During peak spawning, thousands of grunion litter the beach. When the tide has ebbed about one foot, the night's run terminates as suddenly as it began *65*. Typically, females are accompanied by one or more males as they swim toward shore. Using a beach seine, twice as many males as females were captured during a spawning run in one study. If males are not present when the female is ready to spawn, she returns to the sea without laying eggs. During spawning, the female arches her body, keeping the head up while the caudal fin vigorously excavates the semifluid sand. As the tail sinks, the Life History - 1 (DRAFT) - Life History Species grunion, California Species Id M010049 Date 27 AUG 96 female twists her body and digs tail first until she is buried up to her pectoral fins. Occasionally females completely bury themselves in the nest. After the female is in the nest, up to eight males attempt to mate with her. The males curve around the female, placing their vents close to or in contact with her body. Concurrently, the female emits eggs 50 to 75 mm below the surface of the sand. The males discharge their milt into the sand near the female and immediately retreat toward the ocean. The milt flows down the female's body until it reaches the eggs below. The female then twists free and returns to the sea with the next wave. Generally about 30 seconds elapse from "nest" digging to egg laying but some fish remain on the beach for several minutes *65*. Waves tend to erode sand from the beach as the tide rises and to deposit sand as it falls; consequently, falling tides deposit more sand (41-46 cm) over previously buried eggs, which helps protect the eggs during low tides. The eggs remain in the sand for about 10 days, until the higher tide of the next lunar series erodes the sand and washes the eggs free. Eggs do not hatch until they are uncovered and agitated by the surf. Eggs of a single clutch hatch within 2 to 5 minutes. Agitation is necessary because larval movement does not aid in escape from the egg shell. Seawater and agitation probably stimulate the release of an enzyme that softens the covering of the egg. Eggs do not usually hatch prematurely unless the eggs are washed free. For example, eggs laid on a calm night were washed free the next night by high waves during a storm; the result was complete mortality *65*. FECUNDITY Fecundity is positively correlated with the size of the female. In various studies, eggs per nest have ranged from 1,149 to 3,600 *65*. OVA DEVELOPMENT Immediately after the fish spawn, a second maturing class of eggs begins differentiating and is spawned 15 days later. This 15-day cycle continues throughout the period of spawning; therefore, as the season progresses, a slight time lag is established between the 2-week tidal peaks and the spawning dates *65*. The California grunion egg is spherical and about 1.6 mm in diameter at hatching. The eggs lack the filaments attached to eggs of many atherinid species. Newly deposited eggs have many oil globules. The size and number vary during development until only one large uncolored oil globule remains in the yolk sac of the larva. The rate and duration of egg development is in synchrony with the tidal cycle *65*. LARVAE In contrast to larvae of other marine fishes, California grunion are large and well-developed when hatched. They are 6.5 to 6.8 mm long at hatching and 7.8 to 8.0 mm long after three days. Newly-hatched grunion are extremely active and the eyes and jaws are functional. The larvae are capable of immediate feeding but retain a yolk sac for 4 to 6 days. Laboratory feeding studies have shown that grunion larvae can live for a long time without food -- some as long as 3 weeks *65*. Newly hatched larvae usually live at or near the water surface. Larvae do not begin active feeding until the second day after hatching. Between the fifth and ninth day, swimming activity increases and they descent slightly below the surface film *65*. Life History - 2 (DRAFT) - Life History Species grunion, California Species Id M010049 Date 27 AUG 96 The young fish grow rapidly and are about 127 mm long at the end of their first year of life *65*. ADULTS Except for spawning habits and behavior, little is known about the adult stage in the coastal zone. Grunion populations seemingly move little along the shoreline, but seasonal inshore-offshore movements not associated with spawning are well-documented *65*. MATURITY AND LIFE SPAN Grunion mature in their second year of life and have a short life span. They apparently spawn in their second year of life (age group I). The largest grunion collected by one study was a 3-year-old male 170 mm long *(65)*. GROWTH CHARACTERISTICS Growth of grunion is most rapid during the first year of life and then declines in following years. At the end of the first year the mean total length is about 110 mm for males and 119 mm for females. After the second year's growth, average lengths are 129 and 140 mm. For the few fish reaching the beginning of a second spawning season, the average lengths have been found to be 143 mm (males) and 154 mm (females). The largest fish which has been reported was 191 mm long *65*. PREDATION All life stages of grunion are preyed upon by a number of predators. Eggs buried in the beach sand are fed upon by sand worms, shore birds, and even ground squirrels. Shorebirds such as marbled godwit and whimbrel actively probe the sand in search of grunion eggs. An isopod, two species of flies, and a beetle have also been reported to prey on buried grunion eggs. Juvenile and adult grunion are preyed upon by halibut, sand bass, white croakers, and other large predators, including man *65*. LIFE HISTORY CODES - Breeding/Spawning Season: February Breeding/Spawning Season: March Breeding Spawning Season: April Breeding/Spawning Season: May Breeding/Spawning Season: June Breeding/Spawning Season: July Breeding/Spawning Season: August Breeding/Spawning Season: September Mating System (Single breeding season): Polygamy (mal Mating System (Single breeding season): Promiscuity ( Duration of Pair Bond: No pair bond formed Spawning Site: Sand Nest Materials: Sand Gestation/Incubation Period: 1-2 weeks Average Number of Offspring/Reproductive Effort: 1,00 Number of Broods/Litters (Reproductive Efforts) Per Ye Periodicity: Active in day Life History - 3 (DRAFT) - Life History Species grunion, California Species Id M010049 Date 27 AUG 96 REFERENCES FOR LIFE HISTORY- 65 Life History - 4
                           (DRAFT) - Management Practices
                             Species grunion, California
                                 Species Id M010049
                                   Date 27 AUG 96



MANAGEMENT PRACTICES

RESULT MANAGEMENT PRACTICE Existing Regulating harvest of species being described Existing Regulating harvest - restricting weapons/gear use Existing Regulating harvest - setting seasons Existing Other management practices [specified in comments] Adverse Changing tidal cycle/ current patterns Adverse Salinity alteration Adverse Other management practices [specified in comments] Beneficial Controlling pollution [thermal, chemical, physical] Beneficial Other management practices [specified in comments] REFERENCES FOR BENEFICIAL MANAGEMENT PRACTICES - 65 REFERENCES FOR ADVERSE MANAGEMENT PRACTICES - 65 REFERENCES FOR EXISTING MANAGEMENT PRACTICES - 65 COMMENTS ON MANAGEMENT PRACTICES - COMMERCIAL AND SPORT FISHERY California grunion are caught by hand by sport fishermen during the open season when the fish spawn on beaches above the surf line at night. The commercial catch of grunion is taken incidentally while fishing for other species and is marketed fresh as "smelt". Limited quantities are used for live bait *65*. A few grunion are landed commercially by round haul nets and lamparas and are sold for bait or marketed as smelt in the fresh-fish market. Most grunion landed commercially are taken in late winter and in spring just before the spawning season *65*. Grunion are occasionally taken in pure hauls of up to 4 or 5 tons. Only then are grunion sold as fresh fish and then only if smelt are relatively scarce. Otherwise, the mixed catch is sold as bait or to canners of pet food *65*. REGULATION In the 1920's, the grunion fishery began showing signs of depletion , which was probably due to overfishing and habitat alteration. From 1927 to 1946, the fishing season in California was closed from April to June. Grunion may now be taken by sportsmen only by hand throughout the year except in April and May. Although the population size is unknown, the resource appears to be maintaining itself under the present sport fishing intensity. Since grunion populations seldom move laterally along the shoreline, local controls maybe adequate for management *65*. POWER PLANT EFFLUENTS An unidentified non-thermal component of power plant effluents significantly reduces hatching success of grunion. At all test temperatures in one study, the percentage of eggs hatched in effluent water was lower than the percentage hatched in sea water collected before passage through the power plant *65*. Management Practices - 1 (DRAFT) - Management Practices Species grunion, California Species Id M010049 Date 27 AUG 96 OTHER POLLUTANTS Exposure of eggs to benzo(a)pyrene levels of 24 ppb or more decreases hatching percentage and increases morphological anomalies *65*. SALINITY Only half of the grunion eggs placed in freshwater in one study hatched *65*. TIDES Agitation is necessary because larval movement does not aid in escape from the egg shell *65*. A decrease in salinity tolerance with age has been reported *65*. Management Practices - 2
                                   (DRAFT) - References
                               Species grunion, California
                                    Species Id M010049
                                      Date 27 AUG 96



     

References

65* Fritzsche, R.A., R.H. Chamberlain, R.A. Fisher. 1985. Species Profiles: Life Histories and Environmental Requirements of Coastal Fishes and Invertebrates (Pacific Southwest) -- California Grunion. U.S. Fish and Wildlife Service Biol. Rep. 82(11.28) pp 12. References - 1